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BIBTEX RIS

Ti plasmid and chromosomal ornithine catabolism genes of Agrobacterium tumefaciens C58

1983; American Society for Microbiology; Volume: 155; Issue: 1 Linguagem: Inglês

10.1128/jb.155.1.196-202.1983

ISSN

1098-5530

Autores

Christopher L. Schardl, Clarence I. Kado,

Tópico(s)

Photosynthetic Processes and Mechanisms

Resumo

The pTiC58 plasmid noc genes of Agrobacterium tumefaciens C58 code for nopaline oxidase (nocC), nopaline permease (nocP), the inducible periplasmic protein n1 (nocB), and a function(s) required for ornithine catabolism (nocA). In addition, strains C58 and Ach-5 of A. tumefaciens have chromosomal ornithine catabolism genes. The chromosomal orc gene codes for ornithine dehydrogenase. Strain C58 is normally orc, but orc+ mutants can be selected. We have characterized both chromosomal orc and pTiC58 nocA plasmid genes. Complementation of most chromosomal orc mutants by pTiC58 restored growth on both nopaline and L-ornithine but did not restore ornithine dehydrogenase activity. We conclude that ornithine is an intermediate of nopaline degradation and that the Ti plasmid and chromosome both code for ornithine-degradative enzymes. A model for nopaline catabolism is presented.

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