Produção Nacional
Revisado por pares
Carlos Camargo Alberts, João Tadeu Ribeiro‐Paes, G. Aranda-Selverio, Jeny R. Cursino‐Santos, Vanessa Roma Moreno-Cotulio, A. L. de Oliveira, B.F.M.M. Porchia, Wagner Ferreira dos Santos, Elineide Barbosa de Souza,
Wild felids and canids are usually the main predators in the food chains where they dwell and are almost invisible to behavior and ecology researchers. Due to their grooming behavior, they tend to swallow shed hair, which shows up in the feces. DNA found in hair shafts can be used in molecular studies that can unravel, for instance, genetic variability, reproductive mode and family structure, and in some species, it is even possible to estimate migration and dispersion rates in given populations. ...
Tópico(s): Forensic and Genetic Research
2010 - Research Foundation of Ribeirão Preto | Genetics and Molecular Research
Produção Nacional
Revisado por pares
Daiane Santos Rosa, Silmara Aparecida Faggion, Amanda Salomão Gavin, Maicon Anderson de Souza, Helene Fachim, Wagner Ferreira dos Santos, Ana Maria Soares Pereira, Alexandra Olímpio Siqueira Cunha, Renê Oliveira Beleboni,
In this study, we isolated the alkaloid erysothrine from the hydroalcoholic extract of flowers from E. mulungu and screened for its anticonvulsant and anxiolytic actions based on neuroethological and neurochemical experiments. Our results showed that the administration of erysothrine inhibited seizures evoked by bicuculline, PTZ, NMDA and most remarkably, kainic acid. Also, erysothrine induced an increase in the number of entries but not in the time spent in the open arms of the EPM. However, we ...
Tópico(s): Plant chemical constituents analysis
2012 - Elsevier BV | Epilepsy & Behavior
Produção Nacional
Revisado por pares
Alexandra Olímpio Siqueira Cunha, Márcia Renata Mortari, José Luiz Liberato, Wagner Ferreira dos Santos,
Cell damage and spatial localization deficits are often reported as long-term consequences of pilocarpine-induced status epilepticus. In this study, we investigated the neuroprotective effects of repeated drug administration after long-lasting status epilepticus. Groups of six to eight Wistar rats received microinjections of pilocarpine (2.4 mg/microl, 1 microl) in the right dorsal hippocampus to induce a status epilepticus, which was attenuated by thiopental injection (35 mg/kg, i.p.) 3 hrs after ...
Tópico(s): Neurogenesis and neuroplasticity mechanisms
2009 - Wiley | Basic & Clinical Pharmacology & Toxicology
Produção Nacional
Revisado por pares
Paulo Louzada‐Júnior, J. J. Dias, Wagner Ferreira dos Santos, João José Lachat, H. F. Bradford, Joaquim Coutinho‐Netto,
Abstract A rabbit eye model of neural ischaemia is described that uses an increased pressure in the anterior eye chamber to block the capillary supply to the retina. A microdialysis probe placed very close to the retinal surface was used to monitor release of amino acids during ischaemia. A large (two‐ to threefold) increase in the release of glutamate and O ‐phosphoserine (twofold), but not of six other amino acids monitored, occurred during initial ischaemia. During reperfusion after release of intraocular ...
Tópico(s): Photoreceptor and optogenetics research
1992 - Wiley | Journal of Neurochemistry
Produção Nacional
Revisado por pares
M.A.R. Cairrão, Alessandra Mussi Ribeiro, Andréa Baldocchi Pizzo, Andréia C. K. Fontana, Renê Oliveira Beleboni, Joaquim Coutinho‐Netto, Antônio Miranda, Wagner Ferreira dos Santos,
In this article we describe an in vivo anticonvulsant effect from denatured crude venom and partially isolated fractions from two spiders: Parawixia bistriata and Scaptocosa raptoria. Intracerebroventricular injections of these venoms and fractions abolished rat convulsive tonic-clonic seizures induced by picrotoxin, bicuculline and pentylenetetrazole, and also, inhibited GABA uptake in synaptosomes of rat cerebral cortex. The venoms described in this work seems to be promising tools for the study ...
Tópico(s): Ion channel regulation and function
2002 - Taylor & Francis | Pharmaceutical Biology
Produção Nacional
Revisado por pares
Volker Herzig, Richard J. Ward, Wagner Ferreira dos Santos,
Venom from male and female specimens of the medically most important Brazilian Ctenidae spider Phoneutria nigriventer (Keyserling, 1891) has been compared. Males showed a slightly higher venom yield (386 μg) than equal sized females (296 μg), while adult females showed nearly a three times higher venom yield (1079 μg). High-pressure liquid-chromatography analyses revealed differences in the venom composition between males and females. A single peak in HPLC (peak 11) was only detected in venom from females ...
Tópico(s): Venomous Animal Envenomation and Studies
2002 - Elsevier BV | Toxicon
Produção Nacional
Revisado por pares
Andréia C. K. Fontana, Renato Guizzo, Renê Oliveira Beleboni, Antonio Renato Meirelles e Silva, Norberto Cysne Coimbra, Susan Amara, Wagner Ferreira dos Santos, Joaquim Coutinho‐Netto,
(1) In this study, we examined the effects of crude venom from the spider Parawixia bistriata on glutamate and GABA uptake into synaptosomes prepared from rat cerebral cortex. Addition of venom to cortical synaptosomes stimulated glutamate uptake and inhibited GABA uptake in a concentration-dependent manner. (2) The venom was fractionated using reverse-phase high-performance liquid chromatography on a preparative column. The fraction that retained glutamate uptake-stimulating activity was further ...
Tópico(s): Venomous Animal Envenomation and Studies
2003 - Wiley | British Journal of Pharmacology
Produção Nacional
Revisado por pares
Rejane Barbosa de Oliveira, Daniela Aparecida Chagas‐Paula, Bruno Alves Rocha, João José Franco, Leonardo Gobbo‐Neto, Sérgio Akira Uyemura, Wagner Ferreira dos Santos, Fernando B. Da Costa,
Yacon [Smallanthus sonchifolius (Poepp. & Endl.) H. Robinson, Asteraceae] is an Andean species that has traditionally been used as an anti-diabetic herb in several countries around the world, including Brazil. Its hypoglycaemic action has recently been demonstrated in normal and diabetic rats. However, studies about the safety of prolonged oral consumption of yacon leaf extracts are lacking. Thus, this work was undertaken to evaluate the repeated-dose toxicity of three extracts from yacon leaves: the ...
Tópico(s): Botanical Research and Applications
2010 - Elsevier BV | Journal of Ethnopharmacology
Produção Nacional
Revisado por pares
Sérgio L.C. Ferreira, Jaílson B. de Andrade, Maria das Graças A. Korn, M PEREIRA, Valfredo Azevedo Lemos, Wagner Ferreira dos Santos, Frederico de Medeiros Rodrigues, Anderson Santos Souza, Hilda Silva Ferreira, E DASILVA,
Spectrometric techniques for the analysis of trace cadmium have developed rapidly due to the increasing need for accurate measurements at extremely low levels of this element in diverse matrices. This review covers separation and preconcentration procedures, such as electrochemical deposition, precipitation, coprecipitation, solid phase extraction, liquid–liquid extraction (LLE) and cloud point extraction (CPE), and consider the features of the their application with several spectrometric techniques.
Tópico(s): Electrochemical Analysis and Applications
2007 - Elsevier BV | Journal of Hazardous Materials
Andréia C. K. Fontana, Renê Oliveira Beleboni, Marcin W. Wojewodzic, Wagner Ferreira dos Santos, Joaquim Coutinho‐Netto, Nina Julie Grutle, Spencer D. Watts, Niels C. Danbolt, Susan Amara,
Previous studies have shown that a compound purified from the spider Parawixia bistriata venom stimulates the activity of glial glutamate transporters and can protect retinal tissue from ischemic damage. To understand the mechanism by which this compound enhances transport, we examined its effects on the functional properties of glutamate transporters after solubilization and reconstitution in liposomes and in transfected COS-7 cells. Here, we demonstrate in both systems that Parawixin1 promotes ...
Tópico(s): Neurobiology and Insect Physiology Research
2007 - American Society for Pharmacology and Experimental Therapeutics | Molecular Pharmacology
Produção Nacional
Revisado por pares
Márcia Renata Mortari, Alexandra Olímpio Siqueira Cunha, Luzitano Brandão Ferreira, Wagner Ferreira dos Santos,
Invertebrate venoms have attracted considerable interest as a potential source of bioactive substances, especially neurotoxins. These molecules have proved to be extremely useful tools for the understanding of synaptic transmission events, and they have contributed to the design of novel drugs for the treatment of neurological disorders and pain. In this context, as epilepsy involves neuronal substrates, which are sites of action of many neurotoxins; venoms may be particularly useful for antiepileptic ...
Tópico(s): Ion channel regulation and function
2007 - Elsevier BV | Pharmacology & Therapeutics
Produção Nacional
Revisado por pares
Márcia Renata Mortari, Alexandra Olímpio Siqueira Cunha, Ruither Oliveira Gomes Carolino, Joaquim Coutinho‐Netto, José Carlos Tomaz, Norberto Peporine Lopes, Norberto Cysne Coimbra, Wagner Ferreira dos Santos,
In this work, a neuroactive peptide from the venom of the neotropical wasp Polybia occidentalis was isolated and its anti-nociceptive effects were characterized in well-established pain induction models.Wasp venom was analysed by reverse-phase HPLC and fractions screened for anti-nociceptive activity. The structure of the most active fraction was identified by electron-spray mass spectrometry (ESI-MS/MS) and it was further assessed in two tests of anti-nociceptive activity in rats: the hot plate ...
Tópico(s): Healthcare and Venom Research
2007 - Wiley | British Journal of Pharmacology
Produção Nacional
Revisado por pares
Helene Fachim, Alexandra Olímpio Siqueira Cunha, Adriana Pereira, Renê Oliveira Beleboni, Leonardo Gobbo‐Neto, Norberto Peporine Lopes, Joaquim Coutinho‐Netto, Wagner Ferreira dos Santos,
The neurobiological activity of Parawixin 10, isolated from Parawixia bistriata spider venom, was investigated. Cannulas were implanted in the lateral ventricles of Wistar rats (200–250 g, n=6–8 per group) to perform anticonvulsant and behavioral assays, and synaptosomes from cerebral cortices of male Wistar rats were used for neurochemical studies. The results indicate that pretreatment with Parawixin 10 prevents the onset of seizures induced with kainic acid, N-methyl-D-aspartate, and pentylenetetrazole ...
Tópico(s): Neuroscience and Neuropharmacology Research
2011 - Elsevier BV | Epilepsy & Behavior
Produção Nacional
Revisado por pares
Silmara Aparecida Faggion, Alexandra Olímpio Siqueira Cunha, Helene Fachim, Amanda Salomão Gavin, Wagner Ferreira dos Santos, Ana Maria Soares Pereira, Renê Oliveira Beleboni,
Neural mechanisms underlying the onset and maintenance of epileptic seizures involve alterations in inhibitory and/or excitatory neurotransmitter pathways. Thus, the prospecting of novel molecules from natural products that target both inhibition and excitation systems has deserved interest in the rational design of new anticonvulsants. We isolated the alkaloids (+)-erythravine and (+)-11-α-hydroxy-erythravine from the flowers of Erythrina mulungu and evaluated the action of these compounds against chemically ...
Tópico(s): Biochemical Analysis and Sensing Techniques
2011 - Elsevier BV | Epilepsy & Behavior
Produção Nacional
Revisado por pares
Renê Oliveira Beleboni, Renato Guizzo, Andréia C. K. Fontana, Andréa Baldocchi Pizzo, Ruither Oliveira Gomes Carolino, Leonardo Gobbo‐Neto, Norberto Peporine Lopes, Joaquim Coutinho‐Netto, Wagner Ferreira dos Santos,
The major contribution of this work is the isolation of a neuroprotective compound referred to as 2-amino-5-ureidopentanamide (FrPbAII) (M(r) = 174) from Parawixia bistriata spider venom and an investigation of its mode of action. FrPbAII inhibits synaptosomal GABA uptake in a dose-dependent manner and probably does not act on Na(+), K(+), and Ca(2+) channels, GABA(B) receptors, or gamma-aminobutyrate:alpha-ketoglutarate aminotransferase enzyme; therefore, it is not directly dependent on these structures ...
Tópico(s): Neuroscience and Neuropharmacology Research
2006 - American Society for Pharmacology and Experimental Therapeutics | Molecular Pharmacology
Produção Nacional
Revisado por pares
José Luiz Liberato, Alexandra Olímpio Siqueira Cunha, Márcia Renata Mortari, Erica A. Gelfuso, Renê Oliveira Beleboni, Joaquim Coutinho‐Netto, Wagner Ferreira dos Santos,
This study was aimed at determining the effects of FrPbAII (174 Da), a novel isolated component from Parawixia bistriata spider venom, in the CNS of Wistar rats. Considering that FrPbAII inhibits the high affinity GABAergic uptake in a dose-dependent manner, its anxiolytic and anticonvulsant effects were analyzed in well-established animal models. Injection of FrPbAII in the rat hippocampus induced a marked anxiolytic effect, increasing the occupancy in the open arms of the elevated plus maze (EC( ...
Tópico(s): Neuroscience and Neuropharmacology Research
2006 - Elsevier BV | Brain Research
Produção Nacional
Revisado por pares
Marcelo Cairrão Araújo Rodrigues, Luís Henrique Souza Guimarães, José Luiz Liberato, Maria de Lourdes Teixeira de Moraes Polizeli, Wagner Ferreira dos Santos,
This present study describes the isolation of a high molecular weight fraction (F1) from the venom of the social spider Parawixia bistriata, by gel filtration and also its subfractions by further purification with affinity chromatography on a Concanavalin A-Sepharose column. Acid and an alkaline phosphatase activities were found in fractions. The effects of pH, temperature and metallic ions on these activities were evaluated. Optimal temperature for both enzymes was 55 °C and optimal pH was 5.0 and ...
Tópico(s): Ion channel regulation and function
2006 - Elsevier BV | Toxicon
Produção Nacional
Revisado por pares
Erica A. Gelfuso, Alexandra Olímpio Siqueira Cunha, Márcia Renata Mortari, José Luiz Liberato, Karina Haddad Paraventi, Renê Oliveira Beleboni, Joaquim Coutinho‐Netto, Norberto Peporine Lopes, Wagner Ferreira dos Santos,
The aims of the present study were to investigate the anticonvulsant activity and behavioral toxicity of FrPbAII using freely moving Wistar rats. Moreover, the effectiveness of this compound against chemical convulsants was compared to that of the inhibitor of the GABAergic uptake, nipecotic acid. Our results show that FrPbAII was effective against seizures induced by the i.c.v. injection of pilocarpine (ED(50) = 0.05 microg/animal), picrotoxin (ED(50) = 0.02 microg/animal), kainic acid (ED(50) = 0.2 microg/ ...
Tópico(s): Cholinesterase and Neurodegenerative Diseases
2006 - Elsevier BV | Life Sciences
Produção Nacional
Revisado por pares
Javier Marín‐Prida, Giselle Pentón‐Rol, Fernando Postalli Rodrigues, Luciane C. Alberici, Karina Stringhetta, Andréia Machado Leopoldino, Zeki Naal, Ana Cristina Morseli Polizello, Alexey Llopiz, Marcela N. Rosa, José Luiz Liberato, Wagner Ferreira dos Santos, Sérgio Akira Uyemura, Eduardo Pentón‐Arias, Carlos Curti, Gilberto L. Pardo‐Andreu,
Oxidative stress and mitochondrial impairment are essential in the ischemic stroke cascade and eventually lead to tissue injury. C-Phycocyanin (C-PC) has previously been shown to have strong antioxidant and neuroprotective actions. In the present study, we assessed the effects of C-PC on oxidative injury induced by tert-butylhydroperoxide (t-BOOH) in SH-SY5Y neuronal cells, on transient ischemia in rat retinas, and in the calcium/phosphate-induced impairment of isolated rat brain mitochondria (RBM). ...
Tópico(s): Neurological Disease Mechanisms and Treatments
2012 - Elsevier BV | Brain Research Bulletin
Produção Nacional
Revisado por pares
Alexandra Olímpio Siqueira Cunha, Márcia Renata Mortari, Luciana Coutinho de Oliveira, Ruither Oliveira Gomes Carolino, Joaquim Coutinho‐Netto, Wagner Ferreira dos Santos,
Venoms of spiders and wasps are well recognized to present high affinity to the central nervous tissue of many mammalian species. Here we describe the effects of direct exposure of rat (Rattus norvegicus) brains to the crude and denatured venom of the Brazilian social wasp Polybia ignobilis. Lower doses of crude venom injected via intracerebroventricular (i.c.v.) inhibited the exploratory activity of animals, while higher doses provoked severe generalized tonic–clonic seizures, with hind limb extension. ...
Tópico(s): Venomous Animal Envenomation and Studies
2005 - Elsevier BV | Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology
Produção Nacional
Revisado por pares
Márcia Renata Mortari, Alexandra Olímpio Siqueira Cunha, Luciana G. de Oliveira, Eveline Bis Vieira, Erica A. Gelfuso, Joaquim Coutinho‐Netto, Wagner Ferreira dos Santos,
Abstract: Several investigations demonstrate that neurotoxins isolated from venoms of spiders and wasps may exert specific and selective activity on structures of the mammalian CNS. In the present work we examine the neurological effects of the low molecular weight compounds of the denatured venom of the neotropical social wasp Polybia occidentalis in freely moving rats. Central administration of denatured venom decreased the duration of exploratory, elevation and grooming behaviours on the open ...
Tópico(s): Insect and Pesticide Research
2005 - Wiley | Basic & Clinical Pharmacology & Toxicology
Produção Nacional
Revisado por pares
Ruither Oliveira Gomes Carolino, Renê Oliveira Beleboni, Ângela A Pizzo, Fabrizio Vecchio, Norberto Garcia‐Cairasco, Miguel Moysés Neto, Wagner Ferreira dos Santos, Joaquim Coutinho‐Netto,
We obtained a neurotoxic fraction (AcTx) from star fruit (Averrhoa carambola) and studied its effects on GABAergic and glutamatergic transmission systems. AcTx had no effect on GABA/glutamate uptake or release, or on glutamate binding. However, it specifically inhibited GABA binding in a concentration-dependent manner (IC50 = 0.89 μM). Video-electroencephalogram recordings demonstrated that following cortical administration of AcTx, animals showed behavioral changes, including tonic-clonic seizures, ...
Tópico(s): Medicinal Plants and Neuroprotection
2005 - Elsevier BV | Neurochemistry International
Produção Nacional
Revisado por pares
Lúcia de Paula, Wagner Ferreira dos Santos, Adriana Malheiro, Daniela Carlos, Lúcia Helena Faccioli,
Hymenoptera stings are quite common and can cause inflammatory reactions (in nonallergic individuals) or serious reactions (in allergic individuals). Hymenoptera venom contains histamine, vasoactive kinins, serotonin, phospholipase A, phospholipase B, hyaluronidase, antigen 5 and mastoparans. Some of these substances are responsible for local pain, as well as for activation of complement and endothelial cells. Polybia paulista is a wasp typically found in the state of São Paulo, Brazil. In the present ...
Tópico(s): Insect and Pesticide Research
2005 - Elsevier BV | International Immunopharmacology
Produção Nacional
Revisado por pares
Andréa Baldocchi Pizzo, Renê Oliveira Beleboni, Andréia C. K. Fontana, Alessandra Mussi Ribeiro, Antônio Miranda, Joaquim Coutinho‐Netto, Wagner Ferreira dos Santos,
It has previously been shown that the denatured crude extract of Agelaia vicina wasp venom inhibits glutamate and GABA uptake in rat cerebral cortex synaptosomes. To identify the components responsible for these effects, the neurotoxin AvTx 7 (molecular weight of 1210 Da) was isolated from A. vicina venom and its effects on glutamate neurotransmission investigated. AvTx 7 inhibits glutamate uptake in a dose-dependent and uncompetitive manner. AvTx 7 was found to stimulate the glutamate release in ...
Tópico(s): Ion channel regulation and function
2004 - Wiley | Journal of Biochemical and Molecular Toxicology
Produção Nacional
Revisado por pares
Luciana G. de Oliveira, Alexandra Olímpio Siqueira Cunha, Márcia Renata Mortari, Andréa Baldocchi Pizzo, Antônio Miranda, Norberto Cysne Coimbra, Wagner Ferreira dos Santos,
Several investigations have provided information that defensive behaviors evoked by stimulation of deep layers of the superior colliculus (dlSC) are subjected to inhibitory nigral modulation. This inhibition is made mainly through GABAergic neurons from substantia nigra, pars reticulata (SNpr), that sends outputs toward neural networks of the deep layers of the superior colliculus and dorsal periaqueductal gray matter involved with the organization of fear-like responses. In this work, we compared ...
Tópico(s): Insect and Arachnid Ecology and Behavior
2004 - Elsevier BV | Brain Research
Produção Nacional
Revisado por pares
Volker Herzig, Richard J. Ward, Wagner Ferreira dos Santos,
Tópico(s): Beetle Biology and Toxicology Studies
2004 - Elsevier BV | Toxicon
Produção Nacional
Revisado por pares
Renê Oliveira Beleboni, Ruither Oliveira Gomes Carolino, Andréa Baldocchi Pizzo, Lissandra Castellan-Baldan, Joaquim Coutinho‐Netto, Wagner Ferreira dos Santos, Norberto Cysne Coimbra,
Tópico(s): Neurotransmitter Receptor Influence on Behavior
2004 - Springer Science+Business Media | Cellular and Molecular Neurobiology
Produção Nacional
Revisado por pares
Marcelo Cairrão Araújo Rodrigues, Renato Guizzo, Leonardo Gobbo‐Neto, Richard J. Ward, Norberto Peporine Lopes, Wagner Ferreira dos Santos,
Previous work has shown that the crude venom of Parawixia bistriata induces convulsive seizures in rats after intracerebroventricular injection. In this work, the isolation of a bioactive fraction with ultraviolet absorption characteristics of nucleic acid and trace protein or amino acid content is described. NMR analysis demonstrated that the major component of the active fraction is the nucleoside inosine. An analogue of this component (inosine 5′-monophosphate) induced a delayed paralysis effect ...
Tópico(s): Beetle Biology and Toxicology Studies
2004 - Elsevier BV | Toxicon
Produção Nacional
Revisado por pares
Renê Oliveira Beleboni, Andréa Baldocchi Pizzo, Andréia C. K. Fontana, Ruither de O.G. Carolino, Joaquim Coutinho‐Netto, Wagner Ferreira dos Santos,
Venoms from several arthropods are recognized as useful sources of bioactive substances, such as peptides, acylpolyamines, and alkaloids, which show a wide range of pharmacological effects on synaptic transmission. In this work, we summarize and compile several biochemical and pharmacological aspects related to spider and wasp neurotoxins. Their inhibitory and stimulatory actions on ion channels, receptors, and transporters involved in mammalian and insect neurotransmission are considered.
Tópico(s): Ion channel regulation and function
2004 - Elsevier BV | European Journal of Pharmacology
Produção Nacional
Revisado por pares
Andréa Baldocchi Pizzo, Andr�ia Cristina Karklin Fontana, Joaquim Coutinho‐Netto, Wagner Ferreira dos Santos,
Glutamate (L-glu) is the most important excitatory neurotransmitter in the mammalian central nervous system. Its action is terminated by transporters located in the plasma membrane of neurons and glial cells, which have a critical role in preventing glutamate excitotoxicity under normal conditions. The neurotransmitter gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Venoms of solitary wasps and orb-spiders are composed of large proteins, ...
Tópico(s): Neuroendocrine regulation and behavior
2000 - Wiley | Journal of Biochemical and Molecular Toxicology