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Biotransformation of benzo(a)pyrene in bench scale reactor using laccase of Pycnoporus cinnabarinus

1998; Springer Science+Business Media; Volume: 20; Issue: 12 Linguagem: Inglês

10.1023/a

1573-6776

Rachel Rama, Christian Mougin, François‐Didier Boyer, Albert Kollmann, Christian Malosse, Jean‐Claude Sigoillot,

Biochemical and biochemical processes

The biotransformation of benzo[a]pyrene by purified extracellular laccase of Pycnoporus cinnabarinus was investigated in bench scale reactors. The reaction required the presence of exogenous mediator ABTS. Most of 95% of the substrate was converted within 24 hours. The enzyme preparation oxidised the substrate mainly to benzo[a]pyrene 1,6- 3,6- and 6,12-quinones in a 2/1/1 ratio after 24 h incubation.