Produção Nacional
Milena Coelho Fernandes Caldato, Vânia T. Fernandes, Cláudio E. Kater,
Replacement schedules with hydrocortisone (HC) to treat 21OHD are generally unsatisfactory and partially successful regarding growth. Noncompliance is common since its short half-life requires TID administration. Even multiple daily HC doses do not reproduce cortisol chronobiology and may disturb hypothalamic-mediated rhythms. Because synthetic glucocorticoids could improve clinical control, we evaluated the possible benefits of a one-year treatment period with a single morning oral dose of prednisolone ( ...
Tópico(s): Metabolism and Genetic Disorders
2004 - Editora da Universidade de São Paulo | Arquivos Brasileiros de Endocrinologia & Metabologia
Mónica M. Costa, Vânia O. Fernandes, T. Ribeiro, Luís Serrano, Vânia Cardoso, Helena Santos, M.M. Lordelo, L.M.A. Ferreira, C.M.G.A. Fontes,
Anaerobic cellulolytic bacteria organize a comprehensive range of cellulases and hemicellulases in high molecular weight multienzyme complexes termed cellulosomes. Integration of cellulosomal components occurs via highly ordered protein-protein interactions between cohesins and dockerins. This paper reports the production of mini-cellulosomes containing one (GH16-1C) or three (GH16-3C) copies of Clostridium thermocellum glucanase 16A (CtGlc16A). Barley β-1,3-1,4-glucans are known to be antinutritive ...
Tópico(s): Food composition and properties
2014 - American Chemical Society | Journal of Agricultural and Food Chemistry
Vânia O. Fernandes, Mónica M. Costa, T. Ribeiro, Luís Serrano, Vânia Cardoso, Helena Santos, M.M. Lordelo, L.M.A. Ferreira, C.M.G.A. Fontes,
Barley-based diets contain a significant proportion of highly soluble 1,3-1,4-β-glucans that display anti-nutritive properties for monogastric animals, in particular for poultry. Cleavage of mixed linked glucans by the addition of exogenous enzymes leads to a significant reduction in the degree of polymerization of the polysaccharide and a consequent reduction of digesta viscosity. Plant cell wall 1,3-1,4-β-glucans may be hydrolyzed by 1,3-1,4-β-glucanases (EC 3.2.1.73) or by 1,4-β-glucanases (EC ...
Tópico(s): Food composition and properties
2015 - Elsevier BV | Animal Feed Science and Technology
Ana Filipa Sequeira, Jeremy Turchetto, Natalie J. Saez, Fanny Peysson, Laurie Ramond, Yoan Duhoo, Marilyne Blémont, Vânia O. Fernandes, L.T. Gama, L.M.A. Ferreira, Catarina I. P. I. Guerreiro, Nicolas Gilles, Hervé Darbon, C.M.G.A. Fontes, Renaud Vincentelli,
Animal venoms are large, complex libraries of bioactive, disulphide-rich peptides. These peptides, and their novel biological activities, are of increasing pharmacological and therapeutic importance. However, recombinant expression of venom peptides in Escherichia coli remains difficult due to the significant number of cysteine residues requiring effective post-translational processing. There is also an urgent need to develop high-throughput recombinant protocols applicable to the production of ...
Tópico(s): Bacillus and Francisella bacterial research
2017 - BioMed Central | Microbial Cell Factories
I. Venditto, Ana S. Luís, Maja G. Rydahl, Julia Schückel, Vânia O. Fernandes, Silvia Vidal-Melgosa, Pedro Bule, Arun Goyal, Virgínia M. R. Pires, Catarina G. Dourado, L.M.A. Ferreira, Pedro M. Coutinho, Bernard Henrissat, John Knox, Arnaud Baslé, Shabir Najmudin, Harry J. Gilbert, William G. T. Willats, C.M.G.A. Fontes,
Significance Plant cell wall (PCW) polysaccharide degradation is an important biological and industrial process. Noncatalytic carbohydrate binding modules (CBMs) fulfill a critical targeting function in PCW depolymerization. Ruminococcus flavefaciens synthesizes a highly efficient PCW degrading apparatus. Here, six previously unidentified R. flavefaciens CBM families were identified that targeted β-glucans, β-mannans, and pectins. Crystal structures of these CBMs revealed that recognition of β-glucans ...
Tópico(s): Enzyme Production and Characterization
2016 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Arun Dhillon, Vânia O. Fernandes, Fernando M. V. Dias, José A. M. Prates, L.M.A. Ferreira, C.M.G.A. Fontes, M.S.J. Centeno, Arun Goyal,
Tópico(s): Biofuel production and bioconversion
2016 - Springer Science+Business Media | Molecular Biotechnology
Soumyadeep Chakraborty, Vânia O. Fernandes, Fernando M. V. Dias, José A. M. Prates, L.M.A. Ferreira, C.M.G.A. Fontes, Arun Goyal, M.S.J. Centeno,
The cloning, expression and characterization of three cellulosomal pectinolytic enzymes viz., two variants of PL1 (PL1A and PL1B) and PL9 from Clostridium thermocellum was carried out. The comparison of the primary sequences of PL1A, PL1B and PL9 revealed that these proteins displayed considerable sequence similarities with family 1 and 9 polysaccharide lyases, respectively. PL1A, PL1B and PL9 are the putative catalytic domains of protein sequence ABN54148.1 and ABN53381.1 respectively. These two ...
Tópico(s): Advanced Cellulose Research Studies
2015 - Public Library of Science | PLoS ONE
Kimiya Mizutani, Vânia O. Fernandes, Shuichi Karita, Ana S. Luís, Makiko Sakka, Tetsuya Kimura, Adam Jackson, Xiaoyang Zhang, C.M.G.A. Fontes, Harry J. Gilbert, Kazuo Sakka,
ABSTRACT In general, cellulases and hemicellulases are modular enzymes in which the catalytic domain is appended to one or more noncatalytic carbohydrate binding modules (CBMs). CBMs, by concentrating the parental enzyme at their target polysaccharide, increase the capacity of the catalytic module to bind the substrate, leading to a potentiation in catalysis. Clostridium thermocellum hypothetical protein Cthe_0821, defined here as C. thermocellum Man5A, is a modular protein comprising an N-terminal ...
Tópico(s): Biofuel production and bioconversion
2012 - American Society for Microbiology | Applied and Environmental Microbiology
M.A.S. Correia, D. Wade Abbott, T.M. Gloster, Vânia O. Fernandes, José A. M. Prates, Cédric Montanier, Claire Dumon, Michael P. Williamson, Richard B. Tunnicliffe, Ziyuan Liu, J.E. Flint, G.J. Davies, Bernard Henrissat, Pedro M. Coutinho, C.M.G.A. Fontes, Harry J. Gilbert,
The deconstruction of the plant cell wall is an important biological process that is attracting considerable industrial interest, particularly in the bioenergy sector. Enzymes that attack the plant cell wall generally contain one or more noncatalytic carbohydrate binding modules (CBMs) that play an important targeting function. While CBMs that bind to the backbones of plant structural polysaccharides have been widely described, modules that recognize components of the vast array of decorations displayed ...
Tópico(s): Biochemical and Molecular Research
2010 - American Chemical Society | Biochemistry
B. Pinheiro, Harry J. Gilbert, K. Sakka, Kazuo Sakka, Vânia O. Fernandes, José A. M. Prates, Victor D. Alves, David N. Bolam, L.M.A. Ferreira, C.M.G.A. Fontes,
Cellulosomes, synthesized by anaerobic microorganisms such as Clostridium thermocellum, are remarkably complex nanomachines that efficiently degrade plant cell wall polysaccharides. Cellulosome assembly results from the interaction of type I dockerin domains, present on the catalytic subunits, and the cohesin domains of a large non-catalytic integrating protein that acts as a molecular scaffold. In general, type I dockerins contain two distinct cohesin-binding interfaces that appear to display identical ...
Tópico(s): Plant-Microbe Interactions and Immunity
2009 - Portland Press | Biochemical Journal
Produção Nacional
Revisado por pares
Vania Castriani Fernandes da Silva, Fabiano Jares Contesini, Patrícia de Oliveira Carvalho,
Considering the extraordinary microbial diversity and importance of fungi as enzyme producers, the search for new biocatalysts with special characteristics and possible applications in biocatalysis is of great interest. Here, we report the performance in the resolution of racemic ibuprofen of a native enantioselective lipase from Aspergillus niger, free and immobilized in five types of support (Accurel EP-100, Amberlite MB-1, Celite, Montmorillonite K10 and Silica gel). Amberlite MB-1 was found to ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2009 - Springer Science+Business Media | Journal of Industrial Microbiology & Biotechnology
Produção Nacional
Revisado por pares
Vânia T. Fernandes, Luciane M. Ribeiro-Neto, Samira Barbosa Lima, José Gilberto H. Vieira, Ieda T. N. Verreschi, Cláudio E. Kater,
21-Hydroxylase deficiency (21-OHD) is the most common form of congenital adrenal hyperplasia (CAH), followed by 11β-hydroxylase deficiency (11β-OHD). Diagnostic serum markers for these conditions are 17-hydroxyprogesterone (17-OHP) and 11- desoxycortisol (S), respectively. In 21-OHD, the large amounts of 17-OHP are further 11β-hydroxylated to form 21-deoxycortisol (21-DF), making it also an excellent marker of this disease. These steroids can be measured in blood by radioimmunoassay (RIA). In this paper, ...
Tópico(s): Growth Hormone and Insulin-like Growth Factors
2003 - Oxford University Press | Journal of Chromatographic Science
Produção Nacional
Revisado por pares
Luiz Antônio Cuñha, Vânia Fernandes,
Este artigo aborda o ensino religioso nas escolas públicas, buscando identificar os posicionamentos de grupos político-ideológicos em torno da questão durante a tramitação do projeto da Lei de Diretrizes e Bases da Educação Nacional promulgada em 20 de dezembro de 1961. A análise tomou como fonte o Diário do Congresso Nacional no período entre 1948 e 1962. Constatou-se que, durante o longo período de tramitação do projeto na Câmara dos Deputados, diferentes pressões imprimiram suas marcas na LDB: ...
Tópico(s): History of Education Research in Brazil
2012 - UNIVERSIDADE DE SÃO PAULO | Educação e Pesquisa
Produção Nacional
Revisado por pares
Fátima Aparecida Cardoso, Vitória Régia de Negreiros Cordeiro, Daniele Barreto de Lima, Bárbara de Caldas Melo, Raquel Nobre Barreto de Menezes, Ana Luiza Silva de Moulaz, Gabrielle Boff de Sá, Adalgisa Vânia Fernandes de Souza,
Trata-se de atividade desenvolvida por estudantes de enfermagem da Escola Superior de Ciências da Saúde, do Distrito Federal, durante o primeiro ano do curso. O objetivo foi realizar uma oficina de capacitação para 22 agentes comunitários de saúde com o intuito de esclarecê-los sobre o seu papel no trabalho comunitário e elevar sua autoestima. Utilizou-se a metodologia da problematização e diversas dinâmicas pedagógicas. Os temas abordados foram: direitos e deveres, atribuições, comunicação, ética, ...
Tópico(s): Palliative and Oncologic Care
2011 - Associação Brasileira de Enfermagem | Revista Brasileira de Enfermagem
Vânia Filipa Lima Fernandes, Christian Macaspac, Louise Weiwei Lu, Masato Yoshizawa,
Many animal species exhibit laterality in sensation and behavioral responses, namely, the preference for using either the left or right side of the sensory system. For example, some fish use their left eye when observing social stimuli, whereas they use their right eye to observe novel objects. However, it is largely unknown whether such laterality in sensory-behavior coupling evolves during rapid adaptation processes. Here, in the Mexican tetra, Astyanax mexicanus, we investigate the laterality ...
Tópico(s): Ichthyology and Marine Biology
2018 - Elsevier BV | Developmental Biology
Vânia Cardoso, Elisabete Fernandes, Helena Santos, Benvindo Maçãs, M.M. Lordelo, L.T. Gama, L.M.A. Ferreira, C.M.G.A. Fontes, T. Ribeiro,
1. Endo-1,4-β-xylanase is known to improve the nutritive value of wheat-based diets for poultry by degrading dietary arabinoxylans. However, broilers' response to supplementation of wheat-based diets with exogenous endo-1,4-β-xylanase is not always observed.2. In this study, 108 different wheat lots were analysed for levels of extract viscosity as well as for endogenous endo-1,4-β-xylanase activity, and the impact of these two variables in animal performance was tested.3. Results revealed that endogenous ...
Tópico(s): Banana Cultivation and Research
2018 - Taylor & Francis | British Poultry Science
Sílvia Quaresma, Vânia André, Auguste Fernandes, M. Teresa Duarte,
Mechanochemistry an environment-friendly synthetic technique has been successfully used in the search for new metallopharmaceuticals, metallodrugs and bio-inspired metal-organic frameworks. These materials recently became highly promising on the pharmaceutical and medical field due to their ability to function as drug carriers, allowing a controlled drug delivery and release in addition to potential applications in imaging and magnetic resonance imaging for diagnostic and therapy. Here we refer ...
Tópico(s): Supramolecular Self-Assembly in Materials
2016 - Elsevier BV | Inorganica Chimica Acta
Vânia André, Auguste Fernandes, Pedro Paulo Santos, M. Teresa Duarte,
Five new multicomponent crystal forms of the neuroleptic drug gabapentin with isophthalic acid, phthalic acid, l-glutamine, and terephthalic and trimesic acids have been reported and are characterized by XRPD. While the single crystal structures of the first three compounds were not possible to determine, the latter two are further characterized by SCXRD, DSC, TGA, HSM, and IR. The strong homomeric R22(8) and heteromeric R42(8) synthons observed in the carboxylic acids and gabapentin, respectively, ...
Tópico(s): Coordination Chemistry and Organometallics
2011 - American Chemical Society | Crystal Growth & Design
Produção Nacional
Revisado por pares
Flávia A. Costa‐Barbosa, Vânia de Fátima Tonetto Fernandes, Valdemir Melechco Carvalho, Odete H. Nakamura, Vivian Oliveira Moura-Massari, Tânia A.S.S. Bachega, José Gilberto H. Vieira, Cláudio E. Kater,
Congenital adrenal hyperplasia caused by classic 21-hydroxylase deficiency (21OHD) is an autosomal recessive disorder with a high prevalence of asymptomatic heterozygote carriers (HTZ) in the general population, making case detection desirable by routine methodology. HTZ for classic and nonclassic (NC) forms have basal and ACTH-stimulated values of 17-hydroxyprogesterone (17OHP) that fail to discriminate them from the general population. 21-Deoxycortisol (21DF), an 11-hydroxylated derivative of 17OHP, ...
Tópico(s): Growth Hormone and Insulin-like Growth Factors
2010 - Wiley | Clinical Endocrinology
Produção Nacional
Revisado por pares
Vânia de Fátima Tonetto Fernandes, Sofia Helena Valente de Lemos‐Marini, Hílton Kuperman, Luciane M. Ribeiro-Neto, Ieda T. N. Verreschi, Cláudio E. Kater,
21-Hydroxylase deficiency (21OHD) is the most common cause of congenital adrenal hyperplasia, followed in frequency by 11beta-hydroxylase deficiency (11betaOHD). Although the relative frequency of 11betaOHD is reported as between 3 and 5% of the cases, these numbers may have been somewhat underestimated.In 133 patients (89 females/44 males; 10 d-20.9 yr) with alleged classic 21OHD and five (three females/two males; 7.3-21 yr) with documented 11betaOHD, we measured serum 21-deoxycortisol (21DF), 17-hydroxyprogesterone ( ...
Tópico(s): Growth Hormone and Insulin-like Growth Factors
2006 - Oxford University Press | The Journal of Clinical Endocrinology & Metabolism
Produção Nacional
Revisado por pares
Fátima Meirelles, Vânia Maria Fernandes Teixeira, Tânia França,
RESUMO O artigo visou analisar o uso do aplicativo móvel, em especial, o WhatsApp, como ferramenta de gestão das ações de educação na saúde com ênfase na Educação Permanente em Saúde no estado do Rio de Janeiro. Estudo exploratório, de abordagem quantitativa, com uso de questionário on-line. Os participantes foram os gestores/responsáveis pelas ações de educação permanente. Os dados quantitativos foram tratados estatisticamente. Predominou o sexo feminino, a idade variou de 34 a 68 anos. O vínculo ...
Tópico(s): Education during COVID-19 pandemic
2022 - Centro Brasileiro de Estudos de Saúde | Saúde em Debate
M.A.S. Correia, Virgínia M. R. Pires, Harry J. Gilbert, David N. Bolam, Vânia O. Fernandes, Victor D. Alves, José A.M. Prates, L.M.A. Ferreira, C.M.G.A. Fontes,
Noncatalytic carbohydrate-binding modules (CBMs), which are found in a variety of carbohydrate-degrading enzymes, have been grouped into sequence-based families. CBMs, by recruiting their appended enzymes onto the surface of the target substrate, potentiate catalysis particularly against insoluble substrates. Family 6 CBMs (CBM6s) display unusual properties in that they present two potential ligand-binding sites termed clefts A and B, respectively. Cleft B is located on the concave surface of the ...
Tópico(s): Microbial Metabolites in Food Biotechnology
2009 - Oxford University Press | FEMS Microbiology Letters
Produção Nacional
Revisado por pares
A.C.L. de Paula, Julliane Dutra Medeiros, Gabriel da Rocha Fernandes, Vânia Lúcia da Silva, Cláudio Galuppo Diniz,
The knowledge about microbiota from cheeses plays a key role in safety improvement and quality enhancement of the final product. We used a high throughput sequencing methodology to provide insights into the microbial composition of Minas Frescal Cheese (MFC). The results obtained from the analysis of 28 cheese samples showed that cheeses were brand-clustered, exhibiting unique bacterial profiles which varied in diversity and abundance of taxa. Proteobacteria dominated in all samples, corresponding ...
Tópico(s): Gut microbiota and health
2020 - Elsevier BV | LWT
Produção Nacional
Revisado por pares
Joelise Alencar Figueira ANGELOTTI, Fernanda Furlan Gonçalves Dias, Hélia Harumi Sato, Pedro Fernandes, Vânia Mayumi Nakajima, Juliana Alves Macedo,
Tópico(s): Diet, Metabolism, and Disease
2020 - Springer Science+Business Media | Applied Biochemistry and Biotechnology
Produção Nacional
Revisado por pares
Amanda R. C. Corval, Emily Mesquita, Thaís Almeida Corrêa, Cárita S. Ribeiro-Silva, Ricardo de Oliveira Barbosa Bitencourt, Éverton K. K. Fernandes, Vânia Rita Elias Pinheiro Bittencourt, Donald W. Roberts, Patrícia Silva Gôlo,
The aim of the present study was to analyze ten native Metarhizium spp. isolates as to their UV-B tolerances. Comparisons included: different fungal propagules (conidia, blastospores, or microsclerotia [MS]); conidia in aqueous suspensions or in 10% mineral oil-in-water emulsions; and conidia mixed with different types of soil. The UV-B effect was expressed as the germination of conidia or culturability of blastospores and MS relative to nongerminated propagules. Metarhizium anisopliae LCM S05 exhibited ...
Tópico(s): Insect Resistance and Genetics
2020 - Wiley | Journal of Basic Microbiology
Marina V. Kirillova, Tiago A. Fernandes, Vânia André, Alexander M. Kirillov,
Three new copper( ii ) coordination compounds with aminoalcohol and carboxylate type ligands were self-assembled, fully characterized, and applied as efficient homogeneous catalysts for the mild oxidation and carboxylation of saturated hydrocarbons.
Tópico(s): Catalytic C–H Functionalization Methods
2019 - Royal Society of Chemistry | Organic & Biomolecular Chemistry
Produção Nacional
Revisado por pares
Ângela Graciela Deliga Schröder, Fernando Henrique Westphalen, Júlio César Schröder, Ângela Fernandes, Vânia Portela Ditzel Westphalen,
Abstract Introduction The aim of this study was to compare the sensitivity and specificity of digital periapical radiography and cone-beam computed tomographic (CBCT) imaging in the detection of natural and simulated external root resorptions (ERRs) with micro–computed tomographic (micro-CT) imaging as the reference standard. Methods One hundred twenty-six teeth were scanned using the SkyScan 1172 micro-CT scanner (Bruker microCT, Kontich, Belgium), and the images were evaluated using NRecon software (Bruker ...
Tópico(s): Radiology practices and education
2018 - Elsevier BV | Journal of Endodontics
Produção Nacional
Revisado por pares
Ana Flávia Tonelli Fernandes, Vânia Santos Braz, Anelize Bauermeister, Jonas Augusto Rizzato Paschoal, Norberto Peporine Lopes, Eliana Guedes Stehling,
Atrazine is a herbicide used worldwide to control weeds in maize, sorghum and sugarcane crops. This study presents two bacteria isolated from Brazilian soil samples with atrazine application history. The isolates, identified as Pseudomonas sp. and Achromobacter sp., showed potential to degrade atrazine in solid medium. Pseudomonas sp. exhibited all the six atz genes that encode enzymes of the degradation process. This isolate was capable of degrading 99% of atrazine in vitro after 24 h of incubation. ...
Tópico(s): Environmental Toxicology and Ecotoxicology
2018 - Elsevier BV | International Biodeterioration & Biodegradation
Mariana Fernandes, Vânia T. Freitas, Sónia Pereira, R. Leones, M.M. Silva, Luís D. Carlos, Elvira Fortunato, Rute A. S. Ferreira, Rosa Rego, V. de Zea Bermudez,
To address the challenges of the next generation of smart windows for energy-efficient buildings, new electrochromic devices (ECDs) are introduced. These include indium molybdenum oxide (IMO), a conducting oxide transparent in the near-infrared (NIR) region, and a NIR-emitting electrolyte. The novel electrolytes are based on a sol-gel-derived di-urethane cross-linked siloxane-based host structure, including short chains of poly (ε-caprolactone) (PCL(530) (where 530 represents the average molecular weight ...
Tópico(s): Gas Sensing Nanomaterials and Sensors
2018 - Multidisciplinary Digital Publishing Institute | Energies
Julie Chesné, Vânia Cardoso, Henrique Veiga‐Fernandes,
Mucosal barriers constitute major body surfaces that are in constant contact with the external environment. Mucosal sites are densely populated by a myriad of distinct neurons and immune cell types that sense, integrate and respond to multiple environmental cues. In the recent past, neuro-immune interactions have been reported to play central roles in mucosal health and disease, including chronic inflammatory conditions, allergy and infectious diseases. Discrete neuro-immune cell units act as building ...
Tópico(s): IL-33, ST2, and ILC Pathways
2018 - Elsevier BV | Mucosal Immunology