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Bladder Surface Glycosaminoglycans: An Epithelial Permeability Barrier

1990; Lippincott Williams & Wilkins; Volume: 143; Issue: 1 Linguagem: Inglês

10.1016/s0022-5347(17)39897-x

1527-3792

Lowell Parsons, Don Boychuk, Susan Jones, Robert E. Hurst, Hugh J. Callahan,

Seaweed-derived Bioactive Compounds

No AccessJournal of Urology1 Jan 1990Bladder Surface Glycosaminoglycans: An Epithelial Permeability Barrier L. Parsons, D. Boychuk, S. Jones, R. Hurst, and H. Callahan L. ParsonsL. Parsons , D. BoychukD. Boychuk , S. JonesS. Jones , R. HurstR. Hurst , and H. CallahanH. Callahan View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)39897-XAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Sulfated polysaccharide's ability to modulate the movement of small molecules was examined both in vivo and in vitro. For the in vivo test, the rabbit bladder was utilized and C-14 labeled urea 45-Ca, or 3H2O was placed into the lumen of control bladders, bladders pretreated with protamine sulfate (20mg./cc) and bladders pretreated with protamine sulfate (20mg./cc) plus pentosanpolysulfate (PPS), 10mg./cc. After 45 minutes, the controls absorbed 21% of the urea, 16% of the calcium, and 38% of the 3H2O; the protamine treated group 40% urea, 23% calcium, and 51% H2O; the PPS only group 22% urea and the protamine plus PPS group absorbed 24% of urea, 18% calcium, and 44% water. Differences between the control and protamine groups were statistically significant, p <0.01 for urea 45-Ca and 3H2O. The bladder mucosa contained a significantly higher concentration of urea and calcium after protamine treatment which were both reversed by PPS (p <0.01) while 3H2O content went down significantly (p = 0.03), reflecting a loss of the hydrophilic effect of bladder GAG. The control mucosas had 250cpm/mg. tissue urea for Ca 64cpm/mg. and water 262cpm/mg., the protamine group urea 498cpm/mg., Ca 190cpm/mg., and H2O 139cpm/mg.; the protamine plus PPS group urea 344cpm/mg., Ca 129cpm/mg., and water 168cpm/mg. For the in vitro studies, an Ussing chamber was employed. Normal rabbit bladder membranes were placed in the chambers and the potential difference was zeroed across the membrane. There were three groups, membranes that were treated only with the irrigating solution, membranes pretreated with protamine, and membranes pretreated with protamine plus PPS. At the end of 40 minutes, there was an approximately 1.2% movement of urea across the control membrane, a 3.5% movement across the protamine treated membrane (a significant increase p <0.001) and a 1.1% movement across the protamine plus PPS treated membrane. It would appear that the surface polysaccharide may play an important role as a bladder permeability barrier in modulating both charged and uncharged small molecule movement in that its ability to impair such movement can be inhibited by protamine and this protamine effect can be reversed by a treatment with an exogenous sulfated polysaccharide. © 1990 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited byWilliams N, Barnard L, Allender C, Bowen J, Gumbleton M, Harrah T, Raja A and Joshi H (2015) Evidence of Nonuniformity in Urothelium Barrier Function between the Upper Urinary Tract and BladderJournal of Urology, VOL. 195, NO. 3, (763-770), Online publication date: 1-Mar-2016.Janssen D, van Wijk X, Jansen K, van Kuppevelt T, Heesakkers J and Schalken J (2012) The Distribution and Function of Chondroitin Sulfate and Other Sulfated Glycosaminoglycans in the Human Bladder and Their Contribution to the Protective Bladder BarrierJournal 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Volume 143Issue 1January 1990Page: 139-142 Advertisement Copyright & Permissions© 1990 by The American Urological Association Education and Research, Inc.MetricsAuthor Information L. Parsons More articles by this author D. Boychuk More articles by this author S. Jones More articles by this author R. Hurst More articles by this author H. Callahan More articles by this author Expand All Advertisement PDF downloadLoading ...