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Replacement of histone H1 by H5 in vivo does not change the nucleosome repeat length of chromatin but increases its stability.

1990; Springer Nature; Volume: 9; Issue: 5 Linguagem: Inglês

10.1002/j.1460-2075.1990.tb08285.x

1460-2075

Jie Sun, Ali Z, R. Lurz, A. Ruiz‐Carrillo,

Machine Learning in Bioinformatics

Research Article1 May 1990free access Replacement of histone H1 by H5 in vivo does not change the nucleosome repeat length of chromatin but increases its stability. J.M. Sun J.M. Sun Cancer Research Center, Laval University School of Medicine, L'Hotel-Dieu de Québec, Canada. Search for more papers by this author Z. Ali Z. Ali Cancer Research Center, Laval University School of Medicine, L'Hotel-Dieu de Québec, Canada. Search for more papers by this author R. Lurz R. Lurz Cancer Research Center, Laval University School of Medicine, L'Hotel-Dieu de Québec, Canada. Search for more papers by this author A. Ruiz-Carrillo A. Ruiz-Carrillo Cancer Research Center, Laval University School of Medicine, L'Hotel-Dieu de Québec, Canada. Search for more papers by this author J.M. Sun J.M. Sun Cancer Research Center, Laval University School of Medicine, L'Hotel-Dieu de Québec, Canada. Search for more papers by this author Z. Ali Z. Ali Cancer Research Center, Laval University School of Medicine, L'Hotel-Dieu de Québec, Canada. Search for more papers by this author R. Lurz R. Lurz Cancer Research Center, Laval University School of Medicine, L'Hotel-Dieu de Québec, Canada. Search for more papers by this author A. Ruiz-Carrillo A. Ruiz-Carrillo Cancer Research Center, Laval University School of Medicine, L'Hotel-Dieu de Québec, Canada. Search for more papers by this author Author Information J.M. Sun1, Z. Ali1, R. Lurz1 and A. Ruiz-Carrillo1 1Cancer Research Center, Laval University School of Medicine, L'Hotel-Dieu de Québec, Canada. The EMBO Journal (1990)9:1651-1658https://doi.org/10.1002/j.1460-2075.1990.tb08285.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info In vivo competition between histones H1 and H5 for chromatin has been studied in rat sarcoma XC10 cells transfected with a glucocorticoid responsive MMTV-H5 gene. Activation of H5 expression results in accumulation of H5 in the nuclei where it partially replaces H1. H5 displaces H1 from its primary binding sites presumably during chromatin replication and also binds with high affinity to secondary chromatin sites normally not occupied by H1. Replacement of H1 by H5 to levels similar to those of mature chicken erythrocytes does not alter the nucleosome repeat length of chromatin. This indicates that H5 is not solely responsible for the increase in nucleosome spacing of maturing erythroid cells. Exchange of H1 by H5 in vivo or in vitro results in a higher compaction/stability of chromatin. Previous ArticleNext Article Volume 9Issue 51 May 1990In this issue RelatedDetailsLoading ...