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Minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE)

2008; Nature Portfolio; Volume: 26; Issue: 3 Linguagem: Inglês

10.1038/nbt1391

1546-1696

Eric W. Deutsch, Catherine A. Ball, Jules J. Berman, G. Steven Bova, Alvis Brāzma, Roger E. Bumgarner, David Campbell, Helen C. Causton, Jeffrey H. Christiansen, Fabrice Daian, Delphine Dauga, Duncan Davidson, Grégory Gimenez, Young Ah Goo, Sean M. Grimmond, Thorsten Henrich, Bernhard G. Herrmann, Michael H. Johnson, Martin Korb, Jason C. Mills, Asa Oudes, Helen Parkinson, Laura E. Pascal, Nicolas Pollet, John Quackenbush, Mirana Ramialison, Martin Ringwald, David Salgado, Susanna‐Assunta Sansone, Gavin Sherlock, Christian J. Stoeckert, Jason R. Swedlow, Ronald C. Taylor, Laura S Walashek, Anthony Warford, David G. Wilkinson, Yi Zhou, Leonard I. Zon, Alvin Y. Liu, Lawrence D. True,

Molecular Biology Techniques and Applications

One purpose of the biomedical literature is to report results in sufficient detail that the methods of data collection and analysis can be independently replicated and verified. Here we present reporting guidelines for gene expression localization experiments: the minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE). MISFISHIE is modeled after the Minimum Information About a Microarray Experiment (MIAME) specification for microarray experiments. Both guidelines define what information should be reported without dictating a format for encoding that information. MISFISHIE describes six types of information to be provided for each experiment: experimental design, biomaterials and treatments, reporters, staining, imaging data and image characterizations. This specification has benefited the consortium within which it was developed and is expected to benefit the wider research community. We welcome feedback from the scientific community to help improve our proposal.