Homayon Ghiasi, Anthony B. Nesburn, Steve Cai, Steven L. Wechsler,
... on sequence analysis, the protein encoded by the US5 open reading frame (ORF) of herpes simplex virus ... the designation of glycoprotein J (gJ). However, the US5 gene product has not been identified and the ... the DNA encoding the complete sequence of the US5 ORF, using a baculovirus expression system. Western blotting, using polyclonal antibody raised against synthetic US5 peptides, revealed two major baculovirus-US5-expressed protein bands with apparent molecular weights of 16–17 and 10 kD. The recombinant US5 was found on the membrane of <i>Spodoptera ...
Tópico(s): Cytomegalovirus and herpesvirus research
1998 - Karger Publishers | Intervirology
Kiersten L. Dionne, Danny Bergeron, Anne-Marie Landry-Voyer, François Bachand,
... the ribosome. For example, the 40S ribosomal protein uS5 (RPS2) is known to form an extraribosomal complex ... yeast to humans. However, the full scope of uS5's extraribosomal functions, including whether uS5 interacts with any other proteins, is not known. ... zinc finger protein 277 (ZNF277) as a new uS5-associated protein by using quantitative proteomics approaches in ... a C2H2-type zinc finger domain to recognize uS5. Analysis of protein–protein interactions in living cells indicated that the ZNF277–uS5 complex is found in the cytoplasm and the ...
Tópico(s): Peptidase Inhibition and Analysis
2018 - Elsevier BV | Journal of Biological Chemistry
Chuankuo Zhao, Tianqiong He, Yang Xu, Mingshu Wang, Anchun Cheng, Xinxin Zhao, Dekang Zhu, Shun Chen, Mafeng Liu, Qiao Yang, Renyong Jia, Xiaoyue Chen, Ying Wu, Shaqiu Zhang, Yunya Liu, Yanling Yu, Ling Zhang,
... molecular characterization and antiapoptotic function of the DPV Us5 gene. To perform molecular characterization of DPV Us5, RT-PCR and pharmacological inhibition tests were used to ascertain the kinetic class of the Us5 gene. Western blotting and an indirect immunofluorescence assay ( ... analyze the expression level and subcellular localization of Us5 in infected cells at different time points. Us5 in purified DPV virions was identified by mass ... Western blotting, and pharmacological inhibition tests revealed that Us5 is transcribed mainly in the late stage of ...
Tópico(s): Viral Infections and Immunology Research
2019 - Nature Portfolio | Scientific Reports
Anne-Marie Landry-Voyer, Zabih Mir Hassani, Mariano Avino, François Bachand,
... ribosomes in all living cells. The ribosomal protein uS5 (Rps2) is a stable component of the small ... proximal ribosomal proteins and rRNA inside the ribosome, uS5 has a surprisingly complex network of evolutionarily conserved ... we focus on a set of four conserved uS5-associated proteins: the protein arginine methyltransferase 3 (PRMT3), ... that presents PDCD2 and homologs as a dedicated uS5 chaperone and PDCD2L as a potential adaptor protein ... subunits. Although the functional significance of the PRMT3–uS5 and ZNF277–uS5 interactions remain elusive, we reflect ...
Tópico(s): Peptidase Inhibition and Analysis
2023 - Multidisciplinary Digital Publishing Institute | Biomolecules
Martine Aubert, Zheng Chen, Robin Lang, Chung H. Dang, Carla Fowler, Derek D. Sloan, Keith R. Jerome,
ABSTRACT The Us5 gene of herpes simplex virus (HSV) encodes glycoprotein J (gJ). The only previously reported function of gJ was ... evaluated the expression, localization, and cellular effects of Us5 /gJ. Us5 was first expressed 4 h after infection. gJ ... and was expressed in glycosylated and unglycosylated forms. Us5 was regulated as a late gene, with partial dependency on DNA replication for expression. Us5 expression was delayed in the absence of ICP22; furthermore, expression of Us5 in trans protected cells from apoptosis induced by ...
Tópico(s): Cell death mechanisms and regulation
2007 - American Society for Microbiology | Journal of Virology
Keith R. Jerome, Zheng Chen, Robin Lang, Monika R. Torres, Joni Hofmeister, Shannon L. Smith, Richard Fox, Christopher J. Froelich, Lawrence Corey,
... are involved in the inhibition of apoptosis, including Us5, which encodes glycoprotein J (gJ). Viruses deleted for Us5 showed defects in inhibition of caspase activation after ... ligation or UV irradiation. Transfected cells expressing the Us5 gene product gJ were protected from Fas- or ... by granzyme (gr)B was inhibited equivalently by Us5 deletion and rescue viruses, suggesting that gJ is ... However, mitochondria-free lysates from transfected cells expressing Us5/gJ were protected from grB-induced caspase activation, ...
Tópico(s): Hepatitis Viruses Studies and Epidemiology
2001 - American Association of Immunologists | The Journal of Immunology
Anne-Marie Landry-Voyer, Danny Bergeron, Carlo Yague‐Sanz, Breac Baker, François Bachand,
... PDCD2 specifically interacts with the 40S ribosomal protein uS5 (RPS2) and that the PDCD2-uS5 complex is assembled co-translationally. Loss of PDCD2 ... of the small ribosomal subunit that phenocopy a uS5 deficiency. Notably, we show that PDCD2 is important for the accumulation of soluble uS5 protein as well as its incorporation into 40S ... as a dedicated ribosomal protein chaperone that recognizes uS5 co-translationally in the cytoplasm and accompanies uS5 to ribosome assembly sites in the nucleus. As ...
Tópico(s): RNA Research and Splicing
2020 - Oxford University Press | Nucleic Acids Research
Lauren M. Hook, Finn Grey, Robert Grabski, Rebecca S. Tirabassi, Tracy J. Doyle, Meaghan H. Hancock, Igor Landais, Sophia Jeng, Shannon K. McWeeney, William J. Britt, Jay A. Nelson,
... unknown. We find that HCMV miRs UL112-1, US5-1, and US5-2 target multiple components of the host secretory ... SNAP23, and CDC42. A HCMV miR UL112-1, US5-1, and US5-2 triple mutant displayed aberrant morphogenesis of the ... infected cells. Ectopic expression of miRs UL112-1, US5-1, and US5-2 or siRNAs directed against RAB5C, RAB11A, SNAP23, ...
Tópico(s): Toxoplasma gondii Research Studies
2014 - Cell Press | Cell Host & Microbe
Meaghan H. Hancock, Lauren M. Hook, Jennifer Mitchell, Jay A. Nelson,
... study, we show that HCMV microRNAs (miRNAs) miR-US5-1 and miR-UL112-3p target the IκB ... α). Transfection of miR-UL112-3p and miR-US5-1 mimics reduced endogenous IKKα and IKKβ protein ... These phenotypes were rescued by preexpression of miR-US5-1 and miR-UL112-3p in infected cells or by a miR-US5-1/miR-UL112-3p double mutant virus that ... We show here that two HCMV miRNAs, miR-US5-1 and miR-UL112-3p, specifically downregulate IKKα ...
Tópico(s): Immune Response and Inflammation
2017 - American Society for Microbiology | mBio
Shujuan Jiang, Yujing Huang, Ying Qi, Rong He, Zhongyang Liu, Yanping Ma, Xin Guo, Yaozhong Shao, Zhengrong Sun, Qiang Ruan,
... to be a direct target of hcmv-miR-US5-1. Overexpression of hcmv-miR-US5-1 could block the accumulation of GMNN during ... decrease of GMNN expression caused by hcmv-miR-US5-1 or GMNN specific siRNA reduced HCMV DNA ... U373 cells. Meanwhile, ectopic expression of hcmv-miR-US5-1 and consequent lower expression of GMNN influenced ... and proliferation. These results imply that hcmv-miR-US5-1 may affect viral replication and host cellular ...
Tópico(s): RNA Interference and Gene Delivery
2017 - Elsevier BV | Virologica Sinica
Fernanda Romarı́s, María Auxiliadora Dea‐Ayuela, F. Bolás‐Fernández, A. R. Martínez‐Fernández, M. L. Sanmartín, Florencio M. Ubeira,
... isolates with the gp53‐specific monoclonal antibodies (mAbs) US5 and US9, produced in Btk xid mice, revealed ... kDa) and from T. nativa (55 kDa). mAb US5 reacted only with gp53 from T. spiralis. Experiments ... the presence of different potential inhibitors (recombinant gp53, US5, T. britovi‐ crude larval extract (CLE), and CLE ... different mouse strains. Whereas in BALB/c mice US5‐ and non‐US5‐recognized species‐specific epitopes on gp53 bind about ...
Tópico(s): Neurobiology and Insect Physiology Research
2003 - Wiley | Parasite Immunology
Martine Aubert, Elizabeth M. Krantz, Keith R. Jerome,
... one of the two antiapoptotic genes Us3 and Us5. To evaluate CTL-mediated apoptosis, we used a ... as well as wild-type viruses. In contrast, Us5-deleted virus showed defects in the ability to ... CTL attack occurred later. In contrast, Us3- or Us5-deleted virus showed no measurable defect in their ... infected cells under CTL attack. Furthermore, the Us3, Us5, and Us12 viral genes each have unique inhibitory ...
Tópico(s): interferon and immune responses
2006 - Mary Ann Liebert, Inc. | Viral Immunology
Meaghan H. Hancock, Jennifer Mitchell, Felicia Goodrum, Jay A. Nelson,
... study, we found that the HCMV miRNA miR-US5-2 targets the epidermal growth factor receptor (EGFR) ... cellular signaling pathways activated by EGF. Consequently, miR-US5-2 blocks the EGF-mediated proliferation of human ... expression of HCMV UL138. We show that miR-US5-2 regulates UL138 expression through GAB1-mediated downregulation ... to EGR1 expression. These data suggest that miR-US5-2, through downregulation of GAB1, could play a ...
Tópico(s): Cancer-related molecular mechanisms research
2020 - American Society for Microbiology | mSphere
María Jesús Perteguer, Eloísa Ramos‐Rodríguez, Fernanda Romarı́s, Marcela Escalante, Pedro Bonay, Florencio M. Ubeira, M GARATE,
... US9), or gp53 from Trichinella spiralis alone (mAb US5). Based on previously published sequences of this glycoprotein [ ... residue changes) between the two species. Mapping of US5- and US9-recognized epitopes was undertaken through the ... region, while the corresponding epitope recognized by mAb US5 was a 47-amino acid sequence containing two ... Trichinella pseudospiralis gp53). Additionally, the stability of the US5 epitope seems to depend on correct folding of ...
Tópico(s): Glycosylation and Glycoproteins Research
2004 - Elsevier BV | Molecular Immunology
Nobuyoshi Jinnai, Takuro Sakagami, Takashi Sekigawa, Miho Kakihara, Toshiaki Nakajima, Kenichi Yoshida, Shin Goto, Takashi Hasegawa, Takeshi Koshino, Yoshinori Hasegawa, Hiromasa Inoue, Naohito Suzuki, Yasuyuki Sano, Ituro Inoue,
... in the promoter region of the EP2 gene, uS5, uS5b, and uS7, were significantly associated with AIA ( ... P=0.001). The most significantly associated SNP, uS5, located in the regulatory region of the EP2 ... assay in HCT116 cells, the site containing the uS5 allele showed reduced transcription activity. Taken together, these results suggest that uS5 allele serves as a target of a transcription ...
Tópico(s): Urticaria and Related Conditions
2004 - Oxford University Press | Human Molecular Genetics
Divya Kamath, Steven T. Gregory, Michael B. O’Connor,
Ribosomal protein uS5 is an essential component of the small ribosomal subunit that is involved in subunit assembly, maintenance of translational ... the antibiotic spectinomycin. While many of the characterized uS5 mutations that affect decoding map to its interface ... has shown that residues distant from the uS4-uS5 interface can also affect the decoding process. We ...
Tópico(s): Bacterial Genetics and Biotechnology
2016 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
Meaghan H. Hancock, Lindsey B. Crawford, Wilma Perez, Hillary M. Struthers, Jennifer Mitchell, Patrizia Caposio,
... that signals through the FLT3 receptor, and miR-US5-1 and miR-UL112-3p to reduce the ... CD34+ HPCs. Regulation of FOXO3a by pUL7, miR-US5-1, and miR-UL112 results in reduced expression ... of CD34+ HPCs. HCMV pUL7 and miRNAs miR-US5-1 and miR-UL112-3p act to limit ...
Tópico(s): Immune Cell Function and Interaction
2021 - American Society for Microbiology | mSphere
Danielle M. Garshott, Heeseon An, Elayanambi Sundaramoorthy, Marilyn Leonard, Alison C. Vicary, J. Wade Harper, Eric J. Bennett,
... ubiquitylation events on the 40S proteins uS3 and uS5 are induced by diverse proteostasis stressors that impact ... as the key enzymes that regulate uS3 and uS5 ubiquitylation. Prolonged uS3 and uS5 ubiquitylation results in 40S, but not 60S, ribosomal ... triggers site-specific ubiquitylation events on ribosomal proteins uS5 and uS3. This study identifies and characterizes a ...
Tópico(s): Peptidase Inhibition and Analysis
2021 - Cell Press | Cell Reports
Keizo Kohno, Takeshi Yamamoto, Atsushi Kuroiwa, Kazunobu Amako,
The pili of Serratia marcescens US5 isolated from a patient with urinary tract infection were purified and characterized. During the aeration culture, the pili were detached from ... the outer membrane proteins extracted from S. marcescens US5 in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These ...
Tópico(s): Genomics and Phylogenetic Studies
1984 - American Society for Microbiology | Infection and Immunity
Takeshi Yamamoto, Asami Ariyoshi, Kazunobu Amako,
... Access Fimbria-Mediated Adherence of Serratia marcescens Strain US5 to Human Urinary Bladder Surface Takeshi Yamamoto, Takeshi ... K. 1984. Purification and characterization of Serratia marcescens US5 pili. Infect. Immun. 46: 295– 300. 8Lancaster, L. ...
Tópico(s): Pelvic floor disorders treatments
1985 - Wiley | Microbiology and Immunology
... region there are four genes, designated US2, US4, US5 and US7, whose functions are unknown but whose ... was found that the products of genes US4, US5 and US7 were convincingly classified as membrane-translated, ... a less definitive result. In conclusion, the US4, US5 and US7 gene products were considered probably to ...
Tópico(s): Fractal and DNA sequence analysis
1985 - Elsevier BV | Virus Research
Prakash Balan, Nicholas Davis‐Poynter, Susanne Bell, Helen Atkinson, Helena Browne, Tony Minson,
... expression cassette within the US4, US8, US7 and US5 genes respectively. Revertant viruses were then constructed by ... viruses showed the following, (i) Interruption of the US5 (gJ) gene has no effect on the ability ... central nervous system (CNS). This shows that the US5 gene provides a convenient site for the insertion ...
Tópico(s): Cytomegalovirus and herpesvirus research
1994 - Microbiology Society | Journal of General Virology
Uladzimir Karniychuk, Marc Geldhof, Merijn Vanhee, Jan Van Doorsselaere, Tamara A Saveleva, Hans Nauwynck,
... 1 strain Lelystad and the North American strain US5, sets of monospecific polyclonal antisera were tested in ... points. For antisera against PRRSV (Lelystad) and PRRSV (US5), heterologous antibody titres were significantly lower than homologous ... from European subtype 1 Lelystad and North American US5 strains at both the genetic and antigenic level.
Tópico(s): Virus-based gene therapy research
2010 - BioMed Central | BMC Veterinary Research
Rahul Rathakrishnan, Aravinda K. Therimadasamy, Y.H. Chan, Einar Wilder‐Smith,
... the ulnar sensory nerve conduction to digit 5 (US5) as comparator nerves for diagnosing CTS. In a ... subjects, we determined and compared the PCBm and US5 conduction velocity. We examined 57 hands with clinically ... PCBm conduction (p < 0.0001) but not for US5 conduction (p = 0.488). Using a 3 percentile ...
Tópico(s): Nerve Injury and Rehabilitation
2007 - Elsevier BV | Clinical Neurophysiology
Prem Bajaj, Thomas Graven‐Nielsen, Anthony Wright, lolo ab Ithel Davies, Lars Arendt‐Nielsen,
... a 2 Hz train of five ultrasonic stimuli (US5) were used to assess the FDI. Measurements were ... any time before or after the exercise, whereas US5 was significantly lower than control after 24 hours ( ... threshold, calculated as a ratio between US1 and US5, was maximum at 24 hours compared with the ...
Tópico(s): Exercise and Physiological Responses
2000 - Elsevier BV | Journal of Pain
María Auxiliadora Dea‐Ayuela, Florencio M. Ubeira, Aída Pitarch, Concha Gil, A. R. Martínez‐Fernández, F. Bolás‐Fernández,
The antigens recognised by mAb US5 specific to 53 kDa glycoprotein (gp 53) in T. spiralis L-1 muscle larvae (TSL1) antigens, mAb US9 specific to gp ... proteins of different pI were recognised by mAb US5 on T. spiralis antigens. Recognition profile of mAb ... some variation with regard to that of the US5. Polymorphism was apparent in gp 53. High reactivity ...
Tópico(s): Insect and Pesticide Research
2001 - EDP Sciences | Parasite
Produção Nacional
Revisado por pares
Huikyo Lee, Gülçin Yıldız, Luana Cristina dos Santos, Shaotong Jiang, Juan E. Andrade, Nicki J. Engeseth, Hao Feng,
... 12 followed by sonication for 5 min (pH12 + US5) was the most effective in reducing the sizes ... surface hydrophobicity, and modifying protein subunits. The pH12 + US5 treated SPI increased protein solubility from 1.49% ... of 22 nm. SPI nanoemulsions prepared using pH12 + US5 treated protein and canola oil had particle sizes ...
Tópico(s): Food composition and properties
2015 - Elsevier BV | Food Hydrocolloids
... since April 2015. The items necessary for scoring US5 scores (the ‘hand-limited version’ of the German ... EULAR-OMERACT combined scoring system. In RA patients, US5 scores were determined for the involved hands. Results: ... 27 hands with paratenonitis of 25 RA patients, US5 scores were compared based on the absence or ...
Tópico(s): Orthopedic Surgery and Rehabilitation
2020 - BMJ | Annals of the Rheumatic Diseases
Vladimir Jovasevic, Bernard Roizman,
... RNAs. We describe here a novel RNA designated US5-1 that spans 4.5 kb of the unique-short (US) region. The RNA initiates in US5 and terminates in the α22 open reading frame. It is expressed antisense to US5, US4, US3 and ICP22 mRNAs. This transcript is ...
Tópico(s): Animal Disease Management and Epidemiology
2010 - BioMed Central | Virology Journal
Thomas R. Jones, V P Muzithras,
... were isolated. A recombinant lacking IRS1, US1 through US5, and most of the US6 family was also ...
Tópico(s): RNA regulation and disease
1992 - American Society for Microbiology | Journal of Virology