Te Bi, Yu-Hao Chang, Wenxi Fei, Masayuki Iwataki, Aoi Morishita, Yu Fu, Naoya Niikura, Hiroshi Kawarada,
A C–Si bonded SiO2/diamond interface is formed under a SiO2 mask during the selective diamond growth at a high temperature in a H2 atmosphere including methane (5%). A few monolayers with C–Si bonding at the SiO2/diamond surface are confirmed through X-ray photoelectron spectroscopy at the C1s and Si2p core levels from 290 eV to 271 eV and 107 eV–95 eV, respectively. In addition, secondary ion mass spectroscopy results suggest that the C–Si bonds, and not C–H bonds, are majority at the interface ...
Tópico(s): Semiconductor materials and devices
2021 - Elsevier BV | Carbon
Yui Umehara, Toki Kageyama, Aoi Son, Yu Kimura, Teruyuki Kondo, Kazuhito Tanabe,
Tumor-selective accumulation of gold nanorods (GNR) has been demonstrated for visualization of tumor hypoxia by photoacoustic imaging. We prepared GNRs with hypoxia-targeting nitroimidazole units (G-NI) on their surface. Biological experiments revealed that G-NI produced a strong photoacoustic signal in hypoxic tumor cells and tissues.
Tópico(s): Extracellular vesicles in disease
2019 - Royal Society of Chemistry | RSC Advances
Aoi Isono, Mieko Tsuji, Yu Sanada, Akari Matsushita, Shin‐ichiro Masunaga, Tasuku Hirayama, Hideko Nagasawa,
Abstract We developed new 10 B carriers for boron neutron capture therapy (BNCT) that can effectively transport and accumulate boron clusters into cells. These carriers consist of a lipopeptide, mercaptoundecahydrododecaborate (BSH), and a disulfide linker. The carriers were conceived according to the structure of pepducin, a membrane‐penetrating lipopeptide targeting protease‐activated receptor 1 (PAR1). To improve the membrane permeability of BSH, the structure was optimized using various lipopeptides ...
Tópico(s): Radiopharmaceutical Chemistry and Applications
2019 - Wiley | ChemMedChem
Aoi Ogawa, Junpei Dake, Yu-ki Iwashina, Toshinobu Tokumoto,
Abstract Background The African clawed frog, Xenopus laevis , is widely used in studies of oogenesis, meiotic cell cycle and early embryonic development. However, in order to perform such studies, eggs are normally collected after the injection of hCG into the dorsal lymph sac of fully-grown female frogs following pre-injection of PMSF. Although this protocol is established and used as standard laboratory approach, there are some concerns over whether the injections could cause the transmission of ...
Tópico(s): Reproductive Biology and Fertility
2011 - BioMed Central | Reproductive Biology and Endocrinology
Shin-Ichi Mae, Fumihiko Hattanda, Hiroyoshi Morita, Aya Nozaki, Naoko Katagiri, Hanako Ogawa, Kaori Teranaka, Yu Nishimura, Aoi Kudoh, Sanae Yamanaka, Kyoko Matsuse, Makoto Ryosaka, Akira Watanabe, Tomoyoshi Soga, Saori Nishio, Kenji Osafune,
In autosomal dominant polycystic kidney disease (ADPKD), renal cyst lesions predominantly arise from collecting ducts (CDs). However, relevant CD cyst models using human cells are lacking. Although previous reports have generated in vitro renal tubule cyst models from human induced pluripotent stem cells (hiPSCs), therapeutic drug candidates for ADPKD have not been identified. Here, by establishing expansion cultures of hiPSC-derived ureteric bud tip cells, an embryonic precursor that gives rise to ...
Tópico(s): Renal cell carcinoma treatment
2023 - Cell Press | Cell Reports
Immunohistochemical method to detect avian lymphocytes is an efficient and reliable tool for accurate diagnosis, and immunological analysis of avian diseases. However, there are scarce studies reporting immunohistochemistry (IHC) using commercially available antibodies against lymphocytes in formalin-fixed paraffin-embedded (FFPE) chicken tissues. In the present study, we established an immunohistochemical method to identify chicken T and B lymphocytes in FFPE chicken tissues using commercial antibodies ...
Tópico(s): Animal Virus Infections Studies
2020 - Elsevier BV | Veterinary Immunology and Immunopathology
Yu Sugihara, Aoi Kudoh, Muluneh Tamiru Oli, Hiroki Takagi, Satoshi Natsume, Motoki Shimizu, Akira Abe, Robert Asiedu, Asrat Asfaw, Patrick Adebola, Ryohei Terauchi,
Abstract Yam is a collective name of tuber crops belonging to the genus Dioscorea . Yam is important not only as a staple food crop but also as an integral component of society and culture of the millions of people who depend on it. However, due to its regional importance, yam has long been regarded as an “orphan crop” lacking a due global attention. Although this perception is changing with recent advances in genomics technologies, domestication processes of most yam species are still ambiguous. This ...
Tópico(s): Banana Cultivation and Research
2021 - Springer International Publishing | Population genomics
Hirokazu Komatsu, Kazuki Yoshihara, H. Yamada, Yu Kimura, Aoi Son, Sei‐ichi Nishimoto, Kazuhito Tanabe,
Abstract The phosphorescence emission of ruthenium complexes was applied to the optical imaging of physiological hypoxia. We prepared three complexes with hydrophobic substituents on the phenanthroline ligand and characterized their emission, which was quenched by molecular oxygen. Among the complexes synthesized in this study, a pyrene chromophore‐linked ruthenium complex, Ru‐Py, exhibited optimal properties for the imaging of hypoxia; the prolonged lifetime of the triplet excited state of the ...
Tópico(s): Molecular Sensors and Ion Detection
2012 - Wiley | Chemistry - A European Journal
Aoi Ohira, Sayaka Yamaguchi, Takuya Miyagi, Yu-ichi YAMAMOTO, Satoshi Yamada, Hideo Shiohira, Keisuke Hagiwara, Tsukasa Uno, Hiroshi Uezato, Kenzo Takahashi,
Abstract Fixed drug eruption is a common cutaneous adverse reaction in young patients with a characteristic clinical appearance. However, the diagnosis and identification of the substance may be difficult if food or food additives provoke the fixed eruption. A 26‐year‐old man had a history of two episodes of cutaneous erythema with residual pigmentation. Close examination of the history including his diet in addition to an oral challenge test and patch testing led to the diagnosis of fixed eruption ...
Tópico(s): Food Allergy and Anaphylaxis Research
2013 - Wiley | The Journal of Dermatology
Marek disease (MD) is a viral disease characterized by the development of lymphoma in poultry. Although morphologic confirmation of lymphoma is used to diagnose MD, immunohistochemical detection of MD virus-EcoRI-Q (Meq), which is a viral protein that is expressed exclusively in MD tumor cells, would further improve the accuracy of diagnosis. We developed monoclonal antibodies (mAbs) that specifically detect Meq by immunohistochemistry (IHC) using formalin-fixed, paraffin-embedded (FFPE) sections. We ...
Tópico(s): Virus-based gene therapy research
2022 - SAGE Publishing | Journal of Veterinary Diagnostic Investigation
Kumiko Hosokawa, Kunitoshi Imai, Hieu Van Dong, Haruko Ogawa, Madoka SUZUTOU, Sandi Htein LINN, Aoi Kurokawa, Yu Yamamoto,
A concurrent infection of chicken anemia virus (CAV) and infectious bronchitis virus (IBV) was detected in Japanese native chicks in 2017, in which a high mortality rate (97.7%) was recorded in a small flock of 130 chicks exhibiting poor growth. Histological examination revealed that the affected chicks exhibited two different pathological entities: one was severe hematopoietic and lymphocytic depletion with abnormally large cells containing intranuclear inclusion bodies of CAV, whereas the other was ...
Tópico(s): Viral gastroenteritis research and epidemiology
2020 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Teruyuki Kondo, Tsutomu Yoshimura, Shun-ichi Naito, Di Yuanjun, Son Aoi, Yu Kimura, Akio Toshimitsu,
Tópico(s): Phenothiazines and Benzothiazines Synthesis and Activities
2016 - Elsevier BV | Heterocycles
Gary LeRoy, Ozgur Oksuz, Nicolas Descostes, Yuki Aoi, Rais A. Ganai, Havva Ortabozkoyun, Jia-Ray Yu, Chul‐Hwan Lee, James M. Stafford, Ali Shilatifard, Danny Reinberg,
Chromatin-bound LEDGF and HDGF2 proteins allow RNAPII to overcome the nucleosome-induced block to transcription.
Tópico(s): Epigenetics and DNA Methylation
2019 - American Association for the Advancement of Science | Science Advances
Aoi Hosaka, Raku Saito, Kazuya Takashima, Taku Sasaki, Yu Fu, Akira Kawabe, Tasuku Ito, Atsushi Toyoda, Asao Fujiyama, Yoshiaki Tarutani, Tetsuji Kakutani,
The arms race between parasitic sequences and their hosts is a major driving force for evolution of gene control systems. Since transposable elements (TEs) are potentially deleterious, eukaryotes silence them by epigenetic mechanisms such as DNA methylation. Little is known about how TEs counteract silencing to propagate during evolution. Here, we report behavior of sequence-specific anti-silencing proteins used by Arabidopsis TEs and evolution of those proteins and their target sequences. We show ...
Tópico(s): Plant Molecular Biology Research
2017 - Nature Portfolio | Nature Communications
Jinghe Li, Ryota Inoue, Yu Togashi, Tomoko Okuyama, Aoi Satoh, Mayu Kyohara, Kuniyuki Nishiyama, Takahiro Tsuno, Daisuke Miyashita, Tatsuya Kin, A. M. James Shapiro, Resilind Su Ern Chew, Adrian Kee Keong Teo, Seiichi Oyadomari, Yasuo Terauchi, Jun Shirakawa,
The effects of imeglimin, a novel antidiabetes agent, on β-cell function remain unclear. Here, we unveiled the impact of imeglimin on β-cell survival. Treatment with imeglimin augmented mitochondrial function, enhanced insulin secretion, promoted β-cell proliferation, and improved β-cell survival in mouse islets. Imeglimin upregulated the expression of endoplasmic reticulum (ER)-related molecules, including Chop (Ddit3), Gadd34 (Ppp1r15a), Atf3, and Sdf2l1, and decreased eIF2α phosphorylation after ...
Tópico(s): Calcium signaling and nucleotide metabolism
2021 - American Diabetes Association | Diabetes
Providing a method to detect avian lymphocytes by immunohistochemistry (IHC) would be helpful for analyzing immune function and diagnosing diseases in birds. In this study, we comprehensively examined the immunohistochemical identification of avian T and B lymphocytes in formalin-fixed, paraffin-embedded tissues from 53 avian species across 15 orders, using eight commercially available lymphocyte markers. T lymphocytes from all 53 avian species tested were specifically detected by IHC using the anti- ...
Tópico(s): T-cell and B-cell Immunology
2023 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Koichiro Watanabe, Yasuaki Ikuno, Yumi Kakeya, Hirotaka Kito, Aoi Matsubara, Mizuki Kaneda, Yu Katsuyama, Hayato Naka-Kaneda,
Restoration of tissue homeostasis by controlling stem cell aging is a promising therapeutic approach for geriatric disorders. The molecular mechanisms underlying age-related dysfunctions of specific types of adult tissue stem cells (TSCs) have been studied, and various microRNAs were recently reported to be involved. However, the central roles of microRNAs in stem cell aging remain unclear. Interest in this area was sparked by murine heterochronic parabiosis experiments, which demonstrated that systemic ...
Tópico(s): Extracellular vesicles in disease
2018 - BioMed Central | Inflammation and Regeneration
Seiya Ichihara, Hirokazu Ogino, Hiroto Yoneda, Keiko Haji, Kozo Kagawa, Kojin Murakami, Masato Mima, Yu Aoi, Atsushi Mitsuhashi, Yuki Tsukazaki, Yohei Yabuki, Ryohiko Ozaki, Seidai Sato, Hiroshi Nokihara, Yasuhiko Nishioka,
A man with non-small-cell lung cancer who was negative for anti-nuclear antibodies was admitted for dyspnea after immune checkpoint inhibitor (ICI) administration. Computed tomography (CT) showed complexed radiologic features, including subpleural and basal predominant reticular shadow with cystic structures and peribronchovascular consolidation. Although we treated him with high-dose steroid under a diagnosis of ICI-related pneumonitis, he developed acute exacerbation of pneumonitis with progressive ...
Tópico(s): Radiopharmaceutical Chemistry and Applications
2022 - Elsevier BV | Respiratory Medicine Case Reports
H. Yamada, Natsuki Matsumoto, Takanori Komaki, Hiroaki Konishi, Yu Kimura, Aoi Son, Hirohiko Imai, Tetsuya Matsuda, Yasuhiro Aoyama, Teruyuki Kondo,
Abstract Three-dimensional (3D) representation of a tumor with respect to its size, shape, location, and boundaries is still a challenge in photoacoustic (PA) imaging using artificial contrast agents as probes. We carried out PA imaging of tumors in mice using 800RS-PMPC, which was obtained by coupling of 800RS, a near-infrared cyanine dye, with PMPC, a highly selective tumor-targeting methacrylate polymer having phosphorylcholine side chains, as a probe. The conjugate 800RS-PMPC forms compact nanoparticles ( ...
Tópico(s): Thermography and Photoacoustic Techniques
2020 - Nature Portfolio | Scientific Reports
Yukiya Kitayama, Takuya Yamada, Kentaro Kiguchi, Aoi Yoshida, Shuhei Hayashi, Hiroaki Akasaka, Kazunori Igarashi, Yuya Nishimura, Yu Matsumoto, Ryohei Sasaki, Eri Takano, Hirobumi Sunayama, Toshifumi Takeuchi,
Gold-nanoparticle-incorporated molecularly imprinted nanogels acquire stealth capabilities in vivo through protein corona regulation using intrinsic dysopsonic proteins. The composite can be used in radiation therapy to treat mouse pancreatic cancer.
Tópico(s): Radiation Therapy and Dosimetry
2022 - Royal Society of Chemistry | Journal of Materials Chemistry B
Yangbin Gao, Xinhua Dai, Yuki Aoi, Yumiko Takebayashi, Liping Yang, Xiaorui Guo, Qiwei Zeng, Hanchuanzhi Yu, Hiroyuki Kasahara, Yunde Zhao,
Indole-3-acetamide (IAM) is the first confirmed auxin biosynthetic intermediate in some plant pathogenic bacteria. Exogenously applied IAM or production of IAM by overexpressing the bacterial iaaM gene in Arabidopsis causes auxin overproduction phenotypes. However, it is still inconclusive whether plants use IAM as a key precursor for auxin biosynthesis. Herein, we reported the isolation IAM HYDROLASE 1 (IAMH1) gene in Arabidopsis from a forward genetic screen for IAM-insensitive mutants that display ...
Tópico(s): Plant-Microbe Interactions and Immunity
2020 - Elsevier BV | Journal of genetics and genomics/Journal of Genetics and Genomics
Yuka Miyake, Syungo Ishikawa, Yu Kimura, Aoi Son, Hirohiko Imai, Tetsuya Matsuda, H. Yamada, Akio Toshimitsu, Teruyuki Kondo,
Recently, we developed novel chiral dendrimer-triamine-coordinated Gd-MRI contrast agents (Gd-MRI CAs), which showed longitudinal relaxivity (r₁) values about four times higher than that of clinically used Gd-DTPA (Magnevist(®), Bayer). In our continuing study of pharmacokinetic differences derived from both the chirality and generation of Gd-MRI CAs, we found that the ability of chiral dendrimer Gd-MRI CAs to circulate within the body can be directly evaluated by in vitro MRI (7 T). In this study, ...
Tópico(s): Dendrimers and Hyperbranched Polymers
2015 - Multidisciplinary Digital Publishing Institute | Sensors
Akihiko Hatano, Hiroyuki Wakana, Nanae Terado, Aoi Kojima, Chisato Nishioka, Yu Iizuka, Takuya Imaizumi, Sanae Uehara,
Unnatural nucleosides are attracting interest as potential diagnostic tools, medicines, and functional molecules.
Tópico(s): Click Chemistry and Applications
2019 - Royal Society of Chemistry | Catalysis Science & Technology
Taku Sasaki, Kae Kato, Aoi Hosaka, Yu Fu, Atsushi Toyoda, Asao Fujiyama, Yoshiaki Tarutani, Tetsuji Kakutani,
Abstract Transposable elements (TEs) are among the most dynamic parts of genomes. Since TEs are potentially deleterious, eukaryotes silence them through epigenetic mechanisms such as repressive histone modifications and DNA methylation. We previously reported that Arabidopsis TEs, called VANDAL s, counteract epigenetic silencing through a group of sequence‐specific anti‐silencing proteins, VANCs. VANC proteins bind to noncoding regions of specific VANDAL copies and induce loss of silent chromatin ...
Tópico(s): Plant Molecular Biology Research
2023 - Springer Nature | EMBO Reports
Satoko Watanabe, Yu Yamamoto, Aoi Kurokawa, Hiroshi Iseki, Taichiro Tanikawa, Masaji Mase,
Virulent fowl adenovirus serotype 4 (FAdV-4) causes hydropericardium syndrome (HPS) with high mortality in chickens, leading to significant economic losses to the poultry industry. The development of an effective vaccine is essential for successful disease control. Here, we produced recombinant fiber-1 protein of FAdV-4, isolated from a Japanese HPS outbreak strain, JP/LVP-1/96, using a baculovirus expression system and evaluated its immunogenicity and protective efficacy. Recombinant fiber-1 protein ...
Tópico(s): Viral gastroenteritis research and epidemiology
2023 - Springer Science+Business Media | Archives of Virology
Yuki Nagata, Akiyoshi Hirayama, Satsuki Ikeda, Aoi Shirahata, Futaba Shoji, Midori Maruyama, Mitsunori Kayano, Masahiko Bundo, Kotaro Hattori, Sumiko Yoshida, Yu-ichi Goto, Katsuya Urakami, Tomoyoshi Soga, Kouichi Ozaki, Shumpei Niida,
Alzheimer's disease (AD) is a most common dementia in elderly people. Since AD symptoms resemble those of other neurodegenerative diseases, including idiopathic normal pressure hydrocephalus (iNPH), it is difficult to distinguish AD from iNPH for a precise and early diagnosis. iNPH is caused by the accumulation of cerebrospinal fluid (CSF) and involves gait disturbance, urinary incontinence, and dementia. iNPH is treatable with shunt operation which removes accumulated CSF from the brain ventricles. ...
Tópico(s): Mitochondrial Function and Pathology
2018 - BioMed Central | Biomarker Research
Ryota Inoue, Takahiro Tsuno, Yu Togashi, Tomoko Okuyama, Aoi Sato, Kuniyuki Nishiyama, Mayu Kyohara, Jinghe Li, Setsuko Fukushima, Tatsuya Kin, Daisuke Miyashita, Yusuke Shiba, Yoshitoshi Atobe, Hiroshi Kiyonari, Kana Bando, A. M. James Shapiro, Kengo Funakoshi, Rohit Kulkarni, Yasuo Terauchi, Jun Shirakawa,
Uncoupling protein 2 (UCP2), a mitochondrial protein, is known to be upregulated in pancreatic islets of patients with type 2 diabetes (T2DM); however, the pathological significance of this increase in UCP2 expression is unclear. In this study, we highlight the molecular link between the increase in UCP2 expression in β-cells and β-cell failure by using genetically engineered mice and human islets. β-cell-specific UCP2-overexpressing transgenic mice (βUCP2Tg) exhibited glucose intolerance and a reduction ...
Tópico(s): Metabolism, Diabetes, and Cancer
2022 - Cell Press | iScience
A. A. Korsheninnikov, K. Yoshida, D.V. Aleksandrov, N. Aoi, Y. Doki, N. Inabe, Makoto Fujimaki, T. Kobayashi, Hiroshi Kumagai, C.-B. Moon, E. Yu. Nikolskii, M.M. Obuti, A. A. Ogloblin, A. Ozawa, S. Shimoura, Takeshi Suzuki, I. Tanihata, Yutaka Watanabe, M. Yanokura,
The reactions 11Li+CD2→10He+X and 11Li+C→10He+X at a beam energy of 61A MeV have been studied by measuring the triple coincidence of 8He and two neutrons. Experimental data show a peak that can be explained as a resonance 10He, which is unbound by 1.2±0.3 MeV and its width is less than 1.2 MeV. Theinvariant mass spectra of 8He+n show a peak that is consistent with the decay 10He→8He+n+n.
Tópico(s): Quantum Chromodynamics and Particle Interactions
1994 - Elsevier BV | Physics Letters B
A.A. Korsheninnikov, K. Yoshida, D.V. Aleksandrov, N. Aoi, Y. Doki, N. Inabe, Makoto Fujimaki, T. Kobayashi, Hiroshi Kumagai, C.-B. Moon, E. Yu. Nikolskii, M.M. Obuti, A. A. Ogloblin, A. Ozawa, S. Shimoura, Toshio Suzuki, I. Tanihata, Yutaka Watanabe, M. Yanokura,
An experimental study of 8He + p elastic scattering and a search for 8He∗ as observed in two independent experimental methods. The obtained parameters of the excited state are E8He∗ = 3.57±0.12 MeV, Γ =0.5±0.35 MeV, Γ4He+4n/Γ6He+n+n ⩽ 5%. Angular distributions for elastic and inelastic scattering are measured. The inelastic angular distribution is consistent with Jπ = 2+ for 8He3.55∗.
Tópico(s): High-pressure geophysics and materials
1993 - Elsevier BV | Physics Letters B
Yin Xu, Mingjun Xiao, Yu Zhu, Jie Wu, Sheng Zhang, Jinrui Zhou,
With the explosive spread of smart mobile devices, Mobile CrowdSensing (MCS) has been becoming a promising paradigm, by which a platform can coordinate a group of workers to complete large-scale data collection tasks using their mobile devices. In this paper, we investigate the incentive mechanism design in MCS systems, taking the freshness of collected data and social benefits into consideration. First, the Age of Information (AoI) metric is introduced to measure the freshness of data. Then, we model ...
Tópico(s): Human Mobility and Location-Based Analysis
2023 - IEEE Computer Society | IEEE Transactions on Mobile Computing