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Mini-F encoded proteins: identification of a new 10.5 kilodalton species.

1983; Springer Nature; Volume: 2; Issue: 11 Linguagem: Inglês

10.1002/j.1460-2075.1983.tb01671.x

1460-2075

Françoise Bex, Hakim Karoui, Luis A. Rokeach, Pierre Luc Dreze, Llorenç Valverde, Martine Couturier,

RNA modifications and cancer

Research Article1 November 1983free access Mini-F encoded proteins: identification of a new 10.5 kilodalton species. F. Bex F. Bex Search for more papers by this author H. Karoui H. Karoui Search for more papers by this author L. Rokeach L. Rokeach Search for more papers by this author P. Drèze P. Drèze Search for more papers by this author L. Garcia L. Garcia Search for more papers by this author M. Couturier M. Couturier Search for more papers by this author F. Bex F. Bex Search for more papers by this author H. Karoui H. Karoui Search for more papers by this author L. Rokeach L. Rokeach Search for more papers by this author P. Drèze P. Drèze Search for more papers by this author L. Garcia L. Garcia Search for more papers by this author M. Couturier M. Couturier Search for more papers by this author Author Information F. Bex, H. Karoui, L. Rokeach, P. Drèze, L. Garcia and M. Couturier The EMBO Journal (1983)2:1853-1861https://doi.org/10.1002/j.1460-2075.1983.tb01671.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The elements which ensure the maintenance of the F plasmid are located in its f5 EcoRI restriction fragment. This f5 fragment constitutes a mini-F plasmid showing the same stability and copy number control as the entire F plasmid. The proteins expressed in minicells by wild-type or mutated f5 fragments were analysed by pH gradient two-dimensional electrophoresis. We identified seven f5-encoded polypeptides and located their genes on the F map. Among them, H1, an acidic polypeptide of mol. wt. 10.5 K, had not been detected before. It is in fact the most abundant f5-encoded polypeptide identified so far. In addition, we showed that both 10.5-K and 12-K protein bands detected by SDS-polyacrylamide gel electrophoresis are, respectively, composed of two polypeptides, H1 and H2, G1 and G2, of different isoelectric points. Polypeptides H2 and G2, respectively, share common coding sequences with polypeptides H1 and G1. Their possible biological significance is discussed. The sequences coding for polypeptides H1/H2 and G1/G2 are clustered in a 800-bp long region located between the two mini-F origin sites and are proposed to be organized as an operon. The results reported in the accompanying paper point out the importance of polypeptides G1/G2 and H1/H2 in the relationship between the F plasmid and its host. Previous ArticleNext Article Volume 2Issue 111 November 1983In this issue RelatedDetailsLoading ...