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Functional expression of individual plasmid-coded RNA bacteriophage MS2 genes

1982; Springer Nature; Volume: 1; Issue: 2 Linguagem: Inglês

10.1002/j.1460-2075.1982.tb01148.x

1460-2075

Erik Remaut, P. De Waele, Anne Marmenout, Patrick Stanssens, Walter Fiers,

Genomics and Phylogenetic Studies

Article1 February 1982free access Functional expression of individual plasmid-coded RNA bacteriophage MS2 genes Erik Remaut Erik Remaut Laboratory of Molecular Biology, State University of Ghent, Ledeganckstraat, 35, B-9000 Ghent, Belgium Search for more papers by this author Peter De Waele Peter De Waele Laboratory of Molecular Biology, State University of Ghent, Ledeganckstraat, 35, B-9000 Ghent, Belgium Search for more papers by this author Anne Marmenout Anne Marmenout Laboratory of Molecular Biology, State University of Ghent, Ledeganckstraat, 35, B-9000 Ghent, Belgium Search for more papers by this author Patrick Stanssens Patrick Stanssens Laboratory of Molecular Biology, State University of Ghent, Ledeganckstraat, 35, B-9000 Ghent, Belgium Search for more papers by this author Walter Fiers Walter Fiers Laboratory of Molecular Biology, State University of Ghent, Ledeganckstraat, 35, B-9000 Ghent, Belgium To whom reprint requests should be sent. Search for more papers by this author Erik Remaut Erik Remaut Laboratory of Molecular Biology, State University of Ghent, Ledeganckstraat, 35, B-9000 Ghent, Belgium Search for more papers by this author Peter De Waele Peter De Waele Laboratory of Molecular Biology, State University of Ghent, Ledeganckstraat, 35, B-9000 Ghent, Belgium Search for more papers by this author Anne Marmenout Anne Marmenout Laboratory of Molecular Biology, State University of Ghent, Ledeganckstraat, 35, B-9000 Ghent, Belgium Search for more papers by this author Patrick Stanssens Patrick Stanssens Laboratory of Molecular Biology, State University of Ghent, Ledeganckstraat, 35, B-9000 Ghent, Belgium Search for more papers by this author Walter Fiers Walter Fiers Laboratory of Molecular Biology, State University of Ghent, Ledeganckstraat, 35, B-9000 Ghent, Belgium To whom reprint requests should be sent. Search for more papers by this author Author Information Erik Remaut1, Peter De Waele1, Anne Marmenout1, Patrick Stanssens1 and Walter Fiers1 1Laboratory of Molecular Biology, State University of Ghent, Ledeganckstraat, 35, B-9000 Ghent, Belgium The EMBO Journal (1982)1:205-209https://doi.org/10.1002/j.1460-2075.1982.tb01148.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The genes of the RNA-containing bacteriophage MS2 were individually inserted into thermoinducible expression plasmids under control of the phage λ PL promoter. Three phage-coded proteins (A-protein, coat protein, and replicase) were expressed at high efficiency. Induced cultures specifically complemented superinfecting amber mutants of phage MS2. Regulatory mechanisms operative during the natural infection cycle of the phage were reproduced by the plasmid expression system. Previous ArticleNext Article Volume 1Issue 21 February 1982In this issue RelatedDetailsLoading ...