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Evaluation of oxidative stress and energy metabolism in the heart of rats supplemented with different vitamin D doses

2012; Wiley; Volume: 26; Issue: S1 Linguagem: Inglês

10.1096/fasebj.26.1_supplement.385.8

1530-6860

Dos Santos, Bruna Paola Murino Rafacho, Lidiane P. Ardisson, Andréa Gonçalves, Vanessa Pires, Marcos Ferreira Minicucci, Paula S. Azevedo, Álvaro Oscar Campana, Ana Angélica Henrique Fernandes, Leonardo A. M. Zornoff, Sérgio Alberto Rupp de Paiva,

Exercise and Physiological Responses

The aim was to evaluate of different vitamin D supplementation doses in the heart of normal rats. Methods 86 male rats allocated into 4 groups: control (C, n=21) received standard chow; D3 (n=22), D5 (n=22) and D10 (n=21) received 3000, 5000 and 10000 IU of cholecalciferol/kg chow for 2 months Results Lipid hydroperoxide was higher in D10 compared to other groups (C=143.8±13.9; D3=143.8±13.9; D5=176.3±16.1; D10=179.6±11.8 nmol/g tissue; p<0.001); the activity of antioxidants enzymes: superoxide dismutase was lower in the supplemented groups (C=21.6±4.1; D3=12.9±1.2; D5=13.2±1.7; D10=13.1±1.4 nmol/g protein; p<0,001) and glutathione peroxidase was lower in D3 and D10 compared to the C (C=40.4±6.2; D3=31.5±4.6; D5=35.5±5.2; D10=29.7±3.1 ìmol/g tissue; p<0,001). Activity of the enzymes: â‐hydroxyacyl coenzyme‐A dehydrogenase was lower in D5 and D10 compared to C and D3(C=69.9±3.8; D3=65.8±4.6; D5=44.7±3.3; D10=34.4±1.8 nmol/mg; p<0.001); citrate synthase was lower in D10 compared to other groups (C=39.8±3.2; D3=40.4±2.8; D5=39.7±2.7; D10=34.5±4.0; umol/g tissue; p=0.003) and lactate dehydrogenase (C=220±6.4; D3=209±3.6; D%=199±4.1; D10=256±3.4 nmol/mg; p<0.001) was higher in D10 compared to other groups. Vitamin D treatment showed dose‐dependent effect. Conclusion Normal rats supplemented with vitamin D3 presented higher oxidative stress and changes of cardiac energy metabolism.