Yoshinori Aoyama, Toshihiro Yamanari, Takurou N. Murakami, Tatsuya Nagamori, Kazuhiro Marumoto, Hiroto Tachikawa, Junji Mizukado, Hiroyuki Suda, Yūji Yoshida,
We investigated the initial photooxidation mechanism leading to reactive radical formation of polythiophene derivatives by focusing on the differences in the photochemical behaviors of photounstable poly(3-hexylthiophene) (P3HT) and photostable poly(3-octyloxythiophene) (P3OOT). Electron spin resonance measurements revealed that the [P3HT]+· (formed by oxygen doping) decayed, whereas no change was observed in the photostable [P3OOT]+· after light irradiation. Furthermore, the absorption decrease of the [ ...
Tópico(s): Synthesis and properties of polymers
2014 - Springer Nature | Polymer Journal
Yoshinori Aoyama, Toshihiro Yamanari, Nagatoshi Koumura, Hiroto Tachikawa, Masaru Nagai, Yūji Yoshida,
The photooxidation of poly(3-hexylthiophene) (P3HT) has previously been investigated and it is known that the sulfur atom of the thiophene ring is oxidized into sulfur oxides by a hydroxyl radical that arises through hydrogen abstraction at the hexyl side chain at the α-position. In this study, in order to further investigate the photooxidation mechanism of the polymer, the side chain at α-position was varied and three different polythiophene derivatives with phenyl (P3PhT), tert-pentyl (P3DMPT), and ...
Tópico(s): Synthesis and properties of polymers
2013 - Elsevier BV | Polymer Degradation and Stability
Jitian Zhang, Fengshi Chen, Xiangdong Zhao, Akihiro Aoyama, Toshihiro Okamoto, Takuji Fujinaga, Tsuyoshi Shoji, Hiroaki Sakai, Youbin Cui, Toru Bando, Hiroshi Date,
Tópico(s): Pulmonary Hypertension Research and Treatments
2008 - Elsevier BV | The Journal of Heart and Lung Transplantation
Yukimoto Maeda, Takafumi Aoyama, Toshihiro Odo, Satoru Nakai, Soju Suzuki,
The experimental fast reactor JOYO at the O-arai Engineering Center of the Japan Nuclear Cycle Development Institute is the first liquid sodium fast reactor in Japan. The purpose of constructing JOYO was to obtain technical information about liquid-metal fast breeder reactors (LMFBRs) through experience with their design, construction, and operation and to use the reactor as a fast neutron irradiation facility for the development of fuels, materials, and other components required for the LMFBR program. ...
Tópico(s): Nuclear and radioactivity studies
2005 - Taylor & Francis | Nuclear Technology
Ruben Bernardo, Diana V. Cunha, Can Wang, Leonel Pereira, Sónia Silva, Sara B. Salazar, Markus Schröder, Michiyo Okamoto, Azusa Takahashi‐Nakaguchi, Hiroji Chibana, Toshihiro Aoyama, Isabel Sá‐Correia, Joana Azeredo, Geraldine Butler, Nuno P. Mira,
Abstract To thrive in the acidic vaginal tract, Candida glabrata has to cope with high concentrations of acetic acid. The mechanisms underlying C. glabrata tolerance to acetic acid at low pH remain largely uncharacterized. In this work, the essential role of the CgHaa1 transcription factor (encoded by ORF CAGL0L09339g) in the response and tolerance of C. glabrata to acetic acid is demonstrated. Transcriptomic analysis showed that CgHaa1 regulates, directly or indirectly, the expression of about 75% ...
Tópico(s): Parasitic Infections and Diagnostics
2017 - Genetics Society of America | G3 Genes Genomes Genetics
Minoru Nagi, Koichi Tanabe, Keigo Ueno, Hironobu Nakayama, Toshihiro Aoyama, Hiroji Chibana, Satoshi Yamagoe, Takashi Umeyama, Takahiro Oura, Hideaki Ohno, Susumu Kajiwara, Yoshitsugu Miyazaki,
Summary During disseminated infection by the opportunistic pathogen C andida glabrata , uptake of sterols such as serum cholesterol may play a significant role during pathogenesis. The ATP ‐binding cassette transporter A us1p is thought to function as a sterol importer and in this study, we show that uptake of exogenous sterols occurred under anaerobic conditions in wild‐type cells of C . glabrata but not in AUS 1 ‐deleted mutant ( aus1 Δ) cells. In aerobic cultures, growth inhibition by fluconazole was ...
Tópico(s): Antibiotic Resistance in Bacteria
2013 - Wiley | Molecular Microbiology
Minoru Nagi, Hironobu Nakayama, Koichi Tanabe, Martin Bard, Toshihiro Aoyama, Makoto Okano, Satoru Higashi, Keigo Ueno, Hiroji Chibana, Masakazu Niimi, Satoshi Yamagoe, Takashi Umeyama, Susumu Kajiwara, Hideaki Ohno, Yoshitsugu Miyazaki,
Zn[2]-Cys[6] binuclear transcription factors Upc2p and Ecm22p regulate the expression of genes involved in ergosterol biosynthesis and exogenous sterol uptake in Saccharomyces cerevisiae. We identified two UPC2/ECM22 homologues in the pathogenic fungus Candida glabrata which we designated CgUPC2A and CgUPC2B. The contribution of these two genes to sterol homeostasis was investigated. Cells that lack CgUPC2A (upc2AΔ) exhibited enhanced susceptibility to the sterol biosynthesis inhibitors, fluconazole ...
Tópico(s): Fungal and yeast genetics research
2010 - Wiley | Genes to Cells
Hironobu Nakayama, Keigo Ueno, Jun Uno, Minoru Nagi, Koichi Tanabe, Toshihiro Aoyama, Hiroji Chibana, Martin Bard,
Farnesyl pyrophosphate (FPP) is utilized for many cellular processes, including the production of dolichols, ubiquinone (CoQ), sterols, farnesylated heme A and prenylated proteins. This lipid synthesized by FPP synthetase (ERG20) becomes attached to target proteins by the prenyltransferases, CDC43/RAM2 and RAM1/RAM2 complexes after the formation of the C15 and C20 units, respectively. Defects in protein prenylation as a result of inhibiting these enzyme complexes lead to pleiotropic effects in all ...
Tópico(s): RNA and protein synthesis mechanisms
2010 - Oxford University Press | FEMS Microbiology Letters
Hironobu Nakayama, Koichi Tanabe, Martin Bard, Wayne C. Hodgson, Sijia Wu, D. Takemori, Toshihiro Aoyama, N. Selvamuthu Kumaraswami, Laurel J. Metzler, Yukie Takano, Hiroji Chibana, Masakazu Niimi,
The uptake of endogenous sterol from serum may allow Candida glabrata to survive azole treatment. This study aims to determine the contribution of a sterol transporter that alters fluconazole sensitivity in the presence of serum. Bioinformatic analysis predicted CgAUS1 as the C. glabrata orthologue of the Saccharomyces cerevisiae transporters AUS1 and PDR11. To investigate whether the CgAUS1 gene has sterol transporter activity, we investigated the effects of an AUS1 deletion on the growth of a ...
Tópico(s): Peptidase Inhibition and Analysis
2007 - Oxford University Press | Journal of Antimicrobial Chemotherapy
Tamaki Cho, Toshihiro Aoyama, Mika Toyoda, Hironobu Nakayama, Hiroji Chibana, Hidenori Kaminishi,
Quorum sensing through farnesol, a quorum sensing molecule, regulates virulence and morphogenesis in Candida albicans. Farnesol and high cell density of C. albicans repress hyphal formation in a minimal medium containing N-acetyl-D-glucosamine. Global transcription profiling at an early stage of quorum sensing by C. albicans in the N-acetyl-D-glucosamine medium was analyzed. Twenty-two of a total of 53 genes responded to both farnesol and high cell density. From in silico analysis and previous published ...
Tópico(s): Bacterial biofilms and quorum sensing
2007 - | Nippon Ishinkin Gakkai Zasshi
Hiroji Chibana, Nao Oka, Hironobu Nakayama, Toshihiro Aoyama, B B Magee, P. T. Magee, Yuzuru Mikami,
The size of the genome in the opportunistic fungus Candida albicans is 15.6 Mb. Whole-genome shotgun sequencing was carried out at Stanford University where the sequences were assembled into 412 contigs. C. albicans is a diploid basically, and analysis of the sequence is complicated due to repeated sequences and to sequence polymorphism between homologous chromosomes. Chromosome 7 is 1 Mb in size and the best characterized of the 8 chromosomes in C. albicans. We assigned 16 of the contigs, ranging ...
Tópico(s): Plant-Microbe Interactions and Immunity
2005 - Oxford University Press | Genetics
Yoke Khin Yap, Toshihiro Aoyama, Yasaku Wada, Masashi Yoshimura, Yusuke Mori, T. Sasaki,
Cubic phase boron nitride (c-BN) film grown on intrinsic BN interlayer has been found to peel off near the interface. The strain force induced by the compressive c-BN film is suggested to extend the lattice spacing between the h-BN/t-BN (0002) planes, increase the lattice mismatch between BN phases and lead to the peeling of c-BN. Based on this concept, a new BN buffer layer enhancing c-BN adhesion has been grown. This buffer is almost stoichiometric in composition, tensile in stress and contains ...
Tópico(s): Boron and Carbon Nanomaterials Research
2000 - Elsevier BV | Diamond and Related Materials
Yoke Khin Yap, Toshihiro Aoyama, S. Kida, Yusuke Mori, T. Sasaki,
Cubic boron nitride (c-BN) thin films were prepared at 600 °C by radio-frequency (rf) plasma pulsed laser deposition. All c-BN films prepared in Ar-rich plasma have poor adhesion on Si(100) substrates, but those prepared in pure N2 plasma can be maintained for more than 5 months without degradation. However, an increase of ion flux at an ion energy similar to that of pure N2 plasma results in the peeling of c-BN films. Thus, application of pure N2 plasma with suppressed ion flux can improve c-BN film ...
Tópico(s): Boron and Carbon Nanomaterials Research
1999 - Elsevier BV | Diamond and Related Materials
Yoke Khin Yap, S. Kida, Toshihiro Aoyama, Yusuke Mori, T. Sasaki,
Carbon nitride (CN) thin films were prepared at 600 °C by rf plasma pulsed laser deposition. As we increased the magnitude of the negative dc bias voltage, the CN bonds were transformed from a mixture of sp2 C–N and sp3 C–N states into a CN phase predominated by tetrahedral CN bonds. A biasing threshold of this transformation occurred due to the annihilation of the graphite microstructure, which coincided with a threshold of significant nitrogen incorporation. We found that suppression of graphite ...
Tópico(s): Metal and Thin Film Mechanics
1998 - American Institute of Physics | Applied Physics Letters
Junichi Takagi, Toshihiro Aoyama, Shoko Ueki, Hirotsugu Ohba, Yuji Saito, L. Lóránd,
von Willebrand factor is a large multimeric plasma protein which plays important roles in platelet aggregation, blood coagulation and probably also in the adhesion of endothelial cells. A 100‐kDa propeptide, called the propolypeptide of von Willebrand factor (pp‐vWF), is generated during biosynthesis. We found that pp‐vWF served as a substrate for transglutaminases including human factor XIIIa and guinea pig liver transglutaminase [Usui, T., Takagi, J. & Saito, Y. (1993) J. Biol. Chem. 268 , 12311–123161. ...
Tópico(s): Blood Coagulation and Thrombosis Mechanisms
1995 - Wiley | European Journal of Biochemistry
Junichi Takagi, Tomoyuki Fujisawa, Tomoko Usui, Toshihiro Aoyama, Yuji Saito,
Location of the type V collagen-binding domain within bovine thrombospondin (TSP) was investigated by using fragments of reduced and alkylated TSP. A fragment of relative molecular mass (19 kDa) was isolated, which inhibited binding of 125I-TSP to type V collagen in a solid-phase binding assay. A direct binding assay using the 125I-labeled fragment confirmed that the fragment actually bound to collagen. The fragment retained specificity for the native structure of type V collagen like the intact TSP ...
Tópico(s): Collagen: Extraction and Characterization
1993 - Elsevier BV | Journal of Biological Chemistry
Toshio Mori, Masashi Watanabe, M. Horikawa, P. Nikaido, Hiroshi Kimura, Toshihiro Aoyama, Tsutomu Sugahara,
Although it is well known that WR-2721 is very efficient in protecting mice against lethal irradiation, we could not find any radioprotective effect of WR-2721 on mouse L cells in culture. But WR-1065 alone (free SH form of WR-2721), and WR-2721 pre-incubated with mouse liver homogenate, showed radioprotective ability. It was found that mouse liver homogenate dephosphorylated WR-2721 to WR-1065. The highest WR-2721 metabolizing activity was found in mouse liver homogenate and Chang liver cell homogenate. ...
Tópico(s): Plant Genetic and Mutation Studies
1983 - Taylor & Francis | International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine
Toshihiro Aoyama, Yoshimi Kamiyama, Shiro Usui, Román Blanco, Cecilia F. Vaquero, Pedro de la Villa,
Tópico(s): Photoreceptor and optogenetics research
2000 - Elsevier BV | Neuroscience Research
Yoke Khin Yap, S. Kida, Toshihiro Aoyama, Yusuke Mori, T. Sasaki,
Abstract We have obtained tetrahedral carbon nitride (CN) films by r.f. plasma pulsed laser deposition at 600 °C. As we increase the magnitude of the negative d.c. bias voltage of the Si substrate, predominant formation of tetrahedral CN bonds and suppression of graphite-like CN state are found. By means of such a bias voltage, CN films with adaptable fraction of graphite-like and tetrahedral CN bonds can be tailored according to requirement. All these films are stable to annealing at 800 °C. Likewise, ...
Tópico(s): Semiconductor materials and devices
1999 - Elsevier BV | Diamond and Related Materials
Tetsuya Matoba, Satoshi Yasuda, Koichi Kaikita, Masaharu Akao, Junya Ako, Masato Nakamura, Katsumi Miyauchi, Nobuhisa Hagiwara, Kazuo Kimura, Atsushi Hirayama, Kunihiko Matsui, Hisao Ogawa, Satoshi Yasuda, Hisao Ogawa, Kazuo Kimura, Nobuhisa Hagiwara, Atsushi Hirayama, Masato Nakamura, Katsumi Miyauchi, Junya Ako, Masaharu Akao, Koichi Kaikita, Tetsuya Matoba, Yukihiro Koretsune, Takafumi Hiro, Tetsuya Sumiyoshi, Kazumi Kimura, Yoichiro Hashimoto, Teruyuki Hirano, Hiroyuki Daida, Yasushi Okada, Tsutomu Yamazaki, Kunihiko Matsui, Hisao Ogawa, Kazuo Kimura, Nobuhisa Hagiwara, Atsushi Hirayama, Masato Nakamura, Katsumi Miyauchi, Junya Ako, Masaharu Akao, Koichi Kaikita, Tetsuya Matoba, Kunihiko Matsui, Akira Nakamura, Eiichi Tamiya, Takashi Yamamoto, Syuji Suetake, Teruo Noguchi, S. Nakamura, Akihiko Matsumura, Jisho Kojima, Satoru Suwa, H. Yamaguchi, Koichi Kaikita, Takanori Yasu, Asuka Nakajima, Takahisa Yamada, Hiroyuki Arai, Yoshiki Hata, Toshihiko Sakanashi, H Tateishi, Tôru Nakayama, Yoichi Nozaki, Masaharu Akao, Yasuo Okumura, Masahide Tokue, Norihiro Kuroki, Yuki Maruyama, Tetsuya Matoba, Noritsugu Hagiwara, Hiroshi Suzuki, Yasunori Nishida, Masayoshi Ajioka, Kazuhiko Yumoto, Shogo Shimizu, Takuma Aoyama, Hideki Shimomura, Toshihiro Takeda, Koichi Oshiro, Nobuyoshi Sugishita, Yuji Shibata, Takatoshi Otonari, Hajime Kihara, Hisao Ogawa, Ayako Ohno, M. Hazama, Makoto Shimizu, Kengo Tsukahara, S Haruta, Takatoshi Wakeyama, Tetsuya Haruna, Miwa Ito, Kenshi Fujii, Nobuhiko Atsuchi, Masafumi Sata, Kazuo Kimura, Naoyuki Hasebe, Yoshio Kobayasi, K. Ohsato, K. Hironaga, Yujiro Naganuma, Kazuhiro Anzaki, Koji Oiwa, Shinya Okazaki, Yoshihisa Nakagawa, K Tokuhiro, Kiyoshi Tanaka, Tomoyasu Momose, Yoshifumi Fukushima, Ryosuke Kametani, Katsunori Kawamitsu, Yuichi Saito, Shunsuke Akashi, Koji Kumagai, Koji Eshima, Tetsuya Tobaru, T. Seo, K Okuhara, Ken Kozuma, Yuji Ikari, Tomohiro Takahashi, Ichiro Michishita, Hisanori Fujikura, Shin‐ichi Momomura, Yuki Yamamoto, Kiyoshi Otomo, T. Matsubara, Hideki Tashiro, Teruo Inoue, Masaharu Ishihara, Ichiro Shiojima, Eizo Tachibana, Junya Ako, Kotaro Sumii, N. Yamamoto, Nobuhiro Ohmura, Toshihiro Nakamura, Yasuhiro Morita, Naomi Takahashi, Kentaro Watanabe, Hiroyuki Fujinaga, Masahiro Maruyama, Toru Oka, Takeshi Shirayama, T. Amano, Kazuki Fukui, Kenji Andò, Syuichi Oshima, Shuntaro Kagiyama, H. Teragawa, Masahiro Tahara, Shimato Ono, T. Koga, Katsuhito Fujiu, Masanari Kuwabara, Yusuke Ohya, Yosuke Yumoto, Naomitsu Kuji, Makoto Ikemura, Kazuomi Kario, Kenichi Chatani, Kōichi Sato, Hiroko Miyagi, Mamoru Murakami, Kiyoshi Saito, Masaaki Hoshiga, Seiichi Sato, Norifumi Kubo, Yuki Sakamoto, Kazuhiro Ashida, Hiroki Sakamoto, S. Murasaki, Hiroki Uehara, Takashi Akasaka, Y. Ooba, Shiro Nakahara, Yoichi Hanaoka, Takatoshi Nishimiya, Ryusuke Tsunoda, Y. Onuma, Sadanobu Higuchi, A Tani, Atsuyuki Wada, Masayuki Kato, Hiromi Obata, Yoshiharu Higuchi, Tsutomu Endo, R. Katou, T Matsunaga, T. Matsuoka, Hideaki Noguchi, Michio Usui, Takahiro Hayashi, Yutaka Otsuji, Takuya Osaki, Hirofumi Zaizen, Hiroyuki Yoshihara, Kazushige Kadota, Takanori Hirose, T. Miyazawa, A Mori, Misao Takano, Wataru Shimizu, Minoru Wake, Seizo Oriso, M. Yoshiyama, Shigeo Kakinoki, Toshikazu Nishioka, Takefumi Ozaki, K. Nomoto, K Seki, Kazuya Kawai, Yukio Ozaki, Shunsuke Miura, Masanori Kawasaki, Ryuichi Funada, Keigo Dote, Toshihiko Nagano, Shuichi Okamoto, Toshihiko Kubo, Yukichi Murozono, Takashi Owada, Tomohito Doke, Toshiyuki Matsumura, M. Horiuchi, Atsushi Takaishi, Masaki Yamamoto, Hitoshi Nakashima, Mitsuru Munemasa, Yasushi Sakata, Naoto Inoue, Tsuguhito Ota, Yuki Hamano, Nobutsugu Abe, T Tsubokura, M. Goto, I. Kubota, Masafumi Yano, Ken Umetani, Akihiko Matsumura, T Date, Hideo Morimoto, Toshiyuki Noda, Shinya Goto, Kiyoshi Hibi, Akira Nakano, Shinya Hiramitsu, Yasuki Kihara, M. Sugi, Nobuyuki Shiba, Daisuke Izumi, Takanori Sato, Sumiko Tayama, Toshiki Matsui, Atsushi Suzuki, Kohsuke Ajiki, M. Oishi, Michiro Kiryu, Toshinori Ko, Hideyuki Ando, Seiji Miyazaki, Toru Kinugawa, Hiromasa Otake, Hiroaki Kitaoka, Yoshihiro Hirata, Satoshi Honda, Mamoru Manita, Yasuhiro Ishii, Hideki Oka, Yūsuke Nanba, Masami Nishino, Tomohiro Sakamoto, Toshihiko Saito, Hiroshi Sakai, Mari Ichikawa, Shigeto Namiuchi, Koetsu Inoue, Nobuyuki Komiyama, Yoshihiro J. Akashi, Yuichi Nakamura, Tatsuya Komaru, T. Hosokawa, Taishiro Chikamori, Hiroyuki Tanaka, Osamu Arasaki, Kazutaka Aonuma, Yusuke Wakasa, Tomoharu Yoshizawa, Teruyasu Sugano, Naoto Yokota, Akira Kakutani, Tomohiro Suzuki, Yukihiko Abe, Toru Kataoka, Hideki Okayama,
The aim of this AFIRE (Atrial Fibrillation and Ischemic Events With Rivaroxaban in Patients With Stable Coronary Artery Disease) trial subgroup analysis was to examine rivaroxaban monotherapy benefits and their relation to the time between stenting and enrollment among patients after coronary stenting.Of 2,215 patients with atrial fibrillation and stable coronary artery disease in the AFIRE trial, rivaroxaban monotherapy was noninferior to rivaroxaban plus antiplatelet therapy (combination therapy) ...
Elsevier BV
Yoshinori Aoyama, Toshihiro Yamanari, Noboru Ohashi, Yosei Shibata, Yasumasa Suzuki, Junji Mizukado, Hiroyuki Suda, Yūji Yoshida,
During the photoirreversible degradation of a polymer solar cell (PSC), it is generally assumed that the photooxidation of the organic materials (P3HT, PCBM) affects the device performance. Photooxidation of the organic materials results in a decrease in absorbance as a result of photodecomposition. However, the effect of initial photooxidation on device performance remains to be investigated. In this study, we try to establish the correlation between the initial photooxidation of the organic material ...
Tópico(s): Thin-Film Transistor Technologies
2013 - Elsevier BV | Solar Energy Materials and Solar Cells
Kuniko Mitamura, Eriko Aoyama, Toshihiro Sakai, Takashi Iida, Alan F. Hofmann, Shigeo Ikegawa,
Tópico(s): Carbohydrate Chemistry and Synthesis
2011 - Springer Science+Business Media | Analytical and Bioanalytical Chemistry
Toshihiro Aoyama, Hironobu Nakayama, Keigo Ueno, Tatsuya Inukai, Koichi Tanabe, Minoru Nagi, Martin Bard, Hiroji Chibana,
DNA sequencing of the 5′‐flanking region of the transcriptome effectively identifies transcription initiation sites and also aids in identifying unknown genes. This study describes a comprehensive polling of transcription start sites and an analysis of full‐length complementary DNA s derived from the genome of the pathogenic fungus Candida glabrata . A comparison of the sequence reads derived from a cDNA library prepared from cells grown under different culture conditions against the reference genomic ...
Tópico(s): Plant-Microbe Interactions and Immunity
2014 - Wiley | Genes to Cells
Yoke Khin Yap, Y. Mori, S. Kida, Toshihiro Aoyama, T. Sasaki,
Carbon nitride (CN) thin films were synthesized at 600°C. Despite the high synthesis temperature, the nitrogen content of CN films was observed to increase with the increase of negative DC bias voltage of Si substrate as determined by X-ray photoelectron spectroscopy. Transformation of CN binding states was found to proceed with the increase of bias voltage as indicated by a series of Fourier transform infrared spectra. These films were dominated by tetrahedral sp3 C–N binding state at a substrate ...
Tópico(s): Metal and Thin Film Mechanics
1999 - Elsevier BV | Journal of Crystal Growth
Tatsumi Aoyama, Toshihiro Matsuo, Y. Shibusa,
We apply an improved Taylor expansion method, which is a variational scheme to the Ising model in two dimensions. This method enables us to evaluate the free energy and magnetization in strong coupling regions from the weak coupling expansion, even in the case of a phase transition. We determine the approximate value of the transition point using this scheme. In the presence of an external magnetic field, we find both stable and metastable physical states.
Tópico(s): Quantum Chromodynamics and Particle Interactions
2006 - Oxford University Press | Progress of Theoretical Physics
Madoka Matsumoto-Ida, Y Takimoto, Takeshi Aoyama, Masaharu Akao, Toshihiro Takeda, Toru Kita,
Transforming growth factor-beta1 (TGF-beta1) alters myocardial gene expression, resulting in myocyte hypertrophy, through activation of TGF-beta-activated kinase (TAK1), a member of the mitogen-activated protein kinase kinase kinase (MAPKKK) family. We hypothesized that the TGF-beta1-TAK1-p38 MAPK pathway might be activated during ventricular remodeling after myocardial infarction (MI). One, 3, 7, and 14 days after ligation of the left anterior descending coronary artery, noninfarcted left ventricular ...
Tópico(s): TGF-β signaling in diseases
2005 - American Physical Society | AJP Heart and Circulatory Physiology
T. AOYAMA, Kensuke Fukui, Toshihiro Nakamori, Yukio Hashimoto, Takashi Yamamoto, Kiyoharu Takamatsu, Michihiro Sugano,
The effect on genetically obese mice of a milk whey protein isolate (WPI) and soy protein isolate (SPI) and their hydrolysates (WPI-H, SPI-H) on the rate of body fat disappearance was investigated. Male yellow KK mice were made obese by feeding with a high-fat diet containing 30% fat from 6 to 10 weeks of age. They were then fed with an energy-restricted low fat (5.0%) and high protein (35% WPI, WPI-H, SPI or SPI-H) diet for 2 weeks at the 60% level of energy intake by mice on laboratory feed. During ...
Tópico(s): Nutrition, Genetics, and Disease
2000 - Oxford University Press | Bioscience Biotechnology and Biochemistry
K. Aoyama, K. Nakagawa, Toshihiro Itoh,
This paper describes the theoretical and experimental study of an optical time domain reflectometry (OTDR) in a single-mode fiber. The maximum measurable distance by the OTDR is theoretically studied. In the experiment, the cylindrical lens attached to the laser diode, the low-loss and low-crosstalk directional coupler, and the digital averaging technique combined enabled us to measure a break in a single-mode fiber up to 17 km in length. This paper also describes the application of the OTDR to loss ...
Tópico(s): Optical Network Technologies
1981 - IEEE Photonics Society | IEEE Journal of Quantum Electronics
Michiyo Okamoto, Azusa Takahashi‐Nakaguchi, Kengo Tejima, Kaname Sasamoto, Masashi Yamaguchi, Toshihiro Aoyama, Minoru Nagi, Kohichi Tanabe, Yoshitsugu Miyazaki, Hironobu Nakayama, Chihiro Sasakawa, Susumu Kajiwara, Alistair J. P. Brown, Miguel C. Teixeira, Hiroji Chibana,
The uptake of cholesterol from the host is closely linked to the proliferation of pathogenic fungi and protozoa during infection. For some pathogenic fungi , cholesterol uptake is an important strategy for decreasing susceptibility to antifungals that inhibit ergosterol biosynthesis. In this study, we show that Candida glabrata ERG25 , which encodes an enzyme that demethylates 4,4-dimethylzymosterol, is required for cholesterol uptake from host serum. Based on the screening of C. glabrata conditional ...
Tópico(s): Microbial Natural Products and Biosynthesis
2022 - Frontiers Media | Frontiers in Cell and Developmental Biology
Tatsuya Inukai, Minoru Nagi, Akihiro Morita, Koichi Tanabe, Toshihiro Aoyama, Yoshitsugu Miyazaki, Martin Bard, Hironobu Nakayama,
Sterol uptake in the pathogenic fungus, Candida glabrata, occurs via the sterol transporter, CgAus1p. Azole inhibition of sterol biosynthesis can under certain circumstances be reversed by adding exogenously sterol. Here we demonstrate that the CgTIR3 (CAGL0C03872g) gene product is also required for sterol uptake, since Cgtir3Δ strains fail to take up sterol both aerobically and under hypoxic conditions. Western analysis using an HA-tagged TIR3 strain showed that CgTir3p localizes to the cell wall, ...
Tópico(s): Fungal Biology and Applications
2014 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids