The initiation mass for DNA replication in Escherichia coli K-12 is dependent on growth rate.
1994; Springer Nature; Volume: 13; Issue: 9 Linguagem: Inglês
10.1002/j.1460-2075.1994.tb06485.x
ISSN1460-2075
AutoresSture Wold, Kirsten Skarstad, Henning Steen, Trond Stokke, Erik Boye,
Tópico(s)DNA and Nucleic Acid Chemistry
ResumoResearch Article1 May 1994free access The initiation mass for DNA replication in Escherichia coli K-12 is dependent on growth rate. S. Wold S. Wold Department of Biophysics, Institute for Cancer Research, Montebello, Oslo, Norway. Search for more papers by this author K. Skarstad K. Skarstad Department of Biophysics, Institute for Cancer Research, Montebello, Oslo, Norway. Search for more papers by this author H.B. Steen H.B. Steen Department of Biophysics, Institute for Cancer Research, Montebello, Oslo, Norway. Search for more papers by this author T. Stokke T. Stokke Department of Biophysics, Institute for Cancer Research, Montebello, Oslo, Norway. Search for more papers by this author E. Boye E. Boye Department of Biophysics, Institute for Cancer Research, Montebello, Oslo, Norway. Search for more papers by this author S. Wold S. Wold Department of Biophysics, Institute for Cancer Research, Montebello, Oslo, Norway. Search for more papers by this author K. Skarstad K. Skarstad Department of Biophysics, Institute for Cancer Research, Montebello, Oslo, Norway. Search for more papers by this author H.B. Steen H.B. Steen Department of Biophysics, Institute for Cancer Research, Montebello, Oslo, Norway. Search for more papers by this author T. Stokke T. Stokke Department of Biophysics, Institute for Cancer Research, Montebello, Oslo, Norway. Search for more papers by this author E. Boye E. Boye Department of Biophysics, Institute for Cancer Research, Montebello, Oslo, Norway. Search for more papers by this author Author Information S. Wold1, K. Skarstad1, H.B. Steen1, T. Stokke1 and E. Boye1 1Department of Biophysics, Institute for Cancer Research, Montebello, Oslo, Norway. The EMBO Journal (1994)13:2097-2102https://doi.org/10.1002/j.1460-2075.1994.tb06485.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info It is widely accepted that the initiation mass of Escherichia coli is constant and independent of growth rate, and therefore is an important parameter in the regulation of initiation of DNA replication. We have used flow cytometry to measure the initiation mass of E. coli K-12 cells as a function of growth rate. The average initiation mass was determined by two methods: (i) from a mathematical relationship between average cell mass, cell age at initiation and number of origins present in the cells, and (ii) directly from the cell mass distribution. The light scattering signal from individual cells and the protein content per cell were employed as measures of cell mass. The initiation mass was found to increase monotonically with decreasing growth rate, being 1.6 times higher (light scattering) or 2.1 times higher (protein content) at 0.3 than at 2.5 doublings per hour. We conclude that the initiation mass is dependent on growth rate. This finding indicates that the control for timing of initiation is not governed by a direct connection between mass accumulation and the molecule(s) determining initiation of replication. Previous ArticleNext Article Volume 13Issue 91 May 1994In this issue RelatedDetailsLoading ...
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