Jun Nakae, Yongheng Cao, Fumihiko Hakuno, Hiroshi Takemori, Yoshinaga Kawano, Risa Sekioka, Takaya Abe, Hiroshi Kiyonari, Toshiya Tanaka, Juro Sakai, Shin‐Ichiro Takahashi, Hiroshi Itoh,
... Japan Search for more papers by this author Toshiya Tanaka Toshiya Tanaka Division of metabolic medicine; Research Center for Advanced ... Japan Search for more papers by this author Toshiya Tanaka Toshiya Tanaka Division of metabolic medicine; Research Center for Advanced ...
Tópico(s): Plant Molecular Biology Research
2012 - Springer Nature | The EMBO Journal
Kabuto Takano, Go Hasegawa, Shuying Jiang, Isao Kurosaki, Katsuyoshi Hatakeyama, Hiroko Iwanari, Toshiya Tanaka, Takao Hamakubo, Tatsuhiko Kodama, Makoto Naito,
... JapanSearch for more papers by this authorToshiya Tanaka, Toshiya Tanaka Laboratory for Systems Biology and Medicine, Research Center ... JapanSearch for more papers by this authorToshiya Tanaka, Toshiya Tanaka Laboratory for Systems Biology and Medicine, Research Center ...
Tópico(s): Genetic factors in colorectal cancer
2009 - Wiley | Pathology International
Ryûji Yamamoto, Tsuyoshi Osawa, Yusuke Sasaki, Shogo Yamamoto, Motonobu Anai, Kouji Izumi, Yoshihiro Matsumura, Juro Sakai, Hiroyuki Aburatani, Atsushi Mizokami, Tatsuhiko Kodama, Toshiya Tanaka,
... 2 , Atsushi Mizokami 3 , Tatsuhiko Kodama 1 and Toshiya Tanaka 1 1 Laboratory for Systems Biology and Medicine ( ... of Medicine, Sendai 980-8574, Japan Correspondence to: Toshiya Tanaka, email: tanaka@lsbm.org Keywords: CRPC; neuroendocrine prostate ...
Tópico(s): Hippo pathway signaling and YAP/TAZ
2018 - Impact Journals LLC | Oncotarget
Akira Sato, Shosei Kishida, Toshiya Tanaka, Akira Kikuchi, Tatsuhiko Kodama, Makoto Asashima, Ryuichi Nishinakamura,
The Spalt (sal) gene family plays an important role in regulating developmental processes of many organisms. Mutations of human SALL1 cause the autosomal dominant disorder, Townes–Brocks syndrome (TBS), and result in ear, limb, anal, renal, and heart anomalies. Targeted deletion of mouse Sall1 results in kidney agenesis or severe dysgenesis. Molecular mechanisms of Sall1, however, have remained largely unknown. Here we report that Sall1 synergistically activates canonical Wnt signaling. The transcriptional ...
Tópico(s): Genetic Syndromes and Imprinting
2004 - Elsevier BV | Biochemical and Biophysical Research Communications
Oronza A. Botrugno, Elisabeth Fayard, Jean‐Sébastien Annicotte, Céline Haby, Thomas J. Brennan, Olivia Wendling, Toshiya Tanaka, Tatsuhiko Kodama, Winston Thomas, Johan Auwerx, Kristina Schoonjans,
LRH-1 is an orphan nuclear receptor predominantly expressed in tissues of endodermal origin, where it controls development and cholesterol homeostasis. We show here that LRH-1 induces cell proliferation through the concomitant induction of cyclin D1 and E1, an effect that is potentiated by its interaction with β-catenin. Whereas β-catenin coactivates LRH-1 on the cyclin E1 promoter, LRH-1 acts as a potent tissue-restricted coactivator of β-catenin on the cyclin D1 promoter. The implication of LRH- ...
Tópico(s): Epigenetics and DNA Methylation
2004 - Elsevier BV | Molecular Cell
Takashi Maejima, Tsuyoshi Inoue, Yasuharu Kanki, Takahide Kohro, Guoliang Li, Yoshihiro Ohta, Hiroshi Kimurâ, Mika Kobayashi, Akashi Taguchi, Shuichi Tsutsumi, Hiroko Iwanari, Shogo Yamamoto, Hirofumi Aruga, Shoulian Dong, Junko Stevens, Huay Mei Poh, Kazuki Yamamoto, Takeshi Kawamura, Imari Mimura, Jun-ichi Suehiro, Akira Sugiyama, Kiyomi Kaneki, Haruki Shibata, Yasunobu Yoshinaka, Takeshi Doi, Akimune ASANUMA, Sohei Tanabe, Toshiya Tanaka, Takashi Minami, Takao Hamakubo, Juro Sakai, Naohito Nozaki, Hiroyuki Aburatani, Masaomi Nangaku, Xiaoan Ruan, Hideyuki Tanabe, Yijun Ruan, Sigeo Ihara, Akira Endo, Tatsuhiko Kodama, Youichiro Wada,
Statins exert atheroprotective effects through the induction of specific transcriptional factors in multiple organs. In endothelial cells, statin-dependent atheroprotective gene up-regulation is mediated by Kruppel-like factor (KLF) family transcription factors. To dissect the mechanism of gene regulation, we sought to determine molecular targets by performing microarray analyses of human umbilical vein endothelial cells (HUVECs) treated with pitavastatin, and KLF4 was determined to be the most highly ...
Tópico(s): Peroxisome Proliferator-Activated Receptors
2014 - Public Library of Science | PLoS ONE
Takeshi Inagaki, Satoshi Iwasaki, Yoshihiro Matsumura, Takeshi Kawamura, Toshiya Tanaka, Yohei Abe, Ayumu Yamasaki, Yuya Tsurutani, Ayano Yoshida, Yoko Chikaoka, Kanako Nakamura, Kenta Magoori, Ryo Nakaki, Timothy F. Osborne, Kiyoko Fukami, Hiroyuki Aburatani, Tatsuhiko Kodama, Juro Sakai,
Polycomb repressive complex 1 (PRC1) plays an essential role in the epigenetic repression of gene expression during development and cellular differentiation via multiple effector mechanisms, including ubiquitination of H2A and chromatin compaction. However, whether it regulates the stepwise progression of adipogenesis is unknown. Here, we show that FBXL10/KDM2B is an anti-adipogenic factor that is up-regulated during the early phase of 3T3-L1 preadipocyte differentiation and in adipose tissue in ...
Tópico(s): Histone Deacetylase Inhibitors Research
2014 - Elsevier BV | Journal of Biological Chemistry
Tsuyoshi Inoue, Takahide Kohro, Toshiya Tanaka, Yasuharu Kanki, Guoliang Li, Huay-Mei Poh, Imari Mimura, Mika Kobayashi, Akashi Taguchi, Takashi Maejima, Jun‐ichi Suehiro, Akira Sugiyama, Kiyomi Kaneki, Hirofumi Aruga, Shoulian Dong, Junko Stevens, Shogo Yamamoto, Shuichi Tsutsumi, Toshiro Fujita, Xiaoan Ruan, Hiroyuki Aburatani, Masaomi Nangaku, Yijun Ruan, Tatsuhiko Kodama, Youichiro Wada,
Synergistic transcriptional activation by different stimuli has been reported along with a diverse array of mechanisms, but the full scope of these mechanisms has yet to be elucidated.We present a detailed investigation of hypoxia-inducible factor (HIF) 1 dependent gene expression in endothelial cells which suggests the importance of crosstalk between the peroxisome proliferator-activated receptor (PPAR) β/δ and HIF signaling axes. A migration assay shows a synergistic interaction between these two ...
Tópico(s): Peroxisome Proliferator-Activated Receptors
2014 - BioMed Central | Genome biology
Toshiya Tanaka, Satoko Tahara‐Hanaoka, Tsukasa Nabekura, Kaori Ikeda, Shuying Jiang, Shuichi Tsutsumi, Takeshi Inagaki, Kenta Magoori, Takuma Higurashi, Hirokazu Takahashi, Keisuke Tachibana, Yuya Tsurutani, Sana Raza, Motonobu Anai, Takashi Minami, Youichiro Wada, Koutaro Yokote, Takefumi Doi, Takao Hamakubo, Johan Auwerx, Frank J. Gonzalez, Atsushi Nakajima, Hiroyuki Aburatani, Makoto Naito, Akira Shibuya, Tatsuhiko Kodama, Juro Sakai,
Abstract Macrophages are important for maintaining intestinal immune homeostasis. Here, we show that PPARβ/δ (peroxisome proliferator-activated receptor β/δ) directly regulates CD300a in macrophages that express the immunoreceptor tyrosine based-inhibitory motif (ITIM)-containing receptor. In mice lacking CD300a, high-fat diet (HFD) causes chronic intestinal inflammation with low numbers of intestinal lymph capillaries and dramatically expanded mesenteric lymph nodes. As a result, these mice exhibit ...
Tópico(s): Adipokines, Inflammation, and Metabolic Diseases
2014 - Nature Portfolio | Scientific Reports
Akira Shiono, Hiroki Sasaki, Reio Sekine, Yohei Abe, Yoshihiro Matsumura, Takeshi Inagaki, Toshiya Tanaka, Tatsuhiko Kodama, Hiroyuki Aburatani, Juro Sakai, Hitoshi Takagi,
Abstract Two large clinical studies showed that fenofibrate, a commonly used peroxisome proliferator-activated receptor α (PPARα) agonist, has protective effects against diabetic retinopathy. However, the underlying mechanism has not been clarified. We performed genome-wide analyses of gene expression and PPARα binding sites in vascular endothelial cells treated with the selective PPARα modulator pemafibrate and identified 221 target genes of PPARα including THBD , which encodes thrombomodulin (TM). ...
Tópico(s): Immune cells in cancer
2020 - Nature Portfolio | Scientific Reports
Yusuke Sasaki, Masato Asahiyama, Toshiya Tanaka, Shogo Yamamoto, Kentaro Murakami, Wakana Kamiya, Yoshihiro Matsumura, Tsuyoshi Osawa, Motonobu Anai, Jean‐Charles Fruchart, Hiroyuki Aburatani, Juro Sakai, Tatsuhiko Kodama,
Non-alcoholic steatohepatitis (NASH) is characterized by macrovesicular steatosis with ballooning degeneration of hepatocytes, diffused lobular inflammation, and fibrosis. PPAR ligands are promising therapeutic agents in NASH; accordingly, we evaluated the effects of the first clinically available selective PPARα modulator, pemafibrate. We found that pemafibrate improves F4/80-positive macrophage accumulation, ballooning degeneration of hepatocytes, and the non-alcoholic fatty liver disease (NAFLD) ...
Tópico(s): Diet, Metabolism, and Disease
2020 - Nature Portfolio | Scientific Reports
Masashi Okamura, Hiromi Kudo, Ken‐ichi Wakabayashi, Toshiya Tanaka, Aya Nonaka, Aoi Uchida, Shuichi Tsutsumi, Iori Sakakibara, Makoto Naito, Timothy F. Osborne, Takao Hamakubo, Sadayoshi Ito, Hiroyuki Aburatani, Masashi Yanagisawa, Tatsuhiko Kodama, Juro Sakai,
Wnt signaling through beta-catenin and TCF maintains preadipocytes in an un-differentiated proliferative state; however, the molecular pathway has not been completely defined. By integrating gene expression microarray, chromatin immunoprecipitation-chip, and cell-based experimental approaches, we show that Wnt/beta-catenin signaling activates the expression of COUP-TFII which recruits the SMRT corepressor complex to the first introns located downstream from the first exons of both PPARgamma1 and ...
Tópico(s): RNA modifications and cancer
2009 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Kenji Ishimoto, Hiroki Nakamura, Keisuke Tachibana, Daisuke Yamasaki, Akemi Ota, Ken‐ichi Hirano, Toshiya Tanaka, Takao Hamakubo, Juro Sakai, Tatsuhiko Kodama, Takefumi Doi,
Lipin 1 plays a crucial role in lipid metabolism in adipose tissue, skeletal muscle, and liver. Its physiological role involves two cellular functions: regulation of phosphatidate phosphatase activity and regulation of fatty acid oxidation. In this study, we have demonstrated that lipin 1 gene (LPIN1) expression is regulated by cellular sterols, which are key regulators of lipid metabolism. We have also characterized the sterol-response element and nuclear factor Y-binding sites in the human LPIN1 ...
Tópico(s): Peroxisome Proliferator-Activated Receptors
2009 - Elsevier BV | Journal of Biological Chemistry
Keisuke Tachibana, Kentaro Takeuchi, Hirohiko Inada, Daisuke Yamasaki, Kenji Ishimoto, Toshiya Tanaka, Takao Hamakubo, Juro Sakai, Tatsuhiko Kodama, Takefumi Doi,
Solute carrier family 25, member 20 (SLC25A20) is a key molecule that transfers acylcarnitine esters in exchange for free carnitine across the mitochondrial membrane in the mitochondrial β-oxidation. The peroxisome proliferator-activated receptor alpha (PPARα) is a ligand-activated transcription factor that plays an important role in the regulation of β-oxidation. We previously established tetracycline-regulated human cell line that can be induced to express PPARα and found that PPARα induces the ...
Tópico(s): Adipose Tissue and Metabolism
2009 - Elsevier BV | Biochemical and Biophysical Research Communications
Iori Sakakibara, Takahiro Fujino, Makoto Ishii, Toshiya Tanaka, Tatsuo Shimosawa, Shinji Miura, Wei Zhang, Yuka Tokutake, Joji Yamamoto, Mutsumi Awano, Satoshi Iwasaki, Toshiyuki Motoike, Masashi Okamura, Takeshi Inagaki, Kiyoshi Kita, Osamu Ezaki, Makoto Naito, Tomoyuki Kuwaki, Shigeru Chohnan, Tokuo Yamamoto, Robert E. Hammer, Tatsuhiko Kodama, Masashi Yanagisawa, Juro Sakai,
Acetate is activated to acetyl-CoA by acetyl-CoA synthetase 2 (AceCS2), a mitochondrial enzyme. Here, we report that the activation of acetate by AceCS2 has a specific and unique role in thermogenesis during fasting. In the skeletal muscle of fasted AceCS2−/− mice, ATP levels were reduced by 50% compared to AceCS2+/+ mice. Fasted AceCS2−/− mice were significantly hypothermic and had reduced exercise capacity. Furthermore, when fed a low-carbohydrate diet, 4-week-old weaned AceCS2−/− mice also exhibited ...
Tópico(s): Mitochondrial Function and Pathology
2009 - Cell Press | Cell Metabolism
Ken‐ichi Wakabayashi, Masashi Okamura, Shuichi Tsutsumi, Naoko Nishikawa, Toshiya Tanaka, Iori Sakakibara, Jun-ichi Kitakami, Sigeo Ihara, Yuichi Hashimoto, Takao Hamakubo, Tatsuhiko Kodama, Hiroyuki Aburatani, Juro Sakai,
Control of cell differentiation occurs through transcriptional mechanisms and through epigenetic modification. Using a chromatin immunoprecipitation-on-chip approach, we performed a genome-wide search for target genes of peroxisome proliferator-activated receptor gamma (PPAR gamma) and its partner protein retinoid X receptor alpha during adipogenesis. We show that these two receptors target several genes that encode histone lysine methyltransferase SET domain proteins. The histone H4 Lys 20 (H4K20) ...
Tópico(s): Cancer-related gene regulation
2009 - Taylor & Francis | Molecular and Cellular Biology
Takeshi Inagaki, Makoto Tachibana, Kenta Magoori, Hiromi Kudo, Toshiya Tanaka, Masashi Okamura, Makoto Naito, Tatsuhiko Kodama, Yoichi Shinkai, Juro Sakai,
Histone H3 lysine 9 (H3K9) methylation is a crucial epigenetic mark of heterochromatin formation and transcriptional silencing. Recent studies demonstrated that most covalent histone lysine modifications are reversible and the jumonji C (JmjC)-domain-containing proteins have been shown to possess such demethylase activities. However, there is little information available on the biological roles of histone lysine demethylation in intact animal model systems. JHDM2A (JmjC-domain-containing histone demethylase ...
Tópico(s): Histone Deacetylase Inhibitors Research
2009 - Wiley | Genes to Cells
Youichiro Wada, Akira Sugiyama, Takashi Yamamoto, Makoto Naito, Noriko Noguchi, Shinji Yokoyama, Maki Tsujita, Yoshiki Kawabe, Mika Kobayashi, Akashi Izumi, Takahide Kohro, Toshiya Tanaka, Hirokazu Taniguchi, Hidenori Koyama, Ken‐ichi Hirano, Shizuya Yamashita, Yūji Matsuzawa, Etsuo Niki, Takao Hamakubo, Tatsuhiko Kodama,
The effect of a variety of hypoxic conditions on lipid accumulation in smooth muscle cells (SMCs) was studied in an arterial wall coculture and monocultivation model.Low density lipoprotein (LDL) was loaded under various levels of oxygen tension. Oil red O staining of rabbit and human SMCs revealed that lipid accumulation was greater under lower oxygen tension. Cholesterol esters were shown to accumulate in an oxygen tension-dependent manner by high-performance liquid chromatographic analysis. Autoradiograms ...
Tópico(s): Adipokines, Inflammation, and Metabolic Diseases
2002 - Lippincott Williams & Wilkins | Arteriosclerosis Thrombosis and Vascular Biology
Toshiya Tanaka, Tetsu Takeno, Yuichiro Watanabe, Yasutoshi Uchiyama, Takeshi Murakami, Hisahiko Yamashita, Akifumi Suzuki, Rie Aoi, Hiroko Iwanari, Shu-Ying Jiang, Makoto Naito, Keisuke Tachibana, Takefumi Doi, Andrew I. Shulman, David J. Mangelsdorf, Raphael Reiter, Johan Auwerx, Takao Hamakubo, Tatsuhiko Kodama,
Monoclonal antibodies (Mabs) are valuable reagents for the purification, characterization and immunolocalization of proteins. In this study, we raised Mabs against human peroxisome proliferator-activated receptors (PPARs) using baculovirus particles displaying surface glycoprotein gp64-fusion proteins as the immunizing agent. In this system, to display fusion proteins on the viral surface, the amino terminal sequences of human PPARδ and PPARγ2 are inserted in-frame between the signal sequence and ...
Tópico(s): Viral Infectious Diseases and Gene Expression in Insects
2002 - Japan Atherosclerosis Society | Journal of Atherosclerosis and Thrombosis
Yasuhiro Yagi, Hitoshi Kishi, R. Hagihara, Toshiya Tanaka, Shinichi Kozuma, T. Ishida, M. Waki, Makoto TANAKA, Seiichi Kiyama,
Diagnostic technology for photovoltaic (PV) systems was developed, using the learning method to take each site’s conditions into account. This technology employs diagnostic criteria databases to analyze data acquired from the PV systems. These criteria are updated monthly for each site using analyzed data. To check the shadows on the PV modules and pyranometer, the sophisticated verification method was also applied to this technology. After the diagnosis, a basket method provides maintenance advice ...
Tópico(s): Power Systems and Renewable Energy
2003 - Elsevier BV | Solar Energy Materials and Solar Cells
Toshiya Tanaka, Joji Yamamoto, Satoshi Iwasaki, Hiroshi Asaba, Hiroki Hamura, Yukio Ikeda, Mitsuhiro Watanabe, Kenta Magoori, Ryoichi X. Ioka, Keisuke Tachibana, Yuichiro Watanabe, Yasutoshi Uchiyama, Koichi Sumi, Haruhisa Iguchi, Sadayoshi Ito, Takefumi Doi, Takao Hamakubo, Makoto Naito, Johan Auwerx, Masashi Yanagisawa, Tatsuhiko Kodama, Juro Sakai,
In this study, we defined the role of peroxisome proliferator-activated receptor β/δ (PPARδ) in metabolic homeostasis by using subtype selective agonists. Analysis of rat L6 myotubes treated with the PPARδ subtype-selective agonist, GW501516, by the Affymetrix oligonucleotide microarrays revealed that PPARδ controls fatty acid oxidation by regulating genes involved in fatty acid transport, β-oxidation, and mitochondrial respiration. Similar PPARδ-mediated gene activation was observed in the skeletal ...
Tópico(s): Metabolism, Diabetes, and Cancer
2003 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Shuying Jiang, Toshiya Tanaka, Hiroko Iwanari, Hiromitsu Hotta, Hisahiko Yamashita, Junko Kumakura, Yuichiro Watanabe, Yasutoshi Uchiyama, Hiroyuki Aburatani, Takao Hamakubo, Tatsuhiko Kodama, Makoto Naito,
Hepatocyte nuclear factor-4α (HNF4α; NR2A1) is an orphan member of the nuclear receptor superfamily involved in various processes that could influence endoderm development, glucose and lipid metabolism. A loss-of-function mutation in human HNF4α causes one form of diabetes mellitus called maturity-onset diabetes of the young type 1 (MODY1) which is characterized in part by a diminished insulin secretory response to glucose. The expression of HNF4α in a variety of tissues has been examined predominantly ...
Tópico(s): Diabetes Treatment and Management
2003 - BioMed Central | Nuclear Receptor
Yuichiro Watanabe, Toshiya Tanaka, Yasutoshi Uchiyama, Tetsu Takeno, Akashi Izumi, Hisahiko Yamashita, Junko Kumakura, Hiroko Iwanari, Shuying Jiang, Makoto Naito, David J. Mangelsdorf, Takao Hamakubo, Tatsuhiko Kodama,
Liver X activated receptor alpha (LXRalpha) forms a functional dimeric nuclear receptor with RXR that regulates the metabolism of several important lipids, including cholesterol and bile acids. As compared with RXR, the LXRalpha protein level in the cell is low and the LXRalpha protein itself is very hard to detect. We have previously reported that the mRNA for LXRalpha is highly expressed in human cultured macrophages. In order to confirm the presence of the LXRalpha protein in the human macrophage, ...
Tópico(s): interferon and immune responses
2003 - BioMed Central | Nuclear Receptor
Jean‐Sébastien Annicotte, Elisabeth Fayard, Galvin H. Swift, Lars Selander, Helena Edlund, Toshiya Tanaka, Tatsuhiko Kodama, Kristina Schoonjans, Johan Auwerx,
Liver receptor homolog 1 (LRH-1) and pancreatic-duodenal homeobox 1 (PDX-1) are coexpressed in the pancreas during mouse embryonic development. Analysis of the regulatory region of the human LRH-1 gene demonstrated the presence of three functional binding sites for PDX-1. Electrophoretic mobility shift assays and chromatin immunoprecipitation analysis showed that PDX-1 bound to the LRH-1 promoter, both in cultured cells in vitro and during pancreatic development in vivo. Retroviral expression of PDX- ...
Tópico(s): Genetics and Neurodevelopmental Disorders
2003 - Taylor & Francis | Molecular and Cellular Biology
Yasutomi Kamei, Hiroshi Ohizumi, Yasushi Fujitani, Tomoyuki Nemoto, Toshiya Tanaka, Nobuyuki Takahashi, Teruo Kawada, Masamitsu Miyoshi, Osamu Ezaki, Akira Kakizuka,
A well balanced body energy budget controlled by limitation of calorie uptake and/or increment of energy expenditure, which is typically achieved by proper physical exercise, is most effective against obesity and diabetes mellitus. Recently, peroxisome proliferator-activated receptor (PPAR) γ, a member of the nuclear receptor, and its cofactors have been shown to be involved in lipid metabolism and in the control of energy expenditure. Here we show that PPARγ coactivator 1 (PGC-1) β functions as ERRL1 ( ...
Tópico(s): Metabolism, Diabetes, and Cancer
2003 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Hiroto Ohguchi, Toshiya Tanaka, Aoi Uchida, Kenta Magoori, Hiromi Kudo, In‐Sook Kim, Kenji Daigo, Iori Sakakibara, Masashi Okamura, Hideo Harigae, Takeshi Sasaki, Timothy F. Osborne, Frank J. Gonzalez, Takao Hamakubo, Tatsuhiko Kodama, Juro Sakai,
Type 1 iodothyronine deiodinase (Dio1), a selenoenzyme catalyzing the bioactivation of thyroid hormone, is highly expressed in the liver. Dio1 mRNA and enzyme activity levels are markedly reduced in the livers of hepatocyte nuclear factor 4alpha (HNF4alpha)-null mice, thus accounting for its liver-specific expression. Consistent with this deficiency, serum T4 and rT3 concentrations are elevated in these mice compared with those in HNF4alpha-floxed control littermates; however, serum T3 levels are ...
Tópico(s): Genetic Syndromes and Imprinting
2008 - Taylor & Francis | Molecular and Cellular Biology
Mika Sugai, Hajime Umezu, Takashi Yamamoto, Shuying Jiang, Hiroko Iwanari, Toshiya Tanaka, Takao Hamakubo, Tatsuhiko Kodama, Makoto Naito,
Hepatocyte nuclear factor 4 alpha (HNF4 alpha) is a member of the nuclear receptor superfamily and is expressed in several endodermal tissues. The aim of the present study was to examine the expression of HNF4 alpha on ovarian epithelial tumors with immunocytochemistry and immunohistochemistry using mAbs recognizing P1 and P2 promoter-driven HNF4 alpha. Ovarian mucinous adenoma, mucinous tumors of borderline malignancy, and mucinous adenocarcinoma had positive nuclear staining for HNF4 alpha (41/45, ...
Tópico(s): Ovarian cancer diagnosis and treatment
2008 - Wiley | Pathology International
Keisuke Tachibana, Naohiko Anzai, Chihiro Ueda, Tatsuya Katayama, Daisuke Yamasaki, Takayoshi Kirino, Rika Takahashi, Kenji Ishimoto, Hidenori Komori, Toshiya Tanaka, Takao Hamakubo, Yukihiko Ueda, Hiroyuki Arai, Juro Sakai, Tatsuhiko Kodama, Takefumi Doi,
Although PDZK1 is a well‐known adaptor protein, the mechanisms for its role in transcriptional regulation are largely unknown. The peroxisome proliferator‐activated receptor alpha (PPARα) is a ligand‐activated transcription factor that plays an important role in the regulation of lipid homeostasis. Previously, we established a tetracycline‐regulated human cell line that can be induced to express PPARα and identified candidate target genes, one of which was PDZK1. In this study, we cloned and characterized ...
Tópico(s): Protein Kinase Regulation and GTPase Signaling
2008 - Wiley | FEBS Letters
Yukiyoshi Okauchi, Hiromi Iwahashi, Kohei Okita, Ming Yuan, Morihiro Matsuda, Toshiya Tanaka, Jun‐ichiro Miyagawa, Tohru Funahashi, Yukio Horikawa, Iichiro Shimomura, Kazuya Yamagata,
Peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1alpha) is a multifunctional transcriptional regulator for the pathways controlling mitochondrial biogenesis, oxidative metabolism, and glucose homeostasis. Genetic studies have suggested that Gly482Ser polymorphism of the PGC-1alpha gene is associated with a higher risk of type 2 diabetes, obesity, and hypertension. Adiponectin is an antidiabetic and antiatherogenic adipocytokine that is specifically produced by adipose tissue, ...
Tópico(s): Retinoids in leukemia and cellular processes
2008 - Japan Endocrine Society | Endocrine Journal
Kenji Daigo, Takeshi Kawamura, Yoshihiro Ohta, Riuko Ohashi, Satoshi Katayose, Toshiya Tanaka, Hiroyuki Aburatani, Makoto Naito, Tatsuhiko Kodama, Sigeo Ihara, Takao Hamakubo,
Hepatocyte nuclear factor-4α (HNF4α, NR2A1) is a nuclear receptor that has a critical role in hepatocyte differentiation and the maintenance of homeostasis in the adult liver. However, a detailed understanding of native HNF4α in the steady-state remains to be elucidated. Here we report the native HNF4α isoform, phosphorylation status, and complexes in the steady-state, as shown by shotgun proteomics in HepG2 hepatocarcinoma cells. Shotgun proteomic analysis revealed the complexity of native HNF4α, ...
Tópico(s): RNA modifications and cancer
2010 - Elsevier BV | Journal of Biological Chemistry