The effect of ribonuclease on polysomes and ribosomes of bacteria and animal cells
1968; Portland Press; Volume: 107; Issue: 4 Linguagem: Inglês
10.1042/bj1070481
ISSN0306-3283
Autores Tópico(s)Bacteriophages and microbial interactions
ResumoThe mildest treatment with ribonuclease that causes any disaggregation of the polysomes of Escherichia coli or HeLa cells simultaneously attacks the RNA of the constituent ribosomes. It is concluded that the susceptibility to ribonuclease of polysomes does not suggest that they are held together by a strand of messenger RNA. The RNA of the larger sub-unit of bacterial ribosomes has particularly sensitive regions resulting in a non-random degradation. The RNA of the smaller sub-unit of E. coli ribosomes is relatively resistant to ribonuclease attack. The same may be true of the respective sub-units of the intact HeLa-cell ribosome, but both sub-units become very sensitive to ribonuclease on dissociation from each other.
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