Jackson J. Liang, Benjamín A. Shaw, J. M. Bennett, Neal Krause, E Kobayashi, Taro Fukaya, Yuki Sugihara,
Objectives. We sought to depict how trajectories of functional status are related to the average changes in self-rated health and its underlying trajectories.
Tópico(s): Aging and Gerontology Research
2007 - Oxford University Press | The Journals of Gerontology Series B
Yu Wakabayashi, Taiga Tamiya, Ichiro Takada, Tomohiro Fukaya, Yuki Sugiyama, Naoko Inoue, Akihiro Kimura, Rimpei Morita, Ikko Kashiwagi, Tomohito Takimoto, Masatoshi Nomura, Akihiko Yoshimura,
Suppression of IL-2 βproduction from T cells is an important process for the immune regulation by TGF-β. However, the mechanism by which this suppression occurs remains to be established. Here, we demonstrate that Smad2 and Smad3, two major TGF-β-downstream transcription factors, are redundantly essential for TGF-β-mediated suppression of IL-2 production in CD4+ T cells using Smad2- and Smad3-deficient T cells. Both Smad2 and Smad3 were recruited into the proximal region of the IL-2 promoter in ...
Tópico(s): Cancer-related gene regulation
2011 - Elsevier BV | Journal of Biological Chemistry
Shuji Tachibanaki, Shin-Ichi Yonetsu, Satoshi Fukaya, Yuki Koshitani, Satoru Kawamura,
Cone photoreceptors show lower light sensitivity and briefer light responses than rod photoreceptors. The light detection signal in these cells is amplified through a phototransduction cascade. The first step of amplification in the cascade is the activation of a GTP-binding protein, transducin (Tr), by light-activated visual pigment (R*). We quantified transducin activation by measuring the binding of GTPγS in purified carp rod and cone membrane preparations with the use of a rapid quench apparatus ...
Tópico(s): Neurobiology and Insect Physiology Research
2012 - Elsevier BV | Journal of Biological Chemistry
Shinji Fukaya, Yuki Matsui, Utano Tomaru, Ai Kawakami, Sayuri Sogo, Toshiyuki Bohgaki, Tatsuya Atsumi, Takao Koike, Masanori Kasahara, Akihiro Ishizu,
TNF-α-converting enzyme (TACE) can cleave transmembrane proteins, such as TNF-α, TNF receptors, and epidermal growth factor receptor (EGFR) ligands, to release the extracellular domains from the cell surface. Recent studies have suggested that overexpression of TACE may be associated with the pathogenesis of inflammation and fibrosis. To determine the roles of TACE in inflammation and fibrosis, TACE transgenic (TACE-Tg) mice, which overexpressed TACE systemically, were generated. As the transgene-derived ...
Tópico(s): Skin and Cellular Biology Research
2012 - Elsevier BV | Laboratory Investigation
Hirobumi Ushijima, Toshio Kawasaki, Toshihide Kamata, Takehito Kodzasa, Hiro Matsuda, Toshio Fukaya, Yuki Fujii, Fujio Mizukami,
Abstract Electrochemical properties and third harmonic generation (THG) properties of bis(1,2-disubstituted-1,2-ethylenedithiolato)metal complexes were examined. This is the first time to report the THG properties of evaporated thin films of these complexes. Nonlinear optical (NLO) properties of these complexes in solutions were already reported by other research groups. It is notable that the title complexes show relatively large THG properties in both solid state and solution. It seems that the ...
Tópico(s): Photochromic and Fluorescence Chemistry
1996 - | Molecular crystals and liquid crystals science technology. Section A, Molecular crystals and liquid crystals/Molecular crystals and liquid crystals science and technology. Section A, Molecular crystals and liquid crystals
Yasushi Kamaya, Yuki Fukaya, Kyoji Suzuki,
The acute immobilization toxicity of benzoic acids substituted with hydroxyl and/or methoxyl groups on the aromatic ring was determined for the freshwater crustacean Daphnia magna under neutralized condition (initial pH: 7.45 ± 0.05). Toxicity, expressed as EC50 value, varied depending largely on the number and position of phenolic hydroxyl groups. Especially, benzoic acids with ortho-substituted hydroxyl groups were more toxic than benzoic acids with meta- and/or para-substituted hydroxyl groups. ...
Tópico(s): Environmental Chemistry and Analysis
2004 - Elsevier BV | Chemosphere
Akiyoshi Higo, Atsuko Isu, Yuki Fukaya, Toru Hisabori,
In the last decade, many studies have been conducted to employ genetically engineered cyanobacteria in the production of various metabolites. However, the lack of a strict gene regulation system in cyanobacteria has hampered these attempts. The filamentous cyanobacterium Anabaena sp. PCC 7120 performs both nitrogen and carbon fixation and is, therefore, a good candidate organism for such production. To employ Anabaena cells for this purpose, we intended to develop artificial gene regulation systems ...
Tópico(s): Biocrusts and Microbial Ecology
2015 - Oxford University Press | Plant and Cell Physiology
Akiyoshi Higo, Atsuko Isu, Yuki Fukaya, Toru Hisabori,
In recent years, studies on the development of gene regulation tools in cyanobacteria have been extensively conducted toward efficient production of valuable chemicals. However, there is considerable scope for improving the economic feasibility of production. To improve a recently reported gene induction system using anhydrotetracycline (aTc)–TetR and an endogenous gene repression system using small antisense RNA in the filamentous nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120 (Anabaena), we ...
Tópico(s): Photosynthetic Processes and Mechanisms
2016 - American Chemical Society | ACS Synthetic Biology
Ryuta Ono, Wataru Ike, Yuki Fukaya,
Toyota Safety Sense is a safety system package developed to help drivers avoid accident types with a high frequency of occurrence. This paper deals with pre-collision system which forms the core of Toyota Safety Sense, especially Toyota Safety Sense P which uses a combined sensor configuration consisting of a monocular camera paired with millimeter wave radar, in order to achieve both high recognition performance and reliability. The use of a wide-angle monocular camera, millimeter wave radar integrated ...
Tópico(s): Risk and Safety Analysis
2016 - | SAE technical papers on CD-ROM/SAE technical paper series
Akiyoshi Higo, Atsuko Isu, Yuki Fukaya, Shigeki Ehira, Toru Hisabori,
Anabaena sp. PCC 7120 (A. 7120) is a heterocyst-forming multicellular cyanobacterium that performs nitrogen fixation. This cyanobacterium has been extensively studied as a model for multicellularity in prokaryotic cells. We have been interested in photosynthetic production of nitrogenous compounds using A. 7120. However, the lack of efficient gene repression tools has limited its usefulness. We originally developed an artificial endogenous gene repression method in this cyanobacterium using small ...
Tópico(s): Microbial Fuel Cells and Bioremediation
2017 - Oxford University Press | Plant and Cell Physiology
Akiyoshi Higo, Atsuko Isu, Yuki Fukaya, Toru Hisabori,
In the last decade, much progress has been made in the photosynthetic production of valuable products using unicellular cyanobacteria. However, production of some products requires dark, anaerobic incubation, which prevents practical applications using these organisms. Anabaena sp. PCC 7120 (A. 7120) is a heterocyst-forming multicellular cyanobacterium that is easy to manipulate genetically. Upon nitrogen step-down, this strain differentiates heterocysts that retain micro-oxic conditions for nitrogen ...
Tópico(s): Algal biology and biofuel production
2017 - Oxford University Press | Plant and Cell Physiology
Keisuke Yoshida, Ayaka Hara, Kazunori Sugiura, Yuki Fukaya, Toru Hisabori,
Thiol-based redox regulation is central to adjusting chloroplast functions under varying light conditions. A redox cascade via the ferredoxin-thioredoxin reductase (FTR)/thioredoxin (Trx) pathway has been well recognized to mediate the light-responsive reductive control of target proteins; however, the molecular basis for reoxidizing its targets in the dark remains unidentified. Here, we report a mechanism of oxidative thiol modulation in chloroplasts. We biochemically characterized a chloroplast ...
Tópico(s): Mitochondrial Function and Pathology
2018 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Kazunori Sugiura, Yuichi Yokochi, Nae Fu, Yuki Fukaya, Keisuke Yoshida, Shoko Mihara, Toru Hisabori,
Thiol-based redox regulation via ferredoxin-thioredoxin (Trx) reductase/Trx controls various functions in chloroplasts in response to light/dark changes. Trx is a key factor of this regulatory system, and five Trx subtypes, including 10 isoforms, have been identified as chloroplast-localized forms in Arabidopsis thaliana. These subtypes display distinct target selectivity, and, consequently, they form a complicated redox regulation network in chloroplasts. In this study, we developed a FRET-based ...
Tópico(s): Metal-Catalyzed Oxygenation Mechanisms
2019 - Elsevier BV | Journal of Biological Chemistry
Yuki Fukaya, Miho Takemura, Takashi Koyanagi, Takashi Maoka, Kazutoshi Shindo, Norihiko Misawa,
There are many reports about carotenoid-producing bacteria and carotenoid biosynthesis genes. In databases for Pseudomonas genome sequences, there are genes homologous to carotenoid biosynthesis genes, but the function of these genes in Pseudomonas has not been elucidated. In this study, we cloned the carotenoid biosynthesis genes from a Pseudomonas sp. strain, named Akiakane, which was isolated from the excrement of the Autumn Darter dragonfly. Using an Escherichia coli functional expression system, ...
Tópico(s): Plant biochemistry and biosynthesis
2017 - Oxford University Press | Bioscience Biotechnology and Biochemistry
Yoshihide Fukaya, Yuki Obama, Shu Hotta, Takeshi Yamao,
We have fabricated diffraction gratings of parylene by evaporation polymerization. The parylene film was deposited onto a commercially available diffraction grating used as a template and transfer-printed onto a substrate. A well-defined periodic structure was replicated on the parylene film surface and its period was in good agreement with that of the template. For future light-emitting device application, we laminated a parylene grating with organic semiconductor crystals and investigated their ...
Tópico(s): Photonic and Optical Devices
2013 - Institute of Physics | Japanese Journal of Applied Physics
Siti Rahmah Aid, Shuhei Hara, Yusuke Shigenaga, Takumi Fukaya, Yūki Tanaka, Satoru Matsumoto, G. Fuse, Susumu Sakuragi,
MOSFETs scaling-down is an effective way to attain high-performance CMOS operating with lower power and leakage current. However, short channel effects have become a serious problem due to the shortening of channel length. One of the promising methods to suppress this problem is by forming a shallow, highly doped and activated source/drain extension region. Fabricating ultra shallow p + /n junction is difficult due to the channeling of boron ions and anomalous boron diffusion during fabrication processes. ...
Tópico(s): Thin-Film Transistor Technologies
2013 - Institute of Physics | Japanese Journal of Applied Physics
Ryota Fukaya, Himawari Hirai, Hirokazu Sakamoto, Yuki Hashimotodani, Kenzo Hirose, Takeshi Sakaba,
Presynaptic long-term potentiation (LTP) is thought to play an important role in learning and memory. However, the underlying mechanism remains elusive because of the difficulty of direct recording during LTP. Hippocampal mossy fiber synapses exhibit pronounced LTP of transmitter release after tetanic stimulation and have been used as a model of presynaptic LTP. Here, we induced LTP by optogenetic tools and applied direct presynaptic patch-clamp recordings. The action potential waveform and evoked ...
Tópico(s): Neural dynamics and brain function
2023 - American Association for the Advancement of Science | Science Advances
Y. Okimoto, T. Egawa, Ryo Fukaya, Y. Matsubara, Yuki Yamada, Nao Yamaya, T. Ishikawa, Ken Onda, Shin‐ya Koshihara, Hiroki Taniguchi, Mitsuru Itoh, Akira Isayama, T. Sasagawa,
Ultrafast optical responses were investigated using a fs laser system on a layered perovskite-type cobalt oxide, La1.5Sr0.5CoO4 with a checkerboard-type charge ordering. To investigate the spatial variation of the photoexcited state, we measured the transient changes of three independent optical quantities, i.e., transmittance, reflectivity, and backside reflectivity, as well as their fluence dependence at 0.25 eV. Using the numerical calculations based on Maxwell's equations, we calculated not ...
Tópico(s): Advanced Condensed Matter Physics
2013 - Physical Society of Japan | Journal of the Physical Society of Japan
Kiminori Nakamura, Yuki Yokoi, Rie Fukaya, Shuya Ohira, Ryuga Shinozaki, Takuto Nishida, Mani Kikuchi, Tokiyoshi Ayabe,
Paneth cells contribute to intestinal innate immunity by sensing bacteria and secreting -defensin. In ICR mice, alpha-defensin termed cryptdin (Crp) in Paneth cells consists of six major isoforms, Crp1 to 6. Despite accumulating evidences that alpha-defensin functions in controlling the intestinal microbiota, topographical localization of Paneth cells in the small intestine in relation to functions of alpha-defensin remains to be determined. In this study, we examined the expression level of mRNA ...
Tópico(s): Pediatric health and respiratory diseases
2020 - Frontiers Media | Frontiers in Immunology
Shinji Ishikawa, Yuka Ito, Yuki Sato, Y. Fukaya, Misa Takahashi, Hiromichi Morikawa, Norikuni Ohtake, Takuji Ohyama, Kuni Sueyoshi,
A high affinity transport system (HATS) for nitrate in plants is operated by a two-component NRT2/NAR2 transport system. However, the regulation and localization of NRT2 and NAR2 at protein level are largely unknown and especially so in crop plant species. In this study with barley (Hordeum vulgare), membrane localization, protein expression in the roots, and a direct protein-protein interaction of HvNRT2 and HvNAR2 proteins were investigated. Immunochemical analysis showed that both HvNRT2 and HvNAR2 ...
Tópico(s): Plant Micronutrient Interactions and Effects
2009 - Japanese Society for Plant Biotechnology | Plant Biotechnology
Michiro Kiryu, Shinichi Niwano, Hiroe Niwano, Jun Kishihara, Yuki Aoyama, Hidehira Fukaya, Y Masaki, T. Izumi,
Remodelling of the extracellular matrix (ECM) plays an important role in the production of arrhythmogenic substrate for atrial fibrillation (AF), and is considered to be promoted by the connective tissue growth factor (CTGF).Our objective was to assess the relationship between CTGF and ECM synthesis, and the effect of olmesartan on these processes. Methods and resultsFifteen canine AF models were produced by rapid atrial stimulation.They were divided into three groups: pacing control (n ¼ 5): 6-week ...
Tópico(s): Periodontal Regeneration and Treatments
2012 - Oxford University Press | EP Europace
Yuki Nakatani, Tetsuya Fukaya, Shigenobu Kishino, Jun Ogawa,
To produce tomato juice with health-promoting functions, lactic acid bacteria (LAB) capable of converting l-glutamic acid in tomatoes into γ-aminobutyric acid (GABA) was screened from LAB stocks isolated from Japanese pickles. Lactiplantibacillus plantarum KB1253 was selected as the highest GABA producer among 74 strains of LAB stocks. gad gene expression and glutamic acid decarboxylation activity increased at low pH (3.0-3.5), whereas the growth decreased. Under optimal reaction conditions using resting ...
Tópico(s): Medicinal Plants and Neuroprotection
2022 - Elsevier BV | Journal of Bioscience and Bioengineering
Sari Fukaya, Hiroyuki Kanzaki, Yutaka Miyamoto, Yuki Yamaguchi, Yoshiki Nakamura,
•IGF-1 was injected unilaterally into the mandibular condylar cavity in mice. •Local IGF-1 injection induced a lateral shift of the mandible. •Local injection into the condylar cavity induced endochondral growth and ossification. •Chondrogenic and osteoblastic gene expression was upregulated. •No systemic effects were found. Introduction The purpose of this study was to investigate whether a local unilateral IGF-1 injection into the mandibular condylar cavity can induce unilateral endochondral mandibular ...
Tópico(s): Craniofacial Disorders and Treatments
2017 - Elsevier BV | American Journal of Orthodontics and Dentofacial Orthopedics
Koichiro Ueki, Akinori Moroi, Kunio Yoshizawa, Asami Hotta, Takamitsu Tsutsui, Kenichi Fukaya, Ryota Hiraide, Akihiro Takayama, Tatsuta Tsunoda, Yuki Saito,
The purpose of this study was to examine skeletal stability and plate breakage after sagittal split ramus osteotomy (SSRO) with the mono-cortical plate fixation, bi-cortical plate fixation, and hybrid fixation techniques using absorbable plates and screws. A total of 76 Japanese patients diagnosed with mandibular prognathism with and without maxillary deformity were divided into 3 groups randomly. A total of 28 patients underwent SSRO with mono-cortical plate fixation, 23 underwent SSRO with bi-cortical ...
Tópico(s): Craniofacial Disorders and Treatments
2016 - Elsevier BV | Journal of Cranio-Maxillofacial Surgery
Koichiro Ueki, Kunio Yoshizawa, Akinori Moroi, Asami Hotta, Takamitsu Tsutsui, Kenichi Fukaya, Ryota Hiraide, Akihiro Takayama, Tatsuya Tsunoda, Yuki Saito, Nana Baba, Momoko Sato,
The purpose of this study was to examine the skeletal stability of mandibular advancement after sagittal split ramus osteotomy (SSRO) with modified hybrid fixation using absorbable plates and screws.A total of 54 Japanese patients were enrolled in this study. Of them, 23 who were diagnosed with mandibular prognathism underwent setback surgery with bi-cortical plate fixation using absorbable plates and screws (setback group). Another 23 who were diagnosed with mandibular retrognathism underwent advancement ...
Tópico(s): Craniofacial Disorders and Treatments
2017 - Elsevier BV | Journal of Cranio-Maxillofacial Surgery
Ryo Nishinarita, Shinichi Niwano, Hidehira Fukaya, Jun Oikawa, Takeru Nabeta, Gen Matsuura, Yuki Arakawa, Shuhei Kobayashi, Yuki Shirakawa, Ai Horiguchi, Hironori Nakamura, Naruya Ishizue, Jun Kishihara, Akira Satoh, Junya Ako,
The relationship between atrial high-rate episode (AHRE) burden (i.e., the frequency of atrial tachyarrhythmia) and heart failure (HF) risk is unclear. We hypothesized that new-onset and higher burden of AHRE are associated with HF.
Tópico(s): Atrial Fibrillation Management and Outcomes
2019 - Japanese Circulation Society | Circulation Journal
Akinori Moroi, Kunio Yoshizawa, Takamitsu Tsutsui, Yuriko Saida, Asami Hotta, Kenichi Fukaya, Ryota Hiraide, Akihiro Takayama, Tatsuya Tunoda, Yuki Saito, Koichiro Ueki,
The purpose of this study was to evaluate the effects of Le Fort I osteotomy on nasal septum deviation and differences in left and right airway sizes, and to determine whether the nasal septum was affected by differences in the direction of movement. Forty patients underwent conventional Le Fort I osteotomy, and computed tomography (CT) was performed preoperatively, and 1 week and 1 year postoperatively. The nasal septum angle and airway area were measured at the anterior, middle, and posterior positions ...
Tópico(s): Head and Neck Surgical Oncology
2016 - Elsevier BV | Journal of Cranio-Maxillofacial Surgery
Takumi Motoya, Masahiro Umezawa, Aoi Saito, Keiko Goto, Ikuko Doi, Setsuko Fukaya, Noriko Nagata, Yoshiaki Ikeda, Kaori Okayama, Jumpei Aso, Yuki Matsushima, Taisei Ishioka, Akihide Ryo, Nobuya Sasaki, Kazuhiko Katayama, Hirokazu Kimura,
Human norovirus (HuNoV) is the major cause of viral acute gastroenteritis for all age groups in various countries. HuNoV GII in particular accounted for the majority of norovirus outbreaks, among which GII.4 caused repeated outbreaks for a long time. Besides GII.4, other norovirus genotypes, GII.2, GII.6, and GII.17, have also been prevalent in various contexts in recent years, but few detailed epidemiological studies of them have been performed and are poorly understood. We thus conducted an epidemiological ...
Tópico(s): Viral Infections and Immunology Research
2019 - BioMed Central | Gut Pathogens
Kumiko Kondo, Yu Takeyama, Ei-Ichiro Sunamura, Yuka Madoka, Yuki Fukaya, Atsuko Isu, Toru Hisabori,
F1 is a soluble part of FoF1-ATP synthase and performs a catalytic process of ATP hydrolysis and synthesis. The γ subunit, which is the rotary shaft of F1 motor, is composed of N-terminal and C-terminal helices domains, and a protruding Rossman-fold domain located between the two major helices parts. The N-terminal and C-terminal helices domains of γ assemble into an antiparallel coiled-coil structure, and are almost embedded into the stator ring composed of α3β3 hexamer of the F1 molecule. Cyanobacterial ...
Tópico(s): Photosynthetic Processes and Mechanisms
2018 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Bioenergetics
Shuhei Kobayashi, Hidehira Fukaya, Jun Oikawa, Daiki Saito, Tetsuro Sato, Gen Matsuura, Yuki Arakawa, Yuki Shirakawa, Ryo Nishinarita, Ai Horiguchi, Naruya Ishizue, Jun Kishihara, Shinichi Niwano, Junya Ako,
Tópico(s): Cardiac pacing and defibrillation studies
2020 - Springer Science+Business Media | Journal of Interventional Cardiac Electrophysiology