Yoshiyuki Akiyama, Jimpei Miyakawa, Michael A. O’Donnell, Karl J. Kreder, Yi Luo, Daichi Maeda, Tetsuo Ushiku, Haruki Kume, Yukio Homma,
... Karl J. Kreder, Yi Luo, Daichi Maeda, Tetsuo Ushiku, Haruki Kume, and Yukio Homma Yoshiyuki AkiyamaYoshiyuki Akiyama * ... Japan More articles by this author , Tetsuo UshikuTetsuo Ushiku Department of Pathology, Graduate School of Medicine, The ... Kanazawa, Japan More articles by this author Tetsuo Ushiku Department of Pathology, Graduate School of Medicine, The ...
Tópico(s): Urinary and Genital Oncology Studies
2021 - Lippincott Williams & Wilkins | The Journal of Urology
Maho Oishi, Yukiko Inoue, R. Kagatsume, Takehito Shukuya, R. Kasukabe, Hiroshi Oya, Shigeyuki HOSHINO, S. Ushiku, Y. Fujiwara, Y. Motokura, Yoshiaki Maeda,
... Shukuya, R. Kasukabe, H. Oya, S. Hoshino, S. Ushiku, Y. Fujiwara, Y. Motokura, and Y. MaedaM. Oishi† ... Shukuya, R. Kasukabe, H. Oya, S. Hoshino, S. Ushiku, Y. Fujiwara, Y. Motokura, and Y. MaedaAffiliationsAuthors and ... Shukuya R. Kasukabe H. Oya S. Hoshino S. Ushiku Y. Fujiwara Y. Motokura , Yokohama Plant Protection Station, ...
Tópico(s): Herpesvirus Infections and Treatments
2017 - American Phytopathological Society | Plant Disease
Hidenori Kage, Aya Shinozaki‐Ushiku, Kazunaga Ishigaki, Yusuke Sato, Masahiko Tanabe, Shota Tanaka, Michihiro Tanikawa, Kousuke Watanabe, Shingo Kato, Kiwamu Akagi, Keita Uchino, Kinuko Mitani, Shunji Takahashi, Yuji Miura, Sadakatsu Ikeda, Yasushi Kojima, Kiyotaka Watanabe, Hitoshi Mochizuki, Hironori Yamaguchi, Yoshimasa Kawazoe, Kosuke Kashiwabara, Shinji Kohsaka, Kenji Tatsuno, Tetsuo Ushiku, Kazuhiko Ohe, Yutaka Yatomi, Yasuyuki Seto, Hiroyuki Aburatani, Hiroyuki Mano, Kiyoshi Miyagawa, Katsutoshi Oda,
Comprehensive cancer genome profiling (CGP) has been nationally reimbursed in Japan since June 2019. Less than 10% of the patients have been reported to undergo recommended treatment. Todai OncoPanel (TOP) is a dual DNA-RNA panel as well as a paired tumor-normal matched test. Two hundred patients underwent TOP as part of Advanced Medical Care B with approval from the Ministry of Health, Labour and Welfare between September 2018 and December 2019. Tests were carried out in patients with cancers without ...
Tópico(s): Genetic factors in colorectal cancer
2023 - Wiley | Cancer Science
Rie Tanaka, Toshikatsu Kaburaki, Kazuki Taoka, Ayako Karakawa, Hideki Tsuji, Masako Nishikawa, Yutaka Yatomi, Aya Shinozaki‐Ushiku, Tetsuo Ushiku, Fumiyuki Araki,
Purpose To establish diagnostic criteria for vitreoretinal lymphoma (VRL) using cytology and laboratory tests from vitreous samples: interleukin (IL)-10/IL-6 ratio, immunoglobulin (Ig) H gene rearrangement, and clonal B-cells on flow cytometry.Methods Fifty-six patients with and 39 without VRL were included. We assessed the sensitivity and specificity of each test and those of diagnostic criteria based on combinations of these tests.Results The sensitivity values for malignant cytology, IL-10/IL-6 > ...
Tópico(s): Glioma Diagnosis and Treatment
2021 - Taylor & Francis | Ocular Immunology and Inflammation
Paul Cray, Breanna Sheahan, Christopher M. Dekaney,
... Middelhoff M. Hikiba Y. Higashijima N. Ihara S. Ushiku T. Fukayama M. Tailor Y. Hirata Y. Guha ... Middelhoff M. Hikiba Y. Higashijima N. Ihara S. Ushiku T. Fukayama M. Tailor Y. Hirata Y. Guha ...
Tópico(s): Infant Nutrition and Health
2021 - Elsevier BV | Cellular and Molecular Gastroenterology and Hepatology
Sultan Ahmad Halimi, Daichi Maeda, Aya Shinozaki‐Ushiku, Akiteru Goto, Katsutoshi Oda, Yutaka Osuga, Tomoyuki Fujii, Tetsuo Ushiku, Masashi Fukayama,
Mucinous cystadenoma is one of the most common benign ovarian neoplasms. The immunophenotypes and histogenetic relationships of mucinous cystadenomas with a Müllerian-type epithelium have not been fully explored. We elucidated the direction of differentiation of the mucinous epithelium that constitutes mucinous cystadenomas. Special attention was paid to the existence of gastrointestinal (GI)-type mucinous epithelium, and its association with background Müllerian-type epithelium. Immunohistochemistry ...
Tópico(s): Gynecological conditions and treatments
2020 - Elsevier BV | Human Pathology
Aya Shinozaki‐Ushiku, Shumpei Ishikawa, Daisuke Komura, Yasuyuki Seto, Hiroyuki Aburatani, Tetsuo Ushiku,
Tópico(s): Lung Cancer Treatments and Mutations
2020 - Springer Science+Business Media | Gastric Cancer
Tetsuo Ushiku, Akiko Kunita, Ryohei Kuroda, Aya Shinozaki‐Ushiku, Sho Yamazawa, Yosuke Tsuji, Mitsuhiro Fujishiro, Masashi Fukayama,
Gastric neoplasms exhibiting oxyntic gland differentiation typically are composed of cells with mild cytonuclear atypia differentiating to chief cells and to a lesser extent, parietal cells. Such tumors with atypical features have been reported also and terminology for this entity remains a matter of considerable debate. We analyzed and classified 26 tumors as oxyntic gland neoplasms within mucosa (group A, eight tumors) and with submucosal invasion. The latter was divided further into those with ...
Tópico(s): Gastric Cancer Management and Outcomes
2019 - Elsevier BV | Modern Pathology
Masashi Fukayama, Hiroyuki Abé, Akiko Kunita, Aya Shinozaki‐Ushiku, Keisuke Matsusaka, Tetsuo Ushiku, Atsushi Kaneda,
Tópico(s): Gastrointestinal disorders and treatments
2019 - Springer Science+Business Media | Virchows Archiv
Hidenori Kage, Shinji Kohsaka, Aya Shinozaki‐Ushiku, Yoshihisa Hiraishi, Jiro Sato, Kazuhiro Nagayama, Tetsuo Ushiku, Daiya Takai, Jun Nakajima, Kiyoshi Miyagawa, Hiroyuki Aburatani, Hiroyuki Mano, Takahide Nagase,
Abstract Next‐generation sequencing ( NGS ) has been implemented in clinical oncology to analyze multiple genes and to guide therapy. In patients with advanced lung cancer, small biopsies such as computed tomography‐guided needle biopsy ( CTNB ), endobronchial ultrasound‐guided transbronchial needle aspiration ( EBUS ‐ TBNA ) and transbronchial biopsy ( TBB ) are less invasive and are preferable to resection to make a pathological diagnosis. However, the quality of DNA / RNA and NGS from small lung tumor biopsy samples ...
Tópico(s): Genomics and Phylogenetic Studies
2019 - Wiley | Cancer Science
Man Wang, Bianli Gu, Xinzhe Chen, Yefu Wang, Peifeng Li, Kun Wang,
... lines was further profiled by deep sequencing.63Shinozaki-Ushiku A. Kunita A. Isogai M. Hibiya T. Ushiku T. Takada K. Fukayama M. Profiling of Virus- ...
Tópico(s): Circular RNAs in diseases
2019 - Cell Press | Molecular Therapy — Nucleic Acids
Shinji Kohsaka, Kenji Tatsuno, Toshihide Ueno, Masaaki Nagano, Aya Shinozaki‐Ushiku, Tetsuo Ushiku, Daiya Takai, Masachika Ikegami, Hiroshi Kobayashi, Hidenori Kage, Mizuo Ando, Keisuke Hata, Hiroki Ueda, Shogo Yamamoto, Shinya Kojima, Kumiko Oseto, Keisuke Akaike, Yoshiyuki Suehara, Takuo Hayashi, Tsuyoshi Saito, Fumiyuki Takahashi, Kazuhisa Takahashi, Kazuya Takamochi, Kenji Suzuki, Satoshi Nagayama, Yoshinao Oda, Koshi Mimori, Soichiro Ishihara, Yutaka Yatomi, Takahide Nagase, Jun Nakajima, Sakae Tanaka, Masashi Fukayama, Katsutoshi Oda, Masaomi Nangaku, Kohei Miyazono, Kiyoshi Miyagawa, Hiroyuki Aburatani, Hiroyuki Mano,
Tumor molecular profiling is becoming a standard of care for patients with cancer, but the optimal platform for cancer sequencing remains undetermined. We established a comprehensive assay, the Todai OncoPanel ( TOP ), which consists of DNA and RNA hybridization capture‐based next‐generation sequencing panels. A novel method for target enrichment, named the junction capture method, was developed for the RNA panel to accurately and cost‐effectively detect 365 fusion genes as well as aberrantly spliced ...
Tópico(s): Advanced biosensing and bioanalysis techniques
2019 - Wiley | Cancer Science
Masayuki Urabe, Tetsuo Ushiku, Aya Shinozaki‐Ushiku, Akiko Iwasaki, Sho Yamazawa, Hiroharu Yamashita, Yasuyuki Seto, Masashi Fukayama,
Abstract Adenocarcinoma of the esophagogastric junction (AEG) has heterogeneous carcinogenic process due to its location straddling the esophagogastric junction. We assessed background mucosal pathology and its correlation with clinicopathological features of each Siewert type of AEG. Clinicopathological and immunohistochemical analyses of 103 AEGs and 58 gastric cancers (GCs) were conducted. Background mucosal features were evaluated according to the updated Sydney System. Siewert classification ...
Tópico(s): Gastrointestinal Tumor Research and Treatment
2018 - Wiley | Cancer Medicine
Hiroyuki Abé, Ruri Saito, Takashi Ichimura, Akiko Iwasaki, Sho Yamazawa, Aya Shinozaki‐Ushiku, Teppei Morikawa, Tetsuo Ushiku, Hiroharu Yamashita, Yasuyuki Seto, Masashi Fukayama,
Tópico(s): Cancer Immunotherapy and Biomarkers
2018 - Springer Science+Business Media | Virchows Archiv
Sho Yamazawa, Tetsuo Ushiku, Aya Shinozaki‐Ushiku, Akimasa Hayashi, Akiko Iwasaki, Hiroyuki Abé, Amane Tagashira, Hiroharu Yamashita, Yasuyuki Seto, Hiroyuki Aburatani, Masashi Fukayama,
A primitive cell-like gene expression signature is associated with aggressive phenotypes of various cancers. We assessed the expression of phenotypic markers characterizing primitive cells and its correlation with clinicopathologic and molecular characteristics in gastric cancer. Immunohistochemical analysis of a panel of primitive phenotypic markers, including embryonic stem cell markers (OCT4, NANOG, SALL4, CLDN6, and LIN28) and known oncofetal proteins (AFP and GPC3), was performed using tissue ...
Tópico(s): Cancer Genomics and Diagnostics
2017 - Lippincott Williams & Wilkins | The American Journal of Surgical Pathology
Aya Shinozaki‐Ushiku, Tetsuo Ushiku, Shigeki Morita, Masaki Anraku, Jun Nakajima, Masashi Fukayama,
Aims Malignant mesothelioma is a highly aggressive cancer that is usually diagnosed at advanced stages; thus, highly sensitive and specific markers are necessary for its early definitive diagnosis. The aim of this study was to evaluate the diagnostic utility and prognostic significance of BAP 1 and EZH 2 in malignant mesothelioma. Methods and results The expression of BAP 1 and EZH 2 was investigated by immunohistochemistry in 32 malignant mesotheliomas and 44 benign mesothelial proliferative lesions, ...
Tópico(s): Renal and related cancers
2016 - Wiley | Histopathology
Aya Shinozaki‐Ushiku, Akiko Kunita, Maya Isogai, Takashi Hibiya, Tetsuo Ushiku, Kenzo Takada, Masashi Fukayama,
ABSTRACT Epstein-Barr virus (EBV) is one of the major oncogenic viruses and is found in nearly 10% of gastric carcinomas. EBV is known to encode its own microRNAs (miRNAs); however, their roles have not been fully investigated. The present report is the largest series to comprehensively profile the expression of 44 known EBV miRNAs in tissue samples from patients with EBV-associated gastric carcinoma. Several miRNAs were highly expressed in EBV-associated gastric carcinoma, and in silico analysis ...
Tópico(s): RNA modifications and cancer
2015 - American Society for Microbiology | Journal of Virology
... has been confusion concerning the ethical procedures at Ushiku Aiwa General Hospital where I did clinical research. ... School of Medicine, I was unfairly blamed for Ushiku’s informal procedures. As a result of a ...
Tópico(s): Cardiac, Anesthesia and Surgical Outcomes
2012 - Wiley | Anaesthesia
Tetsuo Ushiku, Aya Shinozaki‐Ushiku, Daichi Maeda, Shigeki Morita, Masashi Fukayama,
This study aimed to clarify claudin-6 expression patterns in cancers.We surveyed claudin-6 expression by immunohistochemistry using tissue microarray in 860 tumours, including germ cell tumours and major carcinomas. Claudin-6 was expressed consistently in germ cell tumours (28 of 28, 100%), whereas only 64 (8%) of 832 non-germ cell tumours demonstrated claudin-6 expression. Further immunohistochemical study in full tissue sections demonstrated diffuse claudin-6 staining in all seminomas (n=14), embryonal ...
Tópico(s): Epigenetics and DNA Methylation
2012 - Wiley | Histopathology
Minhua Sun, Hiroshi Uozaki, Rumi Hino, Akiko Kunita, Aya Shinozaki‐Ushiku, Tetsuo Ushiku, Takashi Hibiya, Kimiko Takeshita, Maya Isogai, Kenzo Takada, Masashi Fukayama,
Tópico(s): Gender Studies in Language
2012 - Springer Science+Business Media | Virchows Archiv
Hiroyuki Abé, Daichi Maeda, Rumi Hino, Yuya Otake, Maya Isogai, Aya Ushiku, Keisuke Matsusaka, Akiko Kunita, Tetsuo Ushiku, Hiroshi Uozaki, Yoko Tateishi, Tsunekazu Hishima, Yoshiaki Iwasaki, Shumpei Ishikawa, Masashi Fukayama,
Tópico(s): Cancer-related gene regulation
2012 - Springer Science+Business Media | Virchows Archiv
Teppei Morikawa, Rumi Hino, Hiroshi Uozaki, Daichi Maeda, Tetsuo Ushiku, Aya Shinozaki‐Ushiku, Takashi Sakatani, Masashi Fukayama,
Ribonucleotide reductase M2 subunit is one of two subunits that constitute ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleotide 5′-diphosphates into 2′-deoxyribonucleotides, which are required for DNA synthesis. This study was conducted to investigate the roles of ribonucleotide reductase M2 subunit in gastric cancer. The expression of ribonucleotide reductase M2 subunit protein was examined by immunohistochemistry. In normal gastric mucosa, ribonucleotide reductase ...
Tópico(s): Viral-associated cancers and disorders
2010 - Elsevier BV | Human Pathology
Tetsuo Ushiku, Aya Shinozaki‐Ushiku, Hiroshi Uozaki, Yoshiaki Iwasaki, Yoko Tateishi, Nobuaki Funata, Yasuyuki Seto, Masashi Fukayama,
Osteoclast-like giant cells (OGC) are rare in gastric carcinomas. Histopathological study of seven gastric carcinomas with OGC demonstrated three distinct types: lymphoepithelioma-like carcinoma (LELC), non-LELC, and giant cell tumor (GCT) types. LELC is a poorly differentiated adenocarcinoma with prominent lymphoid stroma. The LELC type (n = 4) showed similar histology to LELC of the stomach, except that they were accompanied by OGC and granulomatous reaction. Epstein-Barr virus (EBV) infection was demonstrated ...
Tópico(s): Cholangiocarcinoma and Gallbladder Cancer Studies
2010 - Wiley | Pathology International
Tetsuo Ushiku, Aya Shinozaki‐Ushiku, Junji Shibahara, Yoshiaki Iwasaki, Yoko Tateishi, Nobuaki Funata, Masashi Fukayama,
The novel stem cell marker SALL4 has been identified as a diagnostic marker of germ cell tumors, especially yolk sac tumors, in gonadal organs. To clarify the significance of SALL4 as an oncofetal protein, we investigated SALL4 expression by immunohistochemistry in non-neoplastic stomach and gastric carcinoma with particular emphasis on á-fetoprotein (AFP)-producing gastric carcinoma, as AFP-producing gastric carcinoma shares expression of AFP and glypican 3 (GPC3) with yolk sac tumors and hepatic ...
Tópico(s): Tumors and Oncological Cases
2010 - Lippincott Williams & Wilkins | The American Journal of Surgical Pathology
Aya Shinozaki‐Ushiku, Takashi Sakatani, Tetsuo Ushiku, Rumi Hino, Maya Isogai, Shunpei Ishikawa, Hiroshi Uozaki, Kenzo Takada, Masashi Fukayama,
Abstract EBV-associated gastric carcinoma is a distinct gastric carcinoma subtype with characteristic morphologic features similar to those of cells that undergo epithelial-to-mesenchymal transition. The effect of microRNA abnormalities in carcinogenesis was investigated by measuring the expression of the epithelial-to-mesenchymal transition–related microRNAs, miR-200a and miR-200b, in 36 surgically resected gastric carcinomas using quantitative reverse transcription-PCR analysis. MiR-200 family ...
Tópico(s): Circular RNAs in diseases
2010 - American Association for Cancer Research | Cancer Research
Aya Shinozaki‐Ushiku, Tetsuo Ushiku, Teppei Morikawa, Rumi Hino, Takashi Sakatani, Hiroshi Uozaki, Masashi Fukayama,
Epstein-Barr virus (EBV)-associated gastric carcinoma (GC) is a distinct subtype with characteristic clinicopathological features. To better characterize its cellular characteristics, 43 cases of EBV-associated GC, 68 cases of EBV-negative GC, and non-neoplastic gastric mucosa in adults and fetuses were examined immunohistochemically. We quantified the expression of the major tight-junction protein claudin (CLDN) -1, -3, -4, -7, and -18 together with gastric mucins (MUC5AC and MUC6), intestinal mucin (MUC2), ...
Tópico(s): Helicobacter pylori-related gastroenterology studies
2009 - SAGE Publishing | Journal of Histochemistry & Cytochemistry
Aya Shinozaki‐Ushiku, Tetsuo Ushiku, Masashi Fukayama,
The proliferation of Mott cells (plasma cells with multiple Russell bodies) is rarely observed in nonhematopoietic tumors, and no reports of this phenomenon in malignant epithelial neoplasms have been published. We present 2 cases of gastric carcinoma associated with prominent Mott cell proliferation. Histologically, both tumors consisted of extensive lymphoplasmacytic infiltration and numerous Mott cells with dysplastic epithelial cells. The epithelial cells showed overt cytologic atypia; infiltrating ...
Tópico(s): Polyomavirus and related diseases
2009 - Elsevier BV | Human Pathology
Rumi Hino, Hiroshi Uozaki, Noriko Murakami, Tetsuo Ushiku, Aya Shinozaki‐Ushiku, Shumpei Ishikawa, Teppei Morikawa, Takeo Nakaya, Takashi Sakatani, Kenzo Takada, Masashi Fukayama,
Abstract CpG island promoter methylation of tumor suppressor genes is one of the most characteristic abnormalities in EBV-associated gastric carcinoma (GC). Aberrant promoter methylation and expression loss of PTEN were evaluated in cancer tissues of GC by methylation-specific PCR and immunohistochemistry, respectively, showing that both abnormalities occurred concurrently in EBV-associated GC. PTEN abnormalities were reiterated in GC cell lines MKN-1 and MKN-7 infected with recombinant EBV, and ...
Tópico(s): RNA modifications and cancer
2009 - American Association for Cancer Research | Cancer Research
Tetsuo Ushiku, Hiroshi Uozaki, Aya Shinozaki‐Ushiku, Satoshi Ota, Keisuke Matsuzaka, Sachiyo Nomura, Michio Kaminishi, Hiroyuki Aburatani, Tatsuhiko Kodama, Masashi Fukayama,
Gypican-3 (GPC3) has been recognized as an oncofetal protein in hepatic neoplasms and yolk sac tumors. To characterize a distinct subgroup of gastric carcinoma (GC) expressing GPC3 (GPC3-GC), primary and metastatic GC tissues were evaluated by immunohistochemistry with special focus on their related entities: hepatoid, clear-cell, and alpha-fetoprotein-producing GC. GPC3-GC was defined as focal GPC3-GC when 10-49% of neoplastic cells were positive, and as diffuse GPC3-GC when more than 50% of cells ...
Tópico(s): Gastrointestinal Tumor Research and Treatment
2009 - Wiley | Cancer Science
Wing‐Yan Au, Annie Pang, Eunice C. Chan, Kent‐Man Chu, Tony W.H. Shek, Yok–Lam Kwong,
... cases.16Sudo M. Chong J.M. Sakuma K. Ushiku T. Uozaki H. Nagai H. Funata N. Matsumoto ... Scholar, 16Sudo M. Chong J.M. Sakuma K. Ushiku T. Uozaki H. Nagai H. Funata N. Matsumoto ...
Tópico(s): Eosinophilic Disorders and Syndromes
2005 - Elsevier BV | Gastroenterology