Gonadal hormone regulation of the renal vasopressin V2 receptor
2008; Wiley; Volume: 22; Issue: S1 Linguagem: Inglês
10.1096/fasebj.22.1_supplement.1159.24
ISSN1530-6860
AutoresJun Liu, Hong Ji, Wei Zheng, Joseph G. Verbalis, Kathryn Sandberg,
Tópico(s)Birth, Development, and Health
ResumoThe renal vasopressin V2 receptor (V2R) plays an important role in the physiological and pathophysiological processes underlying renal escape from arginine vasopressin (AVP)‐induced antidiuresis. The V2R gene is located on the X chromosome and has a high probability of escaping X‐inactivation. Thus, we investigated whether sex differences in expression of the renal V2R exist. We found that kidney V2R mRNA expression was 2.7‐fold higher in female (F) compared to male (M) MF‐1 mice [arbitrary units (AU): F, 8.13 ± 1.64 vs M, 3.06 ± 0.99, n=3; p<0.05]. To determine if these sex differences were a result of gonadal hormone effects, we measured V2R expression 12 weeks after sham‐operation (Sham) or ovariectomy (OVX) in mouse kidneys. Ovariectomy increased V2R mRNA expression by 1.8‐fold in the renal cortex [AU: OVX, 0.58 ± 0.02 vs Sham, 0.32 ± 0.02, n=3; p<0.05] and by 2.2‐fold in the inner medulla [AU: OVX, 4.02 ± 0.85 vs Sham, 1.82 ± 0.13, n=3; p<0.05]. These studies indicate that the renal V2R is regulated by ovarian hormones in female mice. Furthermore, sex differences in renal V2R expression have implications for pathologies associated with aberrant regulation of the V2R, specifically that females may be less able to escape AVP‐induced antidiuresis because of higher renal V2R expression than males (supported by R01 HL57502).
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