Ed. De Segundo, Ernest Newman, Frederick Grundy, S. H. Maxwell, Lloyd Georce, F. W. Wilson, A. Podmore, V. H. Goldsmith, E. N., Herbert D. Williams Editor of "Little Folks", Yarborough, T. P. O'connor M. P., Rt. Hon H. H. Asquith M. P., A. E. Hanford, Frank Rutter, J. Stewart Colonel I. A., Edmund Gosse, John Lane, Sydney W. Carroll, D. R. Gent, H. G. Wells, Roland Atkinson, Rt. Hon, L. Van Vliet, H. L. R., H. C. M., Emonn Dunne, S. C., Hannen Swaffer, Pandora, George Lambert M. P., Gerald Biss, S. M. E., M. J. G. Ritchi, R. J. Barrett Financial Editor, Harold Cox,
... by Mrs. F. Nevid Jackson. (The Bodley Head. Ú3 3s.) Sir Edward Thorpe's famous dictionary of… ...
1921 - Gale Group | Sunday Times HA GDA
Weiwei Dong, Yunhui Liu, Ping Wang, Xuelei Ruan, Libo Liu, Yixue Xue, Teng Ma, E Tiange, Di Wang, Chunqing Yang, Hongda Lin, Jian Song, Xiaobai Liu,
... investigate the role of C/D box snoRNA U3 and transcription factor zinc finger and BTB domain‐ ... western blot assays were utilized to detect snoRNA U3 and ZBTB7A expression. U3 promoter methylation status was analyzed via bisulfite sequencing ... the CpG island in the promoter region of U3 led to the upregulation of U3 expression in IDH1 WT GBM cells, and the knockdown of U3 suppressed aerobic glycolysis and the proliferation ability of ... We found that small nucleolar‐derived RNA (sdRNA) U3‐miR, a small fragment produced by U3, was ...
Tópico(s): MicroRNA in disease regulation
2023 - Wiley | CNS Neuroscience & Therapeutics
Mengge Du, Fan Liu, Yan Chang, Shuai Tong, Wei Liu, Yujiao Chen, Ping Xie,
... interacts with RRP9, a core component of the U3 snoRNP complex, which is involved in pre-rRNA ... interacts with RRP9, a core component of the U3 snoRNP complex, which is involved in pre-rRNA ... B. Donald H. Susan B. A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis.Nature. ... 970Google Scholar). SnoRNAs exist only in the nucleus; U3 is present in nucleoli and is associated with ... K. Steitz J.A. Sollner-Webb B. The U3 small nucleolar ribonucleoprotein functions in the first step ...
Tópico(s): Cancer-related gene regulation
2021 - Elsevier BV | Journal of Biological Chemistry
Nicolás Lemus-Diaz, Rafael Rinaldi Ferreira, Katherine E. Bohnsack, Jens Gruber, Markus T. Bohnsack,
... reveal that the atypical box C/D snoRNA U3, which functions as a scaffold during early ribosome ... stem-loop structure in the 5' domain of U3 is processed to form short RNA fragments that associate with Argonaute. miR-U3 production is independent of Drosha, and an increased amount of U3 in the cytoplasm in the absence of Dicer ... miRNAs. Using reporter assays, we demonstrate that miR-U3 can act as a low proficiency miRNA in ... the sortin nexin SNX27 mRNA as an endogenous U3-derived miRNA target. We further reveal that perturbation ...
Tópico(s): MicroRNA in disease regulation
2020 - Oxford University Press | Nucleic Acids Research
Guillaume Clerget, Valérie Bourguignon‐Igel, Nathalie Marmier‐Gourrier, Nicolas Rolland, Ludivine Wacheul, Xavier Manival, Christophe Charron, Joanna Kufel, Agnès Méreau, Véronique Senty-Segault, David Tollervey, Denis L. J. Lafontaine, Christiane Branlant, Mathieu Rederstorff,
U3 snoRNA and the associated Rrp9/U3-55K protein are essential for 18S rRNA production by the SSU-processome complex. U3 and Rrp9 are required for early pre-rRNA ... of the Rrp9 β-propeller structure opposite to U3 snoRNA. To understand this, we first characterized the ... showed strong synergistic negative interactions with mutations in U3 that destabilize the U3/pre-rRNA base-pair interactions or reduce the ... We propose that the Rrp9 β-propeller and U3/pre-rRNA binding cooperate in the structure or ... of the SSU-processome. Additionally, our analysis of U3 variants gave insights into the function of individual ...
Tópico(s): RNA and protein synthesis mechanisms
2020 - Oxford University Press | Nucleic Acids Research
Yuuri Hashimoto, Kumiko Koyama, Yasuki Kamai, Kenji Hirotani, Yusuke Ogitani, Akiko Zembutsu, Manabu Abe, Yuki Kaneda, Naoyuki Maeda, Yoshinobu Shiose, Takuma Iguchi, Tomomichi Ishizaka, Tsuyoshi Karibe, Ichiro Hayakawa, Koji Morita, Takashi Nakada, Taisei Nomura, Kenichi Wakita, Takashi Kagari, Yuki Abe, Masato Murakami, Suguru Ueno, Toshinori Agatsuma,
... therapy is currently clinically available. Here, we produced U3-1402, an anti-HER3 antibody–drug conjugate with ... pharmacologic activities and the mechanisms of action of U3-1402 were assessed in several human cancer cell lines. Antitumor activity of U3-1402 was evaluated in xenograft mouse models, including ... were also conducted in rats and monkeys. Results: U3-1402 showed HER3-specific binding followed by highly efficient cancer cell internalization. Subsequently, U3-1402 was translocated to the lysosome and released ...
Tópico(s): Toxin Mechanisms and Immunotoxins
2019 - American Association for Cancer Research | Clinical Cancer Research
Norikazu Masuda, Kan Yonemori, Shunji Takahashi, Takahiro Kogawa, Takahiro Nakayama, Hirotaka Iwase, Masato Takahashi, Tatsuya Toyama, Toshiaki Saeki, Shigehira Saji, Kenichi Inoue, Hiroshi Onuma, Naoyuki Tajima, Yoshihiko Shiose, S Chen, F. Guevara, Channing Yu, Shintaro Ueno, Hiroji Iwata,
... are no approved HER3-targeted anti-cancer therapies. U3-1402 is a HER3-targeted antibody drug conjugate ... to evaluate the safety, tolerability, and efficacy of U3-1402 in HER3-overexpressing metastatic breast cancer (MBC). ... Finding. Methods: In Dose Escalation, the dose of U3-1402 was escalated based on dose-limiting toxicity ... kg) identified during Dose Escalation. In both parts, U3-1402 was administered via IV infusion in 21- ... were to determine the safety and tolerability of U3-1402, the maximum tolerated dose (MTD), and the ...
Tópico(s): Cancer Treatment and Pharmacology
2019 - American Association for Cancer Research | Cancer Research
Mélanie Mahé, Florent Dufour, Hélène Neyret‐Kahn, Aura Moreno‐Vega, C Béraud, Mingjun Shi, Imène Hamaidi, Virginia Sánchez‐Quiles, Clémentine Krucker, Marion Dorland‐Galliot, Elodie Chapeaublanc, Rémy Nicolle, Hervé Lang, Célio Pouponnot, Thierry Massfelder, François Radvanyi, Isabelle Bernard‐Pierrot,
... in two bladder tumor-derived cell lines, MGH-U3 and RT112, harboring an FGFR3 mutation (Y375C) and ... FGFR3 in bladder carcinomas, by studying the MGH-U3 and RT112 cell lines. These cell lines were ... significant differential expression after FGFR3 depletion in MGH-U3 and RT112 cells, respectively (adjusted P-values < 0. ... observed after FGFR3 knockdown in RT112 and MGH-U3 cells (left panel). List of the top 10 ... and Methods). Relative MYC mRNA levels in MGH-U3 and RT112 cells transfected for 72 h with ... transfection) comparing FGFR3 and MYC levels in MGH-U3 and RT112 cells transfected with a control siRNA ( ...
Tópico(s): Bladder and Urothelial Cancer Treatments
2018 - Springer Nature | EMBO Molecular Medicine
Benjamin Rothé, Xavier Manival, Nicolas Rolland, Christophe Charron, Véronique Senty-Segault, Christiane Branlant, Bruno Charpentier,
The U3 box C/D snoRNA is one key element of 90S pre-ribosome. It contains a 5΄ domain ... the U3B/C and U3C΄/D motifs for U3 packaging into a unique small nucleolar ribonucleoprotein particle ( ... proteins Nop1p/FBL and Nop56p/NOP56, and the U3-specific protein Rrp9p/U3-55K. Snu13p/SNU13 has a much lower affinity ... assembly protein Rsa1 in the early steps of U3 snoRNP biogenesis in yeast and we propose a refined model of U3 snoRNP biogenesis. While recombinant Snu13p enhances the binding ... occurs by direct contacts of Rsa1p with the U3 snoRNA 5΄ domain. In light of the recent ...
Tópico(s): RNA and protein synthesis mechanisms
2017 - Oxford University Press | Nucleic Acids Research
Sifan Chen, Maximilian Felix Blank, Aishwarya Iyer, Bingding Huang, Lin Wang, Ingrid Grummt, Renate Voit,
... processing and rRNA maturation. Knockdown of SIRT7 impairs U3 snoRNA dependent early cleavage steps that are necessary for generation of 18S rRNA. Mechanistically, SIRT7 deacetylates U3-55k, a core component of the U3 snoRNP complex, and reversible acetylation of U3-55k modulates the association of U3-55k with U3 snoRNA. Deacetylation by SIRT7 enhances U3-55k binding to U3 snoRNA, which is a prerequisite for pre-rRNA ... is released from nucleoli, leading to hyperacetylation of U3-55k and attenuation of pre-rRNA processing. The ...
Tópico(s): Sirtuins and Resveratrol in Medicine
2016 - Nature Portfolio | Nature Communications
Richa Sardana, Xin Liu, Sander Granneman, Jieyi Zhu, Michael Gill, Ophelia Papoulas, Edward M. Marcotte, David Tollervey, Carl C. Correll, Alan M. Johnson,
In eukaryotes, the highly conserved U3 small nucleolar RNA (snoRNA) base-pairs to multiple sites in the pre-ribosomal RNA (pre-rRNA) to promote early cleavage and folding events. Binding of the U3 box A region to the pre-rRNA is ... box helicase Dhr1 (Ecm16) is responsible for displacing U3. An active site mutant of Dhr1 blocked release of U3 from the pre-ribosome, thereby trapping a pre- ... yet achieved its mature structure because it contained U3, pre-rRNA, and a number of early-acting ... in vivo to the pre-rRNA and to U3 sequences flanking regions that base-pair to the ...
Tópico(s): RNA Research and Splicing
2015 - Public Library of Science | PLoS Biology
Binal N. Shah, Xin Liu, Carl C. Correll,
Eukaryotic ribosome biogenesis requires rapid hybridization between the U3 snoRNA and the pre-rRNA to direct cleavages ... hybridization of one of the three duplexes that U3 makes with pre-rRNA, designated the U3-18S duplex, are buried in conserved structures: box A/A′ stem–loop in U3 snoRNA and helix 1 (H1) in the 18S ... Previously, we reported that Imp3 and Imp4 promote U3-18S hybridization in vitro, but the mechanism by which these proteins facilitate U3-18S duplex formation remained unclear. Here, we directly ...
Tópico(s): RNA and protein synthesis mechanisms
2013 - Cold Spring Harbor Laboratory Press | RNA
Patricia LoRusso, Pasi A. Jänne, Moacyr Oliveira, Naiyer A. Rizvi, Lisa Malburg, Vicki L. Keedy, Lorrin Yee, Catherine Copigneaux, Thore Hettmann, Chi‐Yuan Wu, Agnes Ang, Abdel‐Baset Halim, Robert A. Beckman, Darrin M. Beaupre, Jordan Berlin,
... poor prognosis. This first-in-human study of U3-1287 (NCT00730470), a fully human anti-HER3 monoclonal ... escalating doses (0.3–20 mg/kg) of U3-1287 every 2 weeks, starting 3 weeks after ... patients received 9, 14, or 20 mg/kg U3-1287 every 2 weeks, based on observed tolerability ... patients (part 1: 26; part 2: 31) received U3-1287. As no dose-limiting toxicities were reported, ... weeks. The most frequent adverse events related to U3-1287 were fatigue (21.1%), diarrhea (12.3%), ... and dysgeusia (5.3%). No patient developed anti-U3-1287 antibodies. In these heavily pretreated patients, stable ...
Tópico(s): Peptidase Inhibition and Analysis
2013 - American Association for Cancer Research | Clinical Cancer Research
... were detected in all regions, but only the U3 promoter region acquired statistically more mutations in the ... was determined for 12 candidate escape viruses with U3 mutations but no changes in the siRNA-target ... combinatorial attack, by acquiring mutations in the unrelated U3 promoter region. We, however, believe that there are ... too easily and have not demonstrated that the U3 mutations cause general siRNA escape. Several studies have ... mutations, was not used to scrutinize the indirect U3 region mutations. Supplementary Table S2 of Shah et ...
Tópico(s): Advanced biosensing and bioanalysis techniques
2012 - Cell Press | Molecular Therapy — Nucleic Acids
Daniel J. Freeman, Selam Ogbagabriel, James Bready, Ji-Rong Sun, Robert Radinsky, Thore Hettmann,
... report in vitro and in vivo activity of U3–1287 (AMG 888) as a single agent and ... SCCHN. Methods: FaDu (SCCHN) cells were treated with U3–1287 (AMG 888), panitumumab, the combination or control ... FaDu cells were treated with 1 μg/mL U3–1287 (AMG 888), 1 μg/ml panitumumab, the ... mm3 FaDu xenografts were treated 2×/week with U3–1287 (AMG 888), panitumumab, the combination or control mAbs. Results: Treatment with either U3–1287 (AMG 888) or panitumumab resulted in inhibition ... and pERK1/2. Although treatment with panitumumab or U3–1287 (AMG 888) alone significantly inhibited in vitro ...
Tópico(s): Radiopharmaceutical Chemistry and Applications
2011 - American Association for Cancer Research | Molecular Cancer Therapeutics
Bérengère Pradet‐Balade, Cyrille Girard, Séverine Boulon, Conception Paul, Karim Azzag, Rémy Bordonné, Édouard Bertrand, Céline Verheggen,
... In contrast, snoRNAs from independent gene, such as U3, U8 and U13, are synthesized from snRNA-type ... the nucleolus We previously showed that CRM1 binds U3 precursor RNAs, and by microinjection experiments, that it was required to transport U3 from CB to nucleoli (Boulon et al, 2004). ... B (LMB) led to the accumulation of microinjected U3 in CB and prevented its localization to nucleoli. ... wondered whether its role in the trafficking of U3 was dependent on functional nuclear pores or not. ... we microinjected HeLa cells with m7G-capped fluorescent U3 in the presence of wheat germ agglutinin (WGA), ...
Tópico(s): RNA regulation and disease
2011 - Springer Nature | The EMBO Journal
... of the six-torsion-angle sequences u1-u2-u3-u3′-u2′-u1′ of the 3,5-di(benzyloxy) ... synperiplanar). Of the 10 possible conformational types of u3-u3′ sequences, only six types were observed in single ... of 136 possible types of conformations of u2-u3-u3′-u2′ sequences, only 14. Ap-Sp and Ap-Ap are the preferred conformations of sequences u3-u3′, and Ap-Ap-Sp-Ap and Ap-Ap-Ap-Ap those of the sequences u2-u3-u3′-u2′. The presence of a substituent at position ...
Tópico(s): Advanced Polymer Synthesis and Characterization
2010 - American Chemical Society | Crystal Growth & Design
Rohit Aggarwal, Mary Lucas, Noreen Fertig, Chester V. Oddis, Thomas A. Medsger,
... demographic and clinical features, and survival in anti–U3 RNP autoantibody–positive patients with systemic sclerosis (SSc). Methods Medical records of 108 anti–U3 RNP–positive and 2,471 anti–U3 RNP–negative SSc patients first evaluated during 1985–2003 were reviewed. Anti–U3 RNP antibody was detected by protein and RNA ... analysis, and Mantel‐Haenszel test. Results The anti–U3 RNP–positive group had a higher proportion of ... 45% in those with and those without anti–U3 RNP, respectively). However, among patients with diffuse cutaneous ...
Tópico(s): Autoimmune Bullous Skin Diseases
2009 - Wiley | Arthritis & Rheumatism
Tı́mea Gérczei, Binal N. Shah, Anthony J. Manzo, Nils G. Walter, Carl C. Correll,
Short duplexes between the U3 small nucleolar RNA and the precursor ribosomal RNA must form quickly and with high yield to satisfy the high demand ... rapidly growing eukaryotic cells. These interactions, designated the U3–ETS (external transcribed spacer) and U3–18S duplexes, are essential to initiate the processing ... in vivo. Assembly of both proteins with the U3 small nucleolar RNA into a chaperone complex destabilizes a U3 stem structure, apparently to expose its 18S base- ... result, the chaperone complex accelerates formation of the U3–18S duplex from an undetectable rate to one ...
Tópico(s): RNA Research and Splicing
2009 - Elsevier BV | Journal of Molecular Biology
J. Michael Charette, Michael W. Gray,
U3 snoRNA is a box C/D small nucleolar RNA (snoRNA) involved in the processing events that ... from the ribosomal RNA precursor (pre-rRNA). Although U3 snoRNA is present in all eukaryotic organisms, most ... animals and plants. Relatively little is known about U3 snoRNA and its gene(s) in the phylogenetically ... previously revealed at least 13 bands hybridizing with U3 snoRNA, suggesting the existence of multiple copies of U3 snoRNA genes.Through screening of a lambda genomic library and PCR amplification, we recovered 14 U3 snoRNA gene variants, defined by sequence heterogeneities that ...
Tópico(s): RNA modifications and cancer
2009 - BioMed Central | BMC Genomics
Yumi Sasano, Yusuke Hokii, Kunio Inoue, Hiroshi Sakamoto, Chisato Ushida, Toshinobu Fujiwara,
U3 small nucleolar RNA (snoRNA) is one of the members of the box C/D class of ... to generate 18S rRNA in the nucleolus. Although U3 snoRNA is abundant, and is well conserved from yeast to mammals, the genes encoding U3 snoRNA in C. elegans have long remained unidentified. ... RNomics study in C. elegans predicted five distinct U3 snoRNA genes. However, characterization of these candidates for U3 snoRNA has yet to be performed. In this ... we isolated and characterized four candidate RNAs for U3 snoRNA from the immunoprecipitated RNAs of C. elegans ...
Tópico(s): CRISPR and Genetic Engineering
2008 - Elsevier BV | Biochimie
Zoltán Kerényi, Zsuzsanna Mérai, László Hiripi, Anna Hangyáné Benkovics, Péter Gyula, Christophe Lacomme, Endre Barta, Ferenc Nagy, Dániel Silhavy,
... P-silenced control (P) or U1-, U2- and U3-silenced test plants (U1, U2, U3) were infiltrated with G-L NMD test constructs ( ... the corresponding transcript levels of U1-, U2- and U3-silenced leaves are shown relative to it. At ... and Gc-I expressions are similarly increased in U3-silenced leaves relative to P-silenced control, whereas ... are referred to as P-, U1-, U2- and U3-silenced plants, respectively). In U1-silenced plants, both ... 3′UTR-based NMD activity of U1-, U2-, U3- and P-silenced plants was tested at 8 ...
Tópico(s): Viral Infections and Immunology Research
2008 - Springer Nature | The EMBO Journal
Hye Young Yi-Brunozzi, Stuart F.J. Le Grice,
... the ribonuclease H catalytic center at the PPT/U3 junction and also provides insights into nucleic acid ... the ribonuclease H catalytic center at the PPT/U3 junction and also provides insights into nucleic acid ... linkage; RNase H, ribonuclease H; RT, reverse transcriptase; U3, unique 3′ sequence; nt, nucleotide. -containing retrotransposons, second ... polypurine tract (PPT), and the 3′ unique sequence (U3) of the LTR; (b) DNA-dependent DNA synthesis ... a 15° bend at the HIV-1 PPT/U3 junction (7Fedoroff O.Y. Ge Y. Reid B. ...
Tópico(s): Cytomegalovirus and herpesvirus research
2005 - Elsevier BV | Journal of Biological Chemistry
Aiping Chen, Irene T. Weber, Robert W. Harrison, Jonathan Leis,
... IN) with DNA representing 20 bp of the U3 and U5 long terminal repeats (LTR) termini was ... IN) with DNA representing 20 bp of the U3 and U5 long terminal repeats (LTR) termini was ... using 15-bp substrates corresponding to the ASV U3 or U5 termini (9.Katzman M. Katz R. ... size, selected nucleotide substitutions in the U5 and U3 LTR regions were shown to affect one or ... specific nucleic acid positions in the HIV-1 U3 and U5 LTR regions can affect each of ... to the conserved CA dinucleotide in the MuLV U3 LTR region. When cells were infected with the ...
Tópico(s): RNA and protein synthesis mechanisms
2005 - Elsevier BV | Journal of Biological Chemistry
Chyan Leong Ng, David G. Waterman, Eugene V. Koonin, Alfred A. Antson, M. Ortiz-Lombardı́a,
... et al, 2004). Imp4 is part of the U3 small nucleolar ribonucleoprotein (U3 snoRNP; Lee & Baserga, 1999), a complex involved in ... the association of Mpp10 and Imp4 with the U3 snoRNA, suggesting that the RNA-binding domain of ... in vitro to a 5′ portion of the U3 snoRNA but not to the pre-rRNA (Gérczei & ... Imp4 is sufficient for its binding to the U3 snoRNA, but the full-length Imp4 is required ... the formation of two hybrid duplexes between the U3 snoRNA and the pre-rRNA (Gérczei & Correll, 2004). ... Dragon F et al (2002) A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis. Nature ...
Tópico(s): RNA Research and Splicing
2005 - Springer Nature | EMBO Reports
Ajaykumar C. Vora, Sibes Bera, Duane P. Grandgenett,
... I footprints were produced with wild type (wt) U3 and gain-of-function att site donors for ... C. Under suboptimal assembly conditions for intasomes using U3 att DNA, DNase I probing demonstrated an enhanced cleavage site 9 bp from the end of U3 suggesting that a transient structural intasome intermediate was ... a series of small size deletions of wt U3 att site sequences, we determined that sequences upstream ... I footprints were produced with wild type (wt) U3 and gain-of-function att site donors for ... C. Under suboptimal assembly conditions for intasomes using U3 att DNA, DNase I probing demonstrated an enhanced ...
Tópico(s): RNA Interference and Gene Delivery
2004 - Elsevier BV | Journal of Biological Chemistry
Anton V. Borovjagin, Susan A. Gerbi,
U3 small nucleolar RNA (snoRNA) is essential for rRNA processing to form 18S ribosomal RNA (rRNA). Previously, ... been shown that nucleolin is needed to load U3 snoRNA on pre-rRNA. However, as documented here, ... We present data that base-pairing between the U3 hinges and the external transcribed spacer (ETS) is critical for functional alignment of U3 on its pre-rRNA substrate. Additionally, the interaction between the U3 hinges and the ETS is proposed to serve as an anchor to hold U3 on the pre-rRNA substrate, while box A ...
Tópico(s): RNA Research and Splicing
2004 - Cold Spring Harbor Laboratory Press | RNA
Chandravanu Dash, Hye-Young Yi-Brunozzi, Stuart F.J. Le Grice,
... hybrids were evaluated for cleavage at the PPT/U3 junction. Altered specificity was observed when the homopolymeric ... 5′ of the PPT was modified, whereas PPT/U3 cleavage was lost after substitutions in the adjacent ... cleavage sites ahead (+5/+6) of the PPT/U3 junction. Differential cleavage at the PPT/U3 junction and U3 + 5/+6 site in response to LNA-induced ... hybrids were evaluated for cleavage at the PPT/U3 junction. Altered specificity was observed when the homopolymeric ... 5′ of the PPT was modified, whereas PPT/U3 cleavage was lost after substitutions in the adjacent ...
Tópico(s): Cytomegalovirus and herpesvirus research
2004 - Elsevier BV | Journal of Biological Chemistry
Daniela Lener, Mamuka Kvaratskhelia, Stuart F.J. Le Grice,
... terminal repeat-containing retrotransposons are accurately processed from (+) U3 RNA and DNA by their cognate reverse transcriptases ( ... the RNase H catalytic center over the PPT-U3 junction, a notion strengthened by additional mutagenesis studies ... terminal repeat-containing retrotransposons are accurately processed from (+) U3 RNA and DNA by their cognate reverse transcriptases ( ... the RNase H catalytic center over the PPT-U3 junction, a notion strengthened by additional mutagenesis studies ... RNA/DNA hybrid, precise hydrolysis at the PPT-U3 junction observed in vitro (9Rausch J.W. Le ...
Tópico(s): HIV/AIDS drug development and treatment
2003 - Elsevier BV | Journal of Biological Chemistry
Mature U3 snoRNA in yeast is generated from the 3'-extended precursors by endonucleolytic cleavage followed by exonucleolytic trimming. ... Lhp1p. We report that normal 3' processing of U3 requires the nuclear Lsm proteins. On depletion of ... Lsm8p, the normal 3'-extended precursors to the U3 snoRNA were lost. Truncated fragments of both mature and pre-U3 accumulated in the Lsm-depleted strains, consistent with substantial RNA degradation. Pre-U3 species were co-precipitated with TAP-tagged Lsm3p, but the association with spliced pre-U3 was lost in strains lacking Lhp1p. The association ...
Tópico(s): Plant Disease Resistance and Genetics
2003 - Oxford University Press | Nucleic Acids Research