THE HYDROLYSIS OF GLYCOGEN BY GLYCEROL EXTRACT OF MUSCLE
1935; Elsevier BV; Volume: 108; Issue: 2 Linguagem: Inglês
10.1016/s0021-9258(18)75291-5
ISSN1083-351X
AutoresAlbert Carruthers, Wei Yung Lee,
Tópico(s)Glycogen Storage Diseases and Myoclonus
ResumoBarbour (1) has claimed that muscle amylase acting on glycogen gives a trisaccharide as the sale product of hydrolysis.This finding is strikingly different from those which have been obtained with pancreatic, salivary, and malt amylases which, it is generally agreed, produce maltose and, when conversion to disaccharide is incomplete, also dextrin (2).Most investigations, it is true, have been carried out with starch as substrate but the studies of Pringsheim (3) and Kuhn (4) lead one to suppose that there is no significant difference between amylopectin, a constituent of starch, and glycogen.Sodium fluoride extracts of muscle acting on glycogen were found by Osborne and Zobel (5) to produce dextrins and maltose and probably some glucose.Among the products of glycogen hydrolysis by potassium chloride extracts of frog muscle Lohmann (6) found glucose and a sugar identified as an amylotriose.If glycerol extracts of muscle split glycogen entirely and solely to a trisaccharide, these extracts must have peculiar properties distinguishing them from other amylase systems.Case (7), without giving any experimental data, stated that he was able to confirm Barbour's results.Experimental work reported here is not in agreement with Barbour's conclusions.EXPERIMENTAL Esperiment I-30 cc. of glycerol extract of rabbit muscle, prepared by the method Barbour employed (1)) were mixed with 10 cc. of phosphate buffer, pH 6.4, and 8.0 gm. of glycogen dissolved in 160 cc. of water.Toluene was added and the mixture was incubated 46 hours.Protein was then removed by heat coagulation.
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