Effective protection of monkeys against death from street virus by post-exposure administration of tissue-culture rabies vaccine.
1971; National Institutes of Health; Volume: 45; Issue: 1 Linguagem: Inglês
Autores
R. Keith Sikes, William F. Cleary, Hilary Koprowski, T. J. Wiktor, Martin M. Kaplan,
Tópico(s)Virology and Viral Diseases
ResumoThree series of experiments on rabies vaccines were carried out on rhesus monkeys using suckling-mouse-brain vaccine, rabbit-brain vaccine, duck-embryo vaccine, and purified, concentrated tissue-culture vaccine. The latter was prepared in a human diploid cell strain and inactivated with beta-propiolactone, and consisted of tissue-culture fluid concentrated 200-fold with a final infectivity titre of 10(9.8) plaque-forming units per ml before inactivation. In the first two series of experiments, several vaccines were tested for relative immunogenicity on a pre-exposure basis. In the third series, a successful model was developed in which a single inoculation of the tissue-culture vaccine administered after exposure to rabies virus, with or without accompanying standard doses of antirabies serum, was evaluated as a method of prevention. A single dose of the tissue-culture vaccine protected 7 out of 8 monkeys from death by street virus. Homologous or heterologous antirabies serum alone gave poor results. The results indicate great promise for prophylaxis in man with one dose, or perhaps a few doses, of highly concentrated, purified tissue-culture vaccine.
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