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Influence of the codon following the AUG initiation codon on the expression of a modified lacZ gene in Escherichia coli.

1987; Springer Nature; Volume: 6; Issue: 8 Linguagem: Inglês

10.1002/j.1460-2075.1987.tb02530.x

1460-2075

A. C. Looman, J. Bodlaender, LISA J. COMSTOCK, Diane Eaton, Parkash Jhurani, Harry Boer, P.H. Van Knippenberg,

Genomics and Phylogenetic Studies

Research Article1 August 1987free access Influence of the codon following the AUG initiation codon on the expression of a modified lacZ gene in Escherichia coli. A. C. Looman A. C. Looman Department of Biochemistry, University of Leiden, The Netherlands. Search for more papers by this author J. Bodlaender J. Bodlaender Department of Biochemistry, University of Leiden, The Netherlands. Search for more papers by this author L. J. Comstock L. J. Comstock Department of Biochemistry, University of Leiden, The Netherlands. Search for more papers by this author D. Eaton D. Eaton Department of Biochemistry, University of Leiden, The Netherlands. Search for more papers by this author P. Jhurani P. Jhurani Department of Biochemistry, University of Leiden, The Netherlands. Search for more papers by this author H. A. de Boer H. A. de Boer Department of Biochemistry, University of Leiden, The Netherlands. Search for more papers by this author P. H. van Knippenberg P. H. van Knippenberg Department of Biochemistry, University of Leiden, The Netherlands. Search for more papers by this author A. C. Looman A. C. Looman Department of Biochemistry, University of Leiden, The Netherlands. Search for more papers by this author J. Bodlaender J. Bodlaender Department of Biochemistry, University of Leiden, The Netherlands. Search for more papers by this author L. J. Comstock L. J. Comstock Department of Biochemistry, University of Leiden, The Netherlands. Search for more papers by this author D. Eaton D. Eaton Department of Biochemistry, University of Leiden, The Netherlands. Search for more papers by this author P. Jhurani P. Jhurani Department of Biochemistry, University of Leiden, The Netherlands. Search for more papers by this author H. A. de Boer H. A. de Boer Department of Biochemistry, University of Leiden, The Netherlands. Search for more papers by this author P. H. van Knippenberg P. H. van Knippenberg Department of Biochemistry, University of Leiden, The Netherlands. Search for more papers by this author Author Information A. C. Looman1, J. Bodlaender1, L. J. Comstock1, D. Eaton1, P. Jhurani1, H. A. Boer1 and P. H. Knippenberg1 1Department of Biochemistry, University of Leiden, The Netherlands. The EMBO Journal (1987)6:2489-2492https://doi.org/10.1002/j.1460-2075.1987.tb02530.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info In a lacZ expression vector (pMC1403Plac), all 64 codons were introduced immediately 3′ from the AUG initiation codon. The expression of the second codon variants was measured by immunoprecipitation of the plasmid-coded fusion proteins. A 15-fold difference in expression was found among the codon variants. No distinct correlation could be made with the level of tRNA corresponding to the codons and large differences were observed between synonymous codons that use the same tRNA. Therefore the effect of the second codon is likely to be due to the influence of its composing nucleotides, presumably on the structure of the ribosomal binding site. An analysis of the known sequences of a large number of Escherichia coli genes shows that the use of codons in the second position deviates strongly from the overall codon usage in E. coli. It is proposed that codon selection at the second position is not based on requirements of the gene product (a protein) but is determined by factors governing gene regulation at the initiation step of translation. Previous ArticleNext Article Volume 6Issue 81 August 1987In this issue RelatedDetailsLoading ...