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Chapter 23 High-Gradient Magnetic Cell Sorting

1994; Elsevier BV; Linguagem: Inglês

10.1016/s0091-679x(08)61086-9

0091-679X

Andreas Radbruch, Birgit Mechtold, Andreas Thiel, Stefan Miltenyi, E. Pflüger,

Microfluidic and Bio-sensing Technologies

This chapter describes magnetic cell separation based on staining with colloidal superparamagnetic particles and separation of stained cells in high-gradient magnetic fields. Magnetically labeled cells can be stained simultaneously with fluorochromated antibodies to control and evaluate the quality of magnetic separation or to further analyze and sort the cells by flow cytometry or microscopy. Although magnetism provides only one parameter for separation, magnetic cell sorting is superior to fluorescence-activated cell sorting (FACS) in several aspects and, in others, a valuable complementation of it. In FACS, the most powerful multiparameter sorting technology, cells are analyzed one by one and sorted one after the other. Due to its origin in immunology, high-gradient magnetic cell sorting has been used primarily for the isolation of various cell types from human and animal hematopoietic systems. It has been used also for separation of plant cells and bacteria.