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[106] Fatty acid composition and pairing in phospholipids of rod outer segments

1982; Academic Press; Linguagem: Inglês

10.1016/s0076-6879(82)81108-7

1557-7988

George P. Miljanich, Edward A. Dratz,

Lipid metabolism and biosynthesis

This chapter discusses the fatty acid composition and pairing in phospholipids of rod outer segments (ROS). In common with the phospholipids of most other biological membranes, the phospholipids of the ROS disk membrane possess a large measure of molecular heterogeneity. The major phospholipids of the ROS disk membrane have now been subfractionated by a simple thin-layer chromatography (TLC) method based on fatty acid composition. An analysis of this subfractionation has yielded estimates of the percentages of each major phospholipid class that are present as the general subclasses containing two saturated fatty acids, one saturated and one unsaturated fatty acid, or two unsaturated fatty acids. The TLC method described in this chapter is a simple procedure that minimizes manipulations of the ROS lipids. This is an important consideration, as minimal handling of these highly polyunsaturated molecules reduces the chances for oxidative degradation.