Validated high-performance liquid chromatographic assay for the determination of promazine in human plasma
1998; Elsevier BV; Volume: 719; Issue: 1-2 Linguagem: Inglês
10.1016/s0378-4347(98)00262-x
ISSN1872-812X
AutoresVéronique Larsimont, Jürgen Meins, Hiltrud Fieger-Büschges, Henning Blume,
Tópico(s)Antibiotics Pharmacokinetics and Efficacy
ResumoA high-performance liquid chromatographic method for the determination of promazine in human plasma is described. The assay involves a single-step liquid–liquid extraction using pentane–2-propanol (98:2, v/v). The analyte of interest and the internal standard chlorpromazine were separated on a Spherisorb CN column using a mobile phase of acetonitrile–50 mM ammonium acetate (9:1, v/v). Electrochemical detection was achieved using an applied potential of +750 mV. The assay was validated according to international requirements prior to application to a pharmacokinetic study and was found to be specific, accurate and precise with a linear range of 0.25–25 ng ml−1.
Referência(s)