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Effect of electro-acupuncture at Foot-Yangming Meridian on somatostatin and expression of somatostatin receptor genes in rabbits with gastric ulcer

2006; Baishideng Publishing Group; Volume: 12; Issue: 11 Linguagem: Inglês

10.3748/wjg.v12.i11.1761

2219-2840

Shou-Xiang Yi,

Biochemical effects in animals

AIM:To discuss the protective effect of electroacupuncture at the Foot-Yangming Meridian on gastric mucosal lesion, somatostatin (SS) and the expression of SS receptor genes (SSR1 mRNA ) in rabbits with gastric ulcer and to further explore the relative specificity of meridians and viscera at gene expression level. METHODS:Forty rabbits were randomly divided into control group (A), gastric ulcer model group (B), Foot-Yangming Meridian group (C), Foot-Shaoyang Meridian group (D) and Foot-Taiyang Meridian group (E).The gastric ulcer model was prepared by infusing alcohol into stomach.Groups C - E were treated with electroacupuncture at points along the above meridians using meridian stimulating instruments for 7 days respectively.By the end of treatment, the index of gastric ulcer was determined, the amount of epidermal growth factor(EGF) and somatostatin was measured by radioimmunoassay (RIA).SS-R1 mRNA expression in gastric mucosa was determined by RT-PCR. RESULTS:The value of EGF in model group was obviously lower(73.6± 14.8 vs 91.3 ± 14.9 pg/mL, P < 0.01) than that in control group.The index of gastric ulcer, content of SS and expression of SSR1 mRNA in gastric mucosa were significantly higher than those in control group(24.88± 6.29 vs 8.50 ± 2.98 scores, P < 0.01; 2978.6 ± 587.6 vs 1852.4 ± 361.7 mIU/mL, P < 0.01; 2.56 ± 0.25 vs 1.04 ± 0.36, P <0.01).The value of EGF in Foot-Yangming Meridian group was higher than that in model group(92.2± 6.7 vs 73.6 ± 14.8 pg/mL, P < 0.01).The index of gastric ulcer, content of SS and expression of SS-R1 mRNA in gastric mucosa were significantly lower than those in control group(10.88± 3.23 vs 24.88 ± 6.29 scores, P < 0.01; 1800.2 ± 488 vs 2978.6 ± 587.6 mIU/mL, P < 0.01; 1.07 ± 0.08 vs 2.56 ± 0.25mIU/mL, P < 0.01).Compared to the model group, the content of SS and expression of SSR1 mRNA in gastric mucosa in Foot-Shaoyang Meridian group decreased (2441.0 ± 488.vs 2978.6 ± 587.6 mIU/mL, P < 0.05;1.73± 0.16 vs 2.56 ± 0.25 mIU/mL, P < 0.01).But the above parameters in Foot-Taiyang Meridian group did not improve and were significantly different from those in Foot-Yangming Meridian group (P < 0.05). CONCLUSION:Electro-acupuncture at Foot-Yangming Meridian can protect gastric mocusa against injury.The mechanism may be releted to the regulation of brain-gut peptides and the expression of SSR1 mRNA.