Y. Kato, Masakazu Yashiro, Satoru Noda, Masashige Tendo, Shinichiro Kashiwagi, Yosuke Doi, Takafumi Nishii, Junko Matsuoka, Y Fuyuhiro, Osamu Shinto, Takeshi Sawada, Masahiro Ohira, Kosei Hirakawa,
Many kinds of solid tumour have heterogeneously a hypoxic environment. Tumour hypoxia reported to be associated with more aggressive tumour phenotypes such as high metastatic ability and resistance to various anti-cancer therapies which may lead to a poorer prognosis. However, the mechanisms by which hypoxia affects the aggressive phenotypes remain unclear.We established a scirrhous gastric carcinoma cell line (OCUM-12) from ascites associated with scirrhous gastric carcinoma, and a hypoxia-resistant ...
Tópico(s): Cancer-related Molecular Pathways
2010 - Springer Nature | British Journal of Cancer
Kensaku Nagasawa, Taiki Ishida, Hayato Kashiwagi, Yosuke Sano, Shigenori Mitsushima,
In this study, we designed and developed a compact electrolyzer for the evaluation of components in proton exchange membrane (PEM) water electrolysis. First, this electrolyzer features a precise pressure-control system that controls the active electrode area and facilitates setting the desired clamping pressure. This mechanism makes it possible to optimize the electrolyzer performance. Second, it has two reference electrodes that are connected on the faces of the active electrode area of the anode ...
Tópico(s): Advanced Battery Technologies Research
2021 - Elsevier BV | International Journal of Hydrogen Energy
Hiroyuki Imaeda, Naoki Hosoe, Kazuhiro Kashiwagi, Yosuke Ida∥, Rieko Nakamura, Hidekazu Suzuki, Yoshimasa Saito, Naohisa Yahagi, Yasushi Iwao, Yuko Kitagawa, Toshifumi Hibi∥, Haruhiko Ogata, Takanori Kanai∥,
AIM:To evaluate the effectiveness of trimodal imaging endoscopy (TME) to detect another lesion after endoscopic submucosal dissection (ESD) for superficial gastric neoplasia (SGN). METHODS:Surveillance esophagogastroduodenoscopy (EGD) using a TME was conducted in 182 patients that had undergone ESD for SGN.Autofluorescence imaging (AFI) was conducted after white-light imaging (WLI).When SGN was suspicious, magnifying endoscopy with narrow-band imaging (ME-NBI) was conducted.Final diagnoses were made by ...
Tópico(s): Esophageal Cancer Research and Treatment
2014 - Baishideng Publishing Group | World Journal of Gastroenterology
Tsukasa Kokubo, Tatsuya Mori, Takashi Kurokawa, Ken Kashiwagi, Yosuke Tanaka, Takayuki Kotani, Jun Nishikawa, Motohide Tamura,
In order to detect Earth-like planets around nearby red dwarfs (in particular late-M stars), it is crucial to conduct precise radial velocity measurements at near-infrared wavelengths where these stars emit most of the light. We have been developing the Infrared Doppler (IRD) spectrograph which is a high dispersion spectrograph for the Subaru telescope. To achieve 1m/s RV measurement precision, we have developed a direct generation of laser frequency comb (LFC) that uses high-repetition-rate pump pulse ...
Tópico(s): Advanced Fiber Optic Sensors
2016 - SPIE | Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE
Nam‐ho Huh, Mariko Kashiwagi, Chieko Konishi, Yosuke Hashimoto, Yohko Kohno, Toshio Kuroki, Shintaro Nomura,
We have isolated a hair-follicle-specific gene, termed hacl-1, from a cDNA library of ICR mouse skin. Hacl-1 is expressed specifically in skin, and its mRNA level is correlated with the active state of hair follicles in developmental and regenerative processes of hair. Its mRNA is approximately 1 kilobase pairs (kb). Its cDNA was completely co-linear with the genomic clone, indicating that the hacl-1 gene is composed of one exon. The hacl-1 gene has an open reading frame of 579 base pairs (bp). The ...
Tópico(s): Wnt/β-catenin signaling in development and cancer
1994 - Elsevier BV | Journal of Investigative Dermatology
Yuhiko Fuyuhiro, Masakazu Yashiro, Satoru Noda, Shinichiro Kashiwagi, Junko Matsuoka, Yosuke Doi, Y. Kato, Kazuya Muguruma, Tetsuji Sawada, Kosei Hirakawa,
Fibroblasts, particularly myofibroblasts, affect the malignant progression of cancer cells in vitro. However, to date few reports have addressed the clinical significance of myofibroblasts in the gastric cancer microenvironment. This study examined the correlation between myofibroblast expression and clinicopathological features in gastric carcinoma. A total of 265 primary gastric tumors resected by gastrectomy were stained with antibodies against α-smooth muscle actin and vimentin. Stromal cells ...
Tópico(s): Cancer Cells and Metastasis
2010 - Spandidos Publishing | Experimental and Therapeutic Medicine
Samuel Choi, Naoyuki Tamura, Ken Kashiwagi, Tatsutoshi Shioda, Yosuke Tanaka, Takashi Kurokawa,
We propose a novel supercontinuum (SC) generation technology using an optical pulse synthesizer and highly nonlinear dispersion-shifted fiber. The optical pulse synthesizer enables us to reshape seed pulses optimized for broadband SC generation. 25.0 and 12.5 GHz SC combs were successfully generated by propagating synthesized seed pulses in the highly nonlinear fiber. We investigated how seed pulse conditions including shape, peak power, repetition rate, and center wavelength affect SC spectral bandwidth. ...
Tópico(s): Advanced Fiber Optic Sensors
2009 - Institute of Physics | Japanese Journal of Applied Physics
Y Fuyuhiro, Masakazu Yashiro, Satoru Noda, Shinichiro Kashiwagi, Junko Matsuoka, Yosuke Doi, Y. Kato, Tsuyoshi Hasegawa, Takeshi Sawada, Kosei Hirakawa,
Myofibroblasts in the cancer microenvironment have recently been implicated in tumour growth and metastasis of gastric cancer. However, the mechanisms responsible for the regulation of myofibroblasts in cancer-associated fibroblasts (CAFs) remain unclear. This study was performed to clarify the mechanisms for regulation of myofibroblasts in gastric cancer microenvironment. Two CAFs (CaF-29 and CaF-33) from the tumoural gastric wall and a normal fibroblast (NF-29) from the nontumoural gastric wall, ...
Tópico(s): Cancer Cells and Metastasis
2011 - Springer Nature | British Journal of Cancer
K Ishibashi, T Kashiwagi, Akio Ito, Yosuke Tanaka, M Nagasawa, Tetsuya Toyama, S. Ozaki, Masafumi Naito, M Azuma,
We serially measured the levels of serum N-terminal peptide of type III procollagen (PIIINP), the 7S domain of type IV collagen (IV-7S), and hyaluronate (HA) before (0 month), at the end (6 months), and 24 weeks after the end of interferon therapy (12 months) in patients with chronic hepatitis type C to estimate the effects of interferon alfa (IFN-alpha) on serum levels of hepatic fibrogenesis markers. One hundred twenty-one patients with chronic hepatitis type C received intramuscular injection of 6 million ...
Tópico(s): Liver Diseases and Immunity
1996 - Lippincott Williams & Wilkins | Hepatology
Hiroyuki Imaeda, Naoki Hosoe, Kazuhiro Kashiwagi, Yosuke Ida∥, Yoshimasa Saito, Hidekazu Suzuki, Koichi Aiura, Haruhiko Ogata, Koichiro Kumai, Toshifumi Hibi∥,
Abstract Background: Autofluorescence (AF) videoendoscopy has an advantage over ordinary videoendoscopy in the diagnosis of gastric neoplasias, and the aim of the present study was to evaluate the effectiveness of using the SAFE‐3000 videoendoscopy system to diagnose superficial gastric neoplasias. Methods: Ordinary videoendoscopy, AF videoendoscopy, and chromoendoscopy (CE) were used to diagnose the tumor existence and extent in 14 patients with gastric adenoma, 40 patients with intestinal‐type early ...
Tópico(s): Esophageal Cancer Research and Treatment
2009 - Wiley | Journal of Gastroenterology and Hepatology
Y. Kato, Masakazu Yashiro, Satoru Noda, Shinichiro Kashiwagi, Junko Matsuoka, Yuhiko Fuyuhiro, Yosuke Doi, Kosei Hirakawa,
<i>Background/Aims:</i> Gastric cancers are characterized by a heterogeneously hypoxic environment. Hypoxia might stimulate the malignant potential of cancer cells. The purpose of our study was to clarify the significance of hypoxia in gastric carcinoma by evaluating the expression of a hypoxic marker, namely carbonic anhydrase-9 (CA-9). <i>Methods:</i> A total of 265 patients who had undergone a resection of the primary tumor and were confirmed histologically to have sporadic ...
Tópico(s): Cancer-related gene regulation
2010 - Karger Publishers | Digestion
Hiroyuki Imaeda, Naoki Hosoe, Yosuke Ida∥, Kazuhiro Kashiwagi, Yuichi Morohoshi, Kazuhiro Suganuma, Shuichi Nagakubo, Koichi Komatsu, Hidekazu Suzuki, Yoshimasa Saito, Koichi Aiura, Haruhiko Ogata, Yasushi Iwao, Koichiro Kumai, Yuko Kitagawa, Toshifumi Hibi∥,
Endoscopic submucosal dissection (ESD) for early stage gastric cancer (EGC) has improved the success rate of en bloc resection but results in perforation more often than does endoscopic mucosal resection. We report a novel technique of ESD using an external grasping forceps. A total of 265 lesions with EGC or gastric adenoma were enrolled in this study. Sixteen lesions were located in the upper third portion of the stomach, 114 in the middle third portion, and 135 in the lower third portion. After ...
Tópico(s): Gastrointestinal Tumor Research and Treatment
2009 - Wiley | Digestive Endoscopy
Ken Kashiwagi, Takashi Kurokawa, Yasushi Okuyama, Takahiro Mori, Yosuke Tanaka, Yoshinori Yamamoto, Masaaki Hirano,
We generated a 12.5-GHz-spacing optical frequency comb that can be resolved over 100 THz, from 1040 to 1750 nm, without spectral mode filtering. To cover such a broad spectrum, we used electro-optic modulation of single frequency light and line-by-line pulse synthesis to produce a clear pulse train and subsequent spectral broadening in highly nonlinear fibers (HNLFs). We numerically and experimentally investigated a configuration of the HNLFs and find that a two-stage broadening through different ...
Tópico(s): Advanced Fiber Optic Sensors
2016 - Optica Publishing Group | Optics Express
Manabu Kashiwagi, Yuichi Ozaki, Toshio Imanishi, Akira Taruya, Akio Kuroi, Yosuke Katayama, Kunihiro Shimamura, Yasutsugu Shiono, Takashi Tanimoto, Takashi Kubo, Atsushi Tanaka, Takashi Akasaka,
Kashiwagi, Manabu; Ozaki, Yuichi; Imanishi, Toshio; Taruya, Akira; Kuroi, Akio; Katayama, Yosuke; Shimamura, Kunihiro; Shiono, Yasutsugu; Tanimoto, Takashi; Kubo, Takashi; Tanaka, Atsushi; Akasaka, Takashi Author Information
Tópico(s): Neutrophil, Myeloperoxidase and Oxidative Mechanisms
2021 - Lippincott Williams & Wilkins | Coronary Artery Disease
Weifan Qiao, Kiyonobu Mozawa, Ken Kashiwagi, Yosuke Tanaka, Takashi Kurokawa,
To suppress nonlinear effects in optical fibers, we propose phase only pulses (POPs) that exhibit constant amplitude and pulse-shaped phase waveforms and investigate a method of generating these pulses. POPs with two phase waveforms, i.e., sech2- and Gaussian-shaped phase waveforms, are synthesized using an optical pulse synthesizer (OPS). Feedback control of the OPS allows for adjustment of the phase spectra and realization of temporally constant amplitudes and pulse-shaped phase waveforms.
Tópico(s): Advanced Photonic Communication Systems
2011 - Institute of Physics | Applied Physics Express
Hiroyuki Imaeda, Naoki Hosoe, Yosuke Ida∥, Hiromasa Nakamizo, Kazuhiro Kashiwagi, Takanori Kanai∥, Yasushi Iwao, Toshifumi Hibi∥, Haruhiko Ogata,
Endoscopic submucosal dissection (ESD) is more difficult for rectal cancer than for gastric cancer.To evaluate the feasibility and safety of an ESD procedure by using external forceps for early rectal cancer.A case series.A tertiary medical center.Thirteen patients with early-stage rectal cancer were enrolled. Twelve of the 13 lesions were granular-type laterally spreading tumors and 1 was a protruding tumor.After circumferential incision around the lesion with a dual-knife or a flex-knife, bendable ...
Tópico(s): Gastrointestinal Tumor Research and Treatment
2012 - Elsevier BV | Gastrointestinal Endoscopy
Atsushi Tanaka, Akira Taruya, K Shibata, K Fuse, Yosuke Katayama, Mao Yokoyama, Manabu Kashiwagi, Shingo Ota, Takashi Akasaka, Nobuhiro Kato,
Abstract Refractory angina is an independent predictor of adverse events in patients with vasospastic angina (VSA). The aim of this study was to investigate the relationship between coronary lumen complexity and refractory symptoms in patients with VSA. Seventeen patients with VSA underwent optical coherence tomography. The patients were divided into the refractory VSA group (n = 9) and the stable VSA group (n = 8). A shoreline development index was used to assess the coronary artery lumen complexity. ...
Tópico(s): Cardiovascular Health and Disease Prevention
2021 - Nature Portfolio | Scientific Reports
Yosuke Katayama, Akira Taruya, Manabu Kashiwagi, Yuichi Ozaki, Yasutsugu Shiono, Takashi Tanimoto, Takanori Yoshikawa, Toshikazu Kondo, Atsushi Tanaka,
Tópico(s): Coronary Interventions and Diagnostics
2022 - Elsevier BV | IJC Heart & Vasculature
Takuya Yoshino, Tomohisa Nagoshi, Ryuko Anzawa, Yusuke Kashiwagi, Keiichi Ito, Daisuke Katoh, Masami Fujisaki, Yosuke Kayama, Taro Date, Kenichi Hongo, Michihiro Yoshimura,
Although persistent excessive actions of aldosterone have unfavorable effects on the cardiovascular system, primarily via mineralocorticoid receptor (MR)-dependent pathways, the pathophysiological significance of aldosterone cascade activation in heart diseases has not yet been fully clarified. We herein examined the effects of short-term aldosterone stimulation at a physiological dose on cardiac function during ischemia–reperfusion injury (IRI). In order to study the effects of aldosterone preconditioning, ...
Tópico(s): Electrolyte and hormonal disorders
2014 - Bioscientifica | Journal of Endocrinology
Samuel Choi, Ken Kashiwagi, Shuto Kojima, Yosuke Kasuya, Takashi Kurokawa,
The multi-gigahertz frequency comb-based interferometer exhibits only the interference amplitude peak without the phase fringes, which can produce a rapid axial scan for full-field profilometry and tomography. Despite huge technical advantages, there remain problems that the interference intensity undulations occurred depending on the interference phase. To avoid such problems, we propose a compensation technique of the interference signals using two frequency combs with slightly varied center wavelengths. ...
Tópico(s): Optical measurement and interference techniques
2013 - Institute of Physics | Applied Physics Express
Ken Kashiwagi, Yuichiro Kodama, Ryo Kobe, Tatsutoshi Shioda, Yosuke Tanaka, Takashi Kurokawa,
In this study, we experimentally characterize optical short-pulse transmission through single-mode fibers. The initial pulses were precisely generated by an optical pulse synthesizer. The optical pulse synthesizer provides line-by-line phase and intensity modulations to individual channels in optical frequency combs and generates precise arbitral pulses. Pulse shaping enables the experimental evaluation of pulse transmission along the fibers under various initial pulse conditions, and the pulse ...
Tópico(s): Advanced Photonic Communication Systems
2009 - Institute of Physics | Japanese Journal of Applied Physics
Yuma Hotta, Kazuhiko Uchiyama, Tomohisa Takagi, Saori Kashiwagi, Takahiro Nakano, Rieko Mukai, Yuki Toyokawa, Tomoyo Yasuda, Tomohiro Ueda, Yosuke Suyama, Takaaki Murakami, Makoto Tanaka, Atsushi Majima, Toshifumi Doi, Yasuko Hirai, Katsura Mizushima, Mayuko Morita, Yasuki Higashimura, Ken Inoue, Akifumi Fukui, Tetsuya Okayama, Kazuhiro Katada, Kazuhiro Kamada, Osamu Handa, Takeshi Ishikawa, Yuji Naito, Yoshito Itoh,
Intestinal fibrosis with stricture formation is a severe complication of Crohn's disease (CD). Though new therapeutic targets to enable the prevention or treatment of intestinal fibrosis are needed, markers of this condition and the basic mechanisms responsible have not been established. NADPH oxidase (NOX) 4 has already been reported to play a key role in models of fibrogenesis, including that of the lung. However, its importance in intestinal fibrogenesis remains unclear. In this study, we examined ...
Tópico(s): IL-33, ST2, and ILC Pathways
2018 - Elsevier BV | Biochemical and Biophysical Research Communications
Tadamitsu Kishimoto, Yoshifumi Kubota, Tomohiro Matsushima, Hiroshi Izutsu, Akio Matsumoto, R Soejima, Kei Numazaki, S. Chiba, Tsutomu Yamazaki, Naoko Sasaki, M Kaku, Junko Shimada, E Iwasaki, M. Baba, Y Koori, Masanori Aihara, Hiroki Chikumi, S Kosaba, Yosuke Nonaka, Kazunobu Ouchi, Takuya Yamamoto, S Kashiwagi, T. Kawayama, Kotaro Ohizumi, Hisaki Nagai,
We measured anti-Chlamydia pneumoniae (C. pneumoniae) specific antibody titers by means of a newly-developed enzyme-linked immunosorbent assay (ELISA) method using an anti-C. pneumoniae specific antibody detection reagent. The clinical usefulness of this method was hereby evaluated. The IgG, IgA and IgM titers in 418 serum specimens obtained from patients with respiratory tract infections were measured by this new ELISA method, and the results were compared with the titers determined for the same ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
1996 - | Kansenshogaku zasshi
Yosuke Katayama, Atsushi Tanaka, Akira Taruya, Manabu Kashiwagi, Tsuyoshi Nishiguchi, Yuichi Ozaki, Yoshiki Matsuo, Hironori Kitabata, Takashi Kubo, Emi Shimada, Toshikazu Kondo, Takashi Akasaka,
Cholesterol crystals (CCs) are frequently found at the site of acute myocardial infarctions (AMIs), but the role of CCs in the onset of AMI remains unclear due to the lack of validated in vivo imaging tools. The aim of this study was to validate the ability of optical coherence tomography (OCT) to detect CCs and to compare the prevalence and distribution of CCs in patients with AMIs and stable angina pectoris. Approach and Results: CC assessment using OCT were compared with histopathology results in ...
Tópico(s): Systemic Lupus Erythematosus Research
2019 - Lippincott Williams & Wilkins | Arteriosclerosis Thrombosis and Vascular Biology
Yosuke Katayama, Atsushi Tanaka, Akira Taruya, Manabu Kashiwagi, Tsuyoshi Nishiguchi, Yuichi Ozaki, Yasutsugu Shiono, Kunihiro Shimamura, Hironori Kitabata, Takashi Kubo, Takeshi Hozumi, Yuko Ishida, Toshikazu Kondo, Takashi Akasaka,
Background It has been widely documented that circannual variation has an impact on the incidence and prognosis of cardiovascular diseases. It is unclear why cold ambient temperature increase the incidence of acute myocardial infarction (AMI). We investigated the relationship between the ambient temperature at the onset of AMI, the morphology of the culprit lesion in patients with AMI. Methods We investigated 202 consecutive patients with AMI who underwent optical coherence tomography (OCT). The participants ...
Tópico(s): Health Systems, Economic Evaluations, Quality of Life
2020 - Elsevier BV | International Journal of Cardiology
Azusa Hasegawa, Ken Kashiwagi, Yosuke Tanaka, Takashi Kurokawa,
In this paper, we investigated the imaging condition of asymmetric pulses on a temporal imaging (TI) system comprising two optical fibers and a phase modulator. We introduced a fitness function to evaluate the difference between target and output waveforms to quantitatively estimate TI characteristics. We successfully determined the optimal combination of phase modulation index and phase deviation to provide good TI by simulating the fitness function. We generated chirp-free pulses with symmetric ...
Tópico(s): Optical Network Technologies
2015 - Institute of Electrical and Electronics Engineers | IEEE photonics journal
M. Tamura, Hitoshi Suto, Jun Nishikawa, Takayuki Kotani, Bun’ei Sato, Wako Aoki, Tomonori Usuda, Takashi Kurokawa, Ken Kashiwagi, Shogo Nishiyama, Yuji Ikeda, D. Hall, K. W. Hodapp, Jun Hashimoto, Jun‐Ichi Morino, Sadahiro Inoue, Yosuke Mizuno, Yo Washizaki, Yosuke Tanaka, S. Suzuki, Jungmi Kwon, Takuya Suenaga, Dehyun Oh, Norio Narita, Eiichiro Kokubo, Yutaka Hayano, Hideyuki Izumiura, Eiji Kambe, Tomoyuki Kudo, Nobuhiko Kusakabe, Masahiro Ikoma, Yasunori Hori, Masashi Omiya, Hidenori Genda, Akihiko Fukui, Yuka Fujii, Olivier Guyon, Hiroki Harakawa, Masahiko Hayashi, M. Hidai, Teruyuki Hirano, Masayuki Kuzuhara, Masahiro N. Machida, Taro Matsuo, Takako Nagata, Hiroshi Ohnuki, Masahiro Ogihara, Shoichi Oshino, Ryuji Suzuki, Hideki Takami, N. Takato, Yuji Takahashi, Chihiro Tachinami, Hiroshi Terada,
IRD is the near-infrared high-precision radial velocity instrument for the Subaru 8.2-m telescope. It is a relatively compact (~1m size) spectrometer with a new echelle-grating and Volume-Phase Holographic gratings covering 1-2 micron wavelengths combined with an original frequency comb using optical pulse synthesizer. The spectrometer will employ a 4096x4096-pixel HgCdTe array under testing at IfA, University of Hawaii. Both the telescope/Adaptive Optics and comb beams are fed to the spectrometer ...
Tópico(s): Astrophysics and Star Formation Studies
2012 - SPIE | Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE
Jun Arima, Kohei Taniguchi, Masashi Yamamoto, Tsubasa Watanabe, Yusuke Suzuki, Hiroki Hamamoto, Yosuke Inomata, Hideki Kashiwagi, Shinji Kawabata, Keitaro Tanaka, Kazuhisa Uchiyama, Minoru Suzuki, Sang-Woong Lee,
Local recurrence of colorectal cancer (CRC) can occur in patients after curative resection, and additional surgical resection may therefore be required; however, this is a significant burden for patients, because additional surgical resection may necessitate the resection of other organs such as the bladder, prostate, uterus, or sacral bone. Therefore, there is a need for alternative therapeutic strategies. We focused on boron neutron capture therapy (BNCT) as a treatment modality that can selectively ...
Tópico(s): Nuclear Physics and Applications
2022 - Elsevier BV | Biomedicine & Pharmacotherapy
Ken Kashiwagi, Kiyonobu Mozawa, Yosuke Tanaka, Takashi Kurokawa,
We precisely generate dark solitons using an optical pulse synthesizer (OPS) at a repetition rate of 25 GHz and experimentally investigate soliton transmission through a normal-dispersion fiber. Because of their particular waveform, there are not many experimental studies. The OPS provides frequency-domain line-by-line modulation and produces arbitrary pulse waveforms. The soliton waveform has an intensity contrast greater than 20 dB. At certain input peak power, the pulse exhibits soliton transmission ...
Tópico(s): Photonic and Optical Devices
2013 - Optica Publishing Group | Optics Express
Shinsuke Murakami, Jun Nakatani, Kenichi Nakajima, Eri Amasawa, Ryota Ii, Kiyotada Hayashi, Naoki Yoshikawa, Ichiro Daigo, Yusuke Kishita, Tomohiko Ihara, Koichi Shobatake, Y. Kudoh, Masaharu Motoshita, Keiichiro Kanemoto, Minako Hara, Aiichiro Kashiwagi, Seiji Hashimoto, Yosuke Shigetomi, Masayuki Kanzaki, Yasunori Kikuchi, Hajime Ohno, Yasuhiro Fukushima,
Tópico(s): Sustainable Industrial Ecology
2019 - Springer Science+Business Media | The International Journal of Life Cycle Assessment