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Correlation between the structure and biochemical activities of FtsA, an essential cell division protein of the actin family.

1994; Springer Nature; Volume: 13; Issue: 20 Linguagem: Inglês

10.1002/j.1460-2075.1994.tb06819.x

1460-2075

Manuel Sánchez, Alfonso Valencia, María-José Ferrándiz, Chris Sander, Miguel Vicente,

RNA Research and Splicing

Research Article17 October 1994free access Correlation between the structure and biochemical activities of FtsA, an essential cell division protein of the actin family. M. Sánchez M. Sánchez Departamento de Biología Celular y del Desarrollo, CIB, Consejo Superior de Investigaciones Científicas, Madrid, Spain. Search for more papers by this author A. Valencia A. Valencia Departamento de Biología Celular y del Desarrollo, CIB, Consejo Superior de Investigaciones Científicas, Madrid, Spain. Search for more papers by this author M.J. Ferrándiz M.J. Ferrándiz Departamento de Biología Celular y del Desarrollo, CIB, Consejo Superior de Investigaciones Científicas, Madrid, Spain. Search for more papers by this author C. Sander C. Sander Departamento de Biología Celular y del Desarrollo, CIB, Consejo Superior de Investigaciones Científicas, Madrid, Spain. Search for more papers by this author M. Vicente M. Vicente Departamento de Biología Celular y del Desarrollo, CIB, Consejo Superior de Investigaciones Científicas, Madrid, Spain. Search for more papers by this author M. Sánchez M. Sánchez Departamento de Biología Celular y del Desarrollo, CIB, Consejo Superior de Investigaciones Científicas, Madrid, Spain. Search for more papers by this author A. Valencia A. Valencia Departamento de Biología Celular y del Desarrollo, CIB, Consejo Superior de Investigaciones Científicas, Madrid, Spain. Search for more papers by this author M.J. Ferrándiz M.J. Ferrándiz Departamento de Biología Celular y del Desarrollo, CIB, Consejo Superior de Investigaciones Científicas, Madrid, Spain. Search for more papers by this author C. Sander C. Sander Departamento de Biología Celular y del Desarrollo, CIB, Consejo Superior de Investigaciones Científicas, Madrid, Spain. Search for more papers by this author M. Vicente M. Vicente Departamento de Biología Celular y del Desarrollo, CIB, Consejo Superior de Investigaciones Científicas, Madrid, Spain. Search for more papers by this author Author Information M. Sánchez1, A. Valencia1, M.J. Ferrándiz1, C. Sander1 and M. Vicente1 1Departamento de Biología Celular y del Desarrollo, CIB, Consejo Superior de Investigaciones Científicas, Madrid, Spain. The EMBO Journal (1994)13:4919-4925https://doi.org/10.1002/j.1460-2075.1994.tb06819.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Cell division protein FtsA, predicted to belong to the actin family, is present in different cell compartments depending on its phosphorylation state. The FtsA fraction isolated from the cytoplasm is phosphorylated and capable of binding ATP, while the membrane-bound form is unphosphorylated and does not bind ATP. A variant of the protein FtsA102, in which the nucleotide binding site was destroyed by mutagenesis of a highly conserved residue predicted to be needed for the binding, does not bind ATP. Another variant, FtsA104, cannot be phosphorylated because the predicted phosphorylatable residue has been replaced by a non-phosphorylatable one. This protein although unable to bind ATP in vitro, is able to rescue the reversible ftsA2, the irreversible ftsA3 and, almost with the same efficiency, the ftsA16 amber alleles. Consequently, phosphorylation and ATP binding may not be essential for the function of FtsA. Alternatively they may have a regulatory role on the action of FtsA in the septator. Previous ArticleNext Article Volume 13Issue 201 October 1994In this issue RelatedDetailsLoading ...