Portal de Periódicos da CAPES

[31] Multistep enzyme systems

1976; Academic Press; Linguagem: Inglês

10.1016/s0076-6879(76)44033-8

1557-7988

Klaus Mosbach, Bo Mattìasson,

Amino Acid Enzymes and Metabolism

This chapter discusses the immobilization of multistep enzyme systems. There are two ways of creating an enzyme sequence: (a) by immobilizing the different enzymes on the same particle, thereby creating a multistep enzyme system, (b) by immobilizing the different enzymes on separate particles, subsequently to be either mixed in a bed or arranged in sequential reactors. Technical and analytical processes involving multistep enzyme catalysis may be performed in columns, hollow fibers, or so-called enzyme reactors. The different enzymes participating may either (a) be attached together to the same supporting particle or (b) be bound to separate particles. The method of choice in each individual case depends on specific parameters of the processes to be catalyzed and on the characteristics of the participating enzymes. Practical application of different approaches is discussed and illustrated with a few examples, whereas methodological emphasis is placed on the coimmobilization of sequential enzymes on the same particles.