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Divergent molecular phenotypes of KG1 and KG1a myeloid cell lines

1986; Elsevier BV; Volume: 68; Issue: 5 Linguagem: Inglês

10.1182/blood.v68.5.1101.1101

1528-0020

Andrew J. Furley, BR Reeves, Shuki Mizutani, LJ Altass, SM Watt, MC Jacob, Peter J. van den Elsen, Cox Terhorst, MF Greaves,

T-cell and B-cell Immunology

Abstract The cell line KG1 derived from a patient with erythroleukemia in myeloblastic relapse has the composite phenotype and functional repertoire of myeloblasts. In marked contrast, its subline KG1a has lost myeloid features, acquired new karyotypic markers, and has three characteristics associated with immature T cells: low-level expression of the T cell receptor beta mRNA (but not alpha) transcribed from a germline gene; high-level expression of T3 delta mRNA and intracellular, but not cell surface, T3 protein; and expression of the CD7/gp40 T cell-associated membrane antigen. Both KG1 and KG1a transcribe unrearranged IgH genes. These data suggest that either the KG1 cell line was derived from a common myeloid-lymphoid progenitor or that the KG1a subline phenotype is aberrant.