Chapter X Disc Electrophoresis
1971; Academic Press; Linguagem: Inglês
10.1016/s0580-9517(08)70648-2
ISSN0580-9517
Autores Tópico(s)Nanopore and Nanochannel Transport Studies
ResumoThe technique of disc electrophoresis derives its name from the introduction of discontinuities into both gel matrix and buffer systems. Several supporting media have been used for electrophoretic separation of biological compounds and each has its particular advantage. The advantage of using a gel as compared to—for instance, paper, is that the matrix of the gel imparts a separation based on the size of the individual components of the mixture undergoing separation. This was recognized by Smithies who suggested that the remarkable resolution obtained in starch-gel electrophoresis was because of molecular sieving in the gel matrix. Starch gel is a good medium for electrophoresis, particularly in flat blocks. It does suffer from the disadvantage that it is difficult to make gels of large pore size and difficulty is experienced in slicing and handling gels of high-urea content. Starch also has a small number of ionizable groups that cause electroendosmosis during electrophoresis.
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