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Vitamin D supplementation for four months causes cardiac hypertrophy and diastolic dysfunction in normal rats

2013; Wiley; Volume: 27; Issue: S1 Linguagem: Inglês

10.1096/fasebj.27.1_supplement.lb281

1530-6860

Dos Santos, Bruna Paola Murino Rafacho, Andréa Gonçalves, Renata Cândido, Vanessa M. Ferreira, Meliza Goi Roscani, Marcos Ferreira Minicucci, Álvaro Oscar Campana, Leonardo A. M. Zornoff, Paula S. Azevedo, Sérgio Alberto Rupp de Paiva,

Cardiovascular Disease and Adiposity

The aim of the study was to evaluate different vitamin D supplementation doses in the cardiac structure and function in normal rats. Methods 46 male rats allocated into 3 groups: control (C, n=14) received standard chow; D3 (n=16) and D10 (n=16) received 3,000 and 10,000 IU of cholecalciferol/kg chow for 4 months, after was performed echocardiography evaluation and morphometry. Results The posterior wall thickness (C=1.3(1.3–1.5); D3=1.8(1.8–2.0); D10=1.8(1.7–1.8) mm; p<0.001), relative wall thickness (C=0.39±0.04; D3=0.52±0.09; D10=0.50±0.06; p<0.001) and left ventricle mass/weight body (WB)(C=1.6±0.2; D3=2.0±0.3; D10=2.0±0.4 mg/g; p<0.001) were higher in supplemented groups compared to C. The supplemented animals presented higher left atrial diameter/aortic diameter (C=1.1±0.1; D3=1.4±0.2; D10=1.3±0.2; p<0,001). The worst diastolic dysfunction was in D10, which led to lower values of tricuspid E/A‐wave ratio (C=0.66(0.57–1.28); D3=0.58(0.52–0.71); D10=0.53(0.47–0.60); p=0.04) and higher values of right ventricle weight/WB(C=0.46±0.03; D3=0.46±0.03; D10=0.50±0.05 mg/g; p=0.014) compared to C. Conclusion Normal rats supplemented with vitamin D showed concentric cardiac hypertrophy and diastolic dysfunction with impaired right side heart in animals supplemented with the highest dose.