Herbert S. Rosenkranz, Elaine B. Winshell, Aiga Mednis, Howard S. Carr, Cornelia J. Ellner,
Hydroxyurea affected neither the synthesis nor the degradation of bacterial messenger-ribonucleic acid. The proteins made by hydroxyurea-treated cells were structurally intact and fully functional. Since the expression of the lethal action of hydroxyurea requires active protein production, the data indicate that treated cells do not die as the result of the synthesis of abnormal proteins.
Tópico(s): Chemical Reaction Mechanisms
1967 - American Society for Microbiology | Journal of Bacteriology
Elaine B. Winshell, Herbert S. Rosenkranz,
Nalidixic acid (NAL) is bactericidal for E. coli B. Synthesis of deoxyribonucleic acid (DNA), ribonucleic acid and protein was necessary to initiate the lethal effect, but only protein synthesis was necessary to sustain it. NAL inhibited DNA synthesis specifically, but this inhibition occurred even under conditions that were not lethal to the bacteria. In contrast to other inhibitors of DNA synthesis, NAL did not cause the solubilization of cellular DNA even when bacteria were exposed to it for 2 ...
Tópico(s): Bacterial Genetics and Biotechnology
1970 - American Society for Microbiology | Journal of Bacteriology
Harold C. Neu, Elaine B. Winshell,
A penicillinase (penicillin β-lactamase) has been purified from a strain of Salmonella typhimurium in which synthesis of the enzyme is mediated by a resistance-transfer factor. The enzyme is located at the surface of the cell and completely released by the cold osmotic shock technique. The penicillinase is not subject to induction by alteration of growth conditions nor is it induced by nonhydrolyzable penicillins. The enzyme can be prepared by DEAE-cellulose and hydroxylapatite column chromatography. ...
Tópico(s): Cancer therapeutics and mechanisms
1970 - Elsevier BV | Archives of Biochemistry and Biophysics
Elaine B. Winshell, William V. Shaw,
Plasmid-mediated chloramphenicol resistance in Staphylococcus aureus has been shown to involve acetylation of chloramphenicol by an enzyme induced by growth in the presence of the antibiotic and certain analogues. Analysis of the kinetics of induction has been complicated by (i) the intrinsic inhibitory effects of chloramphenicol on induced enzyme synthesis and (ii) the rapid disappearance of inducer after synthesis of the acetylating enzyme. The compound related to d -threo chloramphenicol which lacks ...
Tópico(s): Microbial Natural Products and Biosynthesis
1969 - American Society for Microbiology | Journal of Bacteriology
Harold C. Neu, E. B. Winshell,
Tópico(s): Click Chemistry and Applications
1971 - American Society for Microbiology | Applied Microbiology
Harold C. Neu, Elaine B. Winshell,
The activity of 6-[ d (—)-α-carboxy-3-thienylacetamido] penicillanic acid, BRL2288, was determined against Pseudomonas aeruginosa and various gram-negative bacilli. The majority of Pseudomonas strains (89%) were inhibited by 100 μg of the antibiotic per ml. BRL2288 is twofold more active than carbenicillin against Pseudomonas at 100 μg/ml or less. Among Enterobacteriaceae tested, 87% Enterobacter and 87% of Proteus mirabilis strains were inhibited by 25 μg/ml or less. Indole-positive Proteus were inhibited ...
Tópico(s): Phenothiazines and Benzothiazines Synthesis and Activities
1971 - American Society for Microbiology | Applied Microbiology
Harold C. Neu, Elaine B. Winshell,
The Enterobacter species E. aerogenes, E. cloacae , and E. hafnia were examined for resistance to penicillin and cephalosporin derivatives. All were resistant to benzyl penicillin, ampicillin, 6 [ d (−)α-amino- p -hydroxyphenylacetamido] penicillanic acid, cephaloridine, cephalothin, and cephalexin. A significant number were sensitive to carbenicillin and 6 [ d (−)α-carboxy-3-thienylacetamido] penicillanic acid. No differences among the three species were noted. The β-lactamase activity was cell-bound, and was ...
Tópico(s): Antibiotics Pharmacokinetics and Efficacy
1972 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
Tianying An, Jamie Winshell, Gloria Scorzetti, Jack W. Fell, Kathleen S. Rein,
Extracts of fifty-seven newly isolated strains of dinoflagellates and raphidophytes were screened for protein phosphatase (PP2A) inhibition. Five strains, identified by rDNA sequence analysis as Prorocentrum rhathymum, tested positive and the presence of okadaic acid was confirmed in one strain by HPLC-MS/MS and by HPLC with fluorescence detection and HPLC-MS of the okadaic acid ADAM derivative. Quantitation of the ADAM derivative indicated that the concentration of okadaic acid in the culture medium ...
Tópico(s): Microbial Community Ecology and Physiology
2009 - Elsevier BV | Toxicon
Christopher D. Sinigalliano, Jamie Winshell, Maria A. Guerrero, Gloria Scorzetti, Jack W. Fell, R W Eaton, Larry E. Brand, Kathleen S. Rein,
Electronic cell sorting for isolation and culture of dinoflagellates and other marine eukaryotic phytoplankton was compared to the traditional method of manually picking cells using a micropipette. Trauma to electronically sorted cells was not a limiting factor, as fragile dinoflagellates, such as Karenia brevis (Dinophyceae), survived electronic cell sorting to yield viable cells. The rate of successful isolation of large-scale (> 4 litres) cultures was higher for manual picking than for electronic ...
Tópico(s): Marine and coastal ecosystems
2009 - Taylor & Francis | Phycologia
Richard V. Snyder, Maria A. Guerrero, Christopher D. Sinigalliano, Jamie Winshell, Roberto Perez, Jose V. Lopez, Kathleen S. Rein,
Karenia brevis is a toxic marine dinoflagellate endemic to the Gulf of Mexico. Blooms of this harmful alga cause fish kills, marine mammal mortalities and neurotoxic shellfish poisonings. These harmful effects are attributed to a suite of polyketide secondary metabolites known as the brevetoxins. The carbon framework of all polyketides is assembled by a polyketide synthase (PKS). Previously, PKS encoding genes were amplified from K. brevis culture and their similarity to a PKS gene from the closely ...
Tópico(s): Microbial Community Ecology and Physiology
2005 - Elsevier BV | Phytochemistry
Jamie Winshell, James J. Champoux,
Unlike most DNA polymerases, reverse transcriptases can initiate DNA synthesis at a single-strand break and displace the downstream non-template strand simultaneously with extension of the primer. This reaction is important for generation of the long terminal repeat sequences in the duplex DNA product of retroviral reverse transcription. Oligonucleotide-based model displacement constructs were used to study the interaction of human immunodeficiency virus type 1 and Moloney murine leukemia virus ...
Tópico(s): DNA and Nucleic Acid Chemistry
2001 - Elsevier BV | Journal of Molecular Biology
Harold C. Neu, Elaine B. Winshell,
ETHYLENEDIAMINETETBAACETIC acid (EDTA) is known to increase the permeability of Enterobacteriaceae to certain compounds such as actinomycin1 and to make Escherichia coli susceptible to lysozyme lysis2. Presumably, this is related to the disruption of the cell wall characterized by loss of lipopolysaccharide3. Weiser, Asscher and Wim-penny4 reported that EDTA increases the sensitivity of certain organisms resistant to penicillin, ampicillin, chlor-amphenicol and tetracycline presumably by overcoming ...
Tópico(s): Bacterial Genetics and Biotechnology
1970 - Nature Portfolio | Nature
Elaine B. Winshell, Harold C. Neu,
Escherichia coli subjected to cold osmotic shock released 30 to 40% of their fatty acid esters and 42% of their cellular hexosamine. In contrast, Enterobacter , although they released 40% of fatty acid esters, release only 25% of hexosamine. Proteus released less than 15% of either fatty acid esters or hexosamine. These differences are taken to explain the differences among the Enterobacteriaceae in releasing surface enzymes after osmotic shock. It is felt that the release of additional lipopolysaccharide ...
Tópico(s): Microbial bioremediation and biosurfactants
1970 - American Society for Microbiology | Journal of Bacteriology
Harold C. Neu, Elaine B. Winshell,
Cephacetrile, 7-cyanacetamido-cephalosporanic acid, is a new cephalosporin active against many gram-positive and gram-negative organisms. Staphylococcus aureus and Streptococcus pyogenes were inhibited by less than 1 μg/ml, but 25 μg/ml were needed to inhibit enterococci. The majority of Escherichia coli and Klebsiella strains and Proteus mirabilis strains were inhibited by 12.5μg/ml. Serratia, indole-positive Proteus, Pseudomonas and Enterobacter strains were resistant to more than 100 μg/ml. The ...
Tópico(s): Cancer therapeutics and mechanisms
1972 - Springer Nature | The Journal of Antibiotics
Elaine B. Winshell, Harold C. Neu,
Serratia marcescens strains were divided into three groups on the basis of antimicrobial sensitivity and pigment production. Group I, nonpigmented, was resistant to most antibiotics. Group II, nonpigmented, was susceptible to many antimicrobial agents, as was group III which was pigmented. Representative organisms of each group were examined for all lipid content. There were no significant differences in total lipid, phospholipid, or fatty acid esters among the three groups. Differences in susceptibility ...
Tópico(s): Microbial Natural Products and Biosynthesis
1974 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
... A., Anderson, G., Wallace, W., Henley, E. E., Winshell, A. W., and Albert, S. N. (1959): Obstet. ...
Tópico(s): Abdominal Surgery and Complications
1969 - Wiley | BJOG An International Journal of Obstetrics & Gynaecology
Elaine B. Winshell, Robin P. Ertl,
... TO ISSUEPREVArticleNEXTMicro vacuum-distillation of radioactive liquidsElaine B. Winshell and Robin P. ErtlCite this: Anal. Chem. 1986, ...
Tópico(s): Extraction and Separation Processes
1986 - American Chemical Society | Analytical Chemistry
Jamie Winshell, Benjamin A. Paulson, Ben Buelow, James J. Champoux,
DNA displacement synthesis by reverse transcriptase during retroviral replication is required for the production of the linear precursor to integration. The sensitivity of unpaired thymines to KMnO4 oxidation was used to probe for the extent of DNA melting by human immunodeficiency virus, type 1 (HIV-1) reverse transcriptase in front of the primer terminus in model oligonucleotide-based displacement constructs. Unpairing of the two base pairs downstream of the primer (+1 and +2 positions) requires the ...
Tópico(s): DNA and Nucleic Acid Chemistry
2004 - Elsevier BV | Journal of Biological Chemistry
Harold C. Neu, Philip J. Huber, Elaine B. Winshell,
In vivo interbacterial transfer of R factor was observed in two patients. Resistance to ampicillin, tetracycline, streptomycin, and chloramphenicol was transferred from Escherichia coli in the intestine of a patient with shigellosis to a Shigella sonnei strain. In a second patient with partial ureteral obstruction, a resistant E. coli strain transferred ampicillin, tetracycline, and streptomycin resistance to a Proteus mirabilis strain.
Tópico(s): Antibiotic Resistance in Bacteria
1973 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
Pedro Silva, Richard K. Koehn, Walter J. Diehl, Robin P. Ertl, Elaine B. Winshell, Mauro Santos,
Tópico(s): Ion channel regulation and function
1989 - Springer Science+Business Media | Biochemical Genetics
Harold C. Neu, Elaine B. Winshell,
The antibacterial spectrum of cephanone, a 3-heterocyclic thiomethyl cephalosporin derivative, is similar to that of cephalothin and cephalexin. Cephanone is less active against gram-positive organisms but is more active against Escherichia coli than cephalothin. Activity against Klebsiella, Proteus mirabilis and Salmonella is similar to cephalothin. Some Enterobacter strains are ?? susceptible to 8 μg/ml or less. Cephanone is relatively resistant to hydrolysis by gram-negative beta-lactamases. It ...
Tópico(s): Essential Oils and Antimicrobial Activity
1973 - Springer Nature | The Journal of Antibiotics
Tópico(s): Digital Humanities and Scholarship
2014 - | The Journal of The Institute of Image Information and Television Engineers
Tópico(s): Scientific Computing and Data Management
2013 - | The Journal of The Institute of Image Information and Television Engineers
... the data from which was used for a WinShell mass balance model based on an individual Med ...
Tópico(s): Marine and fisheries research
2023 - National Shellfisheries Association | Journal of Shellfish Research
James A. Kaufman, Elaine Winshell,
... author on: This Site PubMed Google Scholar Elaine Winshell Elaine Winshell Search for other works by this author on: ... Cite Search Site Citation James A. Kaufman, Elaine Winshell; Letters to the Editor. The American Biology Teacher ...
Tópico(s): Veterinary Practice and Education Studies
1990 - University of California Press | The American Biology Teacher
Pedro Silva, Richard K. Koehn, Walter J. Diehl, Robin P. Ertl, Elaine B. Winshell, Mauro Santos,
Tópico(s): Erythrocyte Function and Pathophysiology
1989 - Springer Science+Business Media | Biochemical Genetics