Igor pl�chal, Ilja Trebichavsk�, Yoshihiro Muneta, Yasuyuki Mori,
Cytokine response against Salmonella Typhimurium is traditionally studied in conventional animals.Germ-free animals, however, enable to study response against infection without background effect of other microorganisms.Plasma and ileal inflammatory cytokines in germ-free piglets orally infected with virulent LT2 strain or, with a non-virulent SF1591 rough mutant were quantified by ELISA.In plasma and ileal washes, IFN-γ levels significantly increased in both infected groups.TNF-α and IL-18 were ...
Tópico(s): Animal Nutrition and Physiology
2002 - BioMed Central | Veterinary Research
Naruhiko Muneta, Yasuyuki SHIMIZU,
本論文は, レーザー流速計を用いた基礎実験により, 水制背後の流れを詳細に計測し, 水制により発生する2次流の構造を初めて明らかにした. さらに, この結果を基に水制により発生する2次流のモデル化を行った. このモデルは, 水深平均流速成分に2次元モデルの流速解を用い, これを3次元運動方程式に代入することにより構造物背後に発生する2次流の算出を行うものである.
Tópico(s): Soil, Finite Element Methods
1994 - | Doboku Gakkai Ronbunshu
Yoshihiro Muneta, Yasuyuki Mori, Yoshihiro Shimoji, Yuichi Yokomizo,
We have isolated and sequenced a cDNA that contains the coding sequence of porcine interleukin 18 (IL-18) and the recombinant protein of porcine IL-18 was expressed using the baculovirus system. The open reading frame (ORF) of the porcine IL-18 cDNA is 579 base pairs (bp) in length and encodes 192 amino acids. The predicted amino acid sequence is 76.7%, 64.7% and 61.6% homologous to the predicted human, murine and rat amino acid sequences, respectively. The porcine precursor and mature IL-18 protein ...
Tópico(s): Animal Virus Infections Studies
2000 - Elsevier BV | Cytokine
Osamu Mikami, Midori KUBO, Hideo Murata, Yoshihiro Muneta, Yasuyuki NAKAJIMA, Shigeru Miyazaki, Nobuhiko TANIMURA, Ken Katsuda,
Seven miniature pigs were injected intravenously with deoxynivalenol (DON) at 1 mg/kg body weight; afterward, the number of leukocytes in peripheral blood, the luminol-dependent chemiluminescence of neutrophils, the serum or plasma concentration of cytokines and acute-phase proteins were evaluated to determine the effects of acute exposure to DON on inflammatory responses. White blood cell counts were transiently increased at 3, 6, and 12 hr post-injection (PI) due to the increased number of neutrophils. ...
Tópico(s): Indoor Air Quality and Microbial Exposure
2011 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Alla Šplı́chalová, I Trebichavský, Yoshihiro Muneta, Yasuyuki Mori, Igor Šplı́chal,
Problem: The upregulation of inflammatory substances threatens pregnancy. Interleukin‐18 (IL‐18) is elevated in women who miscarried. The purpose of this study was to develop a pig model of chorioamnionitis to study the effect of bacterial virulence on IL‐18 response in experimentally infected amnion. Method of study: A total of 20,000 colony‐forming units of Escherichia coli (an enteropathogenic O55 strain, EPEC or O86 non‐pathogenic strain) were administered into the amniotic cavity of pig fetuses ...
Tópico(s): Reproductive System and Pregnancy
2005 - Wiley | American Journal of Reproductive Immunology
Yoshihiro Shimoji, Eiji Oishi, Takashi Kitajima, Yoshihiro Muneta, Shinya Shimizu, Yasuyuki Mori,
ABSTRACT We have developed a system in which a foreign antigen is delivered and expressed on the surface of an attenuated strain of Erysipelothrix rhusiopathiae YS-1 and have examined the ability of a such recombinant E. rhusiopathiae strain to function as a mucosal vaccine vector. The C-terminal portion, including two repeat regions, R1 and R2, of the P97 adhesin of Mycoplasma hyopneumoniae strain E-1 was successfully translocated and expressed on the E. rhusiopathiae YS-1 cell surface after it ...
Tópico(s): Bacteriophages and microbial interactions
2002 - American Society for Microbiology | Infection and Immunity
Yoshihiro Shimoji, Eiji Oishi, Yoshihiro Muneta, Hideji Nosaka, Yasuyuki Mori,
The attenuated Erysipelothrix rhusiopathiae YS-19 strain was constructed for the purpose of delivering the C-terminal portion of the Mycoplasma hyopneumoniae P97 adhesin to the mucosal surface of the respiratory tract of pigs. In this study, the efficacy of the YS-19 vaccine against mycoplasmal pneumonia of swine was evaluated. Animal experiments revealed that intranasal immunization of pigs with the YS-19 strain significantly reduced the severity of pneumonic lung lesions caused by M. hyopneumoniae ...
Tópico(s): Pneumonia and Respiratory Infections
2003 - Elsevier BV | Vaccine
Igor Šplı́chal, Ivan Rychlı́k, Daniela Gregorová, Alena Šebková, I Trebichavský, Alla Šplı́chalová, Yoshihiro Muneta, Yasuyuki Mori,
Salmonella protease mutants, clpP and especially htrA, are candidate live oral vaccines in humans. A functional and mature immune system is, however, required to cope with them in mice. Here, we test the cytokine response of highly susceptible germ-free pigs to infection with Salmonella Typhimurium clpP and htrA mutants. Cytokine levels (IL-4, IL-10, IL-18 and IFN-gamma) were measured by ELISA in plasma and washes from the terminal small bowel 24h after oral challenge. Unlike the infection with the ...
Tópico(s): Escherichia coli research studies
2007 - Elsevier BV | Immunobiology
Yoshihiro Muneta, Yoshihiro Shimoji, Yuichi Yokomizo, Yasuyuki Mori,
We have cloned and sequenced a cDNA that contains the coding sequence of porcine interleukin-1 beta (IL-1 beta) converting enzyme (ICE). Using degenerate oligonucleotide primers based on the amino acid sequences of the human, murine, and rat ICE, we performed the reverse transcription polymerase chain reaction (RT-PCR) with total RNA prepared from porcine alveolar macrophages stimulated with lipopolysaccharide (LPS) to clone the cDNA of porcine ICE. The open reading frame (ORF) of the porcine ICE cDNA ...
Tópico(s): interferon and immune responses
1999 - Mary Ann Liebert, Inc. | Journal of Interferon & Cytokine Research
Reiko Nagata, Yoshihiro Muneta, Kazuhiro Yoshihara, Yuichi Yokomizo, Yasuyuki Mori,
Three recombinant proteins, Map10, Map39, and Map41, produced based on nucleotide sequences obtained from the screening of Mycobacterium avium subsp. paratuberculosis genomic library expressed in Escherichia coli significantly elicited gamma interferon production in peripheral blood mononuclear cells from infected cattle. Two of these proteins were members of the PPE protein family.
Tópico(s): Bacteriophages and microbial interactions
2005 - American Society for Microbiology | Infection and Immunity
Yoshihiro Muneta, Osamu Mikami, Yoshihiro Shimoji, Yasuyuki NAKAJIMA, Yuichi Yokomizo, Yasuyuki Mori,
We describe here the development of sandwich enzyme-linked immunosorbent assay (sandwich ELISA) and immunohistochemical staining for porcine interleukin-18 (PoIL-18) and their application to detection of PoIL18 in vivo. Ten anti-PoIL-18 monoclonal antibodies (mAb), all of which were reactive with recombinant PoIL18 by Western blotting, were established. Four (2-C-4, 9-H-6, 11-H-5, and 12-C-12) of 10 neutralized the biologic activity of PoIL-18 to induce interferon- gamma (IFN-gamma) from porcine peripheral ...
Tópico(s): Vector-borne infectious diseases
2000 - Mary Ann Liebert, Inc. | Journal of Interferon & Cytokine Research
Yoshihiro Muneta, Yoshihiro Shimoji, Shigeki Inumaru, Yasuyuki Mori,
We isolated and sequenced cDNA that contained the coding sequence of porcine Fas ligand (FasL). Using mixed oligonucleotide primers based on the 5' and 3' nucleotide sequences conserved among human, murine, and rat FasL, we performed the reverse transcription polymerase chain reaction (RT-PCR) with total RNA prepared from porcine thymocytes stimulated with 5 μg/ml concanavalin A (ConA) to clone the cDNA of porcine FasL. The open reading frame (ORF) of porcine FasL cDNA was 849 base pairs (bp) in length ...
Tópico(s): RNA Interference and Gene Delivery
2001 - Mary Ann Liebert, Inc. | Journal of Interferon & Cytokine Research
Yoshihiro Muneta, Hong Kun Zhao, Shigeki Inumaru, Yasuyuki Mori,
In this report, a hybrid baculovirus expression system, which means a hybrid virus of the Autographa californica nuclear polyhedrosis virus and the Bombyx mori nuclear polyhedrosis virus, was used for the large-scale production of porcine mature interleukin-18 (IL-18) in silkworms. Two recombinant hybrid baculoviruses containing cDNA of the porcine precursor IL-18 and the porcine caspase-1 were constructed and were used to infect silkworm larvae. After the co-infection of the two viruses, porcine ...
Tópico(s): Virus-based gene therapy research
2003 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Naruhiko Muneta, Yasuyuki SHIMIZU, Tadaoki ITAKURA,
It is one of the important subject in river planning works to predict flow field and bed topography in the region around spur-dikes. Especially, the qualitative understanding of the mechanism of sediment deposition produced by spur-dikes enables the effective design of spur-dike collocation. However, since various complex phenomena are coexisting in the neighborhood of spur-dikes, it is necessary to investigate which phenomenon is essential for the sediment deposition around spur-dikes. In this ...
Tópico(s): Hydrology and Watershed Management Studies
1996 - | PROCEEDINGS OF HYDRAULIC ENGINEERING
Yoshihiro Muneta, Shigeki Inumaru, Yoshihiro Shimoji, Yasuyuki Mori,
We previously reported that the precursor form of porcine interleukin-18 (IL-18) expressed by the baculovirus system was able to be secreted efficiently into the supernatant of insect cells, whereas only small amounts of mature IL-18 were secreted from insect cells. As insect cells do not normally have the IL-1β converting enzyme (caspase-1), which is required for processing of the precursor IL-18 into the mature IL-18, we recently cloned porcine caspase-1 cDNA. In this study, we constructed a recombinant ...
Tópico(s): Transgenic Plants and Applications
2001 - Mary Ann Liebert, Inc. | Journal of Interferon & Cytokine Research
Yoshihiro Muneta, Yoshihiro Shimoji, Yuichi Yokomizo, Yasuyuki Mori,
We have recently reported the cloning and expression of porcine interleukin-18 (IL-18). In this study, we describe the production of anti-porcine IL-18 monoclonal antibodies (mAb) and their use in the purification of a large amount of recombinant porcine IL-18 by immunoaffinity column chromatography. Five monoclonal antibodies (2-2-B, 2-5-B, 2-13-C, 3-1-C and 5-3-B) were established and characterized. Three (2-2-B, 3-1-C and 5-3-B) of them were of IgG1 subclass, and the other two were IgMs. Epitope ...
Tópico(s): Herpesvirus Infections and Treatments
2000 - Elsevier BV | Journal of Immunological Methods
Yoshihiro Muneta, Yu Minagawa, Yoshihiro Shimoji, Yohsuke Ogawa, Hirokazu Hikono, Yasuyuki Mori,
In this study, several cytokine responses were investigated during Mycoplasma hyopneumoniae (Mhp) infection using a gnotobiotic infection model. We found that several inflammatory cytokines (IL-1beta, IL-8, IL-18, and TNF-alpha) and an anti-inflammatory cytokine IL-10 were induced from peripheral blood mononuclear cells (PBMC) of germ-free (GF) piglets stimulated with heat killed Mhp whole antigens, but no IFN-gamma and IL-4 were induced by Mhp. After the intranasal infection of Mhp, IL-1beta, IL-8, ...
Tópico(s): Aquaculture disease management and microbiota
2008 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Yoshihiro Muneta, Hidekazu Nagaya, Yu Minagawa, Chiaki Enomoto, Sayaka Matsumoto, Yasuyuki Mori,
Tópico(s): Virus-based gene therapy research
2004 - Springer Science+Business Media | Biotechnology Letters
Yoshihiro Muneta, Noriko Goji, Noriko M. Tsuji, Osamu Mikami, Yoshihiro Shimoji, Yasuyuki NAKAJIMA, Yuichi Yokomizo, Yasuyuki Mori,
In this study, we investigated the expression of interleukin-18 (IL-18) in porcine airway and intestinal epithelium. We found constitutive protein expression of precursor IL-18 in primary culture of porcine airway epithelium. Immunohistochemical staining revealed that porcine IL-18 was localized in the porcine airway epithelium and that it was significantly upregulated with experimental endotoxemia induced by Escherichia coli lipopolysaccharide (LPS) inoculation. We also confirmed by immunohistochemical ...
Tópico(s): Immune Cell Function and Interaction
2002 - Mary Ann Liebert, Inc. | Journal of Interferon & Cytokine Research
Kazuhiro Yoshihara, Reiko Nagata, Yoshihiro Muneta, Shigeki Inumaru, Yuichi Yokomizo, Yasuyuki Mori,
The generation of multinucleated giant cells (MGC) from cells of the bovine monocyte-macrophage lineage was investigated. Freshly isolated monocytes were incubated with the conditioned medium (CM) of peripheral blood mononuclear cell cultures treated with Concanavalin A for 1-4 days (CM1 to CM4). Only CM1 generated MGC despite similar concentrations of IFNγ in all CMs. Nevertheless, MGC formation from monocytes was enhanced by adding either macrophage colony-stimulating factor (M-CSF) or granulocyte- ...
Tópico(s): Immunotherapy and Immune Responses
2004 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Yoshihiro Muneta, Yu Minagawa, Yoshihiro Shimoji, Reiko Nagata, Philip F. Markham, Glenn F. Browning, Yasuyuki Mori,
Little is known about the detail of the immune response during infection of pigs with Mycoplasma hyopneumoniae (Mhp). To further understand this important porcine pathogen, we examined the interleukin-18 (IL- 18) response in experimentally infected piglets. We found that large amounts of IL-18 were produced in the bronchoalveolar lavage fluids (BALF) of pigs experimentally infected with Mhp. However, the concentration of interferon-γ (IFN-γ) in the same BALF was negatively correlated with that of IL- ...
Tópico(s): Herpesvirus Infections and Treatments
2006 - Mary Ann Liebert, Inc. | Journal of Interferon & Cytokine Research
Yoshihiro Muneta, Kazuhiro Yoshihara, Yu Minagawa, Reiko Nagata, Yasuyuki Mori, Takahiro Yamaguchi, Kazuaki Takehara,
Abstract In this study, we examined the concentration of bovine IL‐18 in the sera of pregnant cows, their fetuses and newborn calves, and in colostrum in order to examine the role of IL‐18 in bovine pregnancy and the neonatal period. A sandwich‐ELISA to quantify bovine IL‐18 was established using anti‐porcine IL‐18 monoclonal antibodies, which cross‐reacted with bovine IL‐18, and used it to measure the concentration of bovine IL‐18 in the sera of pregnant cows, their fetuses and newborn calves, ...
Tópico(s): Urticaria and Related Conditions
2005 - Taylor & Francis | Journal of Immunoassay and Immunochemistry
Naruhiko Muneta, Yasuyuki SHIMIZU,
In numerical analyses (two-dimensional models) of flows and river-bed fluctions (variation) around spur-dikes, reproducibility is poor when the transverse shear is not accurate. To investigate the mechanism of flows induced by spur-dikes, measurement was made with laser current meters measuring two components simultaneously, and this allowed an adequate determination of the conditions of the flow around spur-dikes. The application of Plandtl's transport theory in estimating the Reynolds stress was ...
Tópico(s): Hydrology and Watershed Management Studies
1993 - | PROCEEDINGS OF HYDRAULIC ENGINEERING
Yoshihiro Muneta, Reiko Kikuma, Kazuhiro Yoshihara, Yasuyuki Mori,
Bovine interleukin-21 (IL-21) cDNA was cloned and sequenced from bovine peripheral blood lymphocytes (PBLs) stimulated with 10 microg/ml concanavalin A (ConA), 10 microg/ml phytohemagglutinin (PHA), and 50 ng/ml phorbol 12-myristate 13-acetate (PMA) for 48 h. The open reading frame of the bovine IL-21 cDNA is 459 bp in length and encodes 152 amino acids. The predicted amino acid sequence is 78.2 and 58.5% homologous to the human and murine IL-21 amino acid sequences, respectively. Recombinant bovine ...
Tópico(s): Immune Cell Function and Interaction
2003 - Elsevier BV | Veterinary Immunology and Immunopathology
Yoshihiro Muneta, Hirohide Uenishi, Reiko Kikuma, Kazuhiro Yoshihara, Yoshihiro Shimoji, Ryûji Yamamoto, Noriyuki Hamashima, Yuichi Yokomizo, Yasuyuki Mori,
We successfully cloned and sequenced porcine toll-like receptor (TLR2) and TLR6 cDNA from porcine alveolar macrophages stimulated with 10 μg/ml lipopolysaccharide (LPS). The open reading frames (ORFs) of the porcine TLR2 and TLR6 cDNA were shown to be 2358 and 2391 bp in length and to encode 785 and 796 amino acids, respectively. The predicted amino acid sequence of porcine TLR2 was 72.3% homologous to human TLR2 and 61.0% homologous to murine TLR2. That of porcine TLR6 was 74.4% homologous to human ...
Tópico(s): Pneumonia and Respiratory Infections
2003 - Mary Ann Liebert, Inc. | Journal of Interferon & Cytokine Research
Yoshihiro Muneta, Yoshihiro Shimoji, Yasuyuki Mori,
We cloned and sequenced cDNA that contained the coding sequence of porcine caspase-3. The open reading frame (ORF) of porcine caspase-3 cDNA was 834 base pairs (bp) in length and encoded 277 amino acids. The predicted amino acid sequence was 88.4%, 86.6%, and 87.7% homologous to the predicted human, murine, and rat amino acid sequences, respectively. The activity of caspase-3 in porcine renal tubular cell line PK15 after recombinant porcine Fas ligand (FasL) stimulation was examined. The enzymatic ...
Tópico(s): RNA Interference and Gene Delivery
2001 - Mary Ann Liebert, Inc. | Journal of Interferon & Cytokine Research
Yoshihiro Muneta, Hirohide Uenishi, Ryûji Yamamoto, Kazuhiro Yoshihara, Hiroshi Yasue, Takashi Awata, Yasuyuki Mori,
We cloned and sequenced a cDNA that contains the coding sequence of the porcine interleukin-18 receptor alpha chain (PoIL-18Ralpha). Based on the conserved nucleotide sequences between human (HuIL-18Ralpha) and murine IL-18Ralpha (MuIL-18Ralpha), we performed reverse transcription-polymerase chain reaction (RT-PCR) with total RNA prepared from porcine peripheral blood lymphocytes (PBLs) stimulated with PoIL-12 to clone the cDNA of PoIL-18Ralpha. The open reading frame (ORF) of the PoIL-18Ralpha cDNA is ...
Tópico(s): T-cell and B-cell Immunology
2002 - Mary Ann Liebert, Inc. | Journal of Interferon & Cytokine Research
Hidekazu Nagaya, Yoshihiro Muneta, Chiaki Enomoto, Sayaka Matsumoto, Yuichi Yokomizo, Yasuyuki Mori,
Tópico(s): Protein purification and stability
2004 - Springer Science+Business Media | Biotechnology Letters
Yoshihiro Muneta, Reiko Kikuma, Hirohide Uenishi, Tomoaki Hoshino, Kazuhiro Yoshihara, Maiko Tanaka, Noriyuki Hamashima, Yasuyuki Mori,
A pig interleukin-21 (IL-21) cDNA was successfully cloned and sequenced from porcine peripheral blood lymphocytes (PBL) stimulated with 10 μg/ml concanavalin A (ConA), 10 μg/ml phytohemagglutinin P (PHA), 50 ng/ml phorbol 12-myristate 13-acetate (PMA), and 0.5 μg/ml anti-porcine CD3 antibody for 48 hr. The open reading frame of the porcine IL-21 cDNA is 459 base pairs in length and encodes 152 amino acids. The predicted amino acid sequence of the porcine IL-21 shows 86.2%, 77.7%, and 58.4% identity to ...
Tópico(s): Virus-based gene therapy research
2004 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Naruhiko Muneta, Yasuharu WATANABE, Yasuyuki SHIMIZU,
In river planning, it is necessary to study the influence of river construction to the river flow and the river bed. A flow affected by the river construction is a three-dimensional complicated flow.Sand deposition and scour around the construction is caused by this complicated flow which make it difficult to grasp the river change with the construction.To investigate the mechanism of flows induced by spur-dike, we need the field survey around a spur-dike.we are reporting new results obtained from ...
Tópico(s): Energy, Environment, Agriculture Analysis
1995 - | PROCEEDINGS OF HYDRAULIC ENGINEERING