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Effects of Protein Phosphorylation on the Sulphonylurea Receptor of the Pancreatic β-Cell

1997; Springer Nature; Linguagem: Inglês

10.1007/978-1-4899-1819-2_8

2214-8019

Ichiro Niki, Barbara Coles, Frances M. Ashcroft, Stephen J.H. Ashcroft,

Metabolism, Diabetes, and Cancer

There is evidence that protein phosphorylation may modulate the action of diazoxide on the β-cell K-ATP channel [1] and the sulphonylurea receptor [2–3]. In this paper, we have further investigated the role of phosphorylation in the action of diazoxide on the β-cell sulphonylurea receptor by carrying out time-course studies on inhibition of [3H]-gliben-clamide binding by MgATP and diazoxide, and by examining effects of phosphatase inhibitors on binding. We also investigated the presence of protein kinase and protein phosphatase activities in β-cell membrane fractions.