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DNA replication origin and transcriptional enhancer in c-myc gene share the c-myc protein binding sequences.

1989; Springer Nature; Volume: 8; Issue: 13 Linguagem: Inglês

10.1002/j.1460-2075.1989.tb08613.x

1460-2075

H Ariga, Yasuhiro Imamura, Sanae M.M. Iguchi-Ariga,

Genomics and Chromatin Dynamics

Research Article20 December 1989free access DNA replication origin and transcriptional enhancer in c-myc gene share the c-myc protein binding sequences. H. Ariga H. Ariga Faculty of Pharmaceutical Science, Hokkaido University, Sapporo, Japan. Search for more papers by this author Y. Imamura Y. Imamura Faculty of Pharmaceutical Science, Hokkaido University, Sapporo, Japan. Search for more papers by this author S.M. Iguchi-Ariga S.M. Iguchi-Ariga Faculty of Pharmaceutical Science, Hokkaido University, Sapporo, Japan. Search for more papers by this author H. Ariga H. Ariga Faculty of Pharmaceutical Science, Hokkaido University, Sapporo, Japan. Search for more papers by this author Y. Imamura Y. Imamura Faculty of Pharmaceutical Science, Hokkaido University, Sapporo, Japan. Search for more papers by this author S.M. Iguchi-Ariga S.M. Iguchi-Ariga Faculty of Pharmaceutical Science, Hokkaido University, Sapporo, Japan. Search for more papers by this author Author Information H. Ariga1, Y. Imamura1 and S.M. Iguchi-Ariga1 1Faculty of Pharmaceutical Science, Hokkaido University, Sapporo, Japan. The EMBO Journal (1989)8:4273-4279https://doi.org/10.1002/j.1460-2075.1989.tb08613.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info We have previously reported that c-myc protein, or protein(s) complexed with c-myc protein, binds to the region upstream of the first exon of the c-myc gene and that this region contains an origin of cellular DNA replication (ori) and also a transcriptional enhancer. Here we show by Southwestern blotting that c-myc protein binds directly to a 7 bp sequence within the above region. Furthermore, we show that the c-myc protein binding sequences are indispensable for both ori and enhancer functions, but that additional sequences are required for maximal ori and enhancer activities. Thus, c-myc protein is a sequence specific factor which is apparently used both in initiation of DNA replication and in regulation of RNA transcription. Previous ArticleNext Article Volume 8Issue 131 December 1989In this issue RelatedDetailsLoading ...