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Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay

2009; Springer Science+Business Media; Linguagem: Inglês

10.1007/978-1-60327-164-6_7

1940-6029

Andrew M. Kropinski, Amanda Mazzocco, Thomas K. Waddell, Erika J. Lingohr, Roger P. Johnson,

Monoclonal and Polyclonal Antibodies Research

The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described.