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EFFICACY ASSESSMENT OF BACTERICIDE CONTAINING DIDECYLDIMETHYLAMMONIUM CHLORIDE ON BACTERIA FOUND IN SOAK LIQUOR AT DIFFERENT EXPOSURE TIMES

2010; American Leather Chemists Association; Volume: 105; Issue: 11 Linguagem: Inglês

0002-9726

Didem Berber, Meral Birbir, Hüsniye Hacıoğlu,

Fermentation and Sensory Analysis

In this study, the efficacy of the bactericide containing didecyldimethylammonium chloride (quaternary ammonium compound) on the bacteria found in the main soak liquor at tannery was examined at different exposure times of 10 min., 30 min., 60 min., 120 min., 180 min., 240 min., 300 min., 360 min., 420 min. and 480 min. The presence of proteolytic and lipolytic mesophilic bacterial populations in high numbers in the main soak liquors showed that the recommended concentration of the bactericide (0.4 g/l) was not effective to control bacterial populations. Based on these results, the recommended concentration of the bactericide in the main soaking process was doubled (0.8 g/l) and it was observed that this concentration was considerably effective in controlling the bacterial growth. In addition, the bacterial flora in the main soak liquor treated with this bactericide was examined. Enterobacter gergoviae, Enterobacter sakazakii, Enterobacter amnigenus biogrup I, Enterobacter cloaceae, Pseudomonas fluorescens, Pseudomonas luteola, Pseudomonas aeruginosa, Pseudomonas putida, Enterococcus avium, Enterococcus faecium, Lactococcus lactis ssp. lactis, Aerococcus viridans, Vibrio parahaemolyticus, Kocuria varians, Staphylococcus hominis, Staphylococcus capitis, Staphylococcus sciuri, Staphylococcus xylosus, Staphylococcus cohnii ssp. urealyticus, Staphylococcus epidermidis, Staphylococcus intermedius, Staphylococcus haemolyticus, Staphylococcus lugdunensis, Staphylococcus cohnii ssp. cohnii, Staphylococcus warneri and Micrococcus spp. were isolated and identified from the main soak liquor after doubling the bactericide concentration. As a conclusion, the recommended dose of commonly used bactericides should be tested periodically in main soaking process at tanneries to inactivate various bacterial populations found in soak liquors. RESUMENEn este estudio, la eficacia de los bactericidas que contienen cloruro de didecyldimetilamonio (compuesto de amonio cuaternario) sobre la bacteria que se encuentra en el licor del remojo principal en la curtiembre fue examinado a diferentes tiempos de exposicion de 10min, 30min, 60min, 120min, 180 min., 240 min., 300 min., 360 min., 420 min. y 480 min. La presencia de poblaciones de bacterias proteoliticas y lipoliticas mesofilas en gran numero en el licor del remojo principal mostraron que la concentracion recomendada del bactericida (0,4 g / l) no fue efectiva para controlar las poblaciones bacterianas. Con base en estos resultados, la concentracion recomendada del bactericida en el proceso de remojo principal se duplico (0,8 g / l) y se observo que esta concentracion era considerablemente efectiva para controlar el crecimiento bacteriano. Ademas, la flora bacteriana en el licor del remojo principal tratados con este bactericida fue examinado. Enterobacter gergoviae, Enterobacter sakazakii, Enterobacter amnigenus biogrup I, Enterobacter cloaceae, Pseudomonas fluorescens, Pseudomonas luteola, Pseudomonas aeruginosa, Pseudomonas putida, Enterococcus avium, Enterococcus faecium, Lactococcus lactis ssp. lactis, Aerococcus viridans, Vibrio parahaemolyticus, Kocuria varians, Staphylococcus hominis, Staphylococcus capitis, Staphylococcus sciuri, Staphylococcus xylosus, Staphylococcus cohnii ssp. urealyticus, Staphylococcus epidermidis, Staphylococcus intermedius, Staphylococcus haemolyticus, Staphylococcus lugdunensis, Staphylococcus cohnii ssp. cohnii, Staphylococcus warneri and Micrococcus spp. fueron aislados e identificados a partir del licor del remojo principal despues de doblar la concentracion del bactericida. Como conclusion, la dosis recomendada de bactericidas de uso general debe ser probado periodicamente en el proceso de remojo principal en las curtiembres para inactivar diversas poblaciones de bacterias en el licor de remojo.