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In vivo cloning of the pectate lyase and cellulase genes of Erwinia chrysanthemi

1985; Springer Nature; Volume: 4; Issue: 3 Linguagem: Inglês

10.1002/j.1460-2075.1985.tb03698.x

1460-2075

Frédérique Van Gijsegem, Ariane Toussaint, Eric Schoonejans,

Plant-Microbe Interactions and Immunity

Article1 March 1985free access In vivo cloning of the pectate lyase and cellulase genes of Erwinia chrysanthemi Frédérique van Gijsegem Frédérique van Gijsegem Laboratoire de Génétique, Département de Biologie Moléculaire, Université Libre de Bruxelles, Rhode-Saint-Genèse, Belgium Search for more papers by this author Ariane Toussaint Ariane Toussaint Laboratoire de Génétique, Département de Biologie Moléculaire, Université Libre de Bruxelles, Rhode-Saint-Genèse, Belgium Search for more papers by this author Eric Schoonejans Eric Schoonejans Laboratoire de Génétique, Département de Biologie Moléculaire, Université Libre de Bruxelles, Rhode-Saint-Genèse, Belgium Search for more papers by this author Frédérique van Gijsegem Frédérique van Gijsegem Laboratoire de Génétique, Département de Biologie Moléculaire, Université Libre de Bruxelles, Rhode-Saint-Genèse, Belgium Search for more papers by this author Ariane Toussaint Ariane Toussaint Laboratoire de Génétique, Département de Biologie Moléculaire, Université Libre de Bruxelles, Rhode-Saint-Genèse, Belgium Search for more papers by this author Eric Schoonejans Eric Schoonejans Laboratoire de Génétique, Département de Biologie Moléculaire, Université Libre de Bruxelles, Rhode-Saint-Genèse, Belgium Search for more papers by this author Author Information Frédérique Gijsegem1, Ariane Toussaint1 and Eric Schoonejans1 1Laboratoire de Génétique, Département de Biologie Moléculaire, Université Libre de Bruxelles, Rhode-Saint-Genèse, Belgium The EMBO Journal (1985)4:787-792https://doi.org/10.1002/j.1460-2075.1985.tb03698.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Using an RP4 plasmid which carries a mini-Mu prophage which allows it to integrate spontaneously random pieces of its host chromosome, we cloned in vivo at least some of the pectate lyase and cellulase genes of the Erwinia chrysanthemi strain B374. The RP4-prime plasmids were used to localize the cloned genes on the B374 chromosome by co-transposition mapping and to subclone most of the genes in a classic high copy number plasmid vector. Previous ArticleNext Article Volume 4Issue 31 March 1985In this issue RelatedDetailsLoading ...