Kiyotaka Shiba, Tsuyoshi Hatada, Yu Takahashi, Takeshi Noda,
... this author on: Oxford Academic PubMed Google Scholar Yuki Takahashi, Yuki Takahashi †Department of Cell Biology, Cancer Institute, Japanese Foundation ...
Tópico(s): RNA modifications and cancer
2002 - Oxford University Press | The Journal of Biochemistry
Yuki Tamura, Yutaka Matsunaga, Hiroyuki Masuda, Yumiko Takahashi, Yuki Takahashi, Shin Terada, Daisuke Hoshino, Hideo Hatta,
A recent study demonstrated that heat stress induces mitochondrial biogenesis in C2C12 myotubes, thereby implying that heat stress may be an effective treatment to enhance endurance training-induced mitochondrial adaptations in skeletal muscle. However, whether heat stress actually induces mitochondrial adaptations in skeletal muscle in vivo is unclear. In the present study, we report the novel findings that 1) whole body heat stress produced by exposure of ICR mice to a hot environment (40°C, 30 ...
Tópico(s): Exercise and Physiological Responses
2014 - American Physiological Society | AJP Regulatory Integrative and Comparative Physiology
Haruo Usuda, Shimpei Watanabe, Masatoshi Saito, Hideyuki Ikeda, Shota Koshinami, Shinichi Sato, Gabrielle C. Musk, Erin L. Fee, Sean Carter, Yusaku Kumagai, Tsukasa Takahashi, Yuki Takahashi, Shinichi Kawamura, Takushi Hanita, Shigeo Kure, Nobuo Yaegashi, John P. Newnham, Matthew W. Kemp,
Background Ex vivo uterine environment therapy is an experimental intensive care strategy for extremely preterm infants born between 21 and 24 weeks of gestation. Gas exchange is performed by membranous oxygenators connected by catheters to the umbilical vessels. The fetus is submerged in a bath of synthetic amniotic fluid. The lungs remain fluid filled, and pulmonary respiration does not occur. Intrauterine inflammation is strongly associated with extremely preterm birth and fetal injury. At present, ...
Tópico(s): Neonatal and fetal brain pathology
2020 - Elsevier BV | American Journal of Obstetrics and Gynecology
Daiki Nomura, Masaaki Saito, Yosuke Takahashi, Yuki Takahashi, Yoshinobu Takakura, Makiya Nishikawa,
Self-gelling DNA hydrogels with cytosine-phosphate-guanine (CpG) motifs have been shown to exhibit high potency as vaccine adjuvants. However, their oral use is limited because of their thermodynamic and chemical instability in the gastrointestinal tract. In this study, we aimed to develop DNA hydrogel microspheres (Dgel-MS) coated with chitosan to improve their stability. Chitosan-coated Dgel-MS (Cs-Dgel-MS) was prepared by emulsifying Dgel to obtain the D-gel core, followed by mixing with microemulsions ...
Tópico(s): Immunotherapy and Immune Responses
2018 - Elsevier BV | International Journal of Pharmaceutics
Yuka Umeki, Kohta Mohri, Yohji Kawasaki, Hiroshi Watanabe, Rei Takahashi, Yuki Takahashi, Yoshinobu Takakura, Makiya Nishikawa,
Previous studies indicate that immunostimulatory DNA‐based injectable hydrogels harboring unmethylated cytosine‐phosphate‐guanine (CpG) dinucleotides meet the requirements of an effective antigen delivery system, including safety, biodegradability, ease of administration, and stimulation of the innate immune system. However, rapid release of the model antigen ovalbumin (OVA) from the hydrogel limits its potential. Here, the aim is to achieve sustained OVA release from a DNA hydrogel through cationization ...
Tópico(s): Immune Cell Function and Interaction
2015 - Wiley | Advanced Functional Materials
Makiya Nishikawa, Kohei Ogawa, Yuka Umeki, Kohta Mohri, Yohji Kawasaki, Hiroshi Watanabe, Natsuki Takahashi, Eri Kusuki, Rei Takahashi, Yuki Takahashi, Yoshinobu Takakura,
DNA nanotechnology-based nanosystems and macrosystems have attracted much attention in the biomedical research field. The nature of DNA endows these systems with biodegradable, biocompatible, and immunomodulatory properties. Here, we present an injectable hydrogel system that consists only of chemically synthesized short DNA strands, water, and salts. Several preparations of polypod-like structured DNA, or polypodna, were designed, including tri-, tetra-, penta- and hexapodna, as the building blocks ...
Tópico(s): DNA and Nucleic Acid Chemistry
2014 - Elsevier BV | Journal of Controlled Release
Makiya Nishikawa, Ayumi Tamada, Kenji Hyoudou, Yukari Umeyama, Yuki Takahashi, Yuki Kobayashi, Hitomi Kumai, Emi Ishida, František Štaud, Yoshiyuki Yabe, Yoshinobu Takakura, Fumiyoshi Yamashita, Mitsuru Hashida,
Tópico(s): Coagulation, Bradykinin, Polyphosphates, and Angioedema
2004 - Springer Science+Business Media | Clinical & Experimental Metastasis
Akira Kato, Hiroyuki Takahashi, Yuki Takahashi, Hitoshi Matsushime,
Cyclin-dependent kinase (Cdk) inhibitory proteins are involved in cell cycle arrest induced by antiproliferating factors or chemicals. High cell density also induces cell cycle arrest in which the genomic DNA is unreplicated, even in the presence of a mitotic dose of growth factors; this is termed contact inhibition. Although the cell cycle of the rat fibroblast cell line, 3Y1, was arrested in quiescence by contact inhibition, the Cdk4 bound to its regulatory subunit, cyclin D1 or D3. However, these ...
Tópico(s): DNA Repair Mechanisms
1997 - Elsevier BV | Journal of Biological Chemistry
Yuko Nitahara‐Kasahara, Shuji Mizumoto, Yukiko Inoue, Shota Saka, Guillermo Posadas-Herrera, Aki Nakamura-Takahashi, Yuki Takahashi, Ayana Hashimoto, Kohei Konishi, Shinji Miyata, Chiaki Masuda, Emi Matsumoto, Yasunobu Maruoka, Takahiro Yoshizawa, Toshiki Tanase, Takayoshi Inoue, Shuhei Yamada, Y. Nomura, Shin’ichi Takeda, Atsushi Watanabe, Tomoki Kosho, Takashi Okada,
ABSTRACT Musculocontractural Ehlers-Danlos syndrome (mcEDS) is caused by generalized depletion of dermatan sulfate (DS) due to biallelic pathogenic variants in CHST14 encoding dermatan 4-O-sulfotransferase 1 (D4ST1) (mcEDS-CHST14). Here, we generated mouse models for mcEDS-CHST14 carrying homozygous mutations (1 bp deletion or 6 bp insertion/10 bp deletion) in Chst14 through CRISPR/Cas9 genome engineering to overcome perinatal lethality in conventional Chst14-deleted knockout mice. DS depletion was detected ...
Tópico(s): Glycosylation and Glycoproteins Research
2021 - The Company of Biologists | Disease Models & Mechanisms
Yosuke Takahashi, Tatsuoki Maezawa, Yuki Araie, Yuki Takahashi, Yoshinobu Takakura, Makiya Nishikawa,
Cytosine-phosphate-guanine (CpG) DNA is known to increase the potency of vaccines. Here, in vitro and in vivo stimulation of toll-like receptor 9 by CpG DNA incorporated into polypod-like DNA nanostructures was evaluated by measuring the levels of tumor necrosis factor alpha released from macrophage-like RAW 264.7 cells and plasma interleukin (IL)-12p40 in vivo following intravenous injection into mice. Phosphodiester CpG1668 was selected as the CpG DNA, and tripodna and hexapodna, which were CpG1668- ...
Tópico(s): Immunotherapy and Immune Responses
2017 - Elsevier BV | Journal of Pharmaceutical Sciences
Tsukasa Takahashi, Erin L. Fee, Yuki Takahashi, Masatoshi Saito, Nobuo Yaegashi, Haruo Usuda, Lucy L. Furfaro, Sean Carter, Augusto F. Schmidt, John P. Newnham, Alan H. Jobe, Matthew W. Kemp,
Antenatal corticosteroid therapy is a standard of care for women at imminent risk of preterm labor. However, the optimal (maximum benefit and minimal risk of side effects) antenatal corticosteroid dosing strategy remains unclear. Although conveying overall benefit when given to the right patient at the right time, antenatal corticosteroid treatment efficacy is highly variable and is not risk-free. Building on earlier findings, we hypothesized that when administered in combination with slow-release ...
Tópico(s): Preterm Birth and Chorioamnionitis
2021 - Elsevier BV | American Journal of Obstetrics and Gynecology
Ryuhei Nishiyabu, Yuki Takahashi, Taro Yabuki, Shoji Gommori, Yuki Yamamoto, Hiroaki Kitagishi, Yuji Kubo,
The self-assembly of polyvinyl alcohol (PVA) and benzene-1,4-diboronic acid (DBA) is employed as a sol-gel method for one-step fabrication of hydrogel coatings with versatile functionalities. A mixture of PVA and DBA in aqueous ethanol is prepared as a coating agent. The long pot life of the mixture allows for the coating of a wide range of materials with hydrogel films by simple cast- and dip-coating techniques. The resultant films show negligible dissolution in water and the intrinsic hydrophilicity ...
Tópico(s): Electrospun Nanofibers in Biomedical Applications
2019 - Royal Society of Chemistry | RSC Advances
Kohta Mohri, Eri Kusuki, Shozo Ohtsuki, Natsuki Takahashi, Masayuki Endo, Kumi Hidaka, Hiroshi Sugiyama, Yuki Takahashi, Yoshinobu Takakura, Makiya Nishikawa,
DNA dendrimers consisting of several branched DNA units connected to each other using DNA ligase were quite effective for the delivery of immunostimulatory CpG DNA to immune cells. Therapeutic application of such DNA dendrimers, however, is hampered by the use of the ligase. Here, we report that self-assembling DNA dendrimers with high immunostimulatory potency can be prepared without DNA ligases. Annealing of DNA consisting of DNA units with elongated adhesive ends resulted in the formation of ...
Tópico(s): Advanced biosensing and bioanalysis techniques
2015 - American Chemical Society | Biomacromolecules
Kohta Mohri, Natsuki Takahashi, Makiya Nishikawa, Eri Kusuki, Tomoki Shiomi, Yuki Takahashi, Yoshinobu Takakura,
The immunostimulatory activity of phosphodiester DNA containing unmethylated cytosine-guanine (CpG) dinucleotides can be increased by converting it into branched structures. These structures could be stabilized by ligating the 5'- and 3'-ends to form a closed loop with no terminal ends. To further increase the ability of branched DNA assemblies to induce cytokines, a series of tetrapod-like structured DNA, or tetrapodna, were designed using four 48-base oligodeoxynucleotides (ODNs). All these preparations ...
Tópico(s): Immune Cell Function and Interaction
2012 - Elsevier BV | Journal of Controlled Release
K. Nakayama, T. Sato, Kensei Terashima, H. Matsui, T. Takahashi, Masato Kubota, Kanta Ono, Terukazu Nishizaki, Yuki Takahashi, Norio Kobayashi,
We have performed high-resolution angle-resolved photoemission spectroscopy on $\mathrm{Y}{\mathrm{Ba}}_{2}{\mathrm{Cu}}_{3}{\mathrm{O}}_{7\ensuremath{-}\ensuremath{\delta}}$ (Y123; $\ensuremath{\delta}=0.06$; ${T}_{\mathrm{c}}=92\phantom{\rule{0.3em}{0ex}}\mathrm{K}$). By accurately determining the Fermi surface and energy band dispersion, we solve long-standing controversial issues as to the anomalous electronic states of Y-based high-${T}_{\mathrm{c}}$ cuprates. We unambiguously identified surface-bilayer- ...
Tópico(s): Iron-based superconductors research
2007 - American Physical Society | Physical Review B
Kohta Mohri, Makiya Nishikawa, Natsuki Takahashi, Tomoki Shiomi, Nao Matsuoka, Kohei Ogawa, Masayuki Endo, Kumi Hidaka, Hiroshi Sugiyama, Yuki Takahashi, Yoshinobu Takakura,
The immunostimulatory activity of phosphodiester DNA containing unmethylated cytosine-phosphate-guanine (CpG) dinucleotides, or CpG motifs, was significantly increased by the formation of Y-, X-, or dendrimer-like multibranched shape. These results suggest the possibility that the activity of CpG DNA is a function of the structural properties of branched DNA assemblies. To elucidate the relationship between them, we have designed and developed nanosized DNA assemblies in polypod-like structures (polypod- ...
Tópico(s): DNA and Nucleic Acid Chemistry
2012 - American Chemical Society | ACS Nano
Yuki Takahashi, M Kalafatis, JP Girma, K Sewerin, L.-O. Andersson, Dominique Meyer,
Abstract Factor VIII (F.VIII) was tested for its ability to bind in solid phase system to von Willebrand Factor (vWF) or fragments obtained with Staphylococcus aureus V-8 protease, ie, SpIII (N-terminal), SpI (central), and SpII (C-terminal). Bound F.VIII was estimated in situ by clotting and chromogenic assays. F.VIII bound in a dose-dependent manner to immobilized vWF and SpIII but not to SpII or SpI. Binding was inhibited by 0.25 mol/L CaCl2 as well as by an excess of vWF or SpIII. Accordingly, immobilized ...
Tópico(s): Coagulation, Bradykinin, Polyphosphates, and Angioedema
1987 - Elsevier BV | Blood
Takeshi Ogasawara, Yuki Takahashi, K. Kanbara, Y. Kubota, K. Yasohama, K. Yasuköchi,
Theoretical studies are presented for transient field losses in multifilamentary composite conductors carrying a dc transport current. The analysis of the total loss is split into separate calculations of the magnetization loss and the loss due to the dynamic resistance. Analytical expressions are derived for a field change of trapezoidal shape. The parameter which characterizes the total loss is a dimensionless sweep rate of the external field β = τḂe/Bp, where τ is the relaxation time of the eddy ...
Tópico(s): Mechanical stress and fatigue analysis
1980 - Elsevier BV | Cryogenics
Takeshi Ogasawara, Yuki Takahashi, K. Kanbara, Y. Kubota, K. Yasohama, K. Yasuköchi,
The influence of dc transport currents on alternating-field hysteresis loss was investigated for a single core superconducting wire. The configuration of the sample coil simulates a tight solenoidal winding of superconducting magnets, and the field is a small transverse alternating field applied on top of a large dc bias. The losses were determined as a function of transport current and external field from simultaneous measurements of the magnetization and the dynamic resistance. The results were ...
Tópico(s): HVDC Systems and Fault Protection
1979 - Elsevier BV | Cryogenics
Yusuke Kawamoto, You Wu, Yuki Takahashi, Yoshinobu Takakura,
Oligonucleotide-based therapeutics have attracted attention as an emerging modality that includes the modulation of genes and their binding proteins related to diseases, allowing us to take action on previously undruggable targets. Since the late 2010s, the number of oligonucleotide medicines approved for clinical uses has dramatically increased. Various chemistry-based technologies have been developed to improve the therapeutic properties of oligonucleotides, such as chemical modification, conjugation, ...
Tópico(s): DNA and Nucleic Acid Chemistry
2023 - Elsevier BV | Advanced Drug Delivery Reviews
Yuki Takahashi, Yoshinobu Takakura,
Tópico(s): Nanoplatforms for cancer theranostics
2023 - Elsevier BV | Pharmacology & Therapeutics
Ryosuke Matsuo, Yuki Takahashi, Seiichi Watanabe, Satoshi Okabe,
Three-dimensional ZnO/CuO nanoforests (NFRs) were fabricated via the galvanic submerged photosynthesis of crystallites in water at ambient temperature and pressure, and their photoelectrochemical (PEC) properties and applicability to microbial PEC (MPEC) cells were evaluated. ZnO/CuO NFRs irradiated with UV light for 48 h showed a high PEC current of −2.9 ± 1.3 mA/cm2 and hydrogen (H2) generation rate of 0.63 ± 0.29 µmol/cm2/min at 0 V vs. RHE owing to their superior light absorption, large BET specific ...
Tópico(s): Advanced Photocatalysis Techniques
2023 - Elsevier BV | Applied Catalysis B Environment and Energy
Produção Nacional
Revisado por pares
Kajsa Brimdyr, Jeni Stevens, Kristin Svensson, Anna Blair, Cindy Turner‐Maffei, Julie C Grady, Louise Racine Bastarache, Abla al Alfy, Jeannette T. Crenshaw, Elsa Regina Justo Giugliani, Uwe Ewald, Rukhsana Haider, Wibke Jonas, Mike Kagawa, Siri Lilliesköld, Ragnhild Maastrup, Ravae Sinclair, Emma Swift, Yuki Takahashi, Karin Cadwell,
Skin-to-skin contact immediately after birth is recognised as an evidence-based best practice and an acknowledged contributor to improved short- and long-term health outcomes including decreased infant mortality. However, the implementation and definition of skin-to-skin contact is inconsistent in both practice and research studies. This project utilised the World Health Organization guideline process to clarify best practice and improve the consistency of application.The rigorous guideline development ...
Tópico(s): Maternal Mental Health During Pregnancy and Postpartum
2023 - Wiley | Acta Paediatrica
Hideo Tanaka, Tsuyoshi Ogata, Toshiyuki Shibata, Hitomi Nagai, Yuki Takahashi, Masaru Kinoshita, K. Matsubayashi, Sanae HATTORI, Chie Taniguchi,
We aimed to elucidate the range of the incubation period in patients infected with the SARS-CoV-2 Omicron variant in comparison with the Alpha variant. Contact tracing data from three Japanese public health centers (total residents, 1.06 million) collected following the guidelines of the Infectious Diseases Control Law were reviewed for 1589 PCR-confirmed COVID-19 cases diagnosed in January 2022. We identified 77 eligible symptomatic patients for whom the date and setting of transmission were known, ...
Tópico(s): SARS-CoV-2 detection and testing
2022 - Multidisciplinary Digital Publishing Institute | International Journal of Environmental Research and Public Health
Wen Liu, Maki Ota, Mayu Tabushi, Yuki Takahashi, Yoshinobu Takakura,
Allergic rhinitis is caused by a breakdown of the Th1/Th2 balance, in which the allergen-induced Th2 immune response predominates over the Th1 immune response, culminating in IgE-mediated anaphylaxis. In this study, we used small extracellular vesicles (sEVs), cell-derived membrane vesicles with a particle size of 100 nm, as simultaneous delivery carriers for allergens (ovalbumin, OVA) and CpG DNA, an adjuvant that can induce a Th1 immune response, for the treatment of allergic rhinitis. sEVs loaded ...
Tópico(s): Inhalation and Respiratory Drug Delivery
2022 - Elsevier BV | Journal of Controlled Release
Yoshinobu Takakura, Yuki Takahashi,
The enhanced permeability and retention (EPR) effect has been the gold standard in developing drug delivery systems for passive tumor targeting. Although the importance of this concept remains unchanged, some controversies have arisen. In this review, various strategies for tumor targeting using macromolecules and nanoparticles based on the EPR effect are discussed from the viewpoint of pharmacokinetics. Overall, such strategies seek to retain therapeutic material in the blood circulation, which ...
Tópico(s): Nanoparticle-Based Drug Delivery
2022 - Elsevier BV | Journal of Controlled Release
Tsuyoshi Ogata, Hideo Tanaka, Fujiko Irie, Atsushi Hirayama, Yuki Takahashi,
Few studies have assessed incubation periods of the severe acute respiratory syndrome coronavirus 2 Delta variant. This study aimed to elucidate the transmission dynamics, especially the incubation period, for the Delta variant compared with non-Delta strains. We studied unvaccinated coronavirus disease 2019 patients with definite single exposure date from August 2020 to September 2021 in Japan. The incubation periods were calculated and compared by Mann-Whitney U test for Delta (with L452R mutation) ...
Tópico(s): SARS-CoV-2 detection and testing
2022 - Multidisciplinary Digital Publishing Institute | International Journal of Environmental Research and Public Health
Yuki Takahashi, Melbert Jeem, Lihua Zhang, Seiichi Watanabe,
Semiconductor hetero-nanostructures are of great interest for practical industry use. In this report, we demonstrated ZnO/CuO nanoforest (NFRs) fabricated by galvanic submerged photo-synthesis (G-SPSC) method, which utilizes light illumination in pure water without additives. For the first time, we elucidated the origin of its enhanced opto-functional properties. At the hetero-epitaxial interface, ZnO(001)/CuO(001) planes linkage were established, even though with 13.62 – 28.15% local lattice discrepancies ...
Tópico(s): Electronic and Structural Properties of Oxides
2022 - Elsevier BV | Applied Materials Today
Misako Takenaka, Ayane Yabuta, Yuki Takahashi, Yoshinobu Takakura,
Interleukin-4 (IL4), a Th2-type cytokine that can drive M2 macrophage polarization, is expected to be used as an anti-inflammatory therapy agent as M2 polarization of macrophages can ameliorate chronic inflammation. However, several problems, such as the low effectiveness and side effects, have hampered the clinical application. To safely and effectively use IL4, an efficient delivery of IL4 to target cells, macrophages, is necessary. Small extracellular vesicles (sEVs) are promising candidates as ...
Tópico(s): Reproductive System and Pregnancy
2021 - Elsevier BV | Biomaterials
Hideo Tanaka, Atsushi Hirayama, Hitomi Nagai, Chika Shirai, Yuki Takahashi, Hiroto Shinomiya, Chie Taniguchi, Tsuyoshi Ogata,
To assess the relative transmissibility of the SARS-CoV-2 Alpha variant compared to the pre-existing SARS-CoV-2 in Japan, we performed a cross-sectional study to determine the secondary attack rate of COVID-19 in household contacts before and after the Alpha variant became dominant in Osaka. We accessed 290 household contacts whose index cases were diagnosed between 1 and 20 December 2020 (the third epidemic group), at a time when Osaka was free of the Alpha variant. We also accessed 398 household ...
Tópico(s): Viral Infections and Outbreaks Research
2021 - Multidisciplinary Digital Publishing Institute | International Journal of Environmental Research and Public Health