Produção Nacional
Milena Coelho Fernandes Caldato, Vânia T. Fernandes, Cláudio E. Kater,
Replacement schedules with hydrocortisone (HC) to treat 21OHD are generally unsatisfactory and partially successful regarding growth. Noncompliance is common since its short half-life requires TID administration. Even multiple daily HC doses do not reproduce cortisol chronobiology and may disturb hypothalamic-mediated rhythms. Because synthetic glucocorticoids could improve clinical control, we evaluated the possible benefits of a one-year treatment period with a single morning oral dose of prednisolone ( ...
Tópico(s): Metabolism and Genetic Disorders
2004 - Editora da Universidade de São Paulo | Arquivos Brasileiros de Endocrinologia & Metabologia
Mónica M. Costa, Vânia O. Fernandes, T. Ribeiro, Luís Serrano, Vânia Cardoso, Helena Santos, M.M. Lordelo, L.M.A. Ferreira, C.M.G.A. Fontes,
Anaerobic cellulolytic bacteria organize a comprehensive range of cellulases and hemicellulases in high molecular weight multienzyme complexes termed cellulosomes. Integration of cellulosomal components occurs via highly ordered protein-protein interactions between cohesins and dockerins. This paper reports the production of mini-cellulosomes containing one (GH16-1C) or three (GH16-3C) copies of Clostridium thermocellum glucanase 16A (CtGlc16A). Barley β-1,3-1,4-glucans are known to be antinutritive ...
Tópico(s): Food composition and properties
2014 - American Chemical Society | Journal of Agricultural and Food Chemistry
Vânia O. Fernandes, Mónica M. Costa, T. Ribeiro, Luís Serrano, Vânia Cardoso, Helena Santos, M.M. Lordelo, L.M.A. Ferreira, C.M.G.A. Fontes,
Barley-based diets contain a significant proportion of highly soluble 1,3-1,4-β-glucans that display anti-nutritive properties for monogastric animals, in particular for poultry. Cleavage of mixed linked glucans by the addition of exogenous enzymes leads to a significant reduction in the degree of polymerization of the polysaccharide and a consequent reduction of digesta viscosity. Plant cell wall 1,3-1,4-β-glucans may be hydrolyzed by 1,3-1,4-β-glucanases (EC 3.2.1.73) or by 1,4-β-glucanases (EC ...
Tópico(s): Food composition and properties
2015 - Elsevier BV | Animal Feed Science and Technology
B. Pinheiro, Harry J. Gilbert, K. Sakka, Kazuo Sakka, Vânia O. Fernandes, José A. M. Prates, Victor D. Alves, David N. Bolam, L.M.A. Ferreira, C.M.G.A. Fontes,
Cellulosomes, synthesized by anaerobic microorganisms such as Clostridium thermocellum, are remarkably complex nanomachines that efficiently degrade plant cell wall polysaccharides. Cellulosome assembly results from the interaction of type I dockerin domains, present on the catalytic subunits, and the cohesin domains of a large non-catalytic integrating protein that acts as a molecular scaffold. In general, type I dockerins contain two distinct cohesin-binding interfaces that appear to display identical ...
Tópico(s): Plant-Microbe Interactions and Immunity
2009 - Portland Press | Biochemical Journal
Produção Nacional
Revisado por pares
Vania Castriani Fernandes da Silva, Fabiano Jares Contesini, Patrícia de Oliveira Carvalho,
Considering the extraordinary microbial diversity and importance of fungi as enzyme producers, the search for new biocatalysts with special characteristics and possible applications in biocatalysis is of great interest. Here, we report the performance in the resolution of racemic ibuprofen of a native enantioselective lipase from Aspergillus niger, free and immobilized in five types of support (Accurel EP-100, Amberlite MB-1, Celite, Montmorillonite K10 and Silica gel). Amberlite MB-1 was found to ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2009 - Springer Science+Business Media | Journal of Industrial Microbiology & Biotechnology
Produção Nacional
Revisado por pares
Vânia T. Fernandes, Luciane M. Ribeiro-Neto, Samira Barbosa Lima, José Gilberto H. Vieira, Ieda T. N. Verreschi, Cláudio E. Kater,
21-Hydroxylase deficiency (21-OHD) is the most common form of congenital adrenal hyperplasia (CAH), followed by 11β-hydroxylase deficiency (11β-OHD). Diagnostic serum markers for these conditions are 17-hydroxyprogesterone (17-OHP) and 11- desoxycortisol (S), respectively. In 21-OHD, the large amounts of 17-OHP are further 11β-hydroxylated to form 21-deoxycortisol (21-DF), making it also an excellent marker of this disease. These steroids can be measured in blood by radioimmunoassay (RIA). In this paper, ...
Tópico(s): Growth Hormone and Insulin-like Growth Factors
2003 - Oxford University Press | Journal of Chromatographic Science
M.A.S. Correia, D. Wade Abbott, T.M. Gloster, Vânia O. Fernandes, José A. M. Prates, Cédric Montanier, Claire Dumon, Michael P. Williamson, Richard B. Tunnicliffe, Ziyuan Liu, J.E. Flint, G.J. Davies, Bernard Henrissat, Pedro M. Coutinho, C.M.G.A. Fontes, Harry J. Gilbert,
The deconstruction of the plant cell wall is an important biological process that is attracting considerable industrial interest, particularly in the bioenergy sector. Enzymes that attack the plant cell wall generally contain one or more noncatalytic carbohydrate binding modules (CBMs) that play an important targeting function. While CBMs that bind to the backbones of plant structural polysaccharides have been widely described, modules that recognize components of the vast array of decorations displayed ...
Tópico(s): Biochemical and Molecular Research
2010 - American Chemical Society | Biochemistry
Kimiya Mizutani, Vânia O. Fernandes, Shuichi Karita, Ana S. Luís, Makiko Sakka, Tetsuya Kimura, Adam Jackson, Xiaoyang Zhang, C.M.G.A. Fontes, Harry J. Gilbert, Kazuo Sakka,
ABSTRACT In general, cellulases and hemicellulases are modular enzymes in which the catalytic domain is appended to one or more noncatalytic carbohydrate binding modules (CBMs). CBMs, by concentrating the parental enzyme at their target polysaccharide, increase the capacity of the catalytic module to bind the substrate, leading to a potentiation in catalysis. Clostridium thermocellum hypothetical protein Cthe_0821, defined here as C. thermocellum Man5A, is a modular protein comprising an N-terminal ...
Tópico(s): Biofuel production and bioconversion
2012 - American Society for Microbiology | Applied and Environmental Microbiology
Soumyadeep Chakraborty, Vânia O. Fernandes, Fernando M. V. Dias, José A. M. Prates, L.M.A. Ferreira, C.M.G.A. Fontes, Arun Goyal, M.S.J. Centeno,
The cloning, expression and characterization of three cellulosomal pectinolytic enzymes viz., two variants of PL1 (PL1A and PL1B) and PL9 from Clostridium thermocellum was carried out. The comparison of the primary sequences of PL1A, PL1B and PL9 revealed that these proteins displayed considerable sequence similarities with family 1 and 9 polysaccharide lyases, respectively. PL1A, PL1B and PL9 are the putative catalytic domains of protein sequence ABN54148.1 and ABN53381.1 respectively. These two ...
Tópico(s): Advanced Cellulose Research Studies
2015 - Public Library of Science | PLoS ONE
Arun Dhillon, Vânia O. Fernandes, Fernando M. V. Dias, José A. M. Prates, L.M.A. Ferreira, C.M.G.A. Fontes, M.S.J. Centeno, Arun Goyal,
Tópico(s): Biofuel production and bioconversion
2016 - Springer Science+Business Media | Molecular Biotechnology
I. Venditto, Ana S. Luís, Maja G. Rydahl, Julia Schückel, Vânia O. Fernandes, Silvia Vidal-Melgosa, Pedro Bule, Arun Goyal, Virgínia M. R. Pires, Catarina G. Dourado, L.M.A. Ferreira, Pedro M. Coutinho, Bernard Henrissat, John Knox, Arnaud Baslé, Shabir Najmudin, Harry J. Gilbert, William G. T. Willats, C.M.G.A. Fontes,
Significance Plant cell wall (PCW) polysaccharide degradation is an important biological and industrial process. Noncatalytic carbohydrate binding modules (CBMs) fulfill a critical targeting function in PCW depolymerization. Ruminococcus flavefaciens synthesizes a highly efficient PCW degrading apparatus. Here, six previously unidentified R. flavefaciens CBM families were identified that targeted β-glucans, β-mannans, and pectins. Crystal structures of these CBMs revealed that recognition of β-glucans ...
Tópico(s): Enzyme Production and Characterization
2016 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Ana Filipa Sequeira, Jeremy Turchetto, Natalie J. Saez, Fanny Peysson, Laurie Ramond, Yoan Duhoo, Marilyne Blémont, Vânia O. Fernandes, L.T. Gama, L.M.A. Ferreira, Catarina I. P. I. Guerreiro, Nicolas Gilles, Hervé Darbon, C.M.G.A. Fontes, Renaud Vincentelli,
Animal venoms are large, complex libraries of bioactive, disulphide-rich peptides. These peptides, and their novel biological activities, are of increasing pharmacological and therapeutic importance. However, recombinant expression of venom peptides in Escherichia coli remains difficult due to the significant number of cysteine residues requiring effective post-translational processing. There is also an urgent need to develop high-throughput recombinant protocols applicable to the production of ...
Tópico(s): Bacillus and Francisella bacterial research
2017 - BioMed Central | Microbial Cell Factories
Produção Nacional
Revisado por pares
Fátima Aparecida Cardoso, Vitória Régia de Negreiros Cordeiro, Daniele Barreto de Lima, Bárbara de Caldas Melo, Raquel Nobre Barreto de Menezes, Ana Luiza Silva de Moulaz, Gabrielle Boff de Sá, Adalgisa Vânia Fernandes de Souza,
Trata-se de atividade desenvolvida por estudantes de enfermagem da Escola Superior de Ciências da Saúde, do Distrito Federal, durante o primeiro ano do curso. O objetivo foi realizar uma oficina de capacitação para 22 agentes comunitários de saúde com o intuito de esclarecê-los sobre o seu papel no trabalho comunitário e elevar sua autoestima. Utilizou-se a metodologia da problematização e diversas dinâmicas pedagógicas. Os temas abordados foram: direitos e deveres, atribuições, comunicação, ética, ...
Tópico(s): Palliative and Oncologic Care
2011 - Associação Brasileira de Enfermagem | Revista Brasileira de Enfermagem
Produção Nacional
Revisado por pares
Luiz Antônio Cuñha, Vânia Fernandes,
Este artigo aborda o ensino religioso nas escolas públicas, buscando identificar os posicionamentos de grupos político-ideológicos em torno da questão durante a tramitação do projeto da Lei de Diretrizes e Bases da Educação Nacional promulgada em 20 de dezembro de 1961. A análise tomou como fonte o Diário do Congresso Nacional no período entre 1948 e 1962. Constatou-se que, durante o longo período de tramitação do projeto na Câmara dos Deputados, diferentes pressões imprimiram suas marcas na LDB: ...
Tópico(s): History of Education Research in Brazil
2012 - UNIVERSIDADE DE SÃO PAULO | Educação e Pesquisa
Vânia Filipa Lima Fernandes, Christian Macaspac, Louise Weiwei Lu, Masato Yoshizawa,
Many animal species exhibit laterality in sensation and behavioral responses, namely, the preference for using either the left or right side of the sensory system. For example, some fish use their left eye when observing social stimuli, whereas they use their right eye to observe novel objects. However, it is largely unknown whether such laterality in sensory-behavior coupling evolves during rapid adaptation processes. Here, in the Mexican tetra, Astyanax mexicanus, we investigate the laterality ...
Tópico(s): Ichthyology and Marine Biology
2018 - Elsevier BV | Developmental Biology
Produção Nacional
Revisado por pares
Flávia A. Costa‐Barbosa, Vânia de Fátima Tonetto Fernandes, Valdemir Melechco Carvalho, Odete H. Nakamura, Vivian Oliveira Moura-Massari, Tânia A.S.S. Bachega, José Gilberto H. Vieira, Cláudio E. Kater,
Congenital adrenal hyperplasia caused by classic 21-hydroxylase deficiency (21OHD) is an autosomal recessive disorder with a high prevalence of asymptomatic heterozygote carriers (HTZ) in the general population, making case detection desirable by routine methodology. HTZ for classic and nonclassic (NC) forms have basal and ACTH-stimulated values of 17-hydroxyprogesterone (17OHP) that fail to discriminate them from the general population. 21-Deoxycortisol (21DF), an 11-hydroxylated derivative of 17OHP, ...
Tópico(s): Growth Hormone and Insulin-like Growth Factors
2010 - Wiley | Clinical Endocrinology
Vânia André, Auguste Fernandes, Pedro Paulo Santos, M. Teresa Duarte,
Five new multicomponent crystal forms of the neuroleptic drug gabapentin with isophthalic acid, phthalic acid, l-glutamine, and terephthalic and trimesic acids have been reported and are characterized by XRPD. While the single crystal structures of the first three compounds were not possible to determine, the latter two are further characterized by SCXRD, DSC, TGA, HSM, and IR. The strong homomeric R22(8) and heteromeric R42(8) synthons observed in the carboxylic acids and gabapentin, respectively, ...
Tópico(s): Coordination Chemistry and Organometallics
2011 - American Chemical Society | Crystal Growth & Design
Produção Nacional
Revisado por pares
Vânia de Fátima Tonetto Fernandes, Sofia Helena Valente de Lemos‐Marini, Hílton Kuperman, Luciane M. Ribeiro-Neto, Ieda T. N. Verreschi, Cláudio E. Kater,
21-Hydroxylase deficiency (21OHD) is the most common cause of congenital adrenal hyperplasia, followed in frequency by 11beta-hydroxylase deficiency (11betaOHD). Although the relative frequency of 11betaOHD is reported as between 3 and 5% of the cases, these numbers may have been somewhat underestimated.In 133 patients (89 females/44 males; 10 d-20.9 yr) with alleged classic 21OHD and five (three females/two males; 7.3-21 yr) with documented 11betaOHD, we measured serum 21-deoxycortisol (21DF), 17-hydroxyprogesterone ( ...
Tópico(s): Growth Hormone and Insulin-like Growth Factors
2006 - Oxford University Press | The Journal of Clinical Endocrinology & Metabolism
Sílvia Quaresma, Vânia André, Auguste Fernandes, M. Teresa Duarte,
Mechanochemistry an environment-friendly synthetic technique has been successfully used in the search for new metallopharmaceuticals, metallodrugs and bio-inspired metal-organic frameworks. These materials recently became highly promising on the pharmaceutical and medical field due to their ability to function as drug carriers, allowing a controlled drug delivery and release in addition to potential applications in imaging and magnetic resonance imaging for diagnostic and therapy. Here we refer ...
Tópico(s): Supramolecular Self-Assembly in Materials
2016 - Elsevier BV | Inorganica Chimica Acta
Vânia Cardoso, Elisabete Fernandes, Helena Santos, Benvindo Maçãs, M.M. Lordelo, L.T. Gama, L.M.A. Ferreira, C.M.G.A. Fontes, T. Ribeiro,
1. Endo-1,4-β-xylanase is known to improve the nutritive value of wheat-based diets for poultry by degrading dietary arabinoxylans. However, broilers' response to supplementation of wheat-based diets with exogenous endo-1,4-β-xylanase is not always observed.2. In this study, 108 different wheat lots were analysed for levels of extract viscosity as well as for endogenous endo-1,4-β-xylanase activity, and the impact of these two variables in animal performance was tested.3. Results revealed that endogenous ...
Tópico(s): Banana Cultivation and Research
2018 - Taylor & Francis | British Poultry Science
M.A.S. Correia, Virgínia M. R. Pires, Harry J. Gilbert, David N. Bolam, Vânia O. Fernandes, Victor D. Alves, José A.M. Prates, L.M.A. Ferreira, C.M.G.A. Fontes,
Noncatalytic carbohydrate-binding modules (CBMs), which are found in a variety of carbohydrate-degrading enzymes, have been grouped into sequence-based families. CBMs, by recruiting their appended enzymes onto the surface of the target substrate, potentiate catalysis particularly against insoluble substrates. Family 6 CBMs (CBM6s) display unusual properties in that they present two potential ligand-binding sites termed clefts A and B, respectively. Cleft B is located on the concave surface of the ...
Tópico(s): Microbial Metabolites in Food Biotechnology
2009 - Oxford University Press | FEMS Microbiology Letters
Produção Nacional
Revisado por pares
Fátima Meirelles, Vânia Maria Fernandes Teixeira, Tânia França,
RESUMO O artigo visou analisar o uso do aplicativo móvel, em especial, o WhatsApp, como ferramenta de gestão das ações de educação na saúde com ênfase na Educação Permanente em Saúde no estado do Rio de Janeiro. Estudo exploratório, de abordagem quantitativa, com uso de questionário on-line. Os participantes foram os gestores/responsáveis pelas ações de educação permanente. Os dados quantitativos foram tratados estatisticamente. Predominou o sexo feminino, a idade variou de 34 a 68 anos. O vínculo ...
Tópico(s): Education during COVID-19 pandemic
2022 - Centro Brasileiro de Estudos de Saúde | Saúde em Debate
Produção Nacional
Revisado por pares
Kenner Morais Fernandes, Vânia Déa de Carvalho, José Cal-Vidal,
ABSTRACT Some physical measurements were applied to silver bananas ( Musa paradisiaca ) during natural ripening, at room temperature. Changes in fruit weight, fruit diameter and skin thickness were determined, using gravimetric methods and a pachymeter. Both fruit pulp and skin experienced substantial weight loss during ripening. The average of skin thickness was reduced from almost 4.0 mm to 1.5 mm. The results were correlated to the most important chemical changes in the fruit, such as total acidity. ...
Tópico(s): Banana Cultivation and Research
1979 - Wiley | Journal of Food Science
Produção Nacional
Christiane Fernandes, Vania Gloria Sil, Patrícia Brasil, Maria Elizabeth Herdy Boec, Janice Mery Chicarino Coelho, Rita Maria Ribeiro No,
Problem statement: The principal aim was to evaluate the effects of dengue virus infection by dengue during pregnancy and analyze the histopathology of the placenta.Approach: A retrospective study was conducted with 13 pregnant women with dengue confirmed in the period from January-December 2002, during a widespread dengue type 3 epidemic in Rio de Janeiro.Maternal and newborn data were collected from patient files and medical records during hospitalization for research for identification of dengue ...
Tópico(s): Global Maternal and Child Health
2009 - | American Journal of Infectious Diseases
Produção Nacional
Revisado por pares
Éverton K. K. Fernandes, Vânia Rita Elias Pinheiro Bittencourt,
Tópico(s): Insect and Pesticide Research
2008 - Springer Science+Business Media | Experimental and Applied Acarology
Produção Nacional
Revisado por pares
Ana Paula Rodrigues Moraes, Isabele da Costa Angelo, Éverton K. K. Fernandes, Vânia Rita Elias Pinheiro Bittencourt, Avelino José Bittencourt,
Stomoxys calcitrans control is primarily based on the use of chemical products; however, new methods for control have been investigated. Biological control has been proposed as a promising alternative to traditional chemicals. The current study evaluated three methods in vitro to treat S. calcitrans eggs with the fungus Metarhizium anisopliae and assessed the virulence of this fungus to immature stages of this fly. In the first method of egg exposure, eggs were immersed in conidial suspensions and ...
Tópico(s): Insect Pest Control Strategies
2008 - Wiley | Annals of the New York Academy of Sciences
Produção Nacional
Revisado por pares
Rosana Colatino Soares Reis, Éverton K. K. Fernandes, Vânia Rita Elias Pinheiro Bittencourt,
Entomopathogenic fungi are promising alternatives to chemical acaricides in controlling tick populations; however, biological formulations are required to improve conidial performance of these pathogens in the field. This study evaluated the effect of in vitro treatment of Rhipicephalus sanguineus with conidial formulations of Beauveria bassiana (Bb) or Metarhizium anisopliae (Ma). Conidia were formulated (at 10(8) conidia mL(-1)) in (1) emulsifiable concentrate (EC), (2) polymerized cellulose gel (PCG), ...
Tópico(s): Insect Pest Control Strategies
2008 - Wiley | Annals of the New York Academy of Sciences
Produção Nacional
Revisado por pares
Maria Clara Porto Fernandes, Vânia Marli Schubert Backes,
Estudo qualitativo cujos sujeitos de pesquisa foram os membros de uma equipe da estratégia de saúde da família, e teve como objetivo conhecer suas perspectivas sobre a educação em saúde e problematizá-las por meio da concepção dialógica de Paulo Freire. Nos círculos de cultura, os quatro temas geradores: equipe de saúde da família; educação em saúde; prática da educação em saúde no trabalho da equipe e de que maneira melhorar as ações de educação em saúde existentes, foram codificados, descodificados ...
Tópico(s): Palliative and Oncologic Care
2010 - Associação Brasileira de Enfermagem | Revista Brasileira de Enfermagem
Margarida Isabel Barros Coelho Martins, Mariana Henriques, Ana Paula Dias Ribeiro, Ricardo M. Fernandes, Vânia Gonçalves, Álvaro Seabra, Joana Azeredo, Rosário Oliveira,
Background: The ability of the Candida species to colonize surfaces can be considered a risk factor for oral infection.Aims: The aim of this work was to establish oral Candida carriage in patients attending a dental clinic in Braga, Portugal.Methods: A total of 97 patients were analysed.Swab samples were collected, and directly cultured in CHROMagar Candida.Representative yeasts were identified by polymerase chain reaction.Results: From the samples analysed 54.6% (n=53) were Candida positive, and ...
Tópico(s): Oral microbiology and periodontitis research
2010 - Elsevier BV | Revista Iberoamericana de Micología
Produção Nacional
Revisado por pares
Anacélia Mendes Fernandes, Vânia Ribeiro da Cunha De Souza, Cornelis Robert Araújo Springer, Sérgio Vitorino Cardoso, Adriano Mota Loyola, Ricardo Alves Mesquita, Maria Cássia Ferreira de Aguiar,
Both hMSH2 and hMLH1 are integrated in the DNA mismatch repair (MMR) system. Reduced expression of these proteins has been reported in head and neck carcinoma; however, few studies have examined the normal levels of these proteins in oral tissues or investigated factors which could interfere with this expression. The aim of this study was to detect the expression pattern of hMSH2 and hMLH1 in normal oral mucosa and to correlate this with demographic data, smoking and local inflammation.The expression ...
Tópico(s): Cancer, Lipids, and Metabolism
2007 - International Institute of Anticancer Research (IIAR) Conferences 1997. Athens, Greece. Abstracts | Anticancer Research