Kohei Tahara, Hiromasa Tomida, Yousuke Ito, Suguru Tachikawa, Risako Onodera, Hiroyuki Tanaka, Yuichi Tozuka, Hirofumi Takeuchi,
Drug inhalation provides localized drug therapy for respiratory diseases. However, the therapeutic efficacy of inhaled drugs is limited by rapid clearance from the lungs. Small hydrophilic compounds have short half-lives to systemic absorption. We developed a liposomal formulation as a sustained-release strategy for pulmonary delivery of procaterol hydrochloride (PRO), a short-acting pulmonary β2-agonist for asthma treatment. After PRO-loaded liposomes were prepared using a pH gradient (remote loading) ...
Tópico(s): Asthma and respiratory diseases
2016 - Elsevier BV | International Journal of Pharmaceutics
Miyuki Tanaka, Eriko Misawa, Yousuke Ito, Noriko Habara, Kouji Nomaguchi, Muneo Yamada, Tomohiro Toida, Hirotoshi Hayasawa, Mitunori Takase, Masanori Inagaki, Ryuuichi Higuchi,
The genus Aloe in the family Liliaceae is a group of plants including Aloe vera (Aloe barbadensis MILLER) and Aloe arborescens (Aloe arborescens MILLER var. natalensis BERGER) that are empirically known to have various medical efficacies. In the present study, we evaluated the anti-hyperglycemic effect of Aloe vera gel and isolated a number of compounds from the gel. On the basis of spectroscopic data, these compounds were identified as lophenol, 24-methyl-lophenol, 24-ethyl-lophenol, cycloartanol, ...
Tópico(s): Ginseng Biological Effects and Applications
2006 - Pharmaceutical Society of Japan | Biological and Pharmaceutical Bulletin
Keitaro Mashimo, Ayumi Usui‐Ouchi, Yousuke Ito, Rei Wakasa-Arai, Norihiko Yokoi, Satoshi Kawasaki, Akira Murakami, Akira Matsuda, Nobuyuki Ebihara,
Vernal keratoconjunctivitis (VKC) is a severe and recurrent allergic conjunctivitis, the mechanism of which is not well understood. In this study, we investigated the role of oncostatin M (OSM) in the pathogenesis of VKC, with a focus on tissue remodeling. Clinical and experimental. The OSM concentrations in tear fluid samples obtained from VKC patients and healthy controls were measured using ELISA, and the expression of OSM mRNA and protein in giant papillae resected from VKC patients was investigated ...
Tópico(s): Immune Response and Inflammation
2021 - Springer Science+Business Media | Japanese Journal of Ophthalmology
Naozumi Ohnishi, Hiromasa Tomida, Yousuke Ito, Kohei Tahara, Hirofumi Takeuchi,
A novel in vitro release test methodology for a liposome formulation was developed using a column-switching high-performance liquid chromatography (HPLC) system. Doxorubicin (DXR) liposome formulations were used as a model. A DXR liposome formulation was dispersed into a release medium, and the dispersion fluid was directly injected at predetermined time points into the column-switching HPLC system. To evaluate the release profile, this system can be used for determining the released and encapsulated ...
Tópico(s): Surfactants and Colloidal Systems
2014 - Pharmaceutical Society of Japan | Chemical and Pharmaceutical Bulletin
Sho Nambara, Takaaki Masuda, Miki Nishio, Shotaro Kuramitsu, Taro Tobo, Yushi Ogawa, Qingjiang Hu, Tomohiro Iguchi, Yousuke Kuroda, Shuhei Ito, Hidetoshi Eguchi, K Sugimachi, Hiroshi Saeki, Eiji Oki, Yoshihiko Maehara, Akira Suzuki, Koshi Mimori,
... Ogawa 5 , Qingjiang Hu 1 , Tomohiro Iguchi 6 , Yousuke Kuroda 1 , Shuhei Ito 1 , Hidetoshi Eguchi 1 , Keishi Sugimachi 1, 6 , ...
Tópico(s): Cancer Mechanisms and Therapy
2017 - Impact Journals LLC | Oncotarget
Yousuke Shimoda, Hisashi Itô, Ayumi Tanaka,
Summary Chlorophyll is a deleterious molecule that generates reactive oxygen species and must be converted to non‐toxic molecules during plant senescence. The degradation pathway of chlorophyll a has been determined; however, that of chlorophyll b is poorly understood, and multiple pathways of chlorophyll b degradation have been proposed. In this study, we found that chlorophyll b is degraded by a single pathway, and elucidated the importance of this pathway in avoiding cell death. In order to determine ...
Tópico(s): Plant nutrient uptake and metabolism
2012 - Wiley | The Plant Journal
M. Tokumaru, M. Kojima, K. Fujiki, K. Maruyama, Yousuke Maruyama, Hirotaka Ito, Tomoya Iju,
A large‐aperture radiotelescope called the Solar Wind Imaging Facility (SWIFT) has been developed at the Toyokawa Observatory of the Solar‐Terrestrial Environment Laboratory (STEL), Nagoya University. The SWIFT is dedicated to interplanetary scintillation (IPS) observations of the solar wind at 327 MHz, the same frequency as that of the existing STEL IPS system. The aim of this instrument is to improve the spatial and temporal resolutions of tomographic reconstructions from STEL IPS observations by ...
Tópico(s): Planetary Science and Exploration
2011 - Wiley | Radio Science
Mariko Torimitsu, Ryouhei NAGASE, Megumi Yanagi, Miyuki Homma, Yousuke Sasai, Yukihiko Ito, Kousuke HAYAMIZU, Shouta NONAKA, Takashi Hosono, Mitsuo Kise, Taiichiro Seki, Toyohiko Ariga,
Pre-germinated brown rice (PR) has been developed industrially in order to enhance the nutritional functions of its source material, brown rice (BR). The present study was aimed at clarifying the effect of PR on the type 2 diabetes mellitus. We employed Otsuka Long-Evans Tokushima Fatty (OLETF) rats as a model of type-2 diabetes mellitus. OLETF rats were fed on either PR or white rice (WR) from the age of 4 to 35 wk. Age-matched male Long-Evans Tokushima Otsuka (LETO) rats as a non-diabetic control were ...
Tópico(s): Food composition and properties
2010 - Center for Academic Publications Japan | Journal of Nutritional Science and Vitaminology
Naveen Chandra, Prabir K. Patra, Yousuke Niwa, Akihiko Ito, Yosuke Iida, Daisuke Goto, Shinji Morimoto, Masayuki Kondo, Masayuki Takigawa, Tomohiro Hajima, Michio Watanabe,
Abstract. Global and regional sources and sinks of carbon across the earth's surface have been studied extensively using atmospheric carbon dioxide (CO2) observations and atmospheric chemistry-transport model (ACTM) simulations (top-down/inversion method). However, the uncertainties in the regional flux distributions remain unconstrained due to the lack of high-quality measurements, uncertainties in model simulations, and representation of data and flux errors in the inversion systems. Here, we assess ...
Tópico(s): Atmospheric chemistry and aerosols
2022 - Copernicus Publications | Atmospheric chemistry and physics
Yuka Miyamae, Toshiya Hayashi, Hitoshi Yonezawa, Jin Fujihara, Yousuke Matsumoto, Takafumi Ito, Takaya Tsubota, Koji Ishii,
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is the cause of novel coronavirus disease 2019 (COVID-19), was first reported in Wuhan, China, and now has spread across the world as a global pandemic. The propagation from asymptomatic polymerase chain reaction (PCR)-positive individuals represents a complicating factor in the efforts to control the COVID-19 pandemic. We examined the course of PCR assays and the duration of viral shedding in 23 asymptomatic or mild COVID-19 patients ...
Tópico(s): COVID-19 Clinical Research Studies
2020 - Elsevier BV | International Journal of Infectious Diseases
Sho Nambara, Takaaki Masuda, Yuta Kobayashi, Kuniaki Sato, Taro Tobo, Kensuke Koike, Miwa Noda, Yushi Ogawa, Yousuke Kuroda, Shuhei Ito, Hidetoshi Eguchi, K Sugimachi, Koshi Mimori,
Abstract Chromosome 7q (Ch.7q) is clonally amplified in colorectal cancer (CRC). We aimed to identify oncogenes on Ch.7q that are overexpressed through DNA copy number amplification and determine the biological and clinical significance of these oncogenes in CRC. We identified general transcription factor 2I repeat domain‐containing protein 1 ( GTF2IRD1 ) as a potential oncogene using a CRC dataset from The Cancer Genome Atlas with a bioinformatics approach. We measured the expression of GTF2IRD1 in 98 ...
Tópico(s): RNA Research and Splicing
2019 - Wiley | Cancer Science
Yousuke Shimoda, Hisashi Itô, Ayumi Tanaka,
Abstract Pheophytin a is an essential component of oxygenic photosynthetic organisms because the primary charge separation between chlorophyll a and pheophytin a is the first step in the conversion of light energy. In addition, conversion of chlorophyll a to pheophytin a is the first step of chlorophyll degradation. Pheophytin is synthesized by extracting magnesium (Mg) from chlorophyll; the enzyme Mg-dechelatase catalyzes this reaction. In this study, we report that Mendel's green cotyledon gene, ...
Tópico(s): Plant Stress Responses and Tolerance
2016 - Oxford University Press | The Plant Cell
Naoki Sasahira, Hiroshi Kawakami, Hiroyuki Isayama, Rie Uchino, Yousuke Nakai, Yukiko Ito, Saburo Matsubara, Hirotoshi Ishiwatari, Minoru Uebayashi, Hiroshi Yagioka, Osamu Togawa, Nobuo Toda, Naoya Sakamoto, Junji Kato, Kazuhiko Koike,
Background and study aims: There are no guidelines for the timing of conversion from a single-guidewire to a double-guidewire technique to facilitate selective bile duct cannulation and reduce post-endoscopic retrograde cholangiopancreatography pancreatitis (PEP), when using wire-guided cannulation. We investigated whether early conversion to the double-guidewire method, at first unintentional insertion of a guidewire into the pancreatic duct, facilitated selective bile duct cannulation and reduced PEP compared ...
Tópico(s): Pancreatic and Hepatic Oncology Research
2015 - Thieme Medical Publishers (Germany) | Endoscopy
Fuko Matsuda, Keisuke NAKATSUKASA, Yuta Suetomi, Yousuke Naniwa, Daisuke Ito, Naoko Inoue, Yoshihiro Wakabayashi, Hiroaki Okamura, Kei‐ichiro Maeda, Yoshihisa Uenoyama, Hiroko Tsukamura, Satoshi Ohkura,
A luteinising hormone (LH) surge is fundamental to the induction of ovulation in mammalian females. The administration of a preovulatory level of oestrogen evokes an LH surge in ovariectomised females, whereas the response to oestrogen in castrated males differs among species; namely, the LH surge‐generating system is sexually differentiated in some species (e.g. rodents and sheep) but not in others (e.g. primates). In the present study, we aimed to determine whether there is a functional LH surge‐ ...
Tópico(s): Plant Reproductive Biology
2014 - Wiley | Journal of Neuroendocrinology
Takashi Sasaki, Hiroyuki Isayama, Yousuke Nakai, Yukiko Ito, Ichiro Yasuda, Nobuo Toda, Hirofumi Kogure, Keiji Hanada, Hiroyuki Maguchi, Naoki Sasahira, Hideki Kamada, Tsuyoshi Mukai, Yoshihiro Okabe, Osamu Hasebe, Iruru Maetani, Kazuhiko Koike,
Tópico(s): Oral and gingival health research
2013 - Springer Science+Business Media | Cancer Chemotherapy and Pharmacology
Yousuke Ooyama, Genta Ito, Hiroshi Fukuoka, Tomoya Nagano, Yusuke Kagawa, Ichiro Imae, Kenji Komaguchi, Yutaka Harima,
This paper reports that mechanofluorochromism is found for a series of benzofuro[2,3-c]oxazolo[4,5-a]carbazole-type fluorophores (1a–5a) with different p-substituted phenyl groups as acceptor and dibutylamino groups as donor. Grinding of as-recrystallized dyes 1a–3a with strong electron-accepting group induces a fluorescent color change with an enhanced quantum yield and the fluorescent color is recovered by heating or exposure to solvent vapor. On the basis of experimental results and semi-empirical ...
Tópico(s): Porphyrin and Phthalocyanine Chemistry
2010 - Elsevier BV | Tetrahedron
Yousuke Ooyama, Genta Ito, Kohei Kushimoto, Kenji Komaguchi, Ichiro Imae, Yutaka Harima,
Heteropolycyclic donor–π-acceptor (D–π-A) structural isomers of benzofuro[2,3-c]oxazolo[4,5-a]carbazole-type (2a–f) and benzofuro[2,3-c]oxazolo[5,4-a]carbazole-type fluorescent dyes (3a–f), which differ in the position of oxygen and nitrogen atoms of the oxazole ring, have been synthesized, and their photophysical and electrochemical properties have been investigated. The fluorescent dyes 2a–f exhibit much stronger intramolecular charge transfer (ICT) absorption and fluorescence bands than 3a–f. Both ...
Tópico(s): Photochromic and Fluorescence Chemistry
2010 - Royal Society of Chemistry | Organic & Biomolecular Chemistry
Hiroyuki Isayama, Takao Kawabe, Yousuke Nakai, Yukiko Ito, Osamu Togawa, Hirofumi Kogure, Yoko Yashima, Hiroshi Yagioka, Saburo Matsubara, Takashi Sasaki, Naoki Sasahira, Kenji Hirano, Takeshi Tsujino, Minoru Tada, Masao Omata,
Tópico(s): Cholangiocarcinoma and Gallbladder Cancer Studies
2009 - Springer Science+Business Media | Surgical Endoscopy
Takashi Sasaki, Hiroyuki Isayama, Yousuke Nakai, Yukiko Ito, Hirofumi Kogure, Osamu Togawa, Nobuo Toda, Ichiro Yasuda, Osamu Hasebe, Iruru Maetani, Naoki Sasahira, Kenji Hirano, Takeshi Tsujino, Minoru Tada, Masao Omata,
Tópico(s): Pancreatic and Hepatic Oncology Research
2009 - Springer Science+Business Media | Cancer Chemotherapy and Pharmacology
Satoaki Matoba, Tetsuya Tatsumi, Toyoaki Murohara, Tsutomu Imaizumi, Yousuke Katsuda, Masaaki Ito, Yoshihiko Saito, Shiro Uemura, Hiroshi Suzuki, Shinya Fukumoto, Yasutaka Yamamoto, Rie Onodera, Satoshi Teramukai, Masanori Fukushima, Hiroaki Matsubara,
Angiogenic cell therapy by intramuscular injection of autologous bone marrow mononuclear cells was first attempted in patients with peripheral artery disease (PAD) with critical limb ischemia, and the feasibility was shown by a randomized controlled Therapeutic Angiogenesis by Cell Transplantation (TACT) study. The present study was designed to assess the 3-year safety and clinical outcomes of this angiogenic cell therapy by investigating the mortality and leg amputation-free interval as primary end ...
Tópico(s): Mesenchymal stem cell research
2008 - Elsevier BV | American Heart Journal
Jiro Nakamoto, Ryusuke Torisu, Rika Aoki, Yoshitaka Kimura, Mitsugi Yasuda, K. Shiota, Yousuke Yamamoto, Susumu Ito,
Gastric carcinomas have been classified into the differentiated and undifferentiated type, on the basis of its tendency to gland formation. As a result of recent advances in mucin histochemistry, mucin phenotypes of gastric carcinomas have been investigated. However, no consensus on the evaluation of the grade of malignancy of early gastric carcinomas regarding mucin phenotype expression has developed. To address this issue, we evaluated the lymph node metastasis rate and proliferative activity ...
Tópico(s): Esophageal Cancer Research and Treatment
2007 - University of Tokushima | The Journal of Medical Investigation
Junjiro Kubo, Takeharu Chishaki, Natsumi Nakamura, Tadashi Muramatsu, Yousuke Yamamoto, Masamitsu Ito, Hitoshi Saitou, Takeshi Kukidome,
The purpose of this study was to investigate differences in fat-free mass and thicknesses of various muscles among judo athletes of different performance levels. The subjects were 69 male judo athletes of 3 different performance levels. Group A was composed of athletes who participated in the Olympic Games or Asian Games (n = 13). Groups B (n = 21) and C (n = 35) were composed of judo athletes at a university who did or did not participate in intercollegiate competitions (including qualifying matches), respectively. ...
Tópico(s): Sports Performance and Training
2006 - Lippincott Williams & Wilkins | The Journal of Strength and Conditioning Research
Junjiro Kubo, Takeharu Chishaki, Natsumi Nakamura, Tadashi Muramatsu, Yousuke Yamamoto, Masamitsu Ito, Hitoshi Saitou, Takeshi Kukidome,
The purpose of this study was to investigate differences in fat-free mass and thicknesses of various muscles among judo athletes of different performance levels. The subjects were 69 male judo athletes of 3 different performance levels. Group A was composed of athletes who participated in the Olympic Games or Asian Games (n = 13). Groups B(n = 21) and C (n = 35) were composed of judo athletes at a university who did or did not participate in intercollegiate competitions (including qualifying matches), respectively. ...
Tópico(s): Body Composition Measurement Techniques
2006 - Lippincott Williams & Wilkins | The Journal of Strength and Conditioning Research
Hirohisa Matsuda, Yousuke Fujimoto, Syoji Ito, Yutaka Nagasawa, Hiroshi Miyasaka, Tsuyoshi Asahi, Hiroshi Masuhara,
Femtosecond near-infrared laser microscope was developed with a home-built cavity-dumped chromium:forsterite laser as a light source centered at 1.26 microm. Optimization of the pulse duration achieved 35 fs fwhm at the sample position of the microscope after passing through a 100x objective. This system was applied to the detection of multiphoton fluorescence of some organic microcrystals. Excitation intensity dependence and the interferometric autocorrelation detection of the fluorescence clearly ...
Tópico(s): Spectroscopy Techniques in Biomedical and Chemical Research
2005 - American Chemical Society | The Journal of Physical Chemistry B
Ayako Oyane, Masaki Uchida, Yousuke Ishihara, Atsuo Ito,
Mechanism of formation of a laminin-apatite composite layer on the surface of an ethylene-vinyl alcohol copolymer (EVOH) using a liquid phase coating process was investigated by transmission electron microscopy (TEM). In this coating process, an EVOH substrate is alternately dipped in calcium and phosphate solutions, and then immersed in a laminin-containing calcium phosphate (LCP) solution. From the results obtained by the present study, formation of the laminin-apatite composite layer on EVOH is ...
Tópico(s): Polymer Surface Interaction Studies
2005 - Trans Tech Publications | Key engineering materials
Toshio Mikami, Kayoko Kita, Seiji Tomita, GANG-JIAN QU, YOUSUKE TASAKI, Akira Ito,
To assess whether allantoin levels in serum and urine are influenced by exhaustive and moderate exercise and whether allantoin is a useful indicator of exercise-induced oxidative stress in humans, we made subjects perform exhaustive and moderate (100% and 40% VO2max) cycling exercise and examined the levels of allantoin, thiobarbituric acid reactive substances (TBARS) and urate in serum and urine. Immediately after exercise at 100% VO2max, the serum allantoin/urate ratio was significantly elevated ...
Tópico(s): Muscle metabolism and nutrition
2000 - Taylor & Francis | Free Radical Research
Yousuke Shimizu, Y. Ito, Yosiharu IIDA,
Tópico(s): Microstructure and mechanical properties
1986 - Springer Science+Business Media | Metallurgical Transactions A
Hideo Inagaki, Kengo Hayashi, Yousuke Takaoka, Hibiki Ito, Yuki Fukumoto, Ayaka Yajima-Nakagawa, Xi Chen, Miyuki Shimosato-Nonaka, Emmi Hassett, Kodai Hatakeyama, Yuko Hirakuri, Masanobu Ishitsuka, Emi Yumoto, Tomoko Sakazawa, Masashi Asahina, Ken‐ichi Uchida, Kazunori Okada, Hisakazu Yamane, Minoru Ueda, Koji Miyamoto,
Abstract Jasmonic acid (JA) regulates plant growth, development and stress responses. Coronatine insensitive 1 (COI1) and jasmonate zinc-finger inflorescence meristem-domain (JAZ) proteins form a receptor complex for jasmonoyl-l-isoleucine, a biologically active form of JA. Three COIs (OsCOI1a, OsCOI1b and OsCOI2) are encoded in the rice genome. In the present study, we generated mutants for each rice COI gene using genome editing to reveal the physiological functions of the three rice COIs. The oscoi2 ...
Tópico(s): Plant Parasitism and Resistance
2022 - Oxford University Press | Plant and Cell Physiology
Shin Ishihara, Hidetaka Yamamoto, Takeshi Iwasaki, Yu Toda, Takeo Yamamoto, Masato Yoshimoto, Yoshihiro Ito, Yousuke Susuki, Kengo Kawaguchi, Izumi Kinoshita, Yuichi Yamada, Kenichi Kohashi, Toshifumi Fujiwara, Nokitaka Setsu, Makoto Endo, Yoshihiro Matsumoto, Yuko Kakuda, Yasuharu Nakashima, Yoshinao Oda,
In rare cases, giant cell tumor of bone (GCTB) can undergo primary or secondary malignant transformation to malignant giant cell tumor of bone (MGCTB), but the details of the molecular alterations are still unclear. The present study aimed to elucidate the clinicopathologic and molecular features of MGCTBs based on immunohistochemistry, fluorescence in situ hybridization (FISH) and next generation sequencing (NGS) of nine MGCTBs (five primary and four secondary). Seven (78%) of 9 MGCTBs were immunohistochemically ...
Tópico(s): Sarcoma Diagnosis and Treatment
2021 - Elsevier BV | Modern Pathology
Ken Horibata, Akiko Tanoue, Masaaki Ito, Yousuke Takemura,
We investigated the relationship between renal function and serum magnesium concentration in elderly patients treated with magnesium oxide (MgO) in an outpatient setting of an urban hospital in Japan.In the present study, 44 elderly outpatients (23 patients with constipation treated with daily oral MgO and 21 untreated patients in the control group) who visited Kameyama municipal medical center were enrolled. Variables were age, sex, weight, height, serum magnesium concentration, serum blood urea ...
Tópico(s): Therapeutic Uses of Natural Elements
2015 - Wiley | Geriatrics and gerontology international/Geriatrics & gerontology international