Andrea Lazzarini, Antonio Macchiarulo, A. Floridi, Alice Coletti, Samuela Cataldi, Michela Codini, Remo Lazzarini, Elisa Bartoccini, Giacomo Cascianelli, Francesco Saverio Ambesi‐Impiombato, Tommaso Beccari, Francesco Curcio, Elisabetta Albi,
Lipid microdomains localized in the inner nuclear membrane are considered platforms for active chromatin anchoring. Stimuli such as surgery, vitamin D, or glucocorticoid drugs influence their gene expression, DNA duplication, and RNA synthesis. In this study, we used ultrafast liquid chromatography-tandem mass spectrometry to identify sphingomyelin (SM) species coupled with immunoblot analysis to comprehensively map differences in nuclear lipid microdomains (NLMs) purified from hepatocytes and hepatoma ...
Tópico(s): RNA Research and Splicing
2015 - American Society for Cell Biology | Molecular Biology of the Cell
Samuela Cataldi, Michela Codini, Giacomo Cascianelli, Sabina Tringali, Anna Tringali, Andrea Lazzarini, A. Floridi, Elisa Bartoccini, Mercedes Garcia‐Gil, Remo Lazzarini, Francesco Saverio Ambesi‐Impiombato, Francesco Curcio, Tommaso Beccari, Elisabetta Albi,
The action of dexamethasone is initiated by, and strictly dependent upon, the interaction of the drug with its receptor followed by its translocation into the nucleus where modulates gene expression. Where the drug localizes at the intranuclear level is not yet known. We aimed to study the localization of the drug in nuclear lipid microdomains rich in sphingomyelin content that anchor active chromatin and act as platform for transcription modulation. The study was performed in non-Hodgkin’s T cell ...
Tópico(s): Immune Cell Function and Interaction
2014 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Susanna Nuvoli, Barbara Palumbo, Mario Luca Fravolini, Bastiana Piras, Graziana Dachena, Tommaso Buresta, Silvia Cascianelli, Angela Spanu, Giuseppe Madeddu,
Tópico(s): Advanced MRI Techniques and Applications
2015 - Society of Nuclear Medicine and Molecular Imaging | Journal of Nuclear Medicine
Samuela Cataldi, Andrea Lazzarini, Michela Codini, Giacomo Cascianelli, A. Floridi, Elisa Bartoccini, Maria Rachele Ceccarini, Francesco Saverio Ambesi‐Impiombato, Tommaso Beccari, Francesco Curcio, Elisabetta Albi,
Abstract Nuclear actin has been implicated in different nuclear functions. In this work, its localization in nuclear membrane, chromatin and nuclear lipid microdomains was investigated. The implication of sphingomyelin metabolism was studied. Nuclear membrane, chromatin and nuclear lipid microdomains were purified from hepatocyte nuclei and H35 human hepatoma cell nuclei. The presence of β-actin was analyzed with immunoblotting by using specific antibodies. Sphingomyelinase, sphingomyelin-synthase, ...
Tópico(s): Erythrocyte Function and Pathophysiology
2017 - De Gruyter | The EuroBiotech Journal