THE HYDROLYSIS OF GLYCOGEN BY MUSCLE AND LIVER EXTRACTS
1935; Elsevier BV; Volume: 108; Issue: 2 Linguagem: Inglês
10.1016/s0021-9258(18)75292-7
ISSN1083-351X
Autores Tópico(s)Metabolism and Genetic Disorders
ResumoA study of the chemical properties of the products of glycogen hydrolysis and their derivatives led to the view previously reported (1) that glycerol extract of rabbit muscle hydrolyzes glycogen to maltose.It appeared that the products examined also contained glucose and some higher polysaccharide, probably dextrin.Experiments reported here illustrate more thoroughly the enzymic hydrolysis of glycogen. MethodsGlycogen Preparation and Determination-Glycogen was prepared from oyster or liver by repeated digestion in KOH solution and precipitation by alcohol.The determination of glycogen during the course of digestion was performed by transferring a sample (usually 5 cc.) of the digestion mixture to a centrifuge tube (capacity about 17 cc.)containing about 0.5 gm. of solid KOH.The tube was immediately immersed in a bath of boiling water and heated, with occasional stirring, for 20 minutes.The tube and contents were cooled and a volume of absolute alcohol, equal to twice the volume of aqueous KOH solution, was added.The mixture was stirred thoroughly and left in the ice box overnight.It was then centrifuged, the supernatant liquid poured off, the precipitate washed once with 5 cc. of absolute alcohol, the tube centrifuged again, and the washings discarded.The glycogen precipitate was dissolved in boiling distilled water, the volume used varying from 2 to 5 cc., depending upon the amount of glycogen present, 2 drops of 2 N HzS04 were added to neutralize the alkali not removed by washing, and this was followed by the addition 535
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