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Integrative transformation of Caenorhabditis elegans

1986; Springer Nature; Volume: 5; Issue: 10 Linguagem: Inglês

10.1002/j.1460-2075.1986.tb04550.x

1460-2075

Andrew Fire,

Evolution and Genetic Dynamics

Article1 October 1986free access Integrative transformation of Caenorhabditis elegans Andrew Fire Andrew Fire Medical Research Council, Laboratory of Molecular Biology, Cambridge CB2 2QH, UK Search for more papers by this author Andrew Fire Andrew Fire Medical Research Council, Laboratory of Molecular Biology, Cambridge CB2 2QH, UK Search for more papers by this author Author Information Andrew Fire1 1Medical Research Council, Laboratory of Molecular Biology, Cambridge CB2 2QH, UK The EMBO Journal (1986)5:2673-2680https://doi.org/10.1002/j.1460-2075.1986.tb04550.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info A technique for introducing exogenous DNA into the chromosomes of the nematode Caenorhabditis elegans is presented. A cloned C. elegans amber suppressor tRNA gene, sup-7, is used as a selectable marker. The activity of this amber suppressor is selected for by injecting worms which carry an amber termination mutation in a gene (tra-3) whose function is required for fertility. Transient expression of sup-7 is evidenced by the presence of fertile (rescued) animals in the generation after injection. In a fraction of cases, these fertile animals give rise to stable suppressor lines (eight have been characterized so far). Each of the stable suppressor lines carries injected DNA sequences. The suppressor activities have been mapped to chromosomal loci, indicating that the exogenous DNA has integrated into the genome. This technique has been used to introduce a chimeric gene containing a Drosophila heat shock promoter element fused to coding sequences from the Escherichia coli β-galactosidase gene. This chimeric gene functions and is heat inducible in the resulting stably transformed lines. Previous ArticleNext Article Volume 5Issue 101 October 1986In this issue RelatedDetailsLoading ...