Portal de Periódicos da CAPES

Three-dimensional structure of the regular surface glycoprotein layer of Halobacterium volcanii from the Dead Sea

1988; Springer Nature; Volume: 7; Issue: 5 Linguagem: Inglês

10.1002/j.1460-2075.1988.tb02974.x

1460-2075

Martin Kessel, Ivo Wildhaber, Simone Cohen, Wolfgang Baumeister,

Bacteriophages and microbial interactions

Article1 May 1988free access Three-dimensional structure of the regular surface glycoprotein layer of Halobacterium volcanii from the Dead Sea Martin Kessel Martin Kessel Department of Membrane and Ultrastructure Research, Hadassah Medical School, The Hebrew University, PO Box 1172, Jerusalem 91-010, Israel Search for more papers by this author Ivo Wildhaber Ivo Wildhaber Max-Planck Institut für Biochemie, D-8033 Martinsried, FRG Search for more papers by this author Simone Cohen Simone Cohen Department of Membrane and Ultrastructure Research, Hadassah Medical School, The Hebrew University, PO Box 1172, Jerusalem 91-010, Israel Search for more papers by this author Wolfgang Baumeister Wolfgang Baumeister Max-Planck Institut für Biochemie, D-8033 Martinsried, FRG Search for more papers by this author Martin Kessel Martin Kessel Department of Membrane and Ultrastructure Research, Hadassah Medical School, The Hebrew University, PO Box 1172, Jerusalem 91-010, Israel Search for more papers by this author Ivo Wildhaber Ivo Wildhaber Max-Planck Institut für Biochemie, D-8033 Martinsried, FRG Search for more papers by this author Simone Cohen Simone Cohen Department of Membrane and Ultrastructure Research, Hadassah Medical School, The Hebrew University, PO Box 1172, Jerusalem 91-010, Israel Search for more papers by this author Wolfgang Baumeister Wolfgang Baumeister Max-Planck Institut für Biochemie, D-8033 Martinsried, FRG Search for more papers by this author Author Information Martin Kessel1, Ivo Wildhaber2, Simone Cohen1 and Wolfgang Baumeister2 1Department of Membrane and Ultrastructure Research, Hadassah Medical School, The Hebrew University, PO Box 1172, Jerusalem 91-010, Israel 2Max-Planck Institut für Biochemie, D-8033 Martinsried, FRG The EMBO Journal (1988)7:1549-1554https://doi.org/10.1002/j.1460-2075.1988.tb02974.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info A three-dimensional reconstruction from electron micrographs of negatively stained cell envelopes of Halobacterium volcanii has revealed the structure of the surface glycoprotein to a resolution of 2 nm. The glycoprotein is arranged on a p6 lattice with a lattice constant of 16.8 nm. It forms 4.5 nm high, dome-shaped, morphological complexes with a narrow pore at the apex opening into a 'funnel' towards the cell membrane. The polarity of the structure was derived from freeze-etching experiments and 'edge' views. Six radial protrusions emanate from each morphological complex and join around the 3-fold axis to provide lateral connectivity. Using the primary structure of the surface glycoprotein of the closely related species Halobacterium halobium (Lechner and Sumper, 1987) and the cell envelope profile from a previous X-ray analysis of the same species (Blaurock et al., 1976) we have integrated our reconstruction into a model of halobacterial cell envelope. Previous ArticleNext Article Volume 7Issue 51 May 1988In this issue RelatedDetailsLoading ...