Micromethods for the Characterization of Lipid A-Core and O-Antigen Lipopolysaccharide
2006; Humana Press; Linguagem: Inglês
10.1385/1-59745-167-3
AutoresCristina L. Marolda, Piya Lahiry, Enrique D. Vinés, Soledad Saldı́as, Miguel A. Valvano,
Tópico(s)Salmonella and Campylobacter epidemiology
ResumoMethods for rapid and simple analysis of lipopolysaccharide (LPS) from bacterial whole-cell lysates or membrane preparations have contributed to advancing our knowledge of the genetics of the LPS biogenesis. LPS, a major constituent of the outer membranes in Gram-negative bacteria, has a complex mechanism of synthesis and assembly that requires the coordinated participation of many genes and gene products. This chapter describes a collection of methods routinely used in our laboratory for the characterization of LPS in Escherichia coli and other bacteria.
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