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Mammalian nuclei contain foci which are highly enriched in components of the pre-mRNA splicing machinery.

1991; Springer Nature; Volume: 10; Issue: 1 Linguagem: Inglês

10.1002/j.1460-2075.1991.tb07936.x

1460-2075

Maria Carmo‐Fonseca, David Tollervey, Rainer Pepperkok, Silvia M.L. Barabino, Andreas Merdes, Claudia Brunner, Phillip D. Zamore, M. R. Green, Eduard C. Hurt, Angus I. Lamond,

RNA modifications and cancer

Research Article1 January 1991free access Mammalian nuclei contain foci which are highly enriched in components of the pre-mRNA splicing machinery. M. Carmo-Fonseca M. Carmo-Fonseca European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author D. Tollervey D. Tollervey European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author R. Pepperkok R. Pepperkok European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author S. M. Barabino S. M. Barabino European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author A. Merdes A. Merdes European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author C. Brunner C. Brunner European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author P. D. Zamore P. D. Zamore European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author M. R. Green M. R. Green European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author E. Hurt E. Hurt European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author A. I. Lamond A. I. Lamond European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author M. Carmo-Fonseca M. Carmo-Fonseca European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author D. Tollervey D. Tollervey European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author R. Pepperkok R. Pepperkok European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author S. M. Barabino S. M. Barabino European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author A. Merdes A. Merdes European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author C. Brunner C. Brunner European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author P. D. Zamore P. D. Zamore European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author M. R. Green M. R. Green European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author E. Hurt E. Hurt European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author A. I. Lamond A. I. Lamond European Molecular Biology Laboratory, Heidelberg, FRG. Search for more papers by this author Author Information M. Carmo-Fonseca1, D. Tollervey1, R. Pepperkok1, S. M. Barabino1, A. Merdes1, C. Brunner1, P. D. Zamore1, M. R. Green1, E. Hurt1 and A. I. Lamond1 1European Molecular Biology Laboratory, Heidelberg, FRG. The EMBO Journal (1991)10:195-206https://doi.org/10.1002/j.1460-2075.1991.tb07936.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The organization of the major snRNP particles in mammalian cell nuclei has been analysed by in situ labelling using snRNA-specific antisense probes made of 2′-OMe RNA. U3 snRNA is exclusively detected in the nucleolus while all the spliceosomal snRNAs are found in the nucleoplasm outside of nucleoli. Surprisingly, U2, U4, U5 and U6 snRNAs are predominantly observed in discrete nucleoplasmic foci. U1 snRNA is also present in foci but in addition is detected widely distributed throughout the nucleoplasm. An anti-peptide antibody specific for the non-snRNP splicing factor U2AF reveals it to have a similar distribution to U1 snRNA. Co-localization studies using confocal fluorescence microscopy prove that U2AF is present in the snRNA-containing foci. Antibody staining also shows the foci to contain snRNP-specific proteins and m3G-cap structures. The presence of major components of the nuclear splicing apparatus in foci suggests that these structures may play a role in pre-mRNA processing. Previous ArticleNext Article Volume 10Issue 11 January 1991In this issue RelatedDetailsLoading ...