4 Transformation of Bacteria by Plasmid DNA
1984; Academic Press; Linguagem: Inglês
10.1016/s0580-9517(09)70052-2
ISSN0580-9517
AutoresJ. R. Saunders, Andrew Docherty, G. O. Humphreys,
Tópico(s)Bacteriophages and microbial interactions
ResumoBacterial transformation systems can be divided into two categories: natural (physiological) and artificial. In naturally transformable species, the cells become competent (able to take up DNA into a deoxyribonuclease-resistant form) at particular stages in the growth cycle or after shifts in nutritional status of cultures. In others, competence depends on the presence of components of the cell envelope such as membrane proteins, autolysins, or pili. However, many bacterial species do not become naturally competent under normal culture conditions and must be rendered permeable to DNA artificially. This may involve treating whole cells with high concentrations of divalent cations. This chapter describes the techniques currently available for transforming bacteria with plasmid DNA. Methods for introducing purified plasmid DNA into intact bacteria, bacterial protoplasts, and spheroplasts are discussed.
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