David L. Nelson, A. Herbet, Yves Pétillot, L. Pichat, J. Głowiński, M. Hamon,
Abstract A high‐affinity (K d = 5.9 nM) specific binding site for [ 3 H]harmaline was detected in membranes from rat and bovine brains. Studies of the regional and subcellular distributions of this binding indicated its close association with monoamine oxidase type A activity (MAO A) measured with [ 3 H]serotonin ([ 3 H]5‐HT) as the substrate. Maximal binding capacity and MAO A activity were found in mitochondrial enriched fractions. Mitochondria of synaptosomal or extra‐synaptosomal origin exhibited very ...
Tópico(s): Nicotinic Acetylcholine Receptors Study
1979 - Wiley | Journal of Neurochemistry
Céline Ortega, Amaury Herbet, Sophie Richard, Nathalie Kersual, Narciso Costa, André Pèlegrin, Frédéric Ducancel, Jean-Yves Couraud, Isabelle Navarro‐Teulon, Didier Boquet,
Prescription of therapeutic antibodies has radically modified the prognosis of some important diseases. However, the very high cost of these new drugs is a problem for public health organizations, which require assessment of the effectiveness of the antibody for each patient before beginning or during the treatment. In vivo immunoimaging is particularly well adapted to meet this demand. However, full-length antibodies are unsuitable for in vivo imaging due to their persistence in the serum and must ...
Tópico(s): Toxin Mechanisms and Immunotoxins
2012 - Elsevier BV | Journal of Immunological Methods
Aurélie Borrull, Bertrand Allard, Anne Wijkhuisen, Amaury Herbet, Patricia Lamourette, Wided Birouk, Denis Leiber, Zahra Tanfin, Frédéric Ducancel, Didier Boquet, Jean-Yves Couraud, Philippe Robin,
Metastatic melanoma is an aggressive cancer with a poor prognostic, and the design of new targeted drugs to treat melanoma is a therapeutic challenge. A promising approach is to produce monoclonal antibodies (mAbs) against the endothelin B receptor (ETB), which is known to be overexpressed in melanoma and to contribute to proliferation, migration and vasculogenic mimicry associated with invasiveness of this cancer.We previously described rendomab-B1, a mAb produced by DNA immunization. It is endowed ...
Tópico(s): Cytokine Signaling Pathways and Interactions
2016 - Landes Bioscience | mAbs
Margaux Lejeune, Murat Cem Köse, Mégane Jassin, Marie‐Jia Gou, Amaury Herbet, Elodie Duray, Gaël Cobraiville, Jacques Foguenne, Didier Boquet, André Gothot, Yves Béguin, Marianne Fillet, Jo Caers,
Despite the recent introduction of next-generation immunotherapeutic agents, multiple myeloma (MM) remains incurable. New strategies targeting MM-specific antigens may result in a more effective therapy by preventing antigen escape, clonal evolution, and tumor resistance. In this work, we adapted an algorithm that integrates proteomic and transcriptomic results of myeloma cells to identify new antigens and possible antigen combinations. We performed cell surface proteomics on 6 myeloma cell lines ...
Tópico(s): Chemokine receptors and signaling
2023 - Wolters Kluwer | HemaSphere